BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0060.p (281 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO1566 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 438 e-123 BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 397 e-110 mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 386 e-107 AGc22 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene... 384 e-106 SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 382 e-106 >YPO1566 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Ype Yersinia pestis Length = 280 Score = 438 bits (1127), Expect = e-123, Method: Compositional matrix adjust. Identities = 203/281 (72%), Positives = 244/281 (86%), Gaps = 1/281 (0%) Query: 1 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60 MKLLRYGPVG+E+P +LD+ G+IRDLSAHI+D++G L A+L K+R+LD +LP+V G Sbjct: 1 MKLLRYGPVGEERPALLDELGEIRDLSAHIEDISGKFLLPATLYKLRQLDSASLPLVRGE 60 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120 R+GACVG++GKFIC+GLNYADHAAE+ IP EPV+FNKWTSA++GPND+++IPR S K Sbjct: 61 QRLGACVGHVGKFICVGLNYADHAAETGAEIPQEPVIFNKWTSAIIGPNDDIEIPRNSVK 120 Query: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELGV+IG GG YI E +AMS+VAGYCVVND+SERE+Q+E GGTWDKGKGCDTFGP Sbjct: 121 TDWEVELGVVIGTGGRYIGEANAMSYVAGYCVVNDISEREFQLEHGGTWDKGKGCDTFGP 180 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240 IGPWLVTRDE+ DP +LG+WL+VDG+RYQNG+T TMIF + I+SYLSRFMSLQ GDVIS Sbjct: 181 IGPWLVTRDEIPDPHQLGLWLDVDGRRYQNGSTRTMIFKIPQIISYLSRFMSLQSGDVIS 240 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQKTIDA 281 TGTPPGVG+G KP VYL AGQ +RLGI+GLGEQ QKTI A Sbjct: 241 TGTPPGVGLGQKP-PVYLHAGQIMRLGIEGLGEQCQKTIQA 280 >BMEI1708 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Bme Brucella melitensis Length = 301 Score = 397 bits (1021), Expect = e-110, Method: Compositional matrix adjust. Identities = 182/276 (65%), Positives = 226/276 (81%), Gaps = 1/276 (0%) Query: 1 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60 MK LRYG GQEKPG+LD G IRDLSAH+ D++GA LD +L K+ LD+N+LP VEGN Sbjct: 21 MKFLRYGAAGQEKPGLLDADGTIRDLSAHVSDLSGAALDPDALAKLGALDVNSLPKVEGN 80 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120 PRIG CV GKFICIGLNY+DHAAE+ +P+EP++F K TSA+VGPND++ IPRGS+K Sbjct: 81 PRIGPCVAGTGKFICIGLNYSDHAAETGAAVPSEPIIFMKATSAIVGPNDDLVIPRGSQK 140 Query: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELG+IIGK Y+ E+DA+ +VAGYC ++DVSER +QIER G W KGK CDTFGP Sbjct: 141 TDWEVELGIIIGKTAKYVSEEDALDYVAGYCTIHDVSERAFQIERQGQWTKGKSCDTFGP 200 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240 GPWLVT+DE+ADP+ L +WL+V+G+ QNG++ TMI+ V H+VSYLS+FMSLQPGD+IS Sbjct: 201 TGPWLVTKDEIADPENLAVWLKVNGETMQNGSSRTMIYGVRHLVSYLSQFMSLQPGDIIS 260 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQ 276 TGTPPGVGMG+KP YL+AG + LGI+GLG Q+Q Sbjct: 261 TGTPPGVGMGMKPPR-YLKAGDVVELGIEGLGSQKQ 295 >mll4780 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mlo Mesorhizobium loti Length = 281 Score = 386 bits (992), Expect = e-107, Method: Compositional matrix adjust. Identities = 179/276 (64%), Positives = 218/276 (78%), Gaps = 1/276 (0%) Query: 1 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60 MKLLRYG VG E+PG+LD G IRDLSAH+ D+ G L ASLE + KLD +LP V G Sbjct: 1 MKLLRYGEVGSERPGLLDADGTIRDLSAHVADIAGTTLHPASLEMLGKLDPKSLPAVSGK 60 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120 PR+GACV GKFICIGLNY+DHAAE+ +P EP++F K +SA+VGP+D+V IPRGS+K Sbjct: 61 PRLGACVAGTGKFICIGLNYSDHAAETGATVPPEPIIFMKASSAIVGPDDDVLIPRGSEK 120 Query: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVEL V+IGK Y+ E DA+ +VAGY V +DVSER +Q ER G W KGK CDTFGP Sbjct: 121 TDWEVELAVVIGKTAKYVSEADALDYVAGYAVAHDVSERAFQAERQGQWTKGKSCDTFGP 180 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240 GPWLVT+DEVADPQ L MWL V+GK QNG+T TM++ V ++VSYLS+FMSL PGD+IS Sbjct: 181 TGPWLVTKDEVADPQNLKMWLTVNGKTMQNGSTKTMVYGVKYLVSYLSQFMSLHPGDIIS 240 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQ 276 TGTPPGVG+G+KP V+LRAG + LGI+GLG+Q+Q Sbjct: 241 TGTPPGVGLGMKP-PVFLRAGDVVELGIEGLGQQKQ 275 >AGc22 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 280 Score = 384 bits (987), Expect = e-106, Method: Compositional matrix adjust. Identities = 180/279 (64%), Positives = 218/279 (78%), Gaps = 2/279 (0%) Query: 1 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60 MKL+R G GQEKP ILD GK+RDLSAH+KD+ G + L+KI +DL TLPV+ Sbjct: 1 MKLMRVGQPGQEKPAILDAEGKVRDLSAHVKDIGGDAISPEGLKKIAAIDLATLPVLS-E 59 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120 RIGACV GKFICIGLN++DHAAE+ +P EPV+F K TSA+VGPNDNV IPRGS+K Sbjct: 60 ERIGACVAGTGKFICIGLNFSDHAAETGATVPPEPVIFMKATSAIVGPNDNVTIPRGSEK 119 Query: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELGV+IGK Y+ E DA+ +VAGYCV +DVSER +Q ER G W KGK CDTFGP Sbjct: 120 TDWEVELGVVIGKTAKYVSEADALDYVAGYCVSHDVSERAFQTERAGQWTKGKSCDTFGP 179 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240 IGPWLVT+DE+ DPQ LGMWL+V+G+ Q+G++ TM++ VAH+VSYLS+FMSL PGDVIS Sbjct: 180 IGPWLVTKDEITDPQNLGMWLKVNGQTMQDGSSKTMVYGVAHVVSYLSQFMSLHPGDVIS 239 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQKTI 279 TGTPPGVGMG KP YL+ G + LGI+GLG Q+Q + Sbjct: 240 TGTPPGVGMGQKPPR-YLKTGDVVELGIEGLGSQKQTFV 277 >SMb21112 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Sme Sinorhizobium meliloti Length = 281 Score = 382 bits (982), Expect = e-106, Method: Compositional matrix adjust. Identities = 174/277 (62%), Positives = 219/277 (79%), Gaps = 1/277 (0%) Query: 1 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60 MKLLRYG GQEKPG+L G IRDLS H+ D+ LD + L+++ LD+ TLP V GN Sbjct: 1 MKLLRYGEPGQEKPGLLGSDGIIRDLSGHVSDLAAGALDPSKLDELANLDVETLPAVSGN 60 Query: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120 PR+G CV GKFICIGLNY+DHAAE+ +P EP++F K TSA+VGPND++ +PRGS+K Sbjct: 61 PRLGPCVAGTGKFICIGLNYSDHAAETGATVPPEPIIFMKATSAIVGPNDDLVLPRGSEK 120 Query: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 TDWEVELG++IGK Y+ E +A+ +VAGYC V+DVSER +Q ER G W KGK CDTFGP Sbjct: 121 TDWEVELGIVIGKTAKYVSEAEALDYVAGYCTVHDVSERAFQTERHGQWTKGKSCDTFGP 180 Query: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240 GPWLVT+DEVADPQ L MWL+V+G+ Q+G+T TM++ AH+VSYLS+FMSL+PGD+IS Sbjct: 181 TGPWLVTKDEVADPQDLAMWLKVNGETMQDGSTKTMVYGAAHLVSYLSQFMSLRPGDIIS 240 Query: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQK 277 TGTPPGVGMG+KP YL+AG + LGI+GLG Q+Q+ Sbjct: 241 TGTPPGVGMGMKPPR-YLKAGDVVELGIEGLGSQKQR 276