BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0058.p (345 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0948 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 486 e-137 AGc2601 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 414 e-115 SMa0512 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 409 e-114 AGl1538 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 404 e-112 SMb20445 [ER] COG1063 Threonine dehydrogenase and related Zn-dep... 351 1e-96 >RSp0948 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Rso Ralstonia solanacearum Length = 344 Score = 486 bits (1250), Expect = e-137, Method: Compositional matrix adjust. Identities = 241/340 (70%), Positives = 284/340 (83%) Query: 5 LVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHE 64 LVIHA +DLRI+ +DT +QP QL+V+V+ GGICGSDLHYY+HGGFG VR+KEPMVLGHE Sbjct: 4 LVIHAANDLRIEDIDTPEVQPDQLRVQVRFGGICGSDLHYYNHGGFGTVRVKEPMVLGHE 63 Query: 65 VSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHVQG 124 V+G++ E+G V A G R+AISPSRPCG C YCQ+G QNHCLDMR+YGSAMRTPHVQG Sbjct: 64 VAGRVEEIGPGVHRHAKGDRVAISPSRPCGQCHYCQQGLQNHCLDMRYYGSAMRTPHVQG 123 Query: 125 AFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIGAL 184 AFR+EI+I+ SQAH +AD +SD E AMAEPLSVALHAVRRAGPL+GKRVLVTGCGPIGAL Sbjct: 124 AFRREIVIDESQAHRLADTLSDQEGAMAEPLSVALHAVRRAGPLLGKRVLVTGCGPIGAL 183 Query: 185 VVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLFEA 244 +VVAARRAGA E++VTD+ + PL+AA+ VGAD IN+AE P LK Y DKG DVLFEA Sbjct: 184 IVVAARRAGAAEIIVTDMGAYPLKAAVAVGADVTINVAEQPDGLKPYTTDKGQIDVLFEA 243 Query: 245 SGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVELM 304 SGNERAL GA++ALRP+ IIVQVGLGG++ P N +V KELE RGAFRFHEEFAMAVEL+ Sbjct: 244 SGNERALRGAIDALRPRGIIVQVGLGGEMTLPLNAIVAKELELRGAFRFHEEFAMAVELL 303 Query: 305 NRKLVDVKPLITATYPIAQAVEAFEIAGDRSRMMKVQISF 344 N+ LVDVKPLI+AT P A AF +AGDR++ MKV + F Sbjct: 304 NKGLVDVKPLISATVPYRNAAHAFALAGDRTQAMKVLLDF 343 >AGc2601 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 371 Score = 414 bits (1064), Expect = e-115, Method: Compositional matrix adjust. Identities = 200/342 (58%), Positives = 258/342 (75%) Query: 3 QGLVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLG 62 +G+VIHA DLR++ + L +++V V GGICGSDLHYY+HGGFG VR++EPM LG Sbjct: 26 RGVVIHAAKDLRVEDVAGQPLAADEVRVAVAVGGICGSDLHYYNHGGFGTVRVREPMALG 85 Query: 63 HEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHV 122 HE +G + EVG++V+ + PG R+A++PS PCG C+YC +G+QN CLDMRF GSAMR+PHV Sbjct: 86 HEFAGTVVEVGSSVSHLVPGMRVAVNPSLPCGTCRYCAQGRQNQCLDMRFMGSAMRSPHV 145 Query: 123 QGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIG 182 QG FR+ + + +Q +AD +S GEAAMAEPL+V LHA R+AG L+GK VL+TG GPIG Sbjct: 146 QGGFREVVTVHSTQPVQIADGLSMGEAAMAEPLAVCLHAARQAGSLLGKTVLITGAGPIG 205 Query: 183 ALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLF 242 L ++ AR AGA +VVTDVA PL A ++GAD A+N+ L+ Y +KG FDVLF Sbjct: 206 MLSLLVARLAGAAHIVVTDVADAPLDLARRIGADEAVNILRDADMLEKYRFEKGVFDVLF 265 Query: 243 EASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVE 302 EASGN+ AL+ AL+ LRP IIVQ+GLGGD P NL+V KEL+ RG FRFHEEFA AV Sbjct: 266 EASGNQAALLPALDLLRPGGIIVQLGLGGDFTIPMNLIVAKELQLRGTFRFHEEFAQAVN 325 Query: 303 LMNRKLVDVKPLITATYPIAQAVEAFEIAGDRSRMMKVQISF 344 +M R L+DVKPLI+AT P QA EAF++AGDR++ MKVQ++F Sbjct: 326 MMGRGLIDVKPLISATLPFDQAREAFDLAGDRAKSMKVQLAF 367 >SMa0512 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Sme Sinorhizobium meliloti Length = 343 Score = 409 bits (1051), Expect = e-114, Method: Compositional matrix adjust. Identities = 195/342 (57%), Positives = 255/342 (74%), Gaps = 1/342 (0%) Query: 3 QGLVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLG 62 + +VIH DLR++ P ++++++ AGGICGSDLHYY+HGGFG VR+KEPM+LG Sbjct: 2 KAIVIHTAKDLRVEECAVEKPGPGEVEIRLAAGGICGSDLHYYNHGGFGTVRLKEPMILG 61 Query: 63 HEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHV 122 HEVSG +A +G V+D+A G +A+SPSRPCGAC YC +G NHC MRFYGSAM PH+ Sbjct: 62 HEVSGHVAALGEGVSDLAIGDLVAVSPSRPCGACDYCLKGLPNHCFHMRFYGSAMPFPHI 121 Query: 123 QGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIG 182 QGAFR+ ++ + SQ V A+ +S GEAAMAEPLSV LHA RRAG ++GKRVLVTGCGPIG Sbjct: 122 QGAFRERLVAKASQC-VKAEGLSAGEAAMAEPLSVTLHATRRAGEMLGKRVLVTGCGPIG 180 Query: 183 ALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLF 242 L ++AARRAGA E+V D++ L A VGAD +N++E L ++ +KGTFDVL+ Sbjct: 181 TLSILAARRAGAAEIVAADLSERALGFARAVGADRTVNLSEDRDGLVPFSENKGTFDVLY 240 Query: 243 EASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVE 302 E SG + ALV ++ALRP+ +IVQ+GLGGD+ P + KEL+ RG+FRFHEEFA AV+ Sbjct: 241 ECSGAQPALVAGIQALRPRGVIVQLGLGGDMALPMMAITAKELDLRGSFRFHEEFATAVK 300 Query: 303 LMNRKLVDVKPLITATYPIAQAVEAFEIAGDRSRMMKVQISF 344 LM L+DVKPLIT T P+ +A++AFEIA D+ + MK QI+F Sbjct: 301 LMQGGLIDVKPLITHTLPLGEALKAFEIASDKGQSMKTQIAF 342 >AGl1538 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 343 Score = 404 bits (1039), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/342 (57%), Positives = 247/342 (72%), Gaps = 1/342 (0%) Query: 3 QGLVIHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLG 62 + +V H D+RI+ P ++++++ GGICGSDLHYY+HGGFG VR++EPMVLG Sbjct: 2 KAIVAHGAKDVRIEDRPEEKPGPGEVRLRLARGGICGSDLHYYNHGGFGAVRLREPMVLG 61 Query: 63 HEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHV 122 HEVS I E+G V + G +A+SPSRPC C++CQEG N CL+MRFYGSAM PH+ Sbjct: 62 HEVSAVIEELGEGVEGLKIGGLVAVSPSRPCRTCRFCQEGLHNQCLNMRFYGSAMPFPHI 121 Query: 123 QGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIG 182 QGAFR EI++ + V AD +S GEAAMAEPL+V LHA RRAG L+GKRVLVTGCGPIG Sbjct: 122 QGAFR-EILVADALQCVPADGLSAGEAAMAEPLAVTLHATRRAGDLLGKRVLVTGCGPIG 180 Query: 183 ALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLF 242 L ++AARRAGA E+V TD++ L A + GAD IN + P AL AY A+KGTFD+L+ Sbjct: 181 ILSILAARRAGAAEIVATDLSDFTLGKAREAGADRVINSKDEPDALAAYGANKGTFDILY 240 Query: 243 EASGNERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVE 302 E SG AL G + ALRP+ IIVQ+GLGGD+ P + KEL+ RG+FRFHEEFA VE Sbjct: 241 ECSGAAVALAGGITALRPRGIIVQLGLGGDMSLPMMAITAKELDLRGSFRFHEEFATGVE 300 Query: 303 LMNRKLVDVKPLITATYPIAQAVEAFEIAGDRSRMMKVQISF 344 LM + L+DVKP IT T +A A+ AFE A DRSR MKVQI+F Sbjct: 301 LMRKGLIDVKPFITQTVDLADAISAFEFASDRSRAMKVQIAF 342 >SMb20445 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Sme Sinorhizobium meliloti Length = 352 Score = 351 bits (900), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 178/338 (52%), Positives = 233/338 (68%) Query: 7 IHAPHDLRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVS 66 ++ P DLR++ D A ++ +K+ AGGICGSDLHYY GGFG VR++EP++ GHE S Sbjct: 13 LYGPRDLRLEVADVPAPGDGEVLLKMAAGGICGSDLHYYQDGGFGPVRVREPIIPGHEAS 72 Query: 67 GQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPHVQGAF 126 G++A +G V+ +A +A++PS+PCG C++C EG HCLDMRF GSAMR PH QG F Sbjct: 73 GRVAVLGAGVSGLAIDDLVAVNPSQPCGTCRFCGEGLPVHCLDMRFLGSAMRLPHTQGMF 132 Query: 127 RQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPIGALVV 186 R +++ Q H VS GEAA AEPL+V LHA +AG + GK+VLVTG GPIGALVV Sbjct: 133 RDWLVVPAVQCHPAGSQVSPGEAACAEPLAVCLHAAAQAGEIRGKKVLVTGAGPIGALVV 192 Query: 187 VAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLFEASG 246 AAR AGA E+VVTD+A L A +GA +N+ PA L AY ADKG F V FE S Sbjct: 193 AAARHAGADEIVVTDLADAALDRAKAMGATRIVNVHRDPAGLAAYEADKGKFHVAFECSA 252 Query: 247 NERALVGALEALRPQAIIVQVGLGGDIQFPANLLVGKELEWRGAFRFHEEFAMAVELMNR 306 E AL GA+ A+RP+ IVQVG+ G+I P + LVGKEL G+ RF EFA+AV L++ Sbjct: 253 AEPALRGAVAAVRPRGTIVQVGVTGEITLPLHALVGKELRLVGSQRFDGEFALAVALISD 312 Query: 307 KLVDVKPLITATYPIAQAVEAFEIAGDRSRMMKVQISF 344 + +DV+P+I+ +P+ +AV AFE AGDRS KVQ++F Sbjct: 313 RRIDVRPIISHQFPVDEAVRAFEQAGDRSAACKVQLTF 350