BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0038.0212.p (663 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1286 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 426 e-119 PA3179 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase... 266 2e-70 STM1719 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 264 5e-70 yciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase a... 264 6e-70 ZyciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase ... 263 7e-70 >RSc1286 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Rso Ralstonia solanacearum Length = 598 Score = 426 bits (1096), Expect = e-119, Method: Compositional matrix adjust. Identities = 204/285 (71%), Positives = 232/285 (81%), Gaps = 7/285 (2%) Query: 255 DDIFSYVTSEAYDRDENPKGGARQPHQMRGKNGRRDLTAEDDAPKLHKVLADAGLGSRRD 314 +D+F YV S AYD D + GGA Q ++R +LTAEDDAPKLHK+LADAGLGSRRD Sbjct: 178 EDVFQYVISGAYDADAD-VGGAPQSTKVR------ELTAEDDAPKLHKILADAGLGSRRD 230 Query: 315 MEELIVAGRVSVNGEPAHIGQRILPTDAVRINGKLIQRKVSKRPPRVLVYHKPAGEIVSH 374 ME+LI+ GRVSVNG PAHIGQRILPTD VR+NGKLIQRK+ +PPRVL+YHKPAGEIVS Sbjct: 231 MEDLILQGRVSVNGLPAHIGQRILPTDQVRVNGKLIQRKLPSKPPRVLLYHKPAGEIVSQ 290 Query: 375 ADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTTSGDLANRLMHPRYNIEREYAV 434 +DPEGR +VFD LP MK GKW+AVGRLD+NTEGLL+FTTSGD+ANR MHPRY +EREYAV Sbjct: 291 SDPEGRPTVFDSLPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAV 350 Query: 435 RTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVNKWYRVTIGEGRNREVRRMFEA 494 RTLGEL E RQKLL G+ LDDG A F R ADGGGEGVN WY V + EGRNREVRRMFEA Sbjct: 351 RTLGELPESDRQKLLQGIRLDDGEANFLRCADGGGEGVNHWYHVALTEGRNREVRRMFEA 410 Query: 495 VGLTVSRLIRTRYGAMTLPQTLRRGRWEELEENAVRNLMAACGLE 539 V LTVSRLIRTRYG+ LP+ L+RGRW+E+ VR LM GL+ Sbjct: 411 VNLTVSRLIRTRYGSFVLPRGLKRGRWQEVSAEDVRGLMGLLGLK 455 >PA3179 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Pae Pseudomonas aeruginosa Length = 386 Score = 266 bits (679), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 132/251 (52%), Positives = 176/251 (70%), Gaps = 2/251 (0%) Query: 299 KLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRILPTDAVRINGKLIQRK-VSKR 357 KL KVLA G+GSRRD+E I GRV+VNG A +GQR+ DA+ ++G LI+R+ ++ Sbjct: 15 KLQKVLARLGVGSRRDVEVWIAEGRVNVNGSVASLGQRVDSHDAITVDGHLIRREEAAES 74 Query: 358 PPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTTSGDL 417 RVL+Y+KP GE+ + DPEGR ++FDRLP ++ G+W+ VGRLD NT GLLLFTT G+L Sbjct: 75 VRRVLIYNKPEGEVCTRDDPEGRPTIFDRLPRLRTGRWINVGRLDINTTGLLLFTTDGEL 134 Query: 418 ANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADG-GGEGVNKWY 476 ANRLMHP Y ++REYAVR GE+ E M ++LL+GV L+DGPA+FS I GGEG N WY Sbjct: 135 ANRLMHPSYEMDREYAVRVRGEVTEEMIERLLNGVMLEDGPAKFSDIQQAPGGEGFNHWY 194 Query: 477 RVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEELEENAVRNLMAAC 536 + EGRNREVRR++E+ GL VSRL R R+G + L L GR+ E+++ + L Sbjct: 195 HCVVMEGRNREVRRLWESQGLVVSRLKRVRFGPVFLTSELTMGRYREMDQREIDILSEEV 254 Query: 537 GLEKQAGEGKS 547 GL+ A G + Sbjct: 255 GLKPVALPGMT 265 >STM1719 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Sty Salmonella typhimurium LT2 Length = 291 Score = 264 bits (674), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 137/243 (56%), Positives = 176/243 (72%), Gaps = 6/243 (2%) Query: 297 APKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRI--LPTDAVRINGKLIQRKV 354 + KL KVLA AG GSRR++E +I AGRVSV+G+ A +G R+ P +RI+G LI K Sbjct: 2 SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIATLGDRVEVTPGLKIRIDGHLISVKE 61 Query: 355 S-KRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTT 413 S ++ RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT Sbjct: 62 SAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTT 121 Query: 414 SGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVN 473 G+LANRLMHP +EREYAVR G+++E + L GV+L+DGPA F I GGEG+N Sbjct: 122 DGELANRLMHPSREVEREYAVRVFGQVDESKLRDLSRGVQLEDGPAAFKTIKFSGGEGIN 181 Query: 474 KWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEEL---EENAVR 530 +WY VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL + N +R Sbjct: 182 QWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR 241 Query: 531 NLM 533 L+ Sbjct: 242 GLV 244 >yciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Eco Escherichia coli K12 Length = 291 Score = 264 bits (674), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 135/243 (55%), Positives = 177/243 (72%), Gaps = 6/243 (2%) Query: 297 APKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRI--LPTDAVRINGKLIQ-RK 353 + KL KVLA AG GSRR++E +I AGRVSV+G+ A +G R+ P +RI+G LI R+ Sbjct: 2 SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRE 61 Query: 354 VSKRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTT 413 +++ RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT Sbjct: 62 SAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTT 121 Query: 414 SGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVN 473 G+LANRLMHP +EREYAVR G++++ + L GV+L+DGPA F I GGEG+N Sbjct: 122 DGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKTIKFSGGEGIN 181 Query: 474 KWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEEL---EENAVR 530 +WY VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL + N +R Sbjct: 182 QWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR 241 Query: 531 NLM 533 L+ Sbjct: 242 ELV 244 >ZyciL [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases EcZ Escherichia coli O157:H7 EDL933 Length = 290 Score = 263 bits (673), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 135/243 (55%), Positives = 177/243 (72%), Gaps = 6/243 (2%) Query: 297 APKLHKVLADAGLGSRRDMEELIVAGRVSVNGEPAHIGQRI--LPTDAVRINGKLIQ-RK 353 + KL KVLA AG GSRR++E +I AGRVSV+G+ A +G R+ P +RI+G LI R+ Sbjct: 2 SEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKIRIDGHLISVRE 61 Query: 354 VSKRPPRVLVYHKPAGEIVSHADPEGRNSVFDRLPTMKAGKWLAVGRLDYNTEGLLLFTT 413 +++ RVL Y+KP GE+ + DPEGR +VFDRLP ++ +W+AVGRLD NT GLLLFTT Sbjct: 62 SAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGARWIAVGRLDVNTCGLLLFTT 121 Query: 414 SGDLANRLMHPRYNIEREYAVRTLGELEEGMRQKLLSGVELDDGPAQFSRIADGGGEGVN 473 G+LANRLMHP +EREYAVR G++++ + L GV+L+DGPA F I GGEG+N Sbjct: 122 DGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGVQLEDGPAAFKTIKFSGGEGIN 181 Query: 474 KWYRVTIGEGRNREVRRMFEAVGLTVSRLIRTRYGAMTLPQTLRRGRWEEL---EENAVR 530 +WY VT+ EGRNREVRR++EAVG+ VSRLIR RYG + LP+ L RG W EL + N +R Sbjct: 182 QWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLR 241 Query: 531 NLM 533 L+ Sbjct: 242 ELV 244