BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0162.p (398 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2450 [I] COG1398 Fatty-acid desaturase Rso Ralstonia solanace... 556 e-158 PA0286 [I] COG1398 Fatty-acid desaturase Pae Pseudomonas aeruginosa 417 e-116 all1599 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 89 1e-17 sll0541 [I] COG1398 Fatty-acid desaturase Syn Synechocystis 89 1e-17 all4991 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 89 2e-17 >RSc2450 [I] COG1398 Fatty-acid desaturase Rso Ralstonia solanacearum Length = 398 Score = 556 bits (1434), Expect = e-158, Method: Compositional matrix adjust. Identities = 257/396 (64%), Positives = 325/396 (82%), Gaps = 1/396 (0%) Query: 3 LDAILDFVANGLLHASGWQIVIYTLVMTHITIVGVTLYLHRCQAHRALELHAIPSHFFRL 62 +D+ L F+ANG L+ + WQI+I+TLV+TH+TI VT++LHRCQAHRAL+LH +H FR Sbjct: 4 IDSFLSFLANGTLNWAWWQILIFTLVVTHMTIASVTIFLHRCQAHRALDLHPAAAHVFRF 63 Query: 63 WLWMTTGMVTKQWAAIHRKHHAKCETEEDPHSPQTRGIKKVMWEGAELYRAESKNDETME 122 WLW+TTGMVTK+WAAIHRKHHAKCETEEDPHSPQTRGI+KV+ EGAELYRAE+KN ET++ Sbjct: 64 WLWLTTGMVTKEWAAIHRKHHAKCETEEDPHSPQTRGIRKVLLEGAELYRAEAKNRETIQ 123 Query: 123 KFGHGTPDDWLEHNVYSKYSWQGVGLMLIIDVLLFGAIGLTIWAVQMAWIPFWAAGVVNG 182 KFGHGTP+DW+E N+YSKY+WQGVGLMLI+DVL+FG +GLT+WAVQM WIP AAG++NG Sbjct: 124 KFGHGTPNDWIEQNLYSKYTWQGVGLMLILDVLMFGTLGLTVWAVQMLWIPIHAAGIING 183 Query: 183 LGHFWGYRNYDCVDASTNLFPIGILIGGEEMHNNHHTFGTSAKFSAKWYEFDVGWMYIRI 242 LGH+WGYRN+DC DASTN+FP G++IGGEE+HNNHHT+ TSAKFS KWYEFDVGW YIR Sbjct: 184 LGHYWGYRNFDCEDASTNVFPWGLIIGGEELHNNHHTYATSAKFSIKWYEFDVGWGYIRA 243 Query: 243 LETFGLAKVKKVLPVPKFDRQKAVIDFDTLQSVIANRYDVTAKYARSLKSAWKDELDHLI 302 LE GLAKVKKV P +F K V D +TL+++IANRYDV A+YA+++K A++ EL L Sbjct: 244 LEMVGLAKVKKVAPKARFGDVKPV-DQNTLEAIIANRYDVMARYAKTVKVAYRQELAKLK 302 Query: 303 EKAKLESRFLKSSKKLLQREPGRLEDGQKQQLSELFAHSKALQTMHEMRIELSAIWERSH 362 + +E R LK ++K R+ +L Q++QLS++ + AL T EMR +L A+W RS+ Sbjct: 303 DARDVEYRALKPARKWFHRDEAKLGAPQREQLSQIVSDHSALHTFVEMRRDLGALWGRSN 362 Query: 363 STREQLLQQLQDWCARAEASGVKALRDFALRLRSYA 398 +TREQLL QLQ+WC RAE SG+ AL++FA+RLR YA Sbjct: 363 ATREQLLHQLQEWCKRAEESGIHALQEFAVRLRRYA 398 >PA0286 [I] COG1398 Fatty-acid desaturase Pae Pseudomonas aeruginosa Length = 392 Score = 417 bits (1071), Expect = e-116, Method: Compositional matrix adjust. Identities = 200/388 (51%), Positives = 272/388 (70%), Gaps = 4/388 (1%) Query: 12 NGLLHASGWQIVIYTLVMTHITIVGVTLYLHRCQAHRALELHAIPSHFFRLWLWMTTGMV 71 NG L S WQ+V TLV+THITIV VT+YLHR AHRAL+LH HFFR WLW+TTGM Sbjct: 4 NGFLDLSPWQLVAVTLVLTHITIVSVTVYLHRYSAHRALDLHPALQHFFRFWLWLTTGMN 63 Query: 72 TKQWAAIHRKHHAKCETEEDPHSPQTRGIKKVMWEGAELYRAESKNDETMEKFGHGTPDD 131 T++W AIHRKHHAKCET EDPHSP +G+ V+ GAELY+AE+KN +T+ +G PDD Sbjct: 64 TREWTAIHRKHHAKCETVEDPHSPVHKGLFTVLRAGAELYKAEAKNQDTLRIYGKNCPDD 123 Query: 132 WLEHNVYSKYSWQGVGLMLIIDVLLFGAIGLTIWAVQMAWIPFWAAGVVNGLGHFWGYRN 191 W+E N+YS++ GV LM +ID+ LFGA+GLT+WAVQM WIPFWAAGVVNGLGH GYRN Sbjct: 124 WIERNLYSRFPIGGVVLMALIDLALFGALGLTVWAVQMVWIPFWAAGVVNGLGHAVGYRN 183 Query: 192 YDCVDASTNLFPIGILIGGEEMHNNHHTFGTSAKFSAKWYEFDVGWMYIRILETFGLAKV 251 ++C DA+TNL P GILIGGEE+HNNHHT+ SAK S K +EFD+GW +I++ LA+V Sbjct: 184 FECRDAATNLVPWGILIGGEELHNNHHTYPNSAKLSVKKWEFDLGWAWIKLFSFLRLARV 243 Query: 252 KKVLPVPKFDRQKAVIDFDTLQSVIANRYDVTAKYARSLKSAWKDELDHLIEKAKLESRF 311 +V P+ K +D DT +++ NR+ + A+Y R L A + + KA R Sbjct: 244 ARVAPIAHRVEGKHSLDMDTAMAILNNRFQIMAQY-RKLVIA--PLVKQEVAKADESVRH 300 Query: 312 L-KSSKKLLQREPGRLEDGQKQQLSELFAHSKALQTMHEMRIELSAIWERSHSTREQLLQ 370 L + +K+LL RE L+D ++ + AHS+AL+ ++E R+ L IW ++ + +L Sbjct: 301 LFRRAKRLLSRETSLLQDRHHVRIDSMLAHSQALKVIYEKRLALQQIWAKTSANGHDMLA 360 Query: 371 QLQDWCARAEASGVKALRDFALRLRSYA 398 ++DW AEASG+++L++FA +L++Y+ Sbjct: 361 AIKDWVHEAEASGIQSLKEFAAQLKTYS 388 >all1599 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 Length = 272 Score = 89.4 bits (220), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 71/263 (26%), Positives = 118/263 (44%), Gaps = 26/263 (9%) Query: 1 MTLDAILDFVANGLLHASGWQIVIYTLVMTHITI-VGVTLYLHRCQAHRALELHAIPSHF 59 + + A+ F+ + W V L++ IT +G+TL HR HR+ + +F Sbjct: 22 LHIGALFAFIPSNF----SWAAVGVALLLYWITGGLGITLGFHRLVTHRSFQTPKWLEYF 77 Query: 60 FRLWLWMTTGMVTKQWAAIHRKHHAKCETEEDPHSPQTRGIKKVMWE--GAELYRAESKN 117 L + +W HR HH +T+ DPH K W G +Y + S Sbjct: 78 LVLCGTLACQGGPIEWVGTHRIHHLHSDTDPDPHDSN----KGFWWSHIGWLIYHSPSHA 133 Query: 118 DETMEKFGHGTPDDWLEHNVYSKYSWQGVGLMLII------DVLLFGAIGLTIWAVQMAW 171 D + +F +D + + + + + L L++ +++G +W W Sbjct: 134 D--VPRFTKDIAEDPVYQFLQKYFIFIQIALGLLLLYLGGWSFVVWGVFFRIVWVYHCTW 191 Query: 172 IPFWAAGVVNGLGHFWGYRNYDCVDASTNLFPIGILIGGEEMHNNHHTFGTSAKFSAKWY 231 + VN H +GYR YD D STN + + +L+ GE HNNHH F SA+ +W+ Sbjct: 192 L-------VNSATHKFGYRTYDAGDRSTNCWWVAVLVFGEGWHNNHHAFQYSARHGLEWW 244 Query: 232 EFDVGWMYIRILETFGLAKVKKV 254 E D+ WM +++L+ GLA K+ Sbjct: 245 EVDLTWMTVQLLQILGLATNVKL 267 >sll0541 [I] COG1398 Fatty-acid desaturase Syn Synechocystis Length = 318 Score = 89.4 bits (220), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 66/226 (29%), Positives = 103/226 (45%), Gaps = 13/226 (5%) Query: 35 VGVTLYLHRCQAHRALELHAIPSHFFRLWLWMTTGMVTKQWAAIHRKHHAKCETEEDPHS 94 +G+TL HRC +HR+ + + F + + +W +HR HH +T DPH Sbjct: 94 IGITLGFHRCISHRSFNVPKWLEYIFVICGTLACQGGVFEWVGLHRMHHKFSDTTPDPHD 153 Query: 95 PQTRGIKKVMWE--GAELYRAESKNDETMEKFGHGTPDDWLEHNVYSKYSWQGVGLMLII 152 K W G ++ +K D + ++ DD + V L LI+ Sbjct: 154 SN----KGFWWSHIGWMMFEIPAKAD--IPRYTKDIQDDKFYQFCQNNLILIQVALGLIL 207 Query: 153 DVLLFGAIGLTIWA--VQMAWIPFWAAGVVNGLGHFWGYRNYDCVDASTNLFPIGILIGG 210 L G IW V++ ++ F VN H +GY +++ D S N + + +L G Sbjct: 208 FAL--GGWPFVIWGIFVRLVFV-FHFTWFVNSATHKFGYVSHESNDYSRNCWWVALLTFG 264 Query: 211 EEMHNNHHTFGTSAKFSAKWYEFDVGWMYIRILETFGLAKVKKVLP 256 E HNNHH + SA+ +W+E D+ WM I+ L GLAK K+ P Sbjct: 265 EGWHNNHHAYQYSARHGLQWWEVDLTWMTIKFLSLLGLAKDIKLPP 310 >all4991 [I] COG1398 Fatty-acid desaturase Nos Nostoc sp. PCC 7120 Length = 285 Score = 89.0 bits (219), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 79/240 (32%), Positives = 118/240 (49%), Gaps = 38/240 (15%) Query: 35 VGVTLYLHRCQAHRALEL-----HAIPSHFFRLWLWMTTGMVTKQ-----WAAIHRKHHA 84 +G+ L HR +H++ ++ +AI T G + Q W HR+HHA Sbjct: 58 IGICLGYHRLLSHKSFQVPKWLEYAI----------ATIGALAMQGGPIFWIGGHRQHHA 107 Query: 85 KCE-TEEDPHSPQTRGI--KKVMWEGAELY-RAESKNDETMEKFGHGTPDDWLEHNVYSK 140 E DP+S Q RG ++W LY R+E + E +K+ PD L + + Sbjct: 108 HTEDVYLDPYSSQ-RGFWWSHMLWI---LYPRSEFFDYEIYQKYA---PD--LARQPFYR 158 Query: 141 YSWQGVGLMLIIDVLLFGAIG---LTIWAVQMAWIPFW-AAGVVNGLGHFWGYRNYDCVD 196 + + L+ I L+ AIG I+ V + + W + VN H WGYR ++ D Sbjct: 159 WLDRYFLLLQIPFGLMLYAIGGWSFVIYGVVLRAVLLWHSTWFVNSATHMWGYRTFNADD 218 Query: 197 ASTNLFPIGILIGGEEMHNNHHTFGTSAKFSAKWYEFDVGWMYIRILETFGLAKVKKVLP 256 + NL+ + I+ GE HNNHHT+ AK +W+E DV W I++L+T GLAK K VLP Sbjct: 219 NARNLWWVSIVTYGEGWHNNHHTYPNVAKAGFQWWEVDVTWWSIKLLQTLGLAK-KVVLP 277