BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0307.p (236 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0258 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 257 2e-68 AF2407 [R] COG0491 Zn-dependent hydrolases, including glyoxylase... 70 4e-12 AF1748 [R] COG0491 Zn-dependent hydrolases, including glyoxylase... 68 2e-11 PAB0703 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 66 8e-11 PH1213 [R] COG0491 Zn-dependent hydrolases, including glyoxylase... 61 2e-09 >RSc0258 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Rso Ralstonia solanacearum Length = 319 Score = 257 bits (656), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 133/227 (58%), Positives = 156/227 (68%), Gaps = 12/227 (5%) Query: 8 IAATIHVLERGWLSSNNVLLFGRDE---VAMIDSGYVTHAEQTRTLVRHVLAQRGRPRLD 64 + T+ V ERGWLSSNN+L F D A++DSGY+THA QT L RH L +GRP L Sbjct: 10 LPGTMRVFERGWLSSNNILFFDDDAHGGCALVDSGYLTHAPQTVALARHAL--QGRP-LR 66 Query: 65 LLVNTHLHSDHCGGNAALQQEHHCRIAIPAAEEEKVRRWDEDALSYRATGQRCARFT--- 121 L+NTHLH+DHCGGNAALQ + CRI +PAA+ VRRWD DALSYR TGQ+C RFT Sbjct: 67 RLLNTHLHADHCGGNAALQAAYGCRIRVPAAQIGDVRRWDTDALSYRPTGQQCERFTLPE 126 Query: 122 ---ATQTLRSGEVLRMGELDWQVLAAPGHDPHALLFYCAQEGVLVSADALWRNGFGIIFP 178 A L G R+G LDWQV++APGHDPHA++ Y VL+SADALW GFG+IFP Sbjct: 127 GDTAEAGLADGMTARLGGLDWQVISAPGHDPHAVILYSPDTRVLLSADALWERGFGVIFP 186 Query: 179 ELDGQSGFAEARATLELIRSLEVRTVIPGHGAPFTEVDDARARARAR 225 EL+G+SGFAE A L I L R V+PGHG PFTEVD A AR R Sbjct: 187 ELEGESGFAEQEAILACIARLGARVVVPGHGRPFTEVDAAIDAARGR 233 >AF2407 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Afu Archaeoglobus fulgidus Length = 203 Score = 70.1 bits (170), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 50/193 (25%), Positives = 85/193 (44%), Gaps = 25/193 (12%) Query: 17 RGWLSSNNVLLFGRDEVAMIDSGYVTHAEQTRTLVRHVLAQRGRPRLDLLVNTHLHSDHC 76 R + S+N + +EVA+ID G R L+R G + + NTHLH DHC Sbjct: 14 RAFRDSSNCYVIADEEVAVIDPGTFKSYTNLRGLMR----NDGIGEANYVFNTHLHRDHC 69 Query: 77 GGNAALQQEHHCRIAIPAAEEEKVRRWDEDALSYRATGQRCARFTATQTLRSGEVLRMGE 136 N + R A+ L + + +++ ++ G+ +G Sbjct: 70 ESNVLFAE----RGAL---------------LGFDSKDMAVSQYRFNLDIKLGKHFIIGN 110 Query: 137 LDWQVLAAPGHDPHALLFYCAQEGVLVSADALWRNGFGIIFPELDGQSGFAEARATLELI 196 ++L PGH P +L FY + ++ D ++ NG F ++ G AR ++E + Sbjct: 111 TGIEILRTPGHSPGSLTFYLPEHSAAITGDLIFENGVPGRF-DIYGSDKKELAR-SIEKL 168 Query: 197 RSLEVRTVIPGHG 209 RSL+ ++PGHG Sbjct: 169 RSLDAEYILPGHG 181 >AF1748 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Afu Archaeoglobus fulgidus Length = 236 Score = 68.2 bits (165), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 106/240 (44%), Gaps = 25/240 (10%) Query: 8 IAATIHVLERGWLSSNNVLLFGRDEVAMIDSGYVTHAEQTRTLVRHVLAQRGR--PRLDL 65 I I+ ++ G LSS+ L+ A+IDSG A L R L + G +D+ Sbjct: 7 IHNNIYQIKPGRLSSHCYLITAELN-ALIDSGT---ARDFPKLERE-LGEIGLNAKDIDI 61 Query: 66 LVNTHLHSDHCGGNAALQQEHHCRIAIPAAEEE---KVRRWDEDALSYRATGQRCARFTA 122 ++NTH H DH GGN LQ+ +I A K+ D++ + R GQR + Sbjct: 62 VINTHEHFDHIGGNLFLQKN-----SIIMAHRHAAVKIIYGDDEVMMCRTHGQRPVGYRV 116 Query: 123 TQTLRSGEVLRMGELDWQVLAAPGHDPHALLFYCAQEGVLVSADALWRNGFGIIFPELDG 182 L + + + +G + +V+ PGH + Y + +L S D L+ NG + Sbjct: 117 HVWLNNIDAVDLGGVFLRVMHTPGHTSGCICLYDPRNRILFSGDTLFANG---TLSSIYN 173 Query: 183 QSGFAEARATLELIRSLEVRTVIPGHG--APFTEVDDARA-----RARARARARARAAGL 235 E +L I+++++ ++PGHG + EVD R R A A+ GL Sbjct: 174 SGSLGEYFNSLRKIKTMKIDLLLPGHGRISGNVEVDIERTLENTIRTFPDAEYIAKMLGL 233 >PAB0703 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Pab Pyrococcus abyssi Length = 222 Score = 65.9 bits (159), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 52/208 (25%), Positives = 94/208 (45%), Gaps = 15/208 (7%) Query: 8 IAATIHVLERGWLSSNNVLLFGRDEVAMIDSGYVTHAEQTRTLVRHVLAQRGRPRLDLLV 67 I I +LE L SN L +DE+ ++D+G + + R+ ++ ++ Sbjct: 6 IKPNILMLEGVNLDSNVYFLKSKDELLIVDTGTGVYWNKYLDTARNEGWLENVSKV-IIF 64 Query: 68 NTHLHSDHCGGNAALQQEHHC------RIAIPAAEEEKVRRWDEDALSYRATGQRCARFT 121 NTH H DH GGN +++ ++ A EE D LSY G+R Sbjct: 65 NTHEHFDHVGGNLVFKEKLKVVEFASHKLTAKALEEGD----DYIILSYYY-GRRYDPHE 119 Query: 122 ATQTLRSGEVLRMGELDWQVLAAPGHDPHALLFYCAQEGVLVSADALWRNGFGIIFPELD 181 L G+ +++G++ +++ PGH + Y +E ++ + D ++ +G Sbjct: 120 VELKLEDGDEIKVGKVKLRLIHTPGHTRGSSCLYYEEERIMFTGDTVFLGTYGRTDLPTG 179 Query: 182 GQSGFAEARATLELIRSLEVRTVIPGHG 209 + E+ LEL++S +VR +PGHG Sbjct: 180 NEDKIVES---LELLKSFDVRLGLPGHG 204 >PH1213 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Pho Pyrococcus horikoshii Length = 223 Score = 61.2 bits (147), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 36/147 (24%), Positives = 67/147 (45%), Gaps = 4/147 (2%) Query: 65 LLVNTHLHSDHCGGNAALQQE-HHCRIAIPAAEEEKVRRWDEDALSYRATGQRCARFTAT 123 ++ NTH H DH GGN +++ + + A + + D+ + G+R Sbjct: 63 IIFNTHEHFDHVGGNLIFKEKLRNVKFAAQRLTAKALEDGDDYVILSFYYGRRYEPHDVE 122 Query: 124 QTLRSGEVLRMGELDWQVLAAPGHDPHALLFYCAQEGVLVSADALWRNGFGIIFPELDGQ 183 L GE +R+G ++ +L PGH + Y +E ++ + D ++ +G + Sbjct: 123 LKLEDGEKIRIGNIELTLLHTPGHTRGSSCLYYKEEKIMFTGDTVFAGTYGRTDLPTGDE 182 Query: 184 SGFAEARATLELIRSLEVRTVIPGHGA 210 S E+ LE ++ +VR +PGHG Sbjct: 183 SKMIES---LEFLKDFDVRIGLPGHGK 206