BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0197.p (438 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Z5523 [GEPR] COG0477 Permeases of the major facilitator superfam... 287 4e-77 ECs4893 [GEPR] COG0477 Permeases of the major facilitator superf... 287 4e-77 PA4343 [GEPR] COG0477 Permeases of the major facilitator superfa... 282 1e-75 PA0703 [GEPR] COG0477 Permeases of the major facilitator superfa... 278 1e-74 RSc3060 [GEPR] COG0477 Permeases of the major facilitator superf... 278 2e-74 >Z5523 [GEPR] COG0477 Permeases of the major facilitator superfamily EcZ Escherichia coli O157:H7 EDL933 Length = 438 Score = 287 bits (734), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 171/427 (40%), Positives = 249/427 (58%), Gaps = 12/427 (2%) Query: 19 VRKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFP-ATTPLASLLMTMATFGAG 77 V+K + RVI A GNALE FDF +++FF V I FFP + +LL+ ATFG Sbjct: 11 VKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVALLLAFATFGVS 70 Query: 78 FVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRL 137 F MRPLGG+++GA+ADRFGRKPAM+ +LM+ GT +IG+ P+Y++ G +V RL Sbjct: 71 FFMRPLGGIIVGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARL 130 Query: 138 LQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFL----- 192 +QG + GGEVGAS +LL E+A N RGF SW +A+QG A G + LS +L Sbjct: 131 IQGVAAGGEVGASMSLLVESAPANRRGFYSSWSLATQGLATTFGGVVALGLSAWLPFATG 190 Query: 193 SQQELDSWGWRLPFLFGLMLGPLGYAIR-SMLHETP-PIESRKGHAVRLVFSRFYH---P 247 S+ + WGWR+PF G++L P+G +R S+ ++ P P ++K A FS Sbjct: 191 SETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPEPAHNKKAAASESAFSLLMQHKAT 250 Query: 248 LITGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSD 307 + G+LL G T + +I +FY T+ L M + S LAG I VG+ G+L D Sbjct: 251 IANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCD 310 Query: 308 HLARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVE 367 + R+K ++ SR++ +P+FWLL + P L + + TLG LL+ E Sbjct: 311 SVGRKK-LILISRVMVLICSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISE 369 Query: 368 SFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIP 427 P+ +RA GF+LVYSIGV+IFGGFA + T I + +PA Y+ +++S++AL+ Sbjct: 370 LLPKRIRALGFALVYSIGVAIFGGFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLY 429 Query: 428 MKEHSTE 434 +KE + E Sbjct: 430 VKEPAKE 436 >ECs4893 [GEPR] COG0477 Permeases of the major facilitator superfamily Ecs Escherichia coli O157:H7 Length = 438 Score = 287 bits (734), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 171/427 (40%), Positives = 249/427 (58%), Gaps = 12/427 (2%) Query: 19 VRKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFP-ATTPLASLLMTMATFGAG 77 V+K + RVI A GNALE FDF +++FF V I FFP + +LL+ ATFG Sbjct: 11 VKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADKNVALLLAFATFGVS 70 Query: 78 FVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRL 137 F MRPLGG+++GA+ADRFGRKPAM+ +LM+ GT +IG+ P+Y++ G +V RL Sbjct: 71 FFMRPLGGIIVGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARL 130 Query: 138 LQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFL----- 192 +QG + GGEVGAS +LL E+A N RGF SW +A+QG A G + LS +L Sbjct: 131 IQGVAAGGEVGASMSLLVESAPANRRGFYSSWSLATQGLATTFGGVVALGLSAWLPFATG 190 Query: 193 SQQELDSWGWRLPFLFGLMLGPLGYAIR-SMLHETP-PIESRKGHAVRLVFSRFYH---P 247 S+ + WGWR+PF G++L P+G +R S+ ++ P P ++K A FS Sbjct: 191 SETVMAEWGWRVPFFIGVLLAPVGCWLRLSLENDVPEPAHNKKAAASESAFSLLMQHKAT 250 Query: 248 LITGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSD 307 + G+LL G T + +I +FY T+ L M + S LAG I VG+ G+L D Sbjct: 251 IANGILLAIGSTVATYISLFYYGTWAAKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCD 310 Query: 308 HLARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGSGASFLLLVE 367 + R+K ++ SR++ +P+FWLL + P L + + TLG LL+ E Sbjct: 311 SVGRKK-LILISRVMVLICSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISE 369 Query: 368 SFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIP 427 P+ +RA GF+LVYSIGV+IFGGFA + T I + +PA Y+ +++S++AL+ Sbjct: 370 LLPKRIRALGFALVYSIGVAIFGGFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLY 429 Query: 428 MKEHSTE 434 +KE + E Sbjct: 430 VKEPAKE 436 >PA4343 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 439 Score = 282 bits (721), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 165/418 (39%), Positives = 246/418 (58%), Gaps = 12/418 (2%) Query: 24 AKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVMRPL 83 ++ R +AA +GNALE +DF ++ F + +I + FFP+ SLLM +ATFG GF MRP+ Sbjct: 9 SRTRQVAAAVIGNALEWYDFIVYGFLSSLIARLFFPSGDEYTSLLMALATFGVGFFMRPV 68 Query: 84 GGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQGFSM 143 GG+++G YADR GRK AM L LM +I P+Y +IGV APLLIV R+LQGF+ Sbjct: 69 GGVLLGLYADRRGRKAAMQLIILLMTLSIAMIAFAPTYAAIGVGAPLLIVIARMLQGFAT 128 Query: 144 GGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSWGWR 203 GGE ++TA L E+A + RG SWQ+ Q A GA G L++ L + L+SWGWR Sbjct: 129 GGEYASATAFLVESAPPHRRGLYGSWQLFGQCLAVFAGAGMGALVTHCLDAEALESWGWR 188 Query: 204 LPFLFGLMLGPLGYAIRSMLHETPPI-----ESRKGHAVRLVFSRFYHPLITGVLLMTGG 258 +PFLFGL++GP+G IR + ET E + + V + ++ + L G Sbjct: 189 VPFLFGLLIGPVGLWIRRYMGETEAFLEAIREPGERQGLLGVLREYRRSVLVSMGLTVVG 248 Query: 259 TASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFVIF 318 T S ++V+ MPT+ L +P F+ A A++ + P AG LSD + RR P ++ Sbjct: 249 TVSFYVVLVNMPTFAHKQLGLPLDEVFMVQMAAVALMTLVIPLAGGLSDRVGRR-PVLLV 307 Query: 319 SRLLGASLIYPAF-WLLNHTAQPAFALVAIGTLAGCLTLGS--GASFLLLVESFPQAVRA 375 + L ++YP F W+ A P+ + + L C +G G + + E FP VR+ Sbjct: 308 ATLAFMLMVYPLFAWV---AAAPSLGRLLLMQLLLCTAIGGFFGPAPTAVAEQFPVRVRS 364 Query: 376 TGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKEHST 433 TG ++ Y++ V +FGGFAPF+VTWL EV G P++PA Y++ + + L+A + ++E +T Sbjct: 365 TGLAVAYNLAVMLFGGFAPFIVTWLTEVGGSPVAPAFYVLGAAFLGLLATLYLREGAT 422 >PA0703 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 438 Score = 278 bits (712), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 184/432 (42%), Positives = 265/432 (61%), Gaps = 16/432 (3%) Query: 2 KPSSAPAFSDADAADKVVRKSEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPAT 61 P+ PA AAD R+S + AV +GNA+E FDF +++ FAVMIG+ FFP+ Sbjct: 3 SPTRPPA-----AADPASRRS------VFAVVLGNAVEFFDFGVYATFAVMIGRTFFPSD 51 Query: 62 TPLASLLMTMATFGAGFVMRPLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSY 121 + SLL+++ FG GFV+RPLG +++GAYADR GRKPAM+L LMA GTG I V P Y Sbjct: 52 SAFVSLLLSVTAFGVGFVIRPLGAVLIGAYADRAGRKPAMLLTLFLMALGTGGIAVLPGY 111 Query: 122 DSIGVLAPLLIVTGRLLQGFSMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVG 181 DSIG APLL+V RLLQG + GGE G +T + EAA + RG WQ+ +QG AA+ Sbjct: 112 DSIGPAAPLLLVLTRLLQGLAWGGEAGPATTYILEAAPPHKRGTYACWQVVAQGIAAVAA 171 Query: 182 ALTGYLLSTFLSQQELDSWGWRLPFLFGLMLGPLGYAIRSMLHETPPIESRKGHAVRL-- 239 L GYLL+ +L +++L WGWR+PF GL++ P+G IR L ET R+ L Sbjct: 172 GLMGYLLTLWLDERQLQEWGWRIPFAAGLLVLPIGLYIRLNLAETFSGRGRQASTRNLLG 231 Query: 240 -VFSRFYHPLITGVLLMTGGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVG 298 +F L+ G+L+++G T + + + YM T+ + LH+P ++ L +AGA L + Sbjct: 232 ELFGNHRRALLLGLLILSGSTITQYFLN-YMTTFALTELHLPAGIAMLSTLVAGAALALS 290 Query: 299 SPFAGLLSDHLARRKPFVIFSRLLGASLIYPAFWLLNHTAQPAFALVAIGTLAGCLTLGS 358 + G+L D RR +I RL ++++PA + +PA L + L+ + Sbjct: 291 ALLGGVLCDRYGRRA-VLILPRLALLAVLFPALQAMTRHPEPAVFLAVLALLSALHGMSG 349 Query: 359 GASFLLLVESFPQAVRATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCS 418 A +LLVESFP+A+R+TGFSLVY+ GV+ FGG A VVTWLI V+G+P+SP Y+++ + Sbjct: 350 AALIVLLVESFPRALRSTGFSLVYATGVAAFGGTAQIVVTWLIGVTGNPLSPLGYLLLAN 409 Query: 419 IVSLIALIPMKE 430 +V L+ +E Sbjct: 410 LVCLVGAWLARE 421 >RSc3060 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 433 Score = 278 bits (711), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 172/422 (40%), Positives = 237/422 (56%), Gaps = 13/422 (3%) Query: 22 SEAKFRVIAAVTVGNALEMFDFTIFSFFAVMIGKQFFPATTPLASLLMTMATFGAGFVMR 81 +E+K R I V GN LEMFDF ++ ++A I FFPA SL++++ TFGAGF+MR Sbjct: 7 NESKARAIFRVVSGNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFGAGFLMR 66 Query: 82 PLGGLVMGAYADRFGRKPAMMLCTSLMAAGTGIIGVTPSYDSIGVLAPLLIVTGRLLQGF 141 PLG +V+GAY DR GR+ ++L LMA GT +I + P Y +IG+ APLL++ GRLLQGF Sbjct: 67 PLGAIVLGAYIDRHGRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIGRLLQGF 126 Query: 142 SMGGEVGASTALLAEAALRNSRGFMISWQMASQGAAALVGALTGYLLSTFLSQQELDSWG 201 S GGE G + L+E A +GF +SWQ SQ A + AL G LLS LS +E+ WG Sbjct: 127 SAGGEPGGVSVYLSEIATPGRKGFYVSWQSGSQQVAVMFAALLGVLLSFKLSPKEMGEWG 186 Query: 202 WRLPFLFGLMLGPLGYAIRSMLHETPPIESRK-----GHAVRLVFSRFYHPLITGVLLMT 256 WR+PFL G ++ P + IR L ET E RK G +R + G LL Sbjct: 187 WRVPFLIGCLIVPFLFFIRRSLRETEEFEQRKHRPTMGELLR-SMGENWRLAGAGTLLAV 245 Query: 257 GGTASMFIVVFYMPTYLVVGLHMPTSVSFLGACLAGAILLVGSPFAGLLSDHLARRKPFV 316 T S +++ Y PT+ LHM S + GA L+ P G LSD + RR P + Sbjct: 246 MTTVSFYLITAYTPTFGRNVLHMSDFDSLIVTLCVGASNLIWLPVMGSLSDRIGRRTPLI 305 Query: 317 IFSRLLGASLIYPAF-WLLNHTAQPAFA-LVAIGTLAGCLTLG-SGASFLLLVESFPQAV 373 +FS L + YPA WL+ A+P+F L+A+ L G +GA + L E P + Sbjct: 306 VFSALTLLT-AYPAMTWLI---AEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQL 361 Query: 374 RATGFSLVYSIGVSIFGGFAPFVVTWLIEVSGDPMSPAHYMIVCSIVSLIALIPMKEHST 433 R TGFSL YS+ +IFGGF P + T LI +G+ P ++ +I L+A + + + Sbjct: 362 RTTGFSLAYSLATAIFGGFTPAIATGLIHATGNKAMPGLWLSFAAICGLVATLTVVKPQP 421 Query: 434 EP 435 P Sbjct: 422 RP 423