BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0044.0010.p (369 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0589 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 340 4e-93 AGl3143 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 281 2e-75 BMEI1360 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A ... 253 6e-67 SMc02881 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A ... 228 2e-59 mll3370 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 226 9e-59 >RSc0589 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Rso Ralstonia solanacearum Length = 448 Score = 340 bits (871), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 196/369 (53%), Positives = 243/369 (65%), Gaps = 9/369 (2%) Query: 1 VAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYSGLGINPHYGTPQ 60 VAG+ T AGS L + A++ A V L AGA+L+G+TNM+EFA+SGLG+NPHYGTP+ Sbjct: 86 VAGQVTAAGSRALAHQSAATSDATAVARLRAAGAVLLGRTNMSEFAFSGLGLNPHYGTPR 145 Query: 61 NAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNGLTGFKPTAARV 120 +G R GGS+SG A++V GMA AA+G+DTGGS+RIP+A LTGFKPTA RV Sbjct: 146 TP----ADGTRAAGGSTSGGAVTVAGGMAVAALGTDTGGSIRIPAAFCALTGFKPTARRV 201 Query: 121 SMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGE--DYVAVLAHPLRGLRLLAPTNVVL 178 M G +PLS LDS GPLA SV CCAI+DAVL+G+ D AV PL GLRL + V Sbjct: 202 PMAGGVPLSTSLDSGGPLANSVDCCAIVDAVLSGQALDTDAV---PLAGLRLGLTRDYVA 258 Query: 179 DGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINAKGGFTAAEAYAWHRALIAE 238 +DDTV A+ A+ +L AGA IV E P L IN GG AAEA+AWHR +A Sbjct: 259 ADLDDTVATAFARAVAQLERAGAHIVRFEFPELLQLPEINGGGGLPAAEAWAWHRPHLAR 318 Query: 239 NAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQLAGYDALVMPTVPIVAPRI 298 A YD+RV +RI RG+ MSAA LDV+ AR R IA +L DA +MPTV +V P + Sbjct: 319 AEAQYDRRVAARIRRGEQMSAAAYLDVMAARARMIAAARKRLGNLDAWLMPTVAVVPPEV 378 Query: 299 AELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGSAPVGLSLAACGGQDKRLLS 358 A L+A D ++ N ++LRNP+ INFLDGCAL+LP HA G PVGLSL D R+L Sbjct: 379 APLEADDARFFHTNARVLRNPSAINFLDGCALTLPIHAAGELPVGLSLCGLADDDARILR 438 Query: 359 IGLAVEALL 367 +G AVE L Sbjct: 439 VGRAVETAL 447 >AGl3143 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 403 Score = 281 bits (719), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 161/368 (43%), Positives = 221/368 (60%), Gaps = 5/368 (1%) Query: 3 GETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYSGLGINPHYGTPQNA 62 G T AGS VL AS A VV L +AG + IG+TNM+EFA+SGLGINPHYGTP+N Sbjct: 35 GMPTTAGSKVLSDDAPASRDADVVAALKQAGMVSIGRTNMSEFAFSGLGINPHYGTPRNP 94 Query: 63 WERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNGLTGFKPTAARVSM 122 + +G R+PGGSSSGA + V G+ A+G+DTGGSVRIP+A NG+ G+K + R SM Sbjct: 95 V--STDGHRLPGGSSSGAGVVVAAGLVPVAIGTDTGGSVRIPAAFNGVVGYKASRGRYSM 152 Query: 123 QGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVAVLAHPLRGLRLLAPTNVVLDGMD 182 GV PL+ LDS+GPL +VQ +DA + G + PL G+ L+ P VV DG++ Sbjct: 153 HGVYPLAKSLDSLGPLTRTVQDAVWVDAAMRGRTAADLARMPLSGVSLVVPETVVFDGIE 212 Query: 183 DTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAA-INAKGGFTAAEAYAWHRA-LIAENA 240 V A+ A+ +L AGA + P F+ L I KG AEA++ H+A L +A Sbjct: 213 PGVAAAFEQAVERLVRAGASVRRQAFPVFSELFDLIREKGALVTAEAFSLHKARLEGADA 272 Query: 241 AGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQLAGYDALVMPTVPIVAPRIAE 300 A D RVV R G ++S D + ++ AR R +A A ++ + L+ PT+P VA ++A Sbjct: 273 ARMDPRVVVRTRLGANISMPDYIAIIEARDRMMAAFSAMISRDEILISPTLPHVAAKVAP 332 Query: 301 LQASDDAYYGANGKMLRNPTLINFLDGCALSLPC-HAPGSAPVGLSLAACGGQDKRLLSI 359 L +DDA++ N K LRN + NFLD C +S+PC PVGL L+ G D RLL + Sbjct: 333 LVENDDAFFAMNAKTLRNTQIGNFLDHCGVSIPCGTGEAGMPVGLLLSGLYGTDDRLLGV 392 Query: 360 GLAVEALL 367 +A E ++ Sbjct: 393 AMAAEEII 400 >BMEI1360 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Bme Brucella melitensis Length = 471 Score = 253 bits (645), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 157/378 (41%), Positives = 214/378 (56%), Gaps = 25/378 (6%) Query: 1 VAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYSGLGINPHYGTPQ 60 VAGE TLAGSVV P A A +VQ L AGA++IGKT+MTEFA++ +G+NPHYG P Sbjct: 92 VAGEPTLAGSVVRHDAPPAGCDALIVQRLRNAGAVIIGKTHMTEFAFTPVGLNPHYGEPG 151 Query: 61 NAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNGLTGFKPTAARV 120 NA I+ RIPGGSSSGAA+SV +G + A+GSDTGGSVRIP+ALNGL GFKPTA R+ Sbjct: 152 NA----IDPARIPGGSSSGAAVSVAEGTSEIAIGSDTGGSVRIPAALNGLVGFKPTARRI 207 Query: 121 SMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVAVLAHPLRGLRLLAPTNVVLDG 180 ++G PL+ LDS+GPL +V + DAV+AGE + P+ GLR+ P + +L Sbjct: 208 PLEGAFPLAPSLDSVGPLTRTVADAILADAVMAGEKPILPEVLPVNGLRIALPKDYLLAD 267 Query: 181 MDDTVGQAYRTALGKLAAAGAMI----VEAEVPAFNALAAINAKGGFTAAEAYA--WHRA 234 M+ V + +L L AGA+I V+ + I + G A+ +A W Sbjct: 268 MEPDVAAHFEASLAALEKAGAIIADLAVDDLIGRLKEATRIGSIAGIEASHIHASTW--- 324 Query: 235 LIAENAAGYDQRVVSRILRGKDMSAADLLDVLHA----RPRWIAQVEAQLAGYDALVMPT 290 A D V R+ R + L+ HA R +++ +L+G+D P Sbjct: 325 -----LADLDANVDIRVKRPLSVRIKVPLEAYHALMETRKALAREMDERLSGFDMFATPA 379 Query: 291 VPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPG-SAPVGLSLAAC 349 PIVAP IA + ++ Y +LR+ + N D C+++LP PG P GL L A Sbjct: 380 TPIVAPTIASVSHDEEEYDRVENLLLRDTQVANQFDLCSITLPM--PGMKLPTGLMLTAR 437 Query: 350 GGQDKRLLSIGLAVEALL 367 DKRLL+ L++E LL Sbjct: 438 NDSDKRLLAAALSIEKLL 455 >SMc02881 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Sme Sinorhizobium meliloti Length = 453 Score = 228 bits (580), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 153/359 (42%), Positives = 214/359 (59%), Gaps = 7/359 (1%) Query: 1 VAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYSGLGINPHYGTPQ 60 +AG T AGSVVL A+A A V+ +L AG + IG+TNM+EFA+SGLGINPHYGTP+ Sbjct: 82 LAGSITTAGSVVLAEGSPAAADATVIADLGSAGMVSIGRTNMSEFAFSGLGINPHYGTPR 141 Query: 61 NAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNGLTGFKPTAARV 120 N R+ + RIPGGSSSG+A +V G+ A+G+DTGGSVRIP+A+ G+ G+K T R Sbjct: 142 NP--RSADVHRIPGGSSSGSAAAVAAGLVPLAIGTDTGGSVRIPAAMTGIVGYKATRGRY 199 Query: 121 SMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVAVLAHPLRGLRLLAPTNVVLDG 180 +M+GV PL+ LDS+GPL +VQ DA + G + + L ++ P +V D Sbjct: 200 AMKGVFPLAQSLDSLGPLCQTVQDAVWADAAMHGLTAPVIRRAEIADLSIVVPETIVFDD 259 Query: 181 MDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINAK-GGFTAAEAYAWHRALIA-E 238 + V A+ A+ +L AAGA + P+F + + A+ G AEAYA HR +A Sbjct: 260 AEAEVVAAFEEAIKRLEAAGAKVRRQAFPSFAEIFELTARHGALVTAEAYALHRERLAGP 319 Query: 239 NAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQLAGYDALVMPTVPIVAPRI 298 A D RVV+R G+ ++ +D + +L AR R I + L + + PT+P VAP + Sbjct: 320 EAERMDPRVVARTRLGEKITVSDYIALLDARDRLIHETIETLKAGELIAHPTLPHVAPPL 379 Query: 299 AELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGSA--PVGLSLAACGGQDKR 355 L A DD ++ N + LRN ++ NFLD C +S+PC G+A P G LAA QD R Sbjct: 380 DPLLADDDLFFKVNARTLRNTSIGNFLDFCGVSIPC-GTGAAGMPAGFQLAAPHHQDDR 437 >mll3370 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Mlo Mesorhizobium loti Length = 431 Score = 226 bits (575), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 158/373 (42%), Positives = 208/373 (55%), Gaps = 15/373 (4%) Query: 1 VAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYSGLGINPHYGTPQ 60 VA E T AGS++ + A AA+V L +AGA++IGKTNMTEFA++ +G N HYGTP Sbjct: 66 VASEVTTAGSLMRKTAAPAMRDAAIVSRLRQAGAVIIGKTNMTEFAFTAIGDNMHYGTPG 125 Query: 61 NAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNGLTGFKPTAARV 120 NA I+ RIPGGSSSGA +SV +G + ++GSDTGGS+RIP++LN + GFKPTA RV Sbjct: 126 NA----IDASRIPGGSSSGAGVSVGEGTSDISIGSDTGGSIRIPASLNSVVGFKPTARRV 181 Query: 121 SMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVAVLAHPLRGLRLLAPTNVVLDG 180 + G PLS LDSIGPLA +V CA DAV+AGE + A L GLR+ P V+ Sbjct: 182 PLIGAFPLSGTLDSIGPLARTVAQCAATDAVMAGEAPAELAAISLAGLRVGVPRGVLFGD 241 Query: 181 MDDTVGQAYRTALGKLAAAGA----MIVEAEVPAFNALAAINAKGGFTAAEAYAWHRALI 236 + V A+ LGK+ AGA ++++ + F +G A E H + Sbjct: 242 TEGEVAAAFEHCLGKIEQAGARMADLVIDDMIAEFR---TATKRGSIAAMEGAEVHADWL 298 Query: 237 AENAA-GYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQLAGYDALVMPTVPIVA 295 A A+ D V + R + + VL R IA ++ +LA D L +PT P+ A Sbjct: 299 ATGASVPVDPHVSGPLSRAAMLPVPVYIRVLRRRSALIAAMDDRLASVDVLALPTTPVTA 358 Query: 296 PRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGSA-PVGLSLAACGGQDK 354 P I L + G +LRN + N D CA+SLP P A P GL L A G D+ Sbjct: 359 PTIVSLAEDAELRDHVEGLLLRNTQVANQFDLCAISLPM--PDMALPAGLMLVARNGHDR 416 Query: 355 RLLSIGLAVEALL 367 RLL I VE LL Sbjct: 417 RLLRIAAEVERLL 429