BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0041.0200.p (103 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1254 [I] COG3000 Sterol desaturase Rso Ralstonia solanacearum 74 6e-14 >RSc1254 [I] COG3000 Sterol desaturase Rso Ralstonia solanacearum Length = 324 Score = 74.3 bits (181), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 33/72 (45%), Positives = 49/72 (68%) Query: 1 MIQTISDLFAAVQGWLFQTLVQPLVFFIGMGEFVEEAFTGTEFVLVGLCELVILFLILRP 60 M ++D F Q LFQ +V PL + IGMG +VE+A+ GTE++L+GL ++ ++ L+LRP Sbjct: 1 MFDWVADTFTQAQDLLFQHVVMPLTYAIGMGGYVEDAYPGTEWLLMGLVQIAVMLLVLRP 60 Query: 61 LESAIPVHPITD 72 LE PV P+ D Sbjct: 61 LERWRPVEPMQD 72