BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0167.p (207 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0389 [L] COG0742 N6-adenine-specific methylase Rso Ralstonia ... 174 1e-43 VC0146 [L] COG0742 N6-adenine-specific methylase Vch Vibrio chol... 144 1e-34 PA0370 [L] COG0742 N6-adenine-specific methylase Pae Pseudomonas... 132 4e-31 YPO3816 [L] COG0742 N6-adenine-specific methylase Ype Yersinia p... 130 3e-30 yhhF [L] COG0742 N6-adenine-specific methylase Eco Escherichia c... 129 5e-30 >RSc0389 [L] COG0742 N6-adenine-specific methylase Rso Ralstonia solanacearum Length = 215 Score = 174 bits (441), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 105/204 (51%), Positives = 130/204 (63%), Gaps = 16/204 (7%) Query: 8 RGPHQVRIIGGQWKRTPLPVLDAEGLRPTPDRVRETVFNWITHLIDGNWEAVSCLDLFAG 67 R PHQVRIIGGQ+KRTPLPV+DAEGLRPT DRVRET+FNW+ + G W C DLFAG Sbjct: 19 RAPHQVRIIGGQYKRTPLPVIDAEGLRPTGDRVRETLFNWLGQDLAG-WR---CADLFAG 74 Query: 68 SGALGFEAASRGARRVVMVEHSGPAVRQLEANRDKLKAATLAILRGDALAAAQNAAQREP 127 +GALGFEAASRGA RV +VE PA+R L A RDKL+A + I+ GD A A++ Sbjct: 75 TGALGFEAASRGAARVTLVESHAPALRALHAVRDKLRAGMVDIVAGDVFAW---LARQAD 131 Query: 128 GGFQLVFIDPPYHQGWLEKVLPACQQLLAPTGLVYAEAEFALDGA--------DAPAWMQ 179 G F LVFIDPP+ Q W + L A +++ GL+Y E+ L +A + Sbjct: 132 GAFDLVFIDPPFAQDWTLRALEAALRVVPEGGLIYVESPRPLVAVEPGEGVAPEAGTPLP 191 Query: 180 AWEVV-RADKAGMVFYHLLQRKSG 202 A V+ R +AG V HLL RK+G Sbjct: 192 AGVVLHRHLRAGAVHAHLLLRKNG 215 >VC0146 [L] COG0742 N6-adenine-specific methylase Vch Vibrio cholerae Length = 199 Score = 144 bits (363), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 83/192 (43%), Positives = 110/192 (57%), Gaps = 13/192 (6%) Query: 12 QVRIIGGQWKRTPLPVLDAEGLRPTPDRVRETVFNWITHLIDGNWEAVSCLDLFAGSGAL 71 QVRII G W+ LPV DAEGLRPT DRV+ET+FNW+ + CLDLFAGSG L Sbjct: 18 QVRIISGLWRGRKLPVHDAEGLRPTTDRVKETLFNWLAQEVPN----ARCLDLFAGSGGL 73 Query: 72 GFEAASRGARRVVMVEHSGPAVRQLEANRDKLKAATLAILRGDALAAAQNAAQREPGGFQ 131 GFE ASR A +V M+E + A +QL+ N LKA + ++ DALA + + + + Sbjct: 74 GFECASRQANQVTMLELNPLAFQQLKTNVQSLKAENIELIHTDALAFLRQSGK----PYD 129 Query: 132 LVFIDPPYHQGWLEKVLPACQQ--LLAPTGLVYAEAEFALDGADAPAWMQAWEVVRADKA 189 +VFIDPP+ QG L++ +Q LAP ++Y E E L + P W + R A Sbjct: 130 VVFIDPPFRQGLLQETTQLLEQNGWLAPQAMIYIECEKELPLTELPP---HWHLHREKSA 186 Query: 190 GMVFYHLLQRKS 201 G V Y L R+ Sbjct: 187 GQVCYRLFIREE 198 >PA0370 [L] COG0742 N6-adenine-specific methylase Pae Pseudomonas aeruginosa Length = 198 Score = 132 bits (333), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 87/208 (41%), Positives = 110/208 (52%), Gaps = 24/208 (11%) Query: 3 SKPANRGPH------QVRIIGGQWKRTPLPVLDAEGLRPTPDRVRETVFNWITHLIDGNW 56 SKP + P Q+RIIGG+W+ D GLRPTPDRVRET+FNW+ ++G Sbjct: 2 SKPTAKAPGRHGGQGQLRIIGGEWRSRRFVFPDGPGLRPTPDRVRETLFNWLAPYVEG-- 59 Query: 57 EAVSCLDLFAGSGALGFEAASRGARRVVMVEHSGPAVRQLEANRDKLKAATLAILRGDAL 116 LD FAGSGAL EA SRGAR + ++ +G AV L + D LK T ++ GDA+ Sbjct: 60 --ARVLDPFAGSGALFLEALSRGAREGLALDTNGEAVAALRNHLDALKCGTAQLVLGDAV 117 Query: 117 AAAQNAAQREPGGFQLVFIDPPYHQGWLEKVLPACQQL-----LAPTGLVYAEAEFALDG 171 N A P F LVF+DPP+HQ L+ AC+ L L P +Y E+E Sbjct: 118 RYLGNQA---PSAFDLVFLDPPFHQNLLQD---ACRLLETRGWLNPDAWIYTESESVPSS 171 Query: 172 ADAPAWMQAWEVVRADKAGMVFYHLLQR 199 P W + R KAG V Y L QR Sbjct: 172 LGLPG---NWRLHREKKAGNVHYALWQR 196 >YPO3816 [L] COG0742 N6-adenine-specific methylase Ype Yersinia pestis Length = 220 Score = 130 bits (326), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 84/192 (43%), Positives = 107/192 (55%), Gaps = 15/192 (7%) Query: 12 QVRIIGGQWKRTPLPVLDAEGLRPTPDRVRETVFNWITHLIDGNWEAVSCLDLFAGSGAL 71 Q+RIIGG+W+ LPV D+ GLRPT DRVRET+FNW+ +I G CLD FAGSGAL Sbjct: 22 QIRIIGGKWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPMIQG----ARCLDCFAGSGAL 77 Query: 72 GFEAASRGARRVVMVEHSGPAVRQLEANRDKLKAATLAILRGDALAAAQNAAQREPGG-F 130 G EA SR A ++E +QL N LKA ++ ++L Q AQ PG F Sbjct: 78 GLEALSRYASETTLLEADRQVAKQLSDNLTLLKAENGQVVNTNSL---QWLAQ--PGQPF 132 Query: 131 QLVFIDPPYHQGWLEKVLPACQQL--LAPTGLVYAEAEFALDGADAPAWMQAWEVVRADK 188 LVF+DPP+ +G L + + +Q LA +Y EAE AD PA W + R Sbjct: 133 NLVFLDPPFRKGLLAETINLLEQFNWLAADAWIYVEAEAESAVADVPA---NWLLHREKI 189 Query: 189 AGMVFYHLLQRK 200 AG V Y L R+ Sbjct: 190 AGQVAYRLFIRQ 201 >yhhF [L] COG0742 N6-adenine-specific methylase Eco Escherichia coli K12 Length = 198 Score = 129 bits (324), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 82/206 (39%), Positives = 111/206 (53%), Gaps = 13/206 (6%) Query: 4 KPANRGPHQVRIIGGQWKRTPLPVLDAEGLRPTPDRVRETVFNWITHLIDGNWEAVSCLD 63 KP + G Q+RIIGGQW+ LPV D+ GLRPT DRVRET+FNW+ +I CLD Sbjct: 3 KPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVI----VDAQCLD 58 Query: 64 LFAGSGALGFEAASRGARRVVMVEHSGPAVRQLEANRDKLKAATLAILRGDALAAAQNAA 123 FAGSGALG EA SR A ++E +QL N LKA ++ +A++ A Sbjct: 59 CFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGNARVVNSNAMSFL--AQ 116 Query: 124 QREPGGFQLVFIDPPYHQGWLEKVLPACQQ--LLAPTGLVYAEAEFALDGADAPAWMQAW 181 + P +VF+DPP+ +G LE+ + + LA L+Y E+E PA W Sbjct: 117 KGTP--HNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEVENGLPTVPA---NW 171 Query: 182 EVVRADKAGMVFYHLLQRKSGARIEA 207 + R AG V Y L QR++ +A Sbjct: 172 SLHREKVAGQVAYRLYQREAQGESDA 197