BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0032.p (234 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc02632 [R] COG1418 Predicted HD superfamily hydrolase Sme Sino... 268 1e-71 AGc1925 [R] COG1418 Predicted HD superfamily hydrolase Atu Agrob... 245 5e-65 yedJ [R] COG1418 Predicted HD superfamily hydrolase Eco Escheric... 171 1e-42 ZyedJ [R] COG1418 Predicted HD superfamily hydrolase EcZ Escheri... 171 2e-42 ECs2700 [R] COG1418 Predicted HD superfamily hydrolase Ecs Esche... 171 2e-42 >SMc02632 [R] COG1418 Predicted HD superfamily hydrolase Sme Sinorhizobium meliloti Length = 218 Score = 268 bits (684), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 133/212 (62%), Positives = 162/212 (76%), Gaps = 1/212 (0%) Query: 23 AAAFAPLDTLAADLLPHVMDDAGDGSHDLSHLLRVWNNACRLQKEEGGDVRILLAAVLLH 82 AA FAP LA +LLPH A DGSHD SHL+RVW NA R+Q EEGGD R L AAVLLH Sbjct: 8 AAPFAPHAELAGELLPHAFA-ADDGSHDASHLIRVWKNALRIQAEEGGDTRRLAAAVLLH 66 Query: 83 DCVRVEKNSPLRSQASAMSAQRAREILRDLRWDEAAIEAVAHAVQAHSYSAQVTPQSVEA 142 DCV VEKNSPLR++AS ++A++A IL L W A I +VAHA+ HS+SA + P+++EA Sbjct: 67 DCVSVEKNSPLRAEASRLAAEKASAILNGLGWSAADISSVAHAILTHSFSANLAPETLEA 126 Query: 143 KIVQDADRLDAIGMIGVARCFYVAGRMGSALYEPLDPQAQARPLEDGRYALDHFPAKLLR 202 +I+QDADRLDAIGM+G ARCFY+AGRM SALY+PLDP A++R L+D +A+DHF KL R Sbjct: 127 RILQDADRLDAIGMVGAARCFYIAGRMSSALYDPLDPLAESRALDDKAFAIDHFETKLFR 186 Query: 203 LAEGFQTACGARLAGERQARMRDFLTGFLAEL 234 LA+GFQTA G RLA ERQAR+R L L E+ Sbjct: 187 LADGFQTAAGRRLALERQARLRSVLAMLLDEI 218 >AGc1925 [R] COG1418 Predicted HD superfamily hydrolase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 214 Score = 245 bits (626), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 118/203 (58%), Positives = 153/203 (75%), Gaps = 1/203 (0%) Query: 32 LAADLLPHVMDDAGDGSHDLSHLLRVWNNACRLQKEEGGDVRILLAAVLLHDCVRVEKNS 91 LA DL+PH D GDGSHDL+H+ RV+ NA R+ EGGD +L A+VLLHDCV VEKNS Sbjct: 13 LAQDLIPHAAD-TGDGSHDLAHIHRVFRNAMRIHAGEGGDGNVLAASVLLHDCVAVEKNS 71 Query: 92 PLRSQASAMSAQRAREILRDLRWDEAAIEAVAHAVQAHSYSAQVTPQSVEAKIVQDADRL 151 PLR+QAS ++A++A IL L W + I+A AHA+ HS+SA + PQ++EAKI+QDADRL Sbjct: 72 PLRAQASRLAAEKASGILAGLGWQKQDIDAAAHAITTHSFSANIEPQTLEAKILQDADRL 131 Query: 152 DAIGMIGVARCFYVAGRMGSALYEPLDPQAQARPLEDGRYALDHFPAKLLRLAEGFQTAC 211 DAIGM+G ARCFY+AGRMGSALY+P DP A+ RPL+D +A+DHF KL +LA+GF+T Sbjct: 132 DAIGMVGAARCFYIAGRMGSALYDPADPLAKERPLDDRAFAIDHFETKLFKLADGFRTET 191 Query: 212 GARLAGERQARMRDFLTGFLAEL 234 G ++A R R++ L FL E+ Sbjct: 192 GRQMAKARHERLKQVLDLFLDEI 214 >yedJ [R] COG1418 Predicted HD superfamily hydrolase Eco Escherichia coli K12 Length = 231 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 89/191 (46%), Positives = 125/191 (65%), Gaps = 2/191 (1%) Query: 46 DGSHDLSHLLRVWNNACRLQKEEGGDVRILLAAVLLHDCVRVEKNSPLRSQASAMSAQRA 105 D +HD+ H RVW A +L ++ D+ ++L A HD V + KN P R ++S ++A+ Sbjct: 22 DAAHDVCHFRRVWATAQKLAADDDVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEET 81 Query: 106 REILRDL--RWDEAAIEAVAHAVQAHSYSAQVTPQSVEAKIVQDADRLDAIGMIGVARCF 163 R +LR+ ++ IEAV HA+ AHS+SAQ+ P + EAKIVQDADRL+A+G IG+AR F Sbjct: 82 RRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVF 141 Query: 164 YVAGRMGSALYEPLDPQAQARPLEDGRYALDHFPAKLLRLAEGFQTACGARLAGERQARM 223 V+G +G AL++ DP AQ RPL+D RYALDHF KLL+L + QTA G +LA + Sbjct: 142 AVSGALGVALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFL 201 Query: 224 RDFLTGFLAEL 234 +F+ AEL Sbjct: 202 VEFMAKLSAEL 212 >ZyedJ [R] COG1418 Predicted HD superfamily hydrolase EcZ Escherichia coli O157:H7 EDL933 Length = 231 Score = 171 bits (432), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 88/191 (46%), Positives = 124/191 (64%), Gaps = 2/191 (1%) Query: 46 DGSHDLSHLLRVWNNACRLQKEEGGDVRILLAAVLLHDCVRVEKNSPLRSQASAMSAQRA 105 D +HD+ H RVW A +L ++ D+ ++L A HD V + KN P R +S ++A+ Sbjct: 22 DAAHDVCHFRRVWATAQKLAADDDVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEET 81 Query: 106 REILRD--LRWDEAAIEAVAHAVQAHSYSAQVTPQSVEAKIVQDADRLDAIGMIGVARCF 163 R +LR+ +++ IEAV HA+ AHS+SAQ+ P + EAKIVQDADRL+A+G IG+AR F Sbjct: 82 RRLLREEFVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVF 141 Query: 164 YVAGRMGSALYEPLDPQAQARPLEDGRYALDHFPAKLLRLAEGFQTACGARLAGERQARM 223 V+G +G AL++ DP AQ RPL+D RYALDHF KLL+L + QT G +LA + Sbjct: 142 AVSGALGVALFDAEDPLAQHRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFL 201 Query: 224 RDFLTGFLAEL 234 +F+ AEL Sbjct: 202 VEFMAKLSAEL 212 >ECs2700 [R] COG1418 Predicted HD superfamily hydrolase Ecs Escherichia coli O157:H7 Length = 231 Score = 171 bits (432), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 88/191 (46%), Positives = 124/191 (64%), Gaps = 2/191 (1%) Query: 46 DGSHDLSHLLRVWNNACRLQKEEGGDVRILLAAVLLHDCVRVEKNSPLRSQASAMSAQRA 105 D +HD+ H RVW A +L ++ D+ ++L A HD V + KN P R +S ++A+ Sbjct: 22 DAAHDVCHFRRVWATAQKLAADDDVDMLVILTACYFHDIVSLAKNHPQRQSSSILAAEET 81 Query: 106 REILRD--LRWDEAAIEAVAHAVQAHSYSAQVTPQSVEAKIVQDADRLDAIGMIGVARCF 163 R +LR+ +++ IEAV HA+ AHS+SAQ+ P + EAKIVQDADRL+A+G IG+AR F Sbjct: 82 RRLLREEFVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVF 141 Query: 164 YVAGRMGSALYEPLDPQAQARPLEDGRYALDHFPAKLLRLAEGFQTACGARLAGERQARM 223 V+G +G AL++ DP AQ RPL+D RYALDHF KLL+L + QT G +LA + Sbjct: 142 AVSGALGVALFDAEDPLAQHRPLDDKRYALDHFQTKLLKLPQIMQTVRGKQLAQHNAHFL 201 Query: 224 RDFLTGFLAEL 234 +F+ AEL Sbjct: 202 VEFMAKLSAEL 212