BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0021.p (443 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1887_1 [R] COG1073 Hydrolases of the alpha/beta superfamily R... 558 e-159 CAC3407 [R] COG1073 Hydrolases of the alpha/beta superfamily Cac... 167 5e-41 CC0882 [R] COG1073 Hydrolases of the alpha/beta superfamily Ccr ... 118 3e-26 AGl2362 [R] COG1073 Hydrolases of the alpha/beta superfamily Atu... 86 2e-16 mlr4383 [R] COG1073 Hydrolases of the alpha/beta superfamily Mlo... 82 2e-15 >RSc1887_1 [R] COG1073 Hydrolases of the alpha/beta superfamily Rso Ralstonia solanacearum Length = 447 Score = 558 bits (1439), Expect = e-159, Method: Compositional matrix adjust. Identities = 294/446 (65%), Positives = 339/446 (76%), Gaps = 11/446 (2%) Query: 2 PNFSIEVSPADDLIDVPRRIIVRGAHPGEPVRISSATLR-NGTQWRSTACYIADAHGNVD 60 P F++ V+PADDLIDVPRRI+V G PG V +++ TLR + W S A +IADA G VD Sbjct: 5 PAFALSVAPADDLIDVPRRIVVTGLVPGAQVDLAARTLRGHAVPWCSRAAFIADADGTVD 64 Query: 61 VSMNPAIDGDYYGIDPMGLLWSQKPEQAGRRDQFHADVMAPLETTLTVSALEQPGLPPCT 120 +S + + GDY G+DPMGL+WSQ+PE R+ F + PL TTLT +A T Sbjct: 65 LSRDAPVCGDYTGVDPMGLVWSQRPEGGTSREVFASAATEPLTTTLTATAH-------GT 117 Query: 121 PASTRLVQRLAGAGVTRREVRELGLVGTLFLP---ATPGPHPAVMILNGSGGGINEPRAA 177 PA R VQRLA G+TR +VR+ GLVGTL+LP A PGP PAVM+LNGSGGGINEPRAA Sbjct: 118 PACARFVQRLAAPGLTRHDVRDDGLVGTLYLPDPHAHPGPRPAVMVLNGSGGGINEPRAA 177 Query: 178 LYASRGYAAFALAYFKAPGLSDYISNTPLEYFERGLDWLRAEVRPLHDFVAISGQSRGGE 237 LYAS GYAAFALAYFKAPGLSDYISNTPLEYFERGL WLR VRPLHDFVA+SGQSRGGE Sbjct: 178 LYASHGYAAFALAYFKAPGLSDYISNTPLEYFERGLAWLRRRVRPLHDFVAVSGQSRGGE 237 Query: 238 LVLLLGATFPQKVSAVLAYVPSALVHSGQNAADPAIGREGPTWLLRGQPLPHQWQDNRTA 297 L LLLGATFP VSAV+ YVP A+VHS QNAADPAIGREGPTWL RG+PLPH W+ NRTA Sbjct: 238 LALLLGATFPAAVSAVIGYVPGAVVHSAQNAADPAIGREGPTWLYRGRPLPHLWEGNRTA 297 Query: 298 SWAPFDEGPAPHRHELAMLTALQDEEAVARARIPVEKIAAPVLLLSATDDGSWPSSLYCG 357 +WAPFDEG PHRHE A+ TAL+D +AV RARI VE+ PVLLLSATDDGSWPSS Y Sbjct: 298 TWAPFDEGEPPHRHERAIRTALRDAQAVERARIRVEQTRGPVLLLSATDDGSWPSSDYAR 357 Query: 358 MVRDKLAEVAHPWPVLWRNYANAGHSILFPFVPTTQTTYAHPVSGRISTGGGEAAANARA 417 M +LAE HP+PV+ ++A AGH+I+FP+VPTTQ +AHPVSGRISTGGGE ANARA Sbjct: 358 MAAARLAEARHPYPVVHHDFAGAGHAIVFPYVPTTQLVHAHPVSGRISTGGGEPRANARA 417 Query: 418 DLDSWQQAQHFLRDALTRHSSPKETS 443 DL SW + FL +A+ S P S Sbjct: 418 DLQSWAAVRRFLAEAVAARSRPVSAS 443 >CAC3407 [R] COG1073 Hydrolases of the alpha/beta superfamily Cac Clostridium acetobutylicum Length = 431 Score = 167 bits (422), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 126/459 (27%), Positives = 206/459 (44%), Gaps = 59/459 (12%) Query: 1 MPNFSIEVSPADDLIDVPRRIIVRGAHPGEPVRISSATL-------RNG----------T 43 M N I + P +ID I+V+G P E + I + +L +N T Sbjct: 1 MKNVKIIIEPQCSMIDEKTNILVQGLVPYEIITIKALSLDYYRIGAKNNNPDNWKWVKKT 60 Query: 44 QWRSTACYIADAHGNVDVSMNPAIDGDYYGIDPMGLLWS----QKPEQAGRRDQFHADVM 99 + S A + AD G VD+S + I G Y +D MGL WS Q+ + ++ + Sbjct: 61 NFESFAVFKADDKGTVDLSKDAPIKGSYTRVDSMGLFWSMVSTQQQDYTLKKSLSSCPFL 120 Query: 100 APLETTLTVSALEQPGLPPCTPASTRLVQRLAGAGVTRREVRELGLVGTLFLPATPGPHP 159 +E L V + S++ + + V + V E LVG F Sbjct: 121 TSMEVILKVEVCGKE------LVSSKCKKLFVSSDVKCQYVTENNLVGKYFTNKNSKIKK 174 Query: 160 AVMILNGSGGGINEPR--AALYASRGYAAFALAYFKAPGLSDYISNTPLEYFERGLDWL- 216 +++L+GS GGI+ AAL AS GY + ALAYF L ++ PLEYFE+ +DW+ Sbjct: 175 GIIVLSGSEGGIHNASQIAALLASHGYPSLALAYFGMDTLPATLNEIPLEYFEKAIDWII 234 Query: 217 -RAEVRPLHDFVAISGQSRGGELVLLLGATFPQKVSAVLAYVPSALVHSGQNAADPAIGR 275 + ++ P + + + G+SRGGEL LLLG+TF +K+++V+A S ++ G N + Sbjct: 235 TKGDISP--NNLTVFGRSRGGELALLLGSTF-KKITSVIAVAASPILF-GTNTTKLS--- 287 Query: 276 EGPTWLLRGQPLPHQWQDNRTASWAPFDEGPAPHRHELAM----LTALQDEEAVARARIP 331 +W +G+ +P+ S+ H + A+++ +A+ IP Sbjct: 288 ---SWKYKGEDIPYLNLKINIISFLKNALTDKLHGRTINFSKLYTNAIKNPDAIETYMIP 344 Query: 332 VEKIAAPVLLLSATDDGSWPSSLYCGMVRDKLAEVAHPWPVLWRNYANAGHSILFPFVPT 391 +E +L+LS +D WPSS C + ++L + + + Y AGH I FP+ P Sbjct: 345 IENTNGSILMLSGDNDHIWPSSKLCKLGIERLKQHNFSFYYDFLEYK-AGHHIYFPYQP- 402 Query: 392 TQTTYAHPVSGRISTGGGEAAANARADLDSWQQAQHFLR 430 I G A ++ +SW + FL+ Sbjct: 403 ------------IYKYNGSNENMAISNRESWNKILEFLQ 429 >CC0882 [R] COG1073 Hydrolases of the alpha/beta superfamily Ccr Caulobacter vibrioides Length = 323 Score = 118 bits (296), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 101/309 (32%), Positives = 137/309 (44%), Gaps = 28/309 (9%) Query: 135 VTRREVRELGLVGTLFLPATPGPHPAVMILNGSGGGINEPR--AALYASRGYAAFALAYF 192 T +EV E GLV P TPG HPA++IL GS GG R A +A +GYA AL+YF Sbjct: 35 TTAKEVGEQGLVAQFLQPTTPGKHPALLILGGSEGGSAGVRQLAKPFAEQGYAVLALSYF 94 Query: 193 KAPGLSDYISNTPLEYFERGLDWLRAEVRPLHDFVAISGQSRGGELVLLLGATFPQKVSA 252 APGL PLEYF+R + WL A+ + + G S+G E LL+ + P + A Sbjct: 95 AAPGLPATAEELPLEYFDRAVAWLAAQPSVDPKAIGVYGVSKGAEASLLVASRNPA-LKA 153 Query: 253 VLAYVPSALVHSGQNAADPAIGREGPTWLLRGQPLPHQWQDNRTASWAPFDEG----PAP 308 A V ++ V G + G +W L G+P+ + P+ G P+ Sbjct: 154 AGAGVGTSFVWQGISTTG---GAAKSSWTLAGKPV----------DYLPYGPGVRFDPSN 200 Query: 309 HRHELAML--TALQDEEAVARARIPVEKIAAPVLLLSATDDGSWPSSLYCGMVRDKLAEV 366 + + L T L+ +A +A IPVEKI PV+L+S D W SS V +L Sbjct: 201 YMAFIYGLYDTGLKAADAHPQAAIPVEKIHGPVMLISGRADAMWSSSAMSDAVVARLKAK 260 Query: 367 AHPWPVLWRNYANAGHSILFPFVPTTQTTYAHPVSGRISTGGGEAAANARADLDSWQQAQ 426 V Y +AGH+ P + G GG N A D W + Sbjct: 261 GFAHKVSHLAYPDAGHTAGMPALMGGSD------KGADEAVGGTVEGNRFARTDMWPKLV 314 Query: 427 HFLRDALTR 435 F AL R Sbjct: 315 CFFDGALKR 323 >AGl2362 [R] COG1073 Hydrolases of the alpha/beta superfamily Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 296 Score = 85.5 bits (210), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 83/298 (27%), Positives = 124/298 (41%), Gaps = 29/298 (9%) Query: 139 EVREL-GLVGTLFLPATPGPHPAVMILNGSGGGINEPRAALYASRGYAAFALAYFKAPGL 197 +V +L G V L T V++L GS G ++ RA L+A+ G A AL +F Sbjct: 21 DVEQLEGKVRGALLQGTNSGKTGVIVLGGSSGRVDTTRAGLFANTGARALALQWFGGANQ 80 Query: 198 SDYISNTPLEYFERGLDWLRAEVRPLHDFVAISGQSRGGELVLLLGATFPQKVSAVLAYV 257 I PLE F D L + D G S+G E LL +T +++ V+A Sbjct: 81 PPGICEIPLETFVDATDLL---ISRGCDRTVFVGTSKGAE-AALLASTIDHRINVVIAIS 136 Query: 258 PSALVHSGQNAADPAIGR-EGPTWLLRGQPLPH-----QWQDNRTASWAPFDEGPAPHRH 311 PS++V A I E +W + PL WQ + + +G +R Sbjct: 137 PSSVVLGNIGAGVDGISWPERSSWSFKRSPLDFVPADINWQKD-------YRDGLVSYRS 189 Query: 312 ELAMLTALQDEEAVARARIPVEKIAAPVLLLSATDDGSWPSSLYCGMVRDKLAEVAHPWP 371 + D + RARIPVE A ++L++ DD WPS + + + AH Sbjct: 190 FFEKCLS-NDVQLNERARIPVESSPADLILVAGGDDALWPSGYFARQILQ--SRQAHGRQ 246 Query: 372 VLWRNYANAGHSILFPFVPTTQTTYAHPVSGRISTGGGEAAANARADLDSWQQAQHFL 429 +AGH IL P TTQ + H GG A+A+ ++W+ + L Sbjct: 247 ATLIFDKDAGHRILLPG-ETTQRSKLH-------AHGGSDEADAKLGQNAWRAIRELL 296 >mlr4383 [R] COG1073 Hydrolases of the alpha/beta superfamily Mlo Mesorhizobium loti Length = 298 Score = 82.4 bits (202), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 88/293 (30%), Positives = 132/293 (45%), Gaps = 22/293 (7%) Query: 139 EVRELGLVGTLFLPATPGPHPAVMILNGSGGGINEPRAALYASRGYAAFALAYFKAPGLS 198 E+ G+ GTL A V++L+GS G ++ RA L+AS G +A AL +F G Sbjct: 26 EILSGGVAGTLLQCADA--MTGVVVLSGSSGRVDAARAKLFASEGVSALALQWFGGEGQV 83 Query: 199 DYISNTPLEYFERGLDWLRAEVRPLHDFVAISGQSRGGELVLLLGATFPQKVSAVLAYVP 258 I PLE F D L V + G S+ G LL A Q++ AV+A P Sbjct: 84 PGICEIPLEIFFAATDHL---VGLGCRRIVYVGTSK-GAEAALLAAAHDQRIDAVIAISP 139 Query: 259 SALVHSGQNAADPAIGR-EGPTWLLRGQPLPHQWQDNRTASWAPFDEGPAPHRHELAMLT 317 +++V I E +W L+G PLP + R A +G +R Sbjct: 140 TSVVWGNIGPGRDGISWPERSSWTLKGVPLP--FVPTRPNWRASHVDGLVSYRGLFQHCL 197 Query: 318 ALQDEEAVARARIPVEKIAAPVLLLSATDDGSWPSSLYCGMVRDKLAEVAHPWPVLWRNY 377 A +EA RA IPVE I+A ++L++ DD WPS + + + + ++ + Sbjct: 198 ASFGQEA-DRAAIPVEAISAKLVLVAGGDDALWPSLDFAQSIVRRREDAGKATRLV--SS 254 Query: 378 ANAGHSILFPFVPTTQTT-YAHPVSGRISTGGGEAAANARADLDSWQQAQHFL 429 +AGH IL P T ++ +AH GG A+AR D+W+ + L Sbjct: 255 PDAGHRILLPGETTPRSALHAH---------GGNDHADARLGRDAWELIRDLL 298