BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0039.0132.p (462 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0494 [I] COG0439 Biotin carboxylase Pae Pseudomonas aeruginosa 711 0.0 NMB1861 [I] COG0439 Biotin carboxylase Nme Neisseria meningitidi... 474 e-133 BMEI1063 [I] COG0439 Biotin carboxylase Bme Brucella melitensis 470 e-132 NMA0596 [I] COG0439 Biotin carboxylase NmA Neisseria meningitidi... 469 e-132 AGc2451 [I] COG0439 Biotin carboxylase Atu Agrobacterium tumefac... 467 e-131 >PA0494 [I] COG0439 Biotin carboxylase Pae Pseudomonas aeruginosa Length = 458 Score = 711 bits (1835), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/452 (80%), Positives = 402/452 (88%) Query: 1 MTTSLRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARAD 60 M L KLL+ANRGEIAVRIIRAAQALGI TVA CS+AD DSLAAR+ADEV+ IGPARAD Sbjct: 1 MHKRLNKLLVANRGEIAVRIIRAAQALGIPTVAVCSEADRDSLAARLADEVRLIGPARAD 60 Query: 61 RSYLNVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKA 120 RSYL+++A+L AAR++GAD++HPGYGFLSEN AFAEAV AAGL+FVGP+A TIRRMGDKA Sbjct: 61 RSYLDIEAILMAARETGADSIHPGYGFLSENPAFAEAVQAAGLVFVGPEAQTIRRMGDKA 120 Query: 121 EARRTAAAAGVPVVPGSAGELEDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATEL 180 EARRTA AAGVPVVPGS GEL D+ ALACAEE+GYPLL+KASAGGGGRGIR+A DA EL Sbjct: 121 EARRTAMAAGVPVVPGSPGELGDLDAALACAEEVGYPLLVKASAGGGGRGIRIAHDADEL 180 Query: 181 AREFPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGERAVHLFERECSLQRRRQKV 240 REFP+AQ EAQAAFGS AVYLERFIR+ARHIEVQILGDGERAVHLFERECSLQRRRQKV Sbjct: 181 RREFPIAQREAQAAFGSPAVYLERFIRQARHIEVQILGDGERAVHLFERECSLQRRRQKV 240 Query: 241 LEEAPSPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEH 300 EEAPS AL AQRQALC+SAVRLA+ L YRGAGTLEYLFD SGEFFFIEMNTRIQVEH Sbjct: 241 FEEAPSAALDGAQRQALCESAVRLAQSLGYRGAGTLEYLFDAHSGEFFFIEMNTRIQVEH 300 Query: 301 PVTEMITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAE 360 PV+EM+TGIDLVQAMLRIA GE L +Q DIR++GAALEMRINAEDP RNFFP PGTV Sbjct: 301 PVSEMVTGIDLVQAMLRIAAGEALPWRQEDIRLNGAALEMRINAEDPARNFFPSPGTVER 360 Query: 361 LQWPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMAT 420 L+WPQGEG+RV+SHLY GYR+PPYYDSLLAKL+VHG+DR +A ARA+ A+ T + GMAT Sbjct: 361 LEWPQGEGVRVDSHLYPGYRVPPYYDSLLAKLIVHGRDRDEAFARARQALERTVLAGMAT 420 Query: 421 TLSLHQWLLADARVQSARFDTGALETWLAERT 452 TL LH+WLLADARVQ+A+FDT LETWLAERT Sbjct: 421 TLPLHRWLLADARVQAAQFDTATLETWLAERT 452 >NMB1861 [I] COG0439 Biotin carboxylase Nme Neisseria meningitidis MC58 Length = 453 Score = 474 bits (1219), Expect = e-133, Method: Compositional matrix adjust. Identities = 244/456 (53%), Positives = 322/456 (70%), Gaps = 8/456 (1%) Query: 5 LRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYL 64 L+K+LIANRGEIA+R++RA + +GI TVA S+AD DSL ++ADE IGPA + +SYL Sbjct: 2 LKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKLADESVCIGPAASAQSYL 61 Query: 65 NVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARR 124 NV A++ AA S ADA+HPGYGFL+ENA FAE V +G F+GP+ DTIR MGDK A+ Sbjct: 62 NVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTFIGPKPDTIRLMGDKVSAKH 121 Query: 125 TAAAAGVPVVPGSAGEL-EDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATELARE 183 AAGVP VPGS G L +D L A+++GYP++IKAS GGGGRG+R+ +L + Sbjct: 122 AMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKASGGGGGRGMRVVEKKEDLLQS 181 Query: 184 FPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLE 242 + +AEA AAFG+ VY+ER+++R RH+E+Q++ D A++L ER+CSLQRR QKV+E Sbjct: 182 VEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADEHGNAIYLAERDCSLQRRHQKVIE 241 Query: 243 EAPSPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPV 302 EAP+P +T +R + ++ +++ YRGAGT E+L++D GEFFFIEMNTR+QVEHPV Sbjct: 242 EAPAPFITEKERAKIGNACADACKRIGYRGAGTFEFLYED--GEFFFIEMNTRVQVEHPV 299 Query: 303 TEMITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAELQ 362 TE+ITG+D+VQ LRIA G PL +Q DI++ G A E RINAEDP NF P PG + Sbjct: 300 TELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHAFECRINAEDP-YNFIPSPGLIESCH 358 Query: 363 WPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATTL 422 P G GIRV+SH+Y GYRIPPYYDSL+ K+ VHGK R QA+A+ + A+ +TG+ T Sbjct: 359 LPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHGKTREQAMAKMRVALAELAVTGIKTNT 418 Query: 423 SLHQWLLADARVQSARFDTGALETWLAERTDTRAKQ 458 LH+ L ADA Q LE WL +R +AKQ Sbjct: 419 PLHRDLFADAGFQKGGVSIHYLEHWLEDR---KAKQ 451 >BMEI1063 [I] COG0439 Biotin carboxylase Bme Brucella melitensis Length = 459 Score = 470 bits (1210), Expect = e-132, Method: Compositional matrix adjust. Identities = 238/453 (52%), Positives = 313/453 (69%), Gaps = 4/453 (0%) Query: 5 LRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYL 64 +K+LIANRGEIA+R++RA + LGI TVA S AD D++ R+ADE IGP + SYL Sbjct: 9 FQKILIANRGEIALRVLRACKELGIKTVAVHSTADADAMHVRLADESVCIGPPPSRDSYL 68 Query: 65 NVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARR 124 N+ ++ A +GADA+HPGYGFLSENA FAE + A + F+GP A IR MGDK EA+R Sbjct: 69 NIHQIVAACEITGADAIHPGYGFLSENAKFAEILEAHDITFIGPTASHIRIMGDKIEAKR 128 Query: 125 TAAAAGVPVVPGSAGELEDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATELAREF 184 TA G+PVVPGS G + D A A+EIGYP++IKASAGGGGRG+++AR A EL Sbjct: 129 TAKRLGIPVVPGSDGGVTDEVEAARIAKEIGYPVIIKASAGGGGRGMKVARSAEELPIAL 188 Query: 185 PLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLEE 243 A++EA AAFG AVY+E+++ + RHIEVQ++GDG AVHL ER+CSLQRR QKV EE Sbjct: 189 ATARSEAGAAFGDDAVYIEKYLEKPRHIEVQVMGDGAGNAVHLGERDCSLQRRHQKVWEE 248 Query: 244 APSPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPVT 303 SPAL R+ + +L YRGAGT+E+L++D GEF+FIEMNTR+QVEHPVT Sbjct: 249 GNSPALNAEAREKIGMICANACAELGYRGAGTIEFLYED--GEFYFIEMNTRLQVEHPVT 306 Query: 304 EMITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAELQW 363 E ITGIDLV +R+A G L ++Q DIR G A+E RINAEDP F P PG + Sbjct: 307 EAITGIDLVHEQIRVAAGLGLSVKQKDIRFSGHAIECRINAEDP-LTFAPSPGLITHYHT 365 Query: 364 PQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATTLS 423 P G G+RV+S +Y+GYRIPPYYDSL KL+VHG++R + + R + A+ + G+ TTL Sbjct: 366 PGGLGVRVDSGVYSGYRIPPYYDSLTGKLIVHGRNRVECMMRLRRALDEFVVDGVKTTLP 425 Query: 424 LHQWLLADARVQSARFDTGALETWLAERTDTRA 456 L Q L+++ + + +D LE +LA ++ + Sbjct: 426 LFQDLISNQDIANGDYDIHWLEKYLANKSKNES 458 >NMA0596 [I] COG0439 Biotin carboxylase NmA Neisseria meningitidis Z2491 Length = 453 Score = 469 bits (1208), Expect = e-132, Method: Compositional matrix adjust. Identities = 245/456 (53%), Positives = 319/456 (69%), Gaps = 8/456 (1%) Query: 5 LRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYL 64 L+K+LIANRGEIA+R++RA + +GI TVA S+AD DSL ++ADE IGPA + +SYL Sbjct: 2 LKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKLADESVCIGPAASAQSYL 61 Query: 65 NVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARR 124 NV A++ AA S ADA+HPGYGFL+ENA FAE V +G F+GP+ DTIR MGDK A+ Sbjct: 62 NVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTFIGPKPDTIRLMGDKVSAKH 121 Query: 125 TAAAAGVPVVPGSAGEL-EDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATELARE 183 AAGVP VPGS G L +D L A++IGYP++IKAS GGGGRG+R+ +L + Sbjct: 122 AMIAAGVPCVPGSDGALPDDGEEILKIADKIGYPVIIKASGGGGGRGMRVVEKKEDLLQS 181 Query: 184 FPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLE 242 + +AEA AAFG+ VY+ER+++R RH+E+Q++ D AV+L ER+CSLQRR QKV+E Sbjct: 182 VEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADEHGNAVYLAERDCSLQRRHQKVIE 241 Query: 243 EAPSPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPV 302 EAP+P +T +R + + +++ YRGAGT E+L++D GEFFFIEMNTR+QVEHPV Sbjct: 242 EAPAPFITEKERAKIGKACADACKRIGYRGAGTFEFLYED--GEFFFIEMNTRVQVEHPV 299 Query: 303 TEMITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAELQ 362 TE+ITG+D+VQ LRIA G PL +Q DI++ G A E RINAEDP NF P PG + Sbjct: 300 TELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHAFECRINAEDP-YNFIPSPGPIESCH 358 Query: 363 WPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATTL 422 P G GIRV+SH+Y GYRIPPYYDSL+ K+ V GKDR QA+A+ + A+ ITG+ T Sbjct: 359 LPSGFGIRVDSHIYQGYRIPPYYDSLIGKVCVVGKDRDQAMAKMRVALAELAITGIKTNT 418 Query: 423 SLHQWLLADARVQSARFDTGALETWLAERTDTRAKQ 458 LH+ L D Q LE WL +R +AKQ Sbjct: 419 PLHRDLFNDPGFQKGGVSIHYLEHWLEDR---KAKQ 451 >AGc2451 [I] COG0439 Biotin carboxylase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 448 Score = 467 bits (1201), Expect = e-131, Method: Compositional matrix adjust. Identities = 241/446 (54%), Positives = 319/446 (71%), Gaps = 5/446 (1%) Query: 5 LRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYL 64 + K+LIANRGEIA+R++RAA+ LGI TVA S AD D++ R+ADE IGP + SYL Sbjct: 2 VSKILIANRGEIALRVLRAAKELGIPTVAVHSTADADAMHVRLADESVCIGPPPSRDSYL 61 Query: 65 NVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARR 124 N+ ++ A +GADA+HPGYGFLSENA FA+ ++A G+ F+GP A+ IR MGDK A++ Sbjct: 62 NIHQIVAACEITGADAVHPGYGFLSENAKFADILDAHGITFIGPTAEHIRIMGDKITAKQ 121 Query: 125 TAAAAGVPVVPGSAGELEDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATELAREF 184 TA G+PVVPGS GE++ AL A IG+P+LIKA+AGGGGRG+++A+ +L Sbjct: 122 TAQELGIPVVPGSDGEVKP-ENALEIARTIGFPVLIKATAGGGGRGMKVAKTEADLEDAV 180 Query: 185 PLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLEE 243 A++EA AAFG+ AVY+E+++ + RHIEVQ+ GDGE AVHL ER+CSLQRR QKVLEE Sbjct: 181 ATARSEAAAAFGNDAVYMEKYLGKPRHIEVQVFGDGEGNAVHLGERDCSLQRRHQKVLEE 240 Query: 244 APSPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPVT 303 A SPALT QR + + +L YRGAGT+E+L+ E+GEF+FIEMNTR+QVEHPVT Sbjct: 241 ANSPALTVEQRMKIGEICADAMRKLKYRGAGTIEFLY--ENGEFYFIEMNTRLQVEHPVT 298 Query: 304 EMITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAELQW 363 E ITG+DLVQ +R+A G+ L + Q+DI HG A+E RINAEDP R F P PGT+ Sbjct: 299 EAITGMDLVQEQIRVASGQGLSVTQADIEFHGHAIECRINAEDP-RTFVPSPGTLTYFHT 357 Query: 364 PQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATTLS 423 P G G+RV+S Y GY+IPPYYDS++ KL+VHG+DR + I R + A+ + G+ TTL Sbjct: 358 PGGLGVRVDSGAYQGYKIPPYYDSMIGKLIVHGRDRDECIRRLRRALDEFVVDGIKTTLP 417 Query: 424 LHQWLLADARVQSARFDTGALETWLA 449 L Q LL + + + +D LE +LA Sbjct: 418 LFQDLLQNEDILNGDYDIHWLEKYLA 443