BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0048.0098.p (343 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yetM [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase an... 136 7e-32 PA2587 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and... 99 1e-20 SA2099 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and... 86 1e-16 BMEI1017 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase a... 80 5e-15 PA3328 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and... 80 7e-15 >BS_yetM [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Bsu Bacillus subtilis Length = 369 Score = 136 bits (343), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 91/310 (29%), Positives = 142/310 (45%), Gaps = 5/310 (1%) Query: 8 RNYGAGISLGPATLRALKQLGVLEAFLREGAAGDGVRLCLPHGPQVAELPTPRLASPDVP 67 R GAGI L LK+LGV E + G + + G + ++ L +P Sbjct: 37 RKTGAGILQPQNALAVLKELGVFEDCCKHGFQTEWFKTFDEQGNLLFQVSESFL-DDSLP 95 Query: 68 GGGAILRPVLARILADATRAAGADVRLGCTFSALREVGHEVEVDFTDGQTRRYDLVIGAD 127 G ILR L IL A G D++ G A E V DG+ + D++ G D Sbjct: 96 GRNNILRKTLNDILMKHAEAVGVDIKWGKKVVAYEETAESVTALCEDGEKMQADILAGFD 155 Query: 128 GLYSKLRMHLFPHAPKPRYSGQAVWRAVLPRPQEIV--TCTMWMGPRIKPGVNPVSKDEM 185 G++S +R + + + G WR + P M+ + GV P+++ Sbjct: 156 GIHSVVRDKMLQKETEKEHLGMGAWRFYIELPDYTFEDATFMYRSGDTQIGVVPLAQHAG 215 Query: 186 YLFVTEPRPVNEHVDPATFVSHLRGLLEEFSAPVLKTIREQLDDNARIIFRPLEGLLLPR 245 Y+FV +P + + T ++ +L F L + + + +IF LE + + Sbjct: 216 YVFVLQPCTSDYWDEEDTRFDRVKEILSGFRG--LDFVTKHMSKQHPVIFNKLEQVAVQE 273 Query: 246 PWYQGRVVLIGDAVHATTPHLASGACIGIEDALVLADELERHGAAPQALAAFEERRWERC 305 PW++GRV++ GDA HA P LA GA + IEDA+VLA+EL+ H AL A+ +RR R Sbjct: 274 PWHKGRVIIGGDAAHAGAPTLAQGAAMAIEDAIVLAEELQNHADHETALQAYYKRRAPRA 333 Query: 306 RMVVENSARL 315 V S+ + Sbjct: 334 LKVQNLSSEI 343 >PA2587 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 382 Score = 99.4 bits (246), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 95/330 (28%), Positives = 147/330 (44%), Gaps = 24/330 (7%) Query: 1 VELDAQWRNYGAGISLGPATLRALKQLGVLEAFLREGAAGDGVRLCLPHGPQVAELPTPR 60 VE ++ R G GI+L L AL L+ R G G+ + G + +P+ Sbjct: 29 VERASEIRPIGTGITLASNALTALSSTLDLDRLFRRGMPLAGINVYAHDGSMLMSMPSS- 87 Query: 61 LASPDVPGGGAILRPVLARILADATRAAGADVRLGCTFSALREVGHEVEVDFTDGQTRRY 120 + GG A+ R L L + + +R+G + + + V +DG Sbjct: 88 -LGGNSRGGLALQRHELHAALLEGLDES--RIRVGVSIVQILDGLDHERVTLSDGTVHDC 144 Query: 121 DLVIGADGLYSKLRMHLFPHAPKPRYSGQAVWRAVLPRPQEIVTCT--MWMGPRIKPGVN 178 LV+GADG+ S +R +++P A R+SG+ WR V+P E +W G + G Sbjct: 145 SLVVGADGIRSSVRRYVWPEATL-RHSGETCWRLVVPHRLEDAELAGEVW-GHGKRLGFI 202 Query: 179 PVSKDEMYLFVT------EPRPVNEHVDPATFVSHLRGLLEEFSAPVLKTIREQLDDNAR 232 +S EMY++ T EP V P +H +F + +I + Sbjct: 203 QISPREMYVYATLKVRREEPEDEEGFVTPQRLAAHY----ADFDG-IGASIARLIPSATT 257 Query: 233 IIFRPLEGLLLPRPWYQGRVVLIGDAVHATTPHLASGACIGIEDALVLADELERHGAAPQ 292 ++ LE L W +GRVVLIGDA HA TP+L GA + +EDA +LA L + Sbjct: 258 LVHNDLEELA-GASWCRGRVVLIGDAAHAMTPNLGQGAAMALEDAFLLA-RLWCLAPRAE 315 Query: 293 ALAAFEERRWERCRMVVENS---ARLGEIE 319 L F+++R R + + S RLG+ E Sbjct: 316 TLILFQQQREARIEFIRKQSWIVGRLGQWE 345 >SA2099 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Sau Staphylococcus aureus N315 Length = 374 Score = 86.3 bits (212), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 60/219 (27%), Positives = 107/219 (48%), Gaps = 16/219 (7%) Query: 107 EVEVDFTDGQTRRYDLVIGADGLYSKLRMHLFPHAPKPRYSGQAVWRAV-----LPRPQE 161 +V + F + ++ +DL IGADG++SK+R + + K Y G +R + L P Sbjct: 128 KVTIHFAEQESEAFDLCIGADGIHSKVRQSVNADS-KVLYQGYTCFRGLIDDIDLKHPD- 185 Query: 162 IVTCTMWMGPRIKPGVNPVSKDEMYLFVTEPRPVNEHVDPATFVSHLRGLLEEFSAPVLK 221 + G + + G+ P+ ++ Y F+T N H + HL+ + V Sbjct: 186 --CAKEYWGRKGRVGIVPLLNNQAYWFITINSKENNHKYSSFGKPHLQAYFNHYPNEV-- 241 Query: 222 TIREQLDDNAR--IIFRPLEGLLLPRPWYQGRVVLIGDAVHATTPHLASGACIGIEDALV 279 RE LD + I+ + L + + GR +L+GDA HATTP++ GA +EDA+V Sbjct: 242 --REILDKQSETGILLHNIYDLKPLKSFVYGRTILLGDAAHATTPNMGQGAGQAMEDAIV 299 Query: 280 LADELERHGAAPQALAAFEERRWERCRMVVENSARLGEI 318 L + + +AL +++ R + V++ S ++G+I Sbjct: 300 LVNCFNAYD-FEKALQRYDKIRVKHTAKVIKRSRKIGKI 337 >BMEI1017 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Bme Brucella melitensis Length = 377 Score = 80.5 bits (197), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 73/257 (28%), Positives = 118/257 (45%), Gaps = 29/257 (11%) Query: 72 ILRPVLARILADATRAAGADVRLGCTFSALREVGHEVEVDFTDGQTRRYDLVIGADGLYS 131 ILR L RIL + + ++ G + + V V +D +T +IGADG+ S Sbjct: 106 ILRSDLLRILQEGN--SQDNLLYGKEVVEISQDAGRVSVALSDNETMETACLIGADGINS 163 Query: 132 KLRMHLFP-HAPKPRYSGQAVWRAVLPRPQEIVTC-TMWMGPRIKPGVNPVSKDEMYLFV 189 R F +A + WR + P P V C ++W+G + + PVS+ +Y++ Sbjct: 164 ITRTLAFNGNAVRAAALSNFSWRFMAPNPG--VDCWSLWVGSKSMILLIPVSEQSVYVWA 221 Query: 190 T---------EPRPVNEHVDPATFVSHLRGLLEEFSAPVLKTIREQLDDNARIIFRPLEG 240 T + R EH + F S LR ++E +KT ++ + PL+ Sbjct: 222 TLTGNRTDSGQVRLSGEHF--SDFPSDLRSIIEN----AVKTPQD-------VYNSPLQE 268 Query: 241 LLLPRPWYQGRVVLIGDAVHATTPHLASGACIGIEDALVLADELERHGAAPQALAAFEER 300 + + R W G V+L+GDA HAT P A GA +G+E AL LA + + P+ FE+ Sbjct: 269 VRMDR-WSAGNVLLLGDAAHATAPVWAQGAALGMEAALTLAKLVSQKVEQPELFDQFEKL 327 Query: 301 RWERCRMVVENSARLGE 317 R VV + ++ + Sbjct: 328 HRPRVNHVVAMTDKMSK 344 >PA3328 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 388 Score = 80.1 bits (196), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 90/316 (28%), Positives = 130/316 (41%), Gaps = 9/316 (2%) Query: 8 RNYGAGISLGPATLRALKQLGVLEAFLREGAAGDGVRLCLPHGPQVAELPTPRLASPDVP 67 R G+ +SL P L AL+++GV R A D +R G + + LA Sbjct: 41 RAVGSALSLMPNALTALERVGVRPDLTR-AQAFDSLRFLTRRGRPIRAIDFGGLARQLGQ 99 Query: 68 GGGAILRPVLARILADATRAAGADVRLGCTFSALREVGHEVEVDFTDGQTRRYDLVIGAD 127 AI R L + L + R ++ + T G V V +DG+ D++IGAD Sbjct: 100 PSLAIHRASLQQALLEQARDCRIELGVSATGYLRHADGEGVTVLCSDGREVHADVLIGAD 159 Query: 128 GLYSKLRMHLF-PHAPKPRYSGQAVWRAVLPRPQEIVT---CTMWMGPRIKPGVNPVSKD 183 G S +R + P P + +WRA VT + G + G+ + + Sbjct: 160 GFNSAIRATMTGPERPTDWH--YVIWRATPAFRHPKVTPGYVAHYWGRGQRFGLADIGEG 217 Query: 184 EMYLFVTEPRPVNEHVDPATFVSHLRGLLEEFSAPVLKTIREQLDDNARIIFRPLEGLLL 243 +Y + T P + D + ++ L ++ V I + A I P + Sbjct: 218 NVYWWGTRNMPAEQAKDWRGGKAGIQRLYAGWADEVQAVI--EATPEADISSLPAQDRPF 275 Query: 244 PRPWYQGRVVLIGDAVHATTPHLASGACIGIEDALVLADELERHGAAPQALAAFEERRWE 303 W G V L+GDA H L GA I IEDA VLA L AL A+E RR + Sbjct: 276 LERWGDGPVTLLGDAAHPMLTSLGQGAAIAIEDAAVLAHCLATIDDPQAALRAYENRRRD 335 Query: 304 RCRMVVENSARLGEIE 319 R R +VE S L IE Sbjct: 336 RARAMVETSRALSRIE 351