BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0044.0031.p (354 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1389 [R] COG2220 Predicted Zn-dependent hydrolases of the bet... 323 3e-88 YPO1228 [R] COG2220 Predicted Zn-dependent hydrolases of the bet... 286 6e-77 XF2024 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 221 1e-57 BH3116 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 216 7e-56 PA3969 [R] COG2220 Predicted Zn-dependent hydrolases of the beta... 215 2e-55 >RSp1389 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Rso Ralstonia solanacearum Length = 362 Score = 323 bits (829), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 168/344 (48%), Positives = 211/344 (61%), Gaps = 28/344 (8%) Query: 17 DPSKSNRTDSGFRNNYAQPER---GSFWKWQWERLTQGLPKPPENHYQFPVLKPDLAWLK 73 +PSK + T GFRN Y R GS WKW ER GLPKPP + PDLA ++ Sbjct: 7 EPSKPHHTPDGFRNRYPHAPRDSNGSAWKWMRERRRLGLPKPPTA--DLSCVPPDLAAIR 64 Query: 74 DNDRRPSVTWIGHATALVQLGGKNVLTDPMFSQRASPFSFIGPARKVPLPVQISELPHID 133 P VTWIGH T L+Q+ G NVLTDP+F +RASP F GP R P + +++LPHID Sbjct: 65 RPSAVPQVTWIGHETLLLQIDGLNVLTDPVFGERASPLPFAGPRRHQPPGLSLAQLPHID 124 Query: 134 VVVISHNHYDHLDRASVEQLNLQPGGPPLFLVPLGLRDWFQ--------ALGITNVQEMD 185 +V++SHNHYDHL RASV L QPGG P+F VPLG+ WF+ + NV+ D Sbjct: 125 LVLLSHNHYDHLCRASVRALMRQPGGAPMFFVPLGIDRWFKRHVRGTRLSGEHVNVRAFD 184 Query: 186 WWDKTSLEQLDIHFVPATHWSARGLFDRSETLWGGWVLQAMDCPGKADTTRQSPVAPFSV 245 W + +HF HWSARGL+DR+ TLWG W L Sbjct: 185 WDTEARFGPFSVHFCAVQHWSARGLYDRNRTLWGSWALLHPQ---------------LRF 229 Query: 246 YFAGDTGYSQDFRDIGERFGHFDLALIPIGAYAPRWFMQNQHVDPAQAVLIHQDVHARRS 305 +F+GD GYSQD RDIG RFGHFD+A I +GAY PRWFM+ QH+DPA+AV + QD+ AR + Sbjct: 230 WFSGDLGYSQDTRDIGARFGHFDVAAIAVGAYEPRWFMKTQHIDPAEAVQVMQDIGARHA 289 Query: 306 VAIHWGTFELADDALDEPPKELAKAMQAAGLPQEQFVTLKHGET 349 V IHWGTF L D+ LD+P +LA A++A + E+FV +HGET Sbjct: 290 VGIHWGTFPLTDEPLDQPIADLANALRARDIDAERFVLFRHGET 333 >YPO1228 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Ype Yersinia pestis Length = 342 Score = 286 bits (731), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 155/364 (42%), Positives = 222/364 (60%), Gaps = 34/364 (9%) Query: 1 MLATSLGA----CSSSGKYADPSKSNRTDSGFRN---NYAQPERGSFWKWQWERLTQGLP 53 + AT++G+ + Y D SK + T+ GF+N P+ +W+ ER Q LP Sbjct: 2 LAATAVGSKGRKMAKKNPYYDASKPHHTEFGFQNIEPVVHHPQ--DLKRWREERKRQSLP 59 Query: 54 KPPENHY-QFPV---LKPDLAWLKDNDRRPSVTWIGHATALVQLGGKNVLTDPMFSQRAS 109 KPP+ Y QF + D + L+D ++ W+GHA+ L+++ G+ VL DP+ S R S Sbjct: 60 KPPQQGYPQFVTDWWQQADFSGLQD-----ALWWLGHASLLLRISGQAVLFDPVLSSRVS 114 Query: 110 PFSFIGPARKVPLPVQISELPHIDVVVISHNHYDHLDRASVEQLNLQPGGPPLFLVPLGL 169 P +F GP RK P+P + +LP IDV+VISHNHYDHLD ++ QL L+ +FLVPLGL Sbjct: 115 PVNFYGPQRKTPIPASVQQLPDIDVIVISHNHYDHLDVTTITQL-LRRFPDVVFLVPLGL 173 Query: 170 RDWFQALGITNVQEMDWWDKTSLEQLDIHFVPATHWSARGLFDRSETLWGGWVLQAMDCP 229 ++W + G +V E+DWWD+ + + H VPA HWS R +DR+ +LW GWV++ + Sbjct: 174 KNWLRQHGARHVHELDWWDQRLVADFEFHCVPARHWSMRTPWDRNHSLWSGWVVKRGEV- 232 Query: 230 GKADTTRQSPVAPFSVYFAGDTGYSQDFRDIGERFGHFDLALIPIGAYAPRWFMQNQHVD 289 + YF+GDTGY Q DIGE G F+ A +PIGAYAPRWFMQ QH+D Sbjct: 233 --------------NFYFSGDTGYCQQLLDIGEALGPFNYAALPIGAYAPRWFMQAQHMD 278 Query: 290 PAQAVLIHQDVHARRSVAIHWGTFELADDALDEPPKELAKAMQAAGLPQEQFVTLKHGET 349 P Q+V + Q +H ++ IHWG FELAD+ LDEPP++L +A+ AG+ +FV +K G Sbjct: 279 PQQSVQLFQKLHQPVTIPIHWGVFELADEPLDEPPEQLQQALTDAGVAHNKFVPIKIGAR 338 Query: 350 LKLD 353 + L+ Sbjct: 339 IILE 342 >XF2024 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Xfa Xylella fastidiosa 9a5c Length = 332 Score = 221 bits (564), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 130/318 (40%), Positives = 182/318 (57%), Gaps = 42/318 (13%) Query: 42 KWQWERLTQGLP---KPPENHYQFPVLKPDLAWLKDNDRRPSVTWIGHATALVQLGGKNV 98 +W+WE ++ LP P H P+ + + P +T IGH++ L+Q+ G N+ Sbjct: 37 RWRWE--SRHLPPWRDVPPTHVATPIAR---------SQHPIITMIGHSSVLIQIAGLNL 85 Query: 99 LTDPMFSQRASPFSFIGPARKVPLPVQISELPHIDVVVISHNHYDHLDRASVEQLNLQPG 158 L DP++S+R SP S++GP R + + LP ID V+I+HNHYDHLD ++ +L + Sbjct: 86 LVDPVWSERVSPLSWLGPRRWNSPGIDFNALPPIDYVLITHNHYDHLDLTTLARLQI--A 143 Query: 159 GPPLFLVPLG----LRDWFQALGITNVQEMDWWDKTSL--EQLDIHFVPATHWSARGLFD 212 P + PLG +R L ++ +DWWD L E I VPA HWSARGL D Sbjct: 144 HAPQVVTPLGNDAIIRKRVPDL---HIHTLDWWDSIQLTNEPTKISVVPAYHWSARGLRD 200 Query: 213 RSETLWGGWVLQAMDCPGKADTTRQSPVAPFSVYFAGDTGYSQD--FRDIGERFGHFDLA 270 R LWGG+ L T Q S+Y AGDT Y F I +R G DLA Sbjct: 201 RCMALWGGFHLH----------TPQG-----SLYIAGDTAYGNGSIFHAIAQRCGAPDLA 245 Query: 271 LIPIGAYAPRWFMQNQHVDPAQAVLIHQDVHARRSVAIHWGTFELADDALDEPPKELAKA 330 ++PIGAYAPRWFM NQH+D +AV I DV A +++ +HWGTF L D+A++EPP LA+A Sbjct: 246 ILPIGAYAPRWFMHNQHIDAVEAVQIMCDVGAHQALGVHWGTFRLTDEAVEEPPTLLAQA 305 Query: 331 MQAAGLPQEQFVTLKHGE 348 +QA G+P +F+ + G+ Sbjct: 306 LQAKGIPATRFIAMTPGQ 323 >BH3116 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Bha Bacillus halodurans Length = 305 Score = 216 bits (549), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 112/280 (40%), Positives = 163/280 (58%), Gaps = 27/280 (9%) Query: 72 LKDNDRRPSVTWIGHATALVQLGGKNVLTDPMFSQRASPFSFIGPARKVPLP-VQISELP 130 LK N + S TWIGH++ L+Q+ G N+LTDP+F++ ++G ++++ P + I ELP Sbjct: 49 LKKNRTKTSYTWIGHSSFLLQMKGLNILTDPVFAK------YLGFSKRLISPGLSIDELP 102 Query: 131 HIDVVVISHNHYDHLDRASVEQLNLQPGGPPLFLVPLGLRDWFQALGITNVQEMDWWDKT 190 ID+VVISH HYDHL+ +++ L G P+F VP GL FQ G+ V+E +WWD Sbjct: 103 EIDIVVISHGHYDHLEFSTLRSLK----GEPIFYVPAGLNLLFQKKGLHRVEEANWWDTF 158 Query: 191 SLEQLDIHFVPATHWSARGLFDRSETLWGGWVLQAMDCPGKADTTRQSPVAPFSVYFAGD 250 + L IHFVPA HW+ R LFD + + WGGWVL++ + YF GD Sbjct: 159 QHQDLTIHFVPAQHWTRRSLFDTNTSHWGGWVLESDEETN---------------YFVGD 203 Query: 251 TGYSQDFRDIGERFGHFDLALIPIGAYAPRWFMQNQHVDPAQAVLIHQDVHARRSVAIHW 310 TGY + F++IG++F D +PIG+Y P WF H++P AV ++ A+ V +H+ Sbjct: 204 TGYFRGFQEIGKKFA-LDTVFMPIGSYEPEWFTYASHINPEDAVKAFVELQAKTFVPMHY 262 Query: 311 GTFELADDALDEPPKELAKAMQAAGLPQEQFVTLKHGETL 350 GT+ LADD E L + LP+ Q +K GET+ Sbjct: 263 GTYRLADDTGPEALARLLHEWKRCQLPEHQLFVMKVGETV 302 >PA3969 [R] COG2220 Predicted Zn-dependent hydrolases of the beta-lactamase fold Pae Pseudomonas aeruginosa Length = 361 Score = 215 bits (547), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 134/354 (37%), Positives = 187/354 (52%), Gaps = 31/354 (8%) Query: 5 SLGACSSSGKYADPSKSNRTDSGFRN--NYAQPERGSFWKWQWERLTQGLPKPPENHYQF 62 +L C+ S Y P + FRN A KWQW R PP+ + Sbjct: 14 TLAGCAGSA-YQGPPSDHFDGERFRNIQPTAHKSLRDLLKWQWNR-------PPQRPWVA 65 Query: 63 --PVLKPDLAWLKDNDRRPSVTWIGHATALVQLGGKNVLTDPMFSQRASPFSFIGPARKV 120 P P + VT+I HAT L+Q G N+LTDP++SQR SP +FIGP R Sbjct: 66 LPPTATPPRVNERARGEELRVTYINHATLLIQHRGLNILTDPVWSQRVSPLAFIGPKRHH 125 Query: 121 PLPVQISELPHIDVVVISHNHYDHLDRASVEQLNLQPGGPPLFLVP-LGLRDWFQALGIT 179 P + + +LP ID+V++SHNHYDHLD ++ QL+ + P +V LG + G Sbjct: 126 PPGLTLDQLPPIDLVLVSHNHYDHLDLETLRQLHRR--WPTAKVVSGLGNAGLIRQTGFA 183 Query: 180 NVQEMDWWDKTSL-EQLDIHFVPATHWSARGLFDRSETLWGGWVLQAMDCPGKADTTRQS 238 +V E+DWW L L +H VP HWS+R D + TLW G+V+ + D P Sbjct: 184 DVVEIDWWQAVPLGAGLTLHGVPVQHWSSRTRSDTNRTLWLGFVIDSPDGP--------- 234 Query: 239 PVAPFSVYFAGDTGYSQDFRDIGERFGHFDLALIPIGAYAPRWFMQNQHVDPAQAVLIHQ 298 + F GDTG +F I +RFG A +PIGAY PRWFM++ H++P AV H+ Sbjct: 235 ------LLFPGDTGLGPEFGLINQRFGAMRFAALPIGAYEPRWFMRDHHMNPDDAVQAHR 288 Query: 299 DVHARRSVAIHWGTFELADDALDEPPKELAKAMQAAGLPQEQFVTLKHGETLKL 352 + A+ S+AIH+GTF L D+ D+PPK L A+++ G+ F T K GE ++ Sbjct: 289 TLQAQTSMAIHFGTFNLTDEQQDDPPKALQAALRSQGVDPATFGTPKPGEQWRV 342