BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0003.p (1925 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all5100 [R] COG2373 Large extracellular alpha-helical protein No... 158 1e-37 STM2532 [R] COG2373 Large extracellular alpha-helical protein St... 95 1e-18 yfhM [R] COG2373 Large extracellular alpha-helical protein Eco E... 88 2e-16 Z3787 [R] COG2373 Large extracellular alpha-helical protein EcZ ... 85 2e-15 ECs3386 [R] COG2373 Large extracellular alpha-helical protein Ec... 85 2e-15 >all5100 [R] COG2373 Large extracellular alpha-helical protein Nos Nostoc sp. PCC 7120 Length = 1906 Score = 158 bits (400), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 331/1519 (21%), Positives = 563/1519 (37%), Gaps = 206/1519 (13%) Query: 487 VTNLSVHVKLGRANGAVWVTRLDDAKPVADAEIRV--------SNCDGAQIWQGKTNASG 538 +TNL V + +G + V L D PV A I + S + GKT+ +G Sbjct: 507 LTNLGVFSQWFPESGLIRVNHLTDGAPVKAAVIEIYQSKLQAKSRPEPVPCATGKTDENG 566 Query: 539 VAMM--PALEDACANRGYSPDNADKINGLFVSARKTDEKGRQDMAFALTSWNEGIESWRF 596 + AL+ A N+ K L V A + ++D AF T G+ + Sbjct: 567 TFRVNREALQQCTAGS----QNSIKSPELLVIASE-----KEDWAFTRTEEYSGVYGYGI 617 Query: 597 NLPTDMDKSATVRATTVFDRTLFRAGETVSMKHVIRTETLQGFGLMRKEDLPTRVRISHL 656 + +K + R DR L+++GE + LQ G++ ++D +I+ + Sbjct: 618 DAGWQGNKPES-RGVIFSDRQLYQSGEKAWFTGF--ADYLQN-GVI-QQDKNADYQITLV 672 Query: 657 GSGQQYQFPLTWRGNKSAETTFVLPKQAKLG-SYEVVLDQGKVDANASAEPPEQSDDGDY 715 Q T N+ + +P G Y + +GK Sbjct: 673 NPDGQKTSLGTQTTNEFGTFSLEMPINKTQGLGYYTIQAKGK------------------ 714 Query: 716 EGGYGRGTYVTGSFRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYINGGAAANQAV 775 G ++G FRV EF+ P + + K ++ ++ NY+ G Sbjct: 715 -----NGLEISGEFRVAEFKPPNFKVEVKLDKEFAYIGDDVDINATSNYLFGAPVEGGEA 769 Query: 776 QVSSLLRSRSLNIAGYDGFSF--------DAPRSDEDSNADDQKIVADKLAITLDKNGAG 827 + + + G++ F+F +AP D + + LD N Sbjct: 770 KYFITRQQANFIPKGWEEFTFGRQWFWPEEAPTISSDVLQSNSQ---------LDGNDKS 820 Query: 828 RATISKLPALQRPQELLTEMTYSD-PNGEVQTISNVTPLWPSAVVVGLRTGNWVSVKKKL 886 ++ L P ++ +D N V + T L PS ++GL++ K Sbjct: 821 SQMVNVAKDLPYPMTYRVDVQVADVSNLSVANSQSFTAL-PSNRLIGLKSNFIADAGKAF 879 Query: 887 TLTAVTLNTAGQPQAGVPVEIRGTARKTNSHRKRMVGGFYAYENDETKQDLGKLCSGKSD 946 + + + G+ G V + K +S + + G E K + S+ Sbjct: 880 PIEVIIADPTGKLITGQRVRLELQQIKYSSVTQLVEGSQTPKNQVEYKTVAQTEITSTSN 939 Query: 947 QHGMFTCDTELSEAGNVELIASAKDGNGNAAAARSSVWVTGRGEVWFDGENQDRIDILPE 1006 + + E+G + + D A S +WVTG V++ ++D +++ + Sbjct: 940 PQ---SVNLTPPESGTYRIRVNFSDAKNELGATDSQIWVTGGNAVFWGTRDKDVLEVKLD 996 Query: 1007 KKNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQDPTISVPMKAEYGPNVY 1066 KK Y+ GETA ++ P+ A AV ++ I ++ ++ G P I + E PN Sbjct: 997 KKEYKAGETATALIQSPYADAELYFAVIKDKPIYQQITKVQGSAPQIQFRVTPEMLPNAA 1056 Query: 1067 VSVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIVDLSKPAWKFGIAEVNVG 1126 V + VR +PIN G + +L K F +VN+ Sbjct: 1057 VEAVLVRQ---------------GKPINQVEV-------GSLDNLVKIG--FTPFKVNLE 1092 Query: 1127 DAGHRLQV--AVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFALAAVDEALLELQPNG 1184 D +LQV A ++ +P A +Q++VK G P G + + V+EA+L+L Sbjct: 1093 DKYLKLQVKPAQASLEPG----AEETIQLEVKDNQGNPTKG-QLTVMVVNEAVLQLSGYR 1147 Query: 1185 SWDVLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGGGGRAP------TRELFDTL 1238 +++ + + + + V+ + K G GGG + TR F L Sbjct: 1148 PPNLVDTVYAEQPISTRFTDNRPDVILQPQDVAKPKGWGYGGGFSTGAANTRTRTDFQPL 1207 Query: 1239 LVWKPNVTLDAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTGSISIRSTQDVQVISGL 1298 + +V DA G AQ+ V L D LT++R++A+A +G FG G + +T+ + + L Sbjct: 1208 AYYNGSVLTDASGNAQITVKLPDDLTTWRVMAVATDGNLRFGNGDATFITTKPLLSNAIL 1267 Query: 1299 PPLVREGDSFNGAVTVRNTTTHDMTIKVAARAQGGTATSTLTLPEREVKIAAGQSAEVMW 1358 P VR GD ++V N T + + + G VK + Sbjct: 1268 PQFVRPGDRILAGLSVTNNTGNTGNLSINGEISGA------------VKFNSKNPTTTTL 1315 Query: 1359 PVQTPEGINQLAWEISAQEQGGQKAR---------DAMKFTQRVVPAVPVTVQQATLFQL 1409 Q + + A G K R DA + +V P + +T Q Sbjct: 1316 QTQAESATQAYRFPMVADSVGVGKVRFTTQLNGTADAFELPLQVKP-LEITEQIVETGVS 1374 Query: 1410 DKNAALDVARPADSLPGRGGLAISLKPSL-----AGGSDALT------------RYFVNY 1452 K + + ++ P GGL I L +L A LT + + Sbjct: 1375 QKQIKIPLNVDKNTFPEAGGLDIQLASTLIPEIKAPAKQVLTDNDLPFTEPSASQLIIAT 1434 Query: 1453 PFACLEQKTSKAIGLRDEAAWQKI-VADLPTYLDGDGLAYYFPPSDSGARRGSDTLTAYL 1511 + QK + + + V L DG FP G + ++AY Sbjct: 1435 NLQTIAQKYGQTFAEFNSRQQANLAVEKLQKLQISDGGFAAFP----GQEKSDPWVSAYS 1490 Query: 1512 LAATNEAGYAIPQASRDKMLDGLAAFVEGKVM-----RDFWSPQKDLDVRKLAALEALSR 1566 + + +A P ML L +++ KV+ DF Q +L AL ALS Sbjct: 1491 VESLVKASQVFPNLVDTGMLSRLKTYLQ-KVLANPGEYDFCKQQLCKRQLQLNALIALSE 1549 Query: 1567 YNRVQPAMLGSLQINPNVWPTSGLLDWIAILQRVPAIPDRQKKLDEADQIL-RARLNFQG 1625 + L + N + L+ I + + + P+ Q DE+ Q++ + + N Sbjct: 1550 LGDKRNTFLTDIYEQRNNF---DLVTQIKLARYLSQFPEWQ---DESQQLVNKLQQNISE 1603 Query: 1626 TRMGFSNEEGDYWWWLMS--SGDANANRLILLELNNPAWREDMPRLIAGAIGRQQRGHWS 1683 T W W+ S + A A RL + + + P E + +L+ + ++ G W Sbjct: 1604 TGRTAVVSLPPSWGWMSSPTTAQAQALRLFIAKQSKP---EIIDKLLQSLLALRRDGTWQ 1660 Query: 1684 TTTANVWGSLALEKFARKFESEPVTGATRATLEQNGASAATQSLAWSGAPNGGKLQLPWP 1743 T N AL ++ + +P AT++ G P+ LQL P Sbjct: 1661 TDYNNAQALTALVEYG---QLQPTPPNFVATVQLAGRKLGENRFTGYKNPS---LQLSVP 1714 Query: 1744 EGNAQGG--SVRLAQEGNGKPWVTLQSLAAVPLK----QP-FAAGYRITRKVEAIEQK-- 1794 G + L + GNG TL L A + QP G RITR++ + K Sbjct: 1715 MNQLPRGRHDLTLQKSGNG----TLHYLVAYNYRLQGNQPGRFNGLRITREISQVNAKRI 1770 Query: 1795 --QAGVYTR--------GDVLRVTLDIDAQAEMTWVVVSDPVPGGATLLGSGLGRDSAIA 1844 + G+Y G V + L+I + +V+ DP+P G + + +A Sbjct: 1771 LRKTGIYALDQPLTLALGQVFDIGLEIIVDRPVDHLVIKDPLPAGLEAVDASFQTTTAAL 1830 Query: 1845 QGKQDAPRERGMGW-LAYEERSFEAYRAYYEAMPKGRTSISYTVRLNNAGEFNLPPTRVE 1903 Q K D+ W L + + AY + + G S+ Y VR G F+ P V Sbjct: 1831 QAKADS-------WELGFRNIYSDRIIAYADHLEPGVYSLHYLVRSVTPGTFSWPGAEVH 1883 Query: 1904 AMYAPEMFGEVPNGKLVVK 1922 YAPE FG KL+V+ Sbjct: 1884 LQYAPEEFGRTAEMKLIVE 1902 >STM2532 [R] COG2373 Large extracellular alpha-helical protein Sty Salmonella typhimurium LT2 Length = 1644 Score = 95.1 bits (235), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 267/1270 (21%), Positives = 454/1270 (35%), Gaps = 198/1270 (15%) Query: 729 FRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYINGGAAANQAVQVSSLLRSRSLNI 788 F VE+F M +T K E+ + Y+ G A +Q LR + Sbjct: 469 FHVEDFMPERMALNLTAQKTPLAPADEVKFSVVGYYLYGAPANGNTLQGQLFLRPLRDAV 528 Query: 789 AGYDGFSFDAPRSDEDSNADDQKIVADKLAITLDKNGAGRATI-SKLPALQRPQELLTEM 847 A GF F + S + D++ +TLDK G G + S+ P +++ + Sbjct: 529 AALPGFQFGNIAEENLSRS------LDEVQLTLDKGGRGEVSAASQWQEAHSPLQVILQA 582 Query: 848 TYSDPNGEVQTISNVTPLWPSAVVVGLR---------------TGNW--VSVKKKLTLTA 890 + + G T +WP+ + G+R T N V Sbjct: 583 SLLESGGRPVTRRVEQAIWPADTLPGIRPQFAAKAVYDYRTDTTVNQPIVDEDSNAAFDI 642 Query: 891 VTLNTAGQPQAGVPVEIRGTARKTNSHRKRMVGGFYAYENDETKQDLGKLCSGKSDQHGM 950 V N G+ +A + G + R+ + E +++ D L G+ Sbjct: 643 VYANAQGEKKA-----VSGLQVRLIRERRDYYWNWSESEGWQSQFDQKDLVEGEQ----- 692 Query: 951 FTCDTELSEAGNVELIAS-------AKDGNGNAAAARSSVWVTGRGEVWFDGEN------ 997 T D E G V K N ++ R W G W D + Sbjct: 693 -TLDLNADETGKVSFPVEWGAYRLEVKAPNETVSSVR--FWA---GYSWQDNSDGSGAAR 746 Query: 998 QDRIDILPEKKNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQDPTISVPM 1057 DR+ + +K NY+PG+T + P + +G + + + + Q +++P+ Sbjct: 747 PDRVTLKLDKANYRPGDTMKLHIAAPVAGKGYAMVESSDGPLWWQAIDVPAQGLELTIPV 806 Query: 1058 KAEYG-PNVYVSVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIVDLSKPAW 1116 + ++Y+S L VR PG + P Sbjct: 807 DKTWNRHDLYLSTLVVR-------------------------------PGDKSRSATPKR 835 Query: 1117 KFGIAEVNVGDAGHRLQVAVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFALAAVDEA 1176 G+ + +GD RL +A+ + P + + V+V+ + +G+ ++AVD Sbjct: 836 AVGLLHLPLGDDNRRLDLALESPAKMRPNQPLT-VRVKASVKHGEMPKQINVLVSAVDSG 894 Query: 1177 LLELQPNGSWDVLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGG-------GGRA 1229 +L + + D A ++ YG + QV+ + G+ A GG GG+ Sbjct: 895 VLNITDYATPDPWQAFFGQKRYGADIYDIYGQVIEGQ--GRLAALRFGGDGDDLKRGGKP 952 Query: 1230 PTRELFDTLLVWKPNVTLDAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTGSISIRST 1289 P + +P +TL+ +G + +P+ D R++A A DFG G + Sbjct: 953 PVNHVNIIAQQAQP-ITLNEQGEGVVTLPIGDFNGELRVMAQAWTA-DDFGRGESKVVVA 1010 Query: 1290 QDVQVISGLPPLVREGDSFNGAVTVRNTTTHDMTIKVAARAQGGTATSTLTLPEREVKIA 1349 V +P + GD + V N T T+ +A A G L+ + V +A Sbjct: 1011 APVIAELNMPRFLAGGDVSRLVLDVTNLTDRPQTLNIALAASG--LLELLSQQPQPVNLA 1068 Query: 1350 AGQSAEVMWPVQTPEGINQLAWEISAQEQGGQKARDAMKFTQR-----VVPAVPV-TVQQ 1403 G + PV+ EG + EI A G + + + V PA P TV Sbjct: 1069 PGVRTTLFVPVRALEGFGE--GEIQATISGLNLPGETLDAQHKQWQIGVRPAWPAQTVNS 1126 Query: 1404 ATLFQLDKNAALDVARPADSLPG--RGGLAISLKPSLAGGSDALTRYFVN---YPFACLE 1458 ++ + A+ P +G L +S KP L L RY YP+ CLE Sbjct: 1127 GIALAPGESWHVPEQHLANISPATLQGQLLLSGKPPL-----NLARYIRELKAYPYGCLE 1181 Query: 1459 QKTS-------------KAIGLRDEAAWQK-------IVADLPTYLDGDGLAYYFPPSDS 1498 Q TS +++G+ ++ ++ I L D G A + ++ Sbjct: 1182 QTTSGLFPALYTNAAQLQSLGITGDSDEKRRAAVDIGISRILQMQRDNGGFALW---DEN 1238 Query: 1499 GARRGSDTLTAY---LLAATNEAGYAIPQASRDKMLDGLAAFVEGK-VMRDFWSPQKDLD 1554 GA LTAY L E GY++P + ++ + L +++ M +S Sbjct: 1239 GAEE--PWLTAYAMDFLIRAGEQGYSVPPEAINRGNERLLRYLQDPGTMLIRYSDNTQAS 1296 Query: 1555 VRKLAALEALSRYNRVQPAMLGSLQINPNVWPTSGLLDWIAILQRVPAIPDRQKKLDEAD 1614 A AL R Q A LG+L+ +W Q +P Q + + Sbjct: 1297 TFAAQAYAALV-LARQQKAPLGTLR---EIWERRS--------QAASGLPLMQLGI-ALN 1343 Query: 1615 QILRARLNFQGTRMGFSNEEGDYWWWLMSSGDA---NANRLILLELNNPAWREDMPRLIA 1671 + AR + + + D W+ G + NA L LLE NN R D + Sbjct: 1344 TMGDARRGEEAITLALNTPRQDERQWIADYGSSLRDNALMLSLLEENN--LRPDAQNALL 1401 Query: 1672 GAIGRQQRGH-WSTTTANVWGSLALEKFARKFESEPVTGATRATLEQNGASAATQSLAWS 1730 ++ Q G W +T N +L L +R Q A A + Sbjct: 1402 SSLSEQAFGQRWLSTQEN--NALFLAAHSR----------------QASAGAWQVQTSLE 1443 Query: 1731 GAPNGGKLQLPWPEGNAQGGSVRLAQEGNGKPWVTLQSLAAVPLKQPFAAG--YRITRKV 1788 P G L Q ++ + G+ W+ L S + P P A +I R++ Sbjct: 1444 AQPLSGDKALTRNLDADQLAALEVTNTGSQPLWLRLDS-SGYPSSAPEPASNVLQIERQI 1502 Query: 1789 EAI--EQKQAGVYTRGDVLRVTLDIDAQAEMTWVVVSDPVPGGATLLGSGLGRDSAI--- 1843 ++K G+++ V L + A + +V D +P G L L SA Sbjct: 1503 LGTDGQRKSLSSLRSGELVLVWLTVVADRNVPDALVVDLLPAGLELENQNLADSSASLPE 1562 Query: 1844 ----AQGKQDAPRERGMGWLAYEERSFEAYRAYYEAMPKGRTSISYTVRLNNAGEFNLPP 1899 Q + ++ + ++ + + F A E P ++ Y R G + L Sbjct: 1563 SGSEVQNLLNQMQQADIQYMEFRDDRFVAAVVVNEGQP---VTLVYLARAVTPGTYQLAQ 1619 Query: 1900 TRVEAMYAPE 1909 +VE+MYAP+ Sbjct: 1620 PQVESMYAPQ 1629 >yfhM [R] COG2373 Large extracellular alpha-helical protein Eco Escherichia coli K12 Length = 1653 Score = 88.2 bits (217), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 170/793 (21%), Positives = 288/793 (36%), Gaps = 117/793 (14%) Query: 720 GRGTYVTGSFRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYINGGAAANQAVQVSS 779 G Y F VE+F M +T K E+ + Y+ G A +Q Sbjct: 468 GDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSVVGYYLYGAPANGNTLQGQL 527 Query: 780 LLRSRSLNIAGYDGFSFDAPRSDEDSNADDQKIVADKLAITLDKNGAGR-ATISKLPALQ 838 LR ++ GF F D A++ D++ +TLD G G +T S+ Sbjct: 528 FLRPLREAVSALPGFEFG------DIAAENLSRTLDEVQLTLDDKGRGEVSTESQWKETH 581 Query: 839 RPQELLTEMTYSDPNGEVQTISNVTPLWPSAVVVGLRTGNWVSVKKKLTLTAVTLNTAGQ 898 P +++ + + + G T +WP+ + G+R K T +T Q Sbjct: 582 SPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRP----QFASKSVYDYRTDSTVKQ 637 Query: 899 PQAG---------VPVEIRGTARKTNSHRKRMV----GGFYAYENDETKQDLGKLCSGKS 945 P V + +G + + + R++ ++ + DE Q + Sbjct: 638 PIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSEDEGWQ-------SQF 690 Query: 946 DQHGMF----TCDTELSEAGNVELIAS-----AKDGNGNAAAARSSVWVTGRGEVWFDGE 996 DQ + T D + E G V + N A + W G W D Sbjct: 691 DQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWA---GYSWQDNS 747 Query: 997 N------QDRIDILPEKKNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQD 1050 + DR+ + +K +Y+PG+T + P + EG + + + + Q Sbjct: 748 DGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWWQEIDVRAQG 807 Query: 1051 PTISVPMKAEYG-PNVYVSVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIV 1109 +++P+ + ++Y+S L VR PG Sbjct: 808 LDLTIPVDKTWNRHDLYLSTLVVR-------------------------------PGDKS 836 Query: 1110 DLSKPAWKFGIAEVNVGDAGHRLQVAVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFA 1169 + P G+ + +GD RL +A+ T P + + V+++ NG+ Sbjct: 837 RSATPKRAVGVLHLPLGDENRRLDLALETPAKMRPNQPLT-VKIKASTKNGEKPKQVNVL 895 Query: 1170 LAAVDEALLELQPNGSWDVLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGG---- 1225 ++AVD +L + + D A ++ YG + QV+ + G+ A GG Sbjct: 896 VSAVDSGVLNITDYVTPDPWQAFFGQKRYGADIYDIYGQVIEGQ--GRLAALRFGGDGDE 953 Query: 1226 ---GGRAPTRELFDTLLVWKPNVTLDAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTG 1282 GG+ P + + P VTL+ +G + +P+ D R++A A DFG+ Sbjct: 954 LKRGGKPPVNHVNIVVQQALP-VTLNEQGEGSVTLPIGDFNGELRVMAQAWTA-DDFGSN 1011 Query: 1283 SISIRSTQDVQVISGLPPLVREGDSFNGAVTVRNTTTHDMTIKVAARAQGGTATSTLTLP 1342 + V +P + GD+ + + N T + VA A G ++ Sbjct: 1012 ESKVIVAAPVIAELNMPRFMASGDTSRLTLDITNLTDKPQKLNVALTASG--LLELVSDS 1069 Query: 1343 EREVKIAAGQSAEVMWPVQTPEGI--NQLAWEISAQEQGGQKARDAMK-FTQRVVPAVPV 1399 V++A G + PV+ G ++ IS G+ D K + V PA P Sbjct: 1070 PAAVELAPGVRTTLFIPVRALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPA 1129 Query: 1400 -TVQQATLFQLDKNAALDVARPADSLPG------RGGLAISLKPSLAGGSDALTRYFVN- 1451 TV T Q + A+ PAD L G L +S KP L + RY Sbjct: 1130 QTVNYGTALQPGETWAI----PADGLQNFSPVTLEGQLLLSGKPPL-----NIARYIKEL 1180 Query: 1452 --YPFACLEQKTS 1462 YP+ CLEQ S Sbjct: 1181 KAYPYGCLEQTAS 1193 >Z3787 [R] COG2373 Large extracellular alpha-helical protein EcZ Escherichia coli O157:H7 EDL933 Length = 1653 Score = 84.7 bits (208), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 169/793 (21%), Positives = 286/793 (36%), Gaps = 117/793 (14%) Query: 720 GRGTYVTGSFRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYINGGAAANQAVQVSS 779 G Y F VE+F M +T K E+ + Y+ G A +Q Sbjct: 468 GDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSVVGYYLYGAPANGNTLQGQL 527 Query: 780 LLRSRSLNIAGYDGFSFDAPRSDEDSNADDQKIVADKLAITLDKNGAGR-ATISKLPALQ 838 LR ++ GF F D A++ D++ +TLD G G +T S+ Sbjct: 528 FLRPLREAVSALPGFEFG------DIAAENLSRTLDEVQLTLDDKGRGEVSTESQWKETH 581 Query: 839 RPQELLTEMTYSDPNGEVQTISNVTPLWPSAVVVGLRTGNWVSVKKKLTLTAVTLNTAGQ 898 P +++ + + + G T +WP+ + G+R K T +T Q Sbjct: 582 SPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRP----QFASKSVYDYRTDSTVKQ 637 Query: 899 PQAG---------VPVEIRGTARKTNSHRKRMV----GGFYAYENDETKQDLGKLCSGKS 945 P V + +G + + + R++ ++ + DE Q + Sbjct: 638 PIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSEDEGWQ-------SQF 690 Query: 946 DQHGMF----TCDTELSEAGNVELIAS-----AKDGNGNAAAARSSVWVTGRGEVWFDGE 996 DQ + T D + E G V + N A + W G W D Sbjct: 691 DQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWA---GYSWQDNS 747 Query: 997 N------QDRIDILPEKKNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQD 1050 + DR+ + +K Y+PG+T + P + EG + + + + Q Sbjct: 748 DGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWWQEIDVPAQG 807 Query: 1051 PTISVPMKAEYG-PNVYVSVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIV 1109 +++P+ + ++Y+S L VR PG Sbjct: 808 LDLTIPVDKTWNRHDLYLSTLVVR-------------------------------PGDKS 836 Query: 1110 DLSKPAWKFGIAEVNVGDAGHRLQVAVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFA 1169 + P G+ + +GD RL +A+ T P + + V+++ NG+ Sbjct: 837 RSATPKRAVGVLHLPLGDENRRLDLALETPAKMRPNQPLT-VKIKASTKNGEKPKQVNVL 895 Query: 1170 LAAVDEALLELQPNGSWDVLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGG---- 1225 ++AVD +L + + D A ++ YG + QV+ + G+ A GG Sbjct: 896 VSAVDSGVLNITDYVTPDPWQAFFGQKRYGADIYDIYGQVIEGQ--GRLAALRFGGDGDE 953 Query: 1226 ---GGRAPTRELFDTLLVWKPNVTLDAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTG 1282 GG+ P + P VTL+ +G + +P+ D R++A A +FG+ Sbjct: 954 LKRGGKPPVNHVNIVAQQALP-VTLNEQGEGSVTLPIGDFNGELRVMAQAWTA-DEFGSN 1011 Query: 1283 SISIRSTQDVQVISGLPPLVREGDSFNGAVTVRNTTTHDMTIKVAARAQGGTATSTLTLP 1342 + V +P + GD+ + + N T + VA A G ++ Sbjct: 1012 ESKVIVAAPVIAELNMPRFMASGDNSRLTLDITNLTDKPQKLNVALTASG--LLELVSDS 1069 Query: 1343 EREVKIAAGQSAEVMWPVQTPEGI--NQLAWEISAQEQGGQKARDAMK-FTQRVVPAVPV 1399 V++A G + PV+ G ++ IS G+ D K + V PA P Sbjct: 1070 PAAVELAPGVRTTLFIPVRALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPA 1129 Query: 1400 -TVQQATLFQLDKNAALDVARPADSLPG------RGGLAISLKPSLAGGSDALTRYFVN- 1451 TV T Q + A+ PAD L G L +S KP L + RY Sbjct: 1130 QTVNYGTALQPGETWAI----PADGLQNFSPVTLEGQLLLSGKPPL-----NIARYIKEL 1180 Query: 1452 --YPFACLEQKTS 1462 YP+ CLEQ S Sbjct: 1181 KAYPYGCLEQTAS 1193 >ECs3386 [R] COG2373 Large extracellular alpha-helical protein Ecs Escherichia coli O157:H7 Length = 1653 Score = 84.7 bits (208), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 169/793 (21%), Positives = 286/793 (36%), Gaps = 117/793 (14%) Query: 720 GRGTYVTGSFRVEEFRLPLMQGRITPPKGAQVAVKEMPLDLQLNYINGGAAANQAVQVSS 779 G Y F VE+F M +T K E+ + Y+ G A +Q Sbjct: 468 GDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSVVGYYLYGAPANGNTLQGQL 527 Query: 780 LLRSRSLNIAGYDGFSFDAPRSDEDSNADDQKIVADKLAITLDKNGAGR-ATISKLPALQ 838 LR ++ GF F D A++ D++ +TLD G G +T S+ Sbjct: 528 FLRPLREAVSALPGFEFG------DIAAENLSRTLDEVQLTLDDKGRGEVSTESQWKETH 581 Query: 839 RPQELLTEMTYSDPNGEVQTISNVTPLWPSAVVVGLRTGNWVSVKKKLTLTAVTLNTAGQ 898 P +++ + + + G T +WP+ + G+R K T +T Q Sbjct: 582 SPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRP----QFASKSVYDYRTDSTVKQ 637 Query: 899 PQAG---------VPVEIRGTARKTNSHRKRMV----GGFYAYENDETKQDLGKLCSGKS 945 P V + +G + + + R++ ++ + DE Q + Sbjct: 638 PIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSEDEGWQ-------SQF 690 Query: 946 DQHGMF----TCDTELSEAGNVELIAS-----AKDGNGNAAAARSSVWVTGRGEVWFDGE 996 DQ + T D + E G V + N A + W G W D Sbjct: 691 DQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWA---GYSWQDNS 747 Query: 997 N------QDRIDILPEKKNYQPGETANFQVRMPFRSATALVAVEREGVIDTRVVQLSGQD 1050 + DR+ + +K Y+PG+T + P + EG + + + + Q Sbjct: 748 DGSGAVRPDRVTLKLDKAGYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWWQEIDVPAQG 807 Query: 1051 PTISVPMKAEYG-PNVYVSVLAVRGRMREVPWYSFFIWGWKEPINWWHEFREYQGPGPIV 1109 +++P+ + ++Y+S L VR PG Sbjct: 808 LDLTIPVDKTWNRHDLYLSTLVVR-------------------------------PGDKS 836 Query: 1110 DLSKPAWKFGIAEVNVGDAGHRLQVAVSTDKPTYPIRAIAKVQVQVKLPNGKPAAGGEFA 1169 + P G+ + +GD RL +A+ T P + + V+++ NG+ Sbjct: 837 RSATPKRAVGVLHLPLGDENRRLDLALETPAKMRPNQPLT-VKIKASTKNGEKPKQVNVL 895 Query: 1170 LAAVDEALLELQPNGSWDVLHAMLQRRSYGVETSTAQMQVVGKRHYGKKATPAGGG---- 1225 ++AVD +L + + D A ++ YG + QV+ + G+ A GG Sbjct: 896 VSAVDSGVLNITDYVTPDPWQAFFGQKRYGADIYDIYGQVIEGQ--GRLAALRFGGDGDE 953 Query: 1226 ---GGRAPTRELFDTLLVWKPNVTLDAEGRAQLDVPLNDALTSFRIVAIAENGISDFGTG 1282 GG+ P + P VTL+ +G + +P+ D R++A A +FG+ Sbjct: 954 LKRGGKPPVNHVNIVAQQALP-VTLNEQGEGSVTLPIGDFNGELRVMAQAWTA-DEFGSN 1011 Query: 1283 SISIRSTQDVQVISGLPPLVREGDSFNGAVTVRNTTTHDMTIKVAARAQGGTATSTLTLP 1342 + V +P + GD+ + + N T + VA A G ++ Sbjct: 1012 ESKVIVAAPVIAELNMPRFMASGDNSRLTLDITNLTDKPQKLNVALTASG--LLELVSDS 1069 Query: 1343 EREVKIAAGQSAEVMWPVQTPEGI--NQLAWEISAQEQGGQKARDAMK-FTQRVVPAVPV 1399 V++A G + PV+ G ++ IS G+ D K + V PA P Sbjct: 1070 PAAVELAPGVRTTLFIPVRALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPA 1129 Query: 1400 -TVQQATLFQLDKNAALDVARPADSLPG------RGGLAISLKPSLAGGSDALTRYFVN- 1451 TV T Q + A+ PAD L G L +S KP L + RY Sbjct: 1130 QTVNYGTALQPGETWAI----PADGLQNFSPVTLEGQLLLSGKPPL-----NIARYIKEL 1180 Query: 1452 --YPFACLEQKTS 1462 YP+ CLEQ S Sbjct: 1181 KAYPYGCLEQTAS 1193