BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0114.p (403 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 376 e-104 SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carbox... 359 6e-99 AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 348 9e-96 AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 348 1e-95 AGl2386 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 344 2e-94 >RSc2871 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Rso Ralstonia solanacearum Length = 396 Score = 376 bits (965), Expect = e-104, Method: Compositional matrix adjust. Identities = 186/384 (48%), Positives = 242/384 (63%), Gaps = 8/384 (2%) Query: 15 RAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAGADPQRG 74 +A LRRD+HAHPEL FEE RT+D+V +L G V RGLG TG+V + + QR Sbjct: 11 QAEIQALRRDIHAHPELCFEEQRTSDLVAAKLAEWGIEVHRGLGKTGLVGVIRNGEGQR- 69 Query: 75 IVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLPGSVHFV 134 I LRAD+DALP+ EAN F H S G MHACGHDGHT MLLGAA L + G+VH + Sbjct: 70 IGLRADMDALPLAEANQFTHRSRHEGKMHACGHDGHTAMLLGAAHYLARHRNFSGTVHLI 129 Query: 135 FQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRSGPIMAAGSRLKITI 194 FQP EEGG GAR+MI DGLF++FP +AVFGMHNWPG+P G FG R GP+MA+ + +I I Sbjct: 130 FQPAEEGGGGAREMIRDGLFDRFPCDAVFGMHNWPGVPVGAFGTRVGPLMASSNEFRIVI 189 Query: 195 TGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDR 254 GKGAHAA PH G DP+ + MV Q + R+K P+D AV+S+ FHAGD N+IP+ Sbjct: 190 KGKGAHAALPHNGNDPVFVGAQMVSALQGVITRNKRPIDTAVLSITQFHAGDASNIIPNE 249 Query: 255 AELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVDFFQYYPATINTPAETDFCER 314 A + GT+RT S+ + ++ + + A+A AY ++ F + YP T+NT ET F Sbjct: 250 AWIGGTVRTFSTAVLDLIERRMEEVAKAIAAAYDCSIDFTFHRNYPPTVNTERETLFAAE 309 Query: 315 VIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHS-------LHHP 367 V+R+ G D + I M +EDF FML E+PG + IGN D LH+P Sbjct: 310 VMRELVGPDHVDANIDPTMGAEDFSFMLIEKPGCFAFIGNGDGDHREQGHGLGPCMLHNP 369 Query: 368 HYDFNDDIIEAGVRYWVALAQGYF 391 YDFND+++ G YWV L + + Sbjct: 370 SYDFNDELLPLGATYWVRLVEKFL 393 >SMc02256 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Sme Sinorhizobium meliloti Length = 389 Score = 359 bits (922), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 177/371 (47%), Positives = 235/371 (63%), Gaps = 1/371 (0%) Query: 21 LRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAGADPQRGIVLRAD 80 +RRDLHAHPEL EE RT+ + R L+ LGY V G+ TGVV +L R I +RAD Sbjct: 17 IRRDLHAHPELGLEETRTSAFIARHLEELGYEVATGIAKTGVVGTLRNGTGSRSIGIRAD 76 Query: 81 LDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLPGSVHFVFQPGEE 140 +DALPI E A+AS G+MHACGHDGHT MLLGAAR L + G++H +FQP EE Sbjct: 77 IDALPIQEETGVAYASTKPGLMHACGHDGHTAMLLGAARALAERRNFDGTIHLIFQPAEE 136 Query: 141 GGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRSGPIMAAGSRLKITITGKGAH 200 GA+ M+D+GLF++FP +AVF +HN P LP GQF LR GPIMAA +IT+ G+G H Sbjct: 137 NAGGAKIMVDEGLFDRFPCDAVFALHNEPNLPFGQFALREGPIMAAVDEARITVHGRGGH 196 Query: 201 AAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRAELRGT 260 A+P DPI S+V+ QTI AR+ P+DP+V++V FHAG N+IP+RAE+ Sbjct: 197 GAEPQATADPIVCGASIVMALQTIVARNIHPMDPSVVTVGAFHAGSASNIIPERAEIVVG 256 Query: 261 IRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVDFFQYYPATINTPAETDFCERVIRQTF 320 IR+ ++ +L+ IR + A A ++G + VD+ + Y ATIN AETDF + Sbjct: 257 IRSFDPAVRDELERRIRMIAEAQASSFGMRATVDYERSYDATINHKAETDFLREAAIRFA 316 Query: 321 GDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHSLHHPHYDFNDDIIEAGV 380 G D++ M SEDF +ML+ERPG+Y +G+ SLHHP YDFNDD++ G Sbjct: 317 GADKVVDLARPFMGSEDFAYMLKERPGSYFFLGSRVTGE-EKSLHHPGYDFNDDLLPIGA 375 Query: 381 RYWVALAQGYF 391 +W LA+ Y Sbjct: 376 AFWTELAEAYL 386 >AGc4953 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 393 Score = 348 bits (894), Expect = 9e-96, Method: Compositional matrix adjust. Identities = 173/382 (45%), Positives = 239/382 (62%), Gaps = 1/382 (0%) Query: 10 TLHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAGA 69 + H +RR LH HPE+ E +T+D + +L +GY VTRGL GTG+VA+L Sbjct: 10 SFANHMPDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNG 69 Query: 70 DPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLPG 129 D R + +RAD+DALPI E +AS GVMHACGHDGHT MLLGAA+++ + G Sbjct: 70 DSTRALGIRADIDALPIHEETGADYASTHQGVMHACGHDGHTAMLLGAAKIIAERRNFDG 129 Query: 130 SVHFVFQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRSGPIMAAGSR 189 ++H +FQP EE GAR MI+DGLFE+FP +AVF +HN PGLP GQF LR GPI+AA Sbjct: 130 TLHLIFQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFGQFVLRDGPILAAVDE 189 Query: 190 LKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDN 249 KIT+ G G H A+P DPI S+++ QT+ +R+ P AV++V FHAG N Sbjct: 190 CKITVRGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASN 249 Query: 250 VIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVDFFQYYPATINTPAET 309 VIP+ AE+ TIR+ ++ +L++ IR + A +YG V +D+ + Y AT+N AET Sbjct: 250 VIPETAEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAET 309 Query: 310 DFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHSLHHPHY 369 D+ + R+ G +++ +M +EDF +MLE+RPG Y +G A D LHHP + Sbjct: 310 DYVADLARRFAGPEKVLEMKRPSMGAEDFAYMLEKRPGCYFFLGTARTDN-DPPLHHPKF 368 Query: 370 DFNDDIIEAGVRYWVALAQGYF 391 DFND+I+ G +WV LA+ Y Sbjct: 369 DFNDEILPIGTTFWVDLAEDYL 390 >AGl2806 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 422 Score = 348 bits (893), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 173/371 (46%), Positives = 231/371 (62%), Gaps = 4/371 (1%) Query: 21 LRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAGADPQRGIVLRAD 80 +R DLH HPEL +E RTA ++ R L S GY VT G+GG GVVA+L ++ + +RAD Sbjct: 55 IRHDLHRHPELSLKETRTAALIARYLLSFGYEVTEGVGGHGVVATLKRGHGEKRLGIRAD 114 Query: 81 LDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLPGSVHFVFQPGEE 140 +DALPI E A+AS T G+MHACGHDGHT +LL AAR L + G++ +FQP EE Sbjct: 115 IDALPIHEETGLAYASETSGIMHACGHDGHTTVLLAAARYLAETSNFSGTLTLIFQPAEE 174 Query: 141 GGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRSGPIMAAGSRLKITITGKGAH 200 GAGAR MI D LFE+FP +AVFG+HNWPG+ GQFG GP MA+ + + I GKG H Sbjct: 175 IGAGARAMIKDRLFERFPVDAVFGLHNWPGVETGQFGFVEGPAMASVDKASVRIIGKGGH 234 Query: 201 AAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRAELRGT 260 A PH +DP+ + S + Q+I +R+ DP + AV++V H G NVIP+ EL+ T Sbjct: 235 GAAPHETVDPVLASASFITALQSIVSRNIDPREMAVLTVGSIHGGTASNVIPESVELQIT 294 Query: 261 IRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVDFFQYYPATINTPAETDFCERVIRQTF 320 R S+ ++ +L+E + + A AE++GA +V + + +P IN ET F V R+ F Sbjct: 295 ARAFSTAVRDRLEERLPALAKAQAESFGATADVSYSRGFPPVINHAPETAFARDVARRHF 354 Query: 321 GDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHSLHHPHYDFNDDIIEAGV 380 +I SEDF FML+ERPG+Y+ +GN S LH PHYDFND II Sbjct: 355 PAAQIADAFQPRTASEDFAFMLQERPGSYLFVGNG----ESAPLHSPHYDFNDAIIPPAA 410 Query: 381 RYWVALAQGYF 391 R+WV LA+ Y Sbjct: 411 RFWVRLAEAYL 421 >AGl2386 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 430 Score = 344 bits (883), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 177/380 (46%), Positives = 235/380 (61%), Gaps = 9/380 (2%) Query: 11 LHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGY-TVTRGLGGTGVVASLAGA 69 L Q + W R LH +PE+ ++ TA V +L+S G + GLG TGVV + G Sbjct: 53 LQQEVSEW---RHHLHENPEILYDVDNTAAFVAEKLKSFGVDEIVTGLGRTGVVGIIRGN 109 Query: 70 DP-QRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLP 128 P R I RAD+DALPI+E AS T G MHACGHDGHT MLLGAA+ L + Sbjct: 110 VPGNRTIGFRADMDALPILEETGKPWASKTAGAMHACGHDGHTAMLLGAAKYLTETRNFA 169 Query: 129 GSVHFVFQPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRSGPIMAAGS 188 GS+ +FQP EEGGAGA M++DGL E+F + V+GMHN PG+P G F +R G IMAA Sbjct: 170 GSIAVIFQPAEEGGAGALAMVEDGLMERFGIDEVYGMHNMPGIPLGAFAVRKGGIMAAPD 229 Query: 189 RLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTD 248 + IT+ G+G HAAQPH +DPI + +V Q IA+R+ DP+ V+SV FHAG + Sbjct: 230 KFSITVKGRGGHAAQPHRTIDPITIGAQIVGNLQMIASRNADPIRSVVVSVTRFHAGSSH 289 Query: 249 NVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVDFFQYYPATINTPAE 308 N+IP+ A + GT+R+L T++ QE I++M +A A GA+ EV + +Y P T N AE Sbjct: 290 NIIPNEALIAGTVRSLDETVRDLAQERIKQMAEGIALANGAEAEVVYERYCPVTFNHSAE 349 Query: 309 TDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHSLHHPH 368 TD+ RV R G+ + + +M EDF FML+ERPG ++ IGN S LH+P Sbjct: 350 TDYAVRVARDVVGEPNVDPDVDPSMAGEDFSFMLKERPGCFIFIGNGD----SAGLHNPS 405 Query: 369 YDFNDDIIEAGVRYWVALAQ 388 YDFND+ I G+ YWV LA+ Sbjct: 406 YDFNDEAIAYGISYWVKLAE 425