BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0042.0027.p (315 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the T... 196 6e-50 AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the T... 116 7e-26 RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the T... 94 3e-19 Cgl0451 Non COG 78 2e-14 >YPO1933 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Ype Yersinia pestis Length = 289 Score = 196 bits (498), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 108/268 (40%), Positives = 148/268 (55%), Gaps = 2/268 (0%) Query: 50 LPDDACDCHMHIYDPRFAWAPGAKLVHAP--ATVAMYRQLQQRLGTTRNVVVTPSAYGVD 107 LP+DACD H H+ PR + A+ + P A Y + G +R V+V S YG D Sbjct: 22 LPEDACDSHAHVISPRAIYPMVAERSYTPPPAPEEKYLSMLAATGMSRGVLVQISVYGSD 81 Query: 108 NSCTLDALAQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLA 167 N L L + RGVAVV + V+D ELQ + AGVRGLR N+ G + ME LA Sbjct: 82 NRYMLQVLKRHPEYLRGVAVVTEDVTDQELQDMALAGVRGLRINVLFGGGIGFDAMENLA 141 Query: 168 RRVAPLGWHLQANMPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMR 227 ++APLGWH+Q M L A + +LP P V DH +P+ HP + + ++R Sbjct: 142 SKIAPLGWHMQFLMDVRQLPALIPRMKKLPCPCVIDHMGHMPVSLGLNHPGYQTLLHMVR 201 Query: 228 ERRASVKLSGAYLYSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARL 287 E VKLSGAY S+ Y DV P A+ L++ AP ++VWGSDWPH + PD L Sbjct: 202 EYGWWVKLSGAYRISESWQDGYLDVVPFAQKLIETAPDRMVWGSDWPHVSVSRMPDTGGL 261 Query: 288 LDVMISWLGSEEMVRTVLVANPARLYQF 315 +++ +W+ E++ +LV NPARLY F Sbjct: 262 RNLLAAWVPDEQVRNRILVDNPARLYDF 289 >AGl3341 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 292 Score = 116 bits (290), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 76/271 (28%), Positives = 123/271 (45%), Gaps = 6/271 (2%) Query: 50 LPDDACDCHMHIYDPRFAWAPGAKLVHAPATVAM--YRQLQQRLGTTRNVVVTPSAYGVD 107 P A D MH+Y P + PG + A YR+L Q LG R ++ +A+ D Sbjct: 17 FPRGAVDTQMHMYLPGYPALPGGPGLPPGALPGPEDYRRLMQWLGIDRVIITQGNAHQRD 76 Query: 108 NSCTLDALAQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLA 167 N TL +A++G +A V +++ ++ ++++L AG G R G + ++ + Sbjct: 77 NGNTLACVAEMGEAAHAVVIIDATTTEKDMEKLTAAGTVGARIMDLPGGAVNLSELDAVD 136 Query: 168 RRVAPLGWHLQANMPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMR 227 R W + LL H L ++ VFDH + P + L+ Sbjct: 137 ERAHAADWMVAVQFDGNGLLDHLPRLQKIRSRWVFDHHGKFFKGIRTDGPEMAALLKLID 196 Query: 228 ERRASVKLSGAYLYSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHK---PDD 284 K +G Y S++ P Y DVA +R + AP ++VWG++WPH + + PDD Sbjct: 197 RGNLWFKFAGVYESSRKSWP-YADVAAFSRVIAAHAPERIVWGTNWPHNSVRETAAYPDD 255 Query: 285 ARLLDVMISWLGSEEMVRTVLVANPARLYQF 315 ARL ++ + WL E LV NP L++ Sbjct: 256 ARLAELTLGWLPDEAARHRALVENPEALFKL 286 >RSp0233 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Rso Ralstonia solanacearum Length = 302 Score = 94.4 bits (233), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 87/270 (32%), Positives = 121/270 (44%), Gaps = 13/270 (4%) Query: 54 ACDCHMHIYDPRFAWAPGAKLVHAP---ATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSC 110 D H H++ A GA+ +AP AT+ Y G + V++ PS G DNS Sbjct: 37 GIDTHAHVFKRGLPLA-GARR-YAPSYDATLPAYLAQLDAHGLSHGVLIQPSFLGGDNSY 94 Query: 111 TLDALAQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRFNL--AVGSVTTVEMMEPLAR 168 L AL Q + RGVAV++ + L +L+ G+ G+R NL A + Sbjct: 95 LLAALRQAPQRLRGVAVIDPAAPEPFLAQLNAEGIVGIRLNLIGAPDPQLRAPAWQAALH 154 Query: 169 RVAPLGWHLQANMPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRE 228 R+ LGWH++ + L A L V +V DHF R P F + R Sbjct: 155 RLHALGWHVELHTQAHRLPALLPPLLEAQVDVVVDHFGRPDPALSVEDPGFAALLAAGRT 214 Query: 229 RRASVKLSGAYLYSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKP--DDAR 286 RR VK+SGAY G + +A + R L +LVWGSDWPH + + D AR Sbjct: 215 RRVWVKVSGAYRNGDAGRGEAIALAAMPRLKDALGTDRLVWGSDWPHTQYESRIGYDSAR 274 Query: 287 -LLDVMISWLGSEEMVRTVLVANPARLYQF 315 D ++ G VLV PARL++F Sbjct: 275 AFADTLLPDAGDRHQ---VLVETPARLFRF 301 >Cgl0451 Non COG Length = 250 Score = 78.2 bits (191), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 76/259 (29%), Positives = 108/259 (41%), Gaps = 19/259 (7%) Query: 56 DCHMHIYDPRFAWAPGAKLVHAPATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDAL 115 D H HI DP+ + P TV Y + L +V+ S D DAL Sbjct: 6 DSHFHIIDPQHPLIENNGYLPEPFTVEDYTARVEGLEVAGGAIVSGSFQAFDQGYLKDAL 65 Query: 116 AQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLARRVAPL-G 174 A LG GV + SD E+ L +AGV+ +R NL G ++ +E LARRV L G Sbjct: 66 AVLGPGYVGVTQIPADTSDQEILDLDKAGVKAVRLNLKRGGSAGLDDLETLARRVHDLAG 125 Query: 175 WHLQANMPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVK 234 WH + + L + LA LP + DH L D + V E VK Sbjct: 126 WHTELYVDARELDELESTLASLPA-VSIDHLG---LHRDGLPALLRLV-----ENGIKVK 176 Query: 235 LSGAYLYSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISW 294 +G D + +A++ + PT L+ G+D P T +P + LD++ Sbjct: 177 ATGFGRVEL-------DPTEVIQAIMAVDPTALMIGTDLP-STRTKRPFEDADLDLIAET 228 Query: 295 LGSEEMVRTVLVANPARLY 313 +G E+ V V N A Y Sbjct: 229 VG-EDHVDNVFWNNAAAFY 246