BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0213.p (269 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1248 [R] COG4188 Predicted dienelactone hydrolase Rso Ralston... 311 9e-85 AGc423 [R] COG4188 Predicted dienelactone hydrolase Atu Agrobact... 128 2e-29 CC3039 Non COG 117 2e-26 AGc4418 [R] COG4188 Predicted dienelactone hydrolase Atu Agrobac... 97 3e-20 all0969_2 [R] COG4188 Predicted dienelactone hydrolase Nos Nosto... 93 8e-19 >RSp1248 [R] COG4188 Predicted dienelactone hydrolase Rso Ralstonia solanacearum Length = 372 Score = 311 bits (797), Expect = 9e-85, Method: Compositional matrix adjust. Identities = 163/266 (61%), Positives = 194/266 (72%), Gaps = 6/266 (2%) Query: 1 VVFSHGTGGSADELDWLASAFAKHGYIVAGVNHPGNNALEPLTREGFVLWWERATDLSEV 60 ++ SHGTGG+AD LDWLA+A A GYIVAGV+HPGN+A PLTREGF LWWERATDLS+V Sbjct: 103 LLLSHGTGGTADSLDWLAAALAAQGYIVAGVDHPGNSAQAPLTREGFRLWWERATDLSQV 162 Query: 61 LDAMLADKAFGPHIDPRRVGAAGFSLGGYTVLELAGARTDLYAFRAYCHSPAADSNCHPP 120 LD +LAD A GP ID R+GA GFSLGGYTVLELAGART+L AF +C SP AD+ CHPP Sbjct: 163 LDGLLADPALGPRIDADRIGAVGFSLGGYTVLELAGARTNLPAFERFCASPDADAICHPP 222 Query: 121 -----TNYASRKVAPMSASAIASMKRSGASYRDLRIKAVFAMAPAIGKALDQQSLTRIEI 175 + A AP +A AS+ R+GASYRD RI+AVFA+APA+G A D + + I Sbjct: 223 EMRNVQDDAQPAGAPSPETA-ASLARAGASYRDPRIRAVFAIAPALGMAFDDTAFAEVRI 281 Query: 176 PVRLIAGTADTTAPPTTNVLHIAQFLPRARVRMIDGATHYTFLDTCVPAAMTIHATICKE 235 PV LIAG+AD TAP TN+ I + LP A ++ GATHYTFLDTC+P + A ICK+ Sbjct: 282 PVALIAGSADITAPVETNIRRIGKLLPGASTELVPGATHYTFLDTCLPPLVERLAPICKD 341 Query: 236 APGVDRDAVHAIAIGEAFDFFDSSLA 261 APGVDRDAVHA I A FF +SL+ Sbjct: 342 APGVDRDAVHAQTITRALAFFAASLS 367 >AGc423 [R] COG4188 Predicted dienelactone hydrolase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 395 Score = 128 bits (321), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 81/257 (31%), Positives = 121/257 (47%), Gaps = 12/257 (4%) Query: 1 VVFSHGTGGSADELDWLASAFAKHGYIVAGVNHPGNNALEPLTREGFVLWWERATDLSEV 60 V+ SHG+G + W+A A G+IVAG NHPG + + + +W ER +DLS + Sbjct: 122 VLLSHGSGSRVSGMAWIAEKLASEGFIVAGTNHPGTTSGDSTPADTPKIW-ERTSDLSAI 180 Query: 61 LDAMLADKAFGPHIDPRRVGAAGFSLGGYTVLELAGARTDLYAFRAYCHSPAADSNCHP- 119 + A+ + ID R+G GFSLGG +E++GAR DL A+ YC AA +C Sbjct: 181 VTALTTQGKWSASIDAGRIGVLGFSLGGSAAMEISGARADLEAYARYCEKEAAMMDCQWF 240 Query: 120 PTNYASRKVAPMSASAIASMKRSGASY----RDLRIKAVFAMAPAIGKALDQQSLTRIEI 175 A P+S + A + RD RI++ + P + A +SL +I+I Sbjct: 241 DGGQAYVNNEPVSVPKLDLRTLDKARFEQQNRDPRIRSAVLVDPGLALAFQPESLRKIDI 300 Query: 176 PVRLIAGTADTTAPPTTNVLHIAQFLPRARVRMIDGATHYTFLDTCVPAAMTIHAT---- 231 P+ I + PP +A +P A R +D A H++FL C A + Sbjct: 301 PLTFINLGSKGKIPPAVLADRLAAEVPGATYRQVDEADHFSFLPLCKEGASAFLKSVGER 360 Query: 232 --ICKEAPGVDRDAVHA 246 IC+ DR +HA Sbjct: 361 DPICEPTGPRDRSDIHA 377 >CC3039 Non COG Length = 335 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 88/270 (32%), Positives = 117/270 (43%), Gaps = 15/270 (5%) Query: 1 VVFSHGTGGSADELDWLASAFAKHGYIVAGVNHPGNNALEPLTREGFVLWWERATDLSEV 60 +V SHG GGS A A A+ G++VA + HPG+N + +R LS + Sbjct: 79 IVMSHGNGGSFAGHADTAQALAEAGFVVAALTHPGDNYKDQSRATAMA---DRPAALSSL 135 Query: 61 LDAMLADKAFGPHIDPRRVGAAGFSLGGYTVLELAGARTDLYAFRAYCHSPAADSNCHPP 120 + ML +DP +VGA GFS GG+TVL AG DL +C A+ +C Sbjct: 136 IGWMLEASPLKAKLDPAKVGAFGFSSGGFTVLAAAGGEPDLSRMLGHCLKHPANFDCKLT 195 Query: 121 TNYASRKVAPMSASAIASMKRSGASYRDLRIKAVFAMAPAIGKALDQQSLTRIEIPVRLI 180 N P A+ +A+ D RIKAV A APA+G ++ L I PV+L Sbjct: 196 ANR------PAPANVLAAR-----WTHDPRIKAVVAAAPALGFTFGKRGLKGITAPVQLW 244 Query: 181 AGTADTTAPPTTNVLHIAQFLPRAR-VRMIDGATHYTFLDTCVPAAMTIHATICKEAPGV 239 D P + + L R R++ GA H+ FL C P +C APG Sbjct: 245 KAGDDQILPGDDYAEAVHRNLKRPHDYRVVPGAGHFDFLAPCAPTPPAELVYLCASAPGF 304 Query: 240 DRDAVHAIAIGEAFDFFDSSLAVPITEKSK 269 DR A H E FF L P K Sbjct: 305 DRKAFHQTFNTEVVRFFTHQLVAPAKTAQK 334 >AGc4418 [R] COG4188 Predicted dienelactone hydrolase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 248 Score = 97.4 bits (241), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 71/237 (29%), Positives = 107/237 (45%), Gaps = 16/237 (6%) Query: 21 FAKHGYIVAGVNHPGNNALEPLTREGFVLWWERATDLSEVLDAMLADKAFGPHIDPRRVG 80 + GY+VA +H G + E E V WER D+S L A+L ID +R+ Sbjct: 2 LVQQGYVVAAPDHNGESFTEANATE-IVPLWERPRDISRTLTALLDRDDLAGRIDSQRIA 60 Query: 81 AAGFSLGGYTVLELAGARTDLYAFRAYCHSPAADSNCHPPTNYASRKVAPMSASAIASMK 140 G SLGG+T +ELAGAR C++ C P + Sbjct: 61 VIGHSLGGWTAMELAGARYSADLALKDCNTEKLPPQCKAPRLLGKV----GIVGGGKADP 116 Query: 141 RSGASYRDLRIKAVFAMAPAIGKALDQQSLTRIEIPVRLIAGTADT--TAPPTTNVLHIA 198 R +RD RI+AV A+ ++L ++++PV ++A +T A + +IA Sbjct: 117 RLSMDWRDARIRAVIALDLGPAAGFLPETLEQVKVPVLVLAAGVETPEIAAIKADSKYIA 176 Query: 199 QFLPRA-RV-RMIDGATHYTFLDTCVPAAMTI-------HATICKEAPGVDRDAVHA 246 +LP+A RV R I A+H++F+ C P I +C+ G DR A+HA Sbjct: 177 GYLPKATRVYREIPDASHFSFMQICKPNGEKIVEELSPGEGFVCRNGGGRDRAALHA 233 >all0969_2 [R] COG4188 Predicted dienelactone hydrolase Nos Nostoc sp. PCC 7120 Length = 372 Score = 92.8 bits (229), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 65/235 (27%), Positives = 109/235 (46%), Gaps = 32/235 (13%) Query: 1 VVFSHGTGGSADELDWLASAFAKHGYIVAGVNHPGNNALE-----------PLTREGFVL 49 VV SHG +LA A +G+ VA + HPG+NA P RE Sbjct: 72 VVISHGIASDRYSFIYLAEHLASYGFAVAVLEHPGSNAKRFEQYFAGLASPPEPRE---- 127 Query: 50 WWERATDLSEVLDAML----ADKAFGPHIDPRRVGAAGFSLGGYTVLELAGARTDLYAFR 105 + +R D+ +LD + +D ++ +++GA G S GGYTVL L GA+ + Sbjct: 128 FIDRPLDIKVLLDELQRLEKSDPRLQGKLNFQQIGAIGQSFGGYTVLSLGGAKINFNQL- 186 Query: 106 AYCHSPAADSNCHPPTNYASRKVAPMSASAIASMKRSGASYRDLRIKAVFAMAPAIGKAL 165 + +C+P + +S ++ + + +D RIKAV A+ P Sbjct: 187 --------NQDCNP--DNSSLNISLLLQCEANQLLPQDYQLQDSRIKAVIAINPISSSIF 236 Query: 166 DQQSLTRIEIPVRLIAGTADTTAPPTTNVLHIAQFLPR--ARVRMIDGATHYTFL 218 + +++I++PV ++AG+ D APP + +LP + +ID ATH+T + Sbjct: 237 GESGISKIKLPVMVVAGSQDIFAPPVPEQIRPFTWLPNPYKYLVLIDNATHFTLI 291