BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0048.0011.p (504 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO2122 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 454 e-127 MA4523 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 256 9e-68 VNG0872G [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A ... 228 3e-59 CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 221 3e-57 SPy1771 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 218 2e-56 >SSO2122 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Sso Sulfolobus solfataricus Length = 504 Score = 454 bits (1169), Expect = e-127, Method: Compositional matrix adjust. Identities = 228/501 (45%), Positives = 313/501 (62%), Gaps = 6/501 (1%) Query: 1 MSIVRPTPEQLKELASSLHIQLTTEQASQYLDLMQASFDAYDVIDGLPDEIPAVKYPRSA 60 M I PT E L+E++ ++ L E+ +L L++ ++Y+ +D LPD P VKY R+ Sbjct: 1 MGIKLPTLEDLREISKQFNLDLEDEELKSFLQLLKLQLESYERLDSLPDYTPRVKYQRTL 60 Query: 61 AARPAAKDNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSY 120 P ++NP A +++KG EGKL G+ + +KDNV +AG+PM+NGS LEGFVP Sbjct: 61 GRPPMKEENPYGALVWITSIKGKEEGKLKGKRICIKDNVMIAGIPMLNGSKMLEGFVPHM 120 Query: 121 DATIVTRLLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVA 180 DAT+V+R+LD I+ K TCE C SGGSHTS P PV NPR Y +GGSSSGSA VA Sbjct: 121 DATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNPRNPEYMAGGSSSGSAVAVA 180 Query: 181 AGEADMAIGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDN 240 +G DMA+GGDQGGSIRIPS++ G YG+K THGLVPYTG IE T+DH+GP+ V+D Sbjct: 181 SGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTHGLVPYTGAFSIEPTLDHLGPMANTVKDV 240 Query: 241 ALMLEVIAGADGLDPRQ----YAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVAD 296 AL+LEVIAG D LD RQ P V+PY+ +D V+ +K+GI+ EGF N +K V + Sbjct: 241 ALLLEVIAGRDELDSRQPDSLPPPPVKPYSKLIDGDVKDMKVGIVKEGFNWSNSEKDVDE 300 Query: 297 KVQEAIAQLEKLGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLG 356 V ++ +LE G V S+ H + +W PI EG T M+ G+G+G+ KGL++ Sbjct: 301 LVLDSAKKLEDYGIKVEDTSIPLHRMGLDIWTPIAIEGATATMILGSGVGWGRKGLFETQ 360 Query: 357 LLQHHAH-WREHADDLSATLKLCMFVGQYGLTRYQGRFYAKAQNIARRARTGYDKALADV 415 + + + A DL T+K + +G + Y R+YAKA+N++ + YD AL Sbjct: 361 IADFFGNSLKSRARDLPNTVKGVLMLGYLMIKMYNNRYYAKARNLSIVLKEAYDSALRKY 420 Query: 416 DLLVMPTVPIIAQPLPAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVG 475 D L+MPT P+ A + EY A MI NTAP D+TGHPAM+IP G +GLPVG Sbjct: 421 DALIMPTTPMKAMRYKSEPG-FDEYFIMALGMINNTAPFDVTGHPAMNIPVGYSNGLPVG 479 Query: 476 LMLVGKHYAEATLYQAAAAFE 496 LM++G+H+ E + + A FE Sbjct: 480 LMIIGRHFEEDKVLKLANVFE 500 >MA4523 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Mac Methanosarcina acetivorans str.C2A Length = 476 Score = 256 bits (654), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 157/420 (37%), Positives = 232/420 (55%), Gaps = 12/420 (2%) Query: 85 EGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTRLLDAGATILGKATCEHF 144 EG LAG + +KDN+++ G+P GS LEG+VP ++A ++ +LLDAGA ILGK + F Sbjct: 59 EGPLAGVPIAIKDNISVVGLPNSCGSKILEGYVPPFNAHVIEKLLDAGAVILGKTNLDEF 118 Query: 145 CLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMAIGGDQGGSIRIPSAFCG 204 + + TS P NP GGSS GSAA+VAAGEA A+G D GGS+R P+AFCG Sbjct: 119 AMGSSTETSYYGPTANPWDLERVPGGSSGGSAAVVAAGEAPFALGSDTGGSVRCPAAFCG 178 Query: 205 TYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLDPRQYAPEVQP 264 G+K T+G V GV+ +++ VGP+ NV D A++++VIAG D D + + Sbjct: 179 VVGLKPTYGAVSRYGVVAYANSLEQVGPLANNVEDIAILMDVIAGYDRRDSTSIDSKTE- 237 Query: 265 YTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVATVSVEEHALAG 324 Y AL V+GLKIG+ E F G + V V AI + E LGA+ VS+ A Sbjct: 238 YQKALVDDVKGLKIGVPKEFFGEG-IHPGVEKAVWNAIHKFESLGATRQEVSMPNINYAL 296 Query: 325 ALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREHADDLSATLKLCMFVGQY 384 A + I + + +G + ++ G H + A+ +K + +G Y Sbjct: 297 ASYYIIAMSEASSNLARFDGTRYGFRA---NGENWHAMVSKTRAEGFGTEVKRRILLGTY 353 Query: 385 GLTR-YQGRFYAKAQNIARRARTGYDKALADVDLLVMPTVPIIAQPL-PAAGCPLAEYVA 442 L+ Y ++Y KA + + +DKAL+ VDLL+ PT+P A + PL Y++ Sbjct: 354 ALSAGYHDKYYLKALKVRTLVKQDFDKALSTVDLLMAPTMPNPAFRIGEKIEDPLTLYLS 413 Query: 443 RAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVGKHYAEATLYQAAAAFEASMDWQ 502 NT P+++ G P++S+PCG DGLPVGL ++GK + E T+ +AA FE + D+ Sbjct: 414 DV-----NTCPINLAGVPSVSVPCGFTDGLPVGLQIMGKPFDEPTVLRAAYTFEKNTDYH 468 >VNG0872G [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Hbs Halobacterium sp. NRC-1 Length = 423 Score = 228 bits (581), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 157/432 (36%), Positives = 233/432 (53%), Gaps = 18/432 (4%) Query: 71 LNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTRLLD 130 LNA+ ++T++GAA+G L G TV +KDN++ GVP GS L + P Y+AT+V LL Sbjct: 3 LNAFITETTIQGAADGPLDGTTVAVKDNISTKGVPTTCGSKMLADYEPPYNATVVEDLLA 62 Query: 131 AGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMAIGG 190 AG TI+GK + F + + TS P NP GGSS GSAA VA G+AD+A+G Sbjct: 63 AGGTIVGKTNMDEFGMGTTTETSYFGPTKNPVDETRVPGGSSGGSAAAVANGDADLALGS 122 Query: 191 DQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGA 250 D GGS+R P+A+CG G+K T+GLV G++ +++ +GPI V A +L+VIAG Sbjct: 123 DTGGSVRAPAAYCGVVGLKPTYGLVSRYGLVAYANSLEQIGPIAPTVEGAAELLDVIAGP 182 Query: 251 DGLD-PRQYAPEVQPYTAALDRGVRGLKIGI---LTEGFALGNMDKRVADKVQEAIAQLE 306 D D + A Y +A V GL IG+ LT+G D RV ++ + A+A L Sbjct: 183 DEHDGTTRDAGADADYASAATGDVDGLTIGVPQELTDG-----ADDRVVERFEAALADLR 237 Query: 307 KLGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWRE 366 GA+ V++ A A + I + + +G+ + G +D + A R Sbjct: 238 AEGATTVDVALPSCEYAVAAYYVIAMSEASSNLARFDGVRYGTGGGFDGNWNETFADAR- 296 Query: 367 HADDLSATLKLCMFVGQYGLTR-YQGRFYAKAQNIARRARTGYDKALADVDLLVMPTVPI 425 AD + + +G Y L+ Y ++Y +AQ + +D+ ADVD++ PT+P+ Sbjct: 297 -ADGFGDEVTRRILLGTYALSAGYHDKYYKQAQEARAWVKQDFDETFADVDVVASPTMPV 355 Query: 426 IAQPL-PAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVGKHYA 484 + L + P+ Y+A A NT P ++ PA+S+P G DGLPVG+ LVG + Sbjct: 356 LPPKLGESLDDPVQMYLADA-----NTTPANLANLPAISVPAGDADGLPVGVQLVGPKFG 410 Query: 485 EATLYQAAAAFE 496 E T+ AAAA E Sbjct: 411 EETIINAAAAVE 422 >CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Cac Clostridium acetobutylicum Length = 478 Score = 221 bits (563), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 154/502 (30%), Positives = 245/502 (48%), Gaps = 33/502 (6%) Query: 1 MSIVRPTPEQLKELASSLHIQLTTEQASQYLDLMQASFDAYDVIDGLPDEIPAVKYPRSA 60 M I+ T EQL+ H++ D+++A FD ++ P EI A Sbjct: 1 MEILDMTVEQLRNAILDKHLKSE--------DIVKAYFD--NIKRNEP-EINAYITLCED 49 Query: 61 AARPAAKDNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSY 120 A AKD K G G+LAG + +KDN+ G+ S L FVP Y Sbjct: 50 YALKEAKD------VDKKIANGDKVGRLAGIPIAIKDNICTDGIKTTCASKMLYDFVPPY 103 Query: 121 DATIVTRLLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVA 180 DAT++ +L A I+GK + F + + S NPR GGSS GSAA+VA Sbjct: 104 DATVIKKLKAEDAIIIGKVNMDEFAMGSSTENSAFKITKNPRDITRVPGGSSGGSAAVVA 163 Query: 181 AGEADMAIGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDN 240 A A +++G D GGSIR P+AFCG G+K T+GLV G++ +++D +GP+ V+D Sbjct: 164 AKMAPISLGSDTGGSIRQPAAFCGVVGLKPTYGLVSRFGLIAFASSLDQIGPLGKTVKDC 223 Query: 241 ALMLEVIAGADGLDPRQYAP-EVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQ 299 A +LEVI+G D LD E + Y +D G++G+KIG+ E F +D + + Sbjct: 224 AELLEVISGEDELDNTSSKKHEKEDYLEGIDDGIKGMKIGMPKE-FLNDGLDPEIRKCID 282 Query: 300 EAIAQLEKLGASVATVS--VEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGL 357 + I +L+ LGA V +S + E L+ + G G+ D+ Sbjct: 283 DTIEKLKSLGAEVCEMSLPITEEGLSAYYIISSAEASSNLARFDGIRYGYRPDDFEDVYD 342 Query: 358 LQHHAHWREHADDLSATLKLCMFVGQYGLTR-YQGRFYAKAQNIARRARTGYDKALADVD 416 L + D+ +K + +G Y L+ Y +Y +A + ++ + + +A + D Sbjct: 343 LMETSRSEAFGDE----VKRRIMLGTYALSSGYYDAYYKRALKLKKKIKNEFKEAFENYD 398 Query: 417 LLVMPTVPIIAQPL-PAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCG-TVDGLPV 474 L++ P P++ + PL Y+A + T +++ G PA+S+PC + +GLP+ Sbjct: 399 LILSPVSPVLPFKIGEKKADPLQMYLADIY-----TVNINLAGIPAISLPCSVSKEGLPI 453 Query: 475 GLMLVGKHYAEATLYQAAAAFE 496 GL L+G H+ E +++AA A E Sbjct: 454 GLQLLGPHFGEKKIFRAARALE 475 >SPy1771 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Spy Streptococcus pyogenes M1 GAS Length = 488 Score = 218 bits (555), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 133/423 (31%), Positives = 215/423 (50%), Gaps = 10/423 (2%) Query: 84 AEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTRLLDAGATILGKATCEH 143 A+ ++G + +KDN++ + S L + P ++AT V ++GK + Sbjct: 65 ADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFNATSVANAYAKDMIVIGKTNMDE 124 Query: 144 FCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADMAIGGDQGGSIRIPSAFC 203 F + G + TS N H GGSS GSA VA+G+ +++G D GGSIR P+AF Sbjct: 125 FAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAVASGQVRLSLGSDTGGSIRQPAAFN 184 Query: 204 GTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLDPRQYAPEVQ 263 G+K T+G V G++ +++D +GP V++NA +L VIA +D D + Sbjct: 185 SVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTVKENAQLLNVIASSDVKDATSAPVRIA 244 Query: 264 PYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVATVSVEEHALA 323 YT+ + R ++G+KI + E G +D + + V ++ Q E LGA+V VS+ Sbjct: 245 DYTSKIGRDIKGMKIALPKEYLGEG-IDPEIKETVLASVKQFEALGATVEEVSLPHSKYG 303 Query: 324 GALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREHADDLSATLKLCMFVGQ 383 A++ I + + +G+ + ++ L + + + R +K + +G Sbjct: 304 VAVYYIIASSEASSNLQRFDGIRYGFRADDAKNLDEIYVNTRSQG--FGDEVKRRIMLGT 361 Query: 384 YGLTR-YQGRFYAKAQNIARRARTGYDKALADVDLLVMPTVPIIAQPLPAAG-CPLAEYV 441 + L+ Y ++ KA + +DK AD DL++ PT P +A L P+A Y+ Sbjct: 362 FSLSSGYYDAYFKKAGQVRTLIIQDFDKVFADYDLILGPTTPTVAFGLDTLNHDPVAMYL 421 Query: 442 ARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVGKHYAEATLYQAAAAFEASMDW 501 A T P+++ G P +SIP G VDGLPVGL L+G YAE T+YQAAAAFEA D+ Sbjct: 422 ADLL-----TIPVNLAGLPGISIPAGFVDGLPVGLQLIGPKYAEETIYQAAAAFEAVTDY 476 Query: 502 QQH 504 + Sbjct: 477 HKQ 479