BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0035.p (206 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20005 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium... 275 4e-74 PA3035 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aer... 170 2e-42 SMa2115 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium ... 117 1e-26 SMa1497 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium ... 112 4e-25 alr3195 [O] COG0625 Glutathione S-transferase Nos Nostoc sp. PCC... 99 8e-21 >SMb20005 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 203 Score = 275 bits (704), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 138/203 (67%), Positives = 161/203 (79%) Query: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHKQPVFLALNPFGQVPVLEDGEV 60 MK++HHPLSGHAHRA LF SLLG+ ELV VD A HKQ FL LN FGQVPVL+D Sbjct: 1 MKLYHHPLSGHAHRARLFLSLLGIEHELVVVDFARREHKQADFLKLNSFGQVPVLDDAGT 60 Query: 61 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAF 120 +I+DSNAILVYLAKK GR DWLPEDA GAAAVQRWLSVAAG++A+GPA ARL+ VF A + Sbjct: 61 IISDSNAILVYLAKKTGRPDWLPEDAVGAAAVQRWLSVAAGQIAHGPAQARLINVFKAPY 120 Query: 121 NAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQG 180 EEVI R+H +L +EQ L R W+AAD PTIADVA+YSY+ARAPEG+VDL+ YP ++ Sbjct: 121 RPEEVIPRSHAILALIEQELEGRGWIAADRPTIADVALYSYVARAPEGDVDLQPYPEIRA 180 Query: 181 FLQRIEALPGFVPFAQTPAGLMA 203 +L RIEALPGFV F +TP GL A Sbjct: 181 WLARIEALPGFVEFEKTPVGLAA 203 >PA3035 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aeruginosa Length = 198 Score = 170 bits (431), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 90/195 (46%), Positives = 123/195 (63%), Gaps = 1/195 (0%) Query: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHKQPVFLALNPFGQVPVLEDGEV 60 MK+H LSG+A++ LF SL+G A L VD HK FL +NP GQ+PVLEDGE+ Sbjct: 1 MKLHDLALSGNAYKVRLFLSLIGREATLRPVDFLNREHKTQAFLRMNPRGQIPVLEDGEL 60 Query: 61 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAF 120 + DS+AILVYLA++ W P D + WLS +A E+ GPA+ARL+T F Sbjct: 61 QLGDSHAILVYLARRHADPVWYPLDPVSQGRIAYWLSYSANEVQNGPASARLITRFNLPL 120 Query: 121 NAEEVIQRAHLLLGRLEQHLAKRDWLA-ADHPTIADVAVYSYLARAPEGNVDLRGYPGVQ 179 + E+ QR +L +++HLA+ WLA + P+IADVA+Y YLA APEG + L YP + Sbjct: 121 DREQTHQRGLEVLRLVDRHLAEHRWLAQGEQPSIADVALYPYLALAPEGGLALDHYPALN 180 Query: 180 GFLQRIEALPGFVPF 194 ++ RI ALPG++P Sbjct: 181 AWIARIRALPGYIPM 195 >SMa2115 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 215 Score = 117 bits (294), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 72/210 (34%), Positives = 115/210 (54%), Gaps = 11/210 (5%) Query: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAG-AHKQPVFLALNPFGQVPVLEDGE 59 +K++ + LSG+ ++ L S+LG+ + V VD G HK FL NP GQ+PV++D Sbjct: 2 IKLYDYELSGNCYKLRLLMSILGVDYKTVPVDFYPGREHKSEWFLKFNPLGQLPVIDDDG 61 Query: 60 VVIADSNAILVYLAKKAGRS-DWLPEDA-QGAAAVQRWLSVAAGELAYGPAAARLVTVFG 117 +V+ D+ AILVYLA K + W P D + + +WL+ A + +AARL Sbjct: 62 LVLRDAQAILVYLAAKYDTAGSWYPRDNPELLGEISQWLAF-ADSITSTASAARLHDALF 120 Query: 118 AAFNAEEVIQRAHLLLGRLEQHL-----AKRDWL-AADHPTIADVAVYSYLARAPEGNVD 171 F+ E AH L L++HL +R W+ +A HPTIAD+A + Y+ + EG + Sbjct: 121 YDFDIETARAGAHRLFRILDEHLWFGERQERQWICSAKHPTIADIACFPYIMLSEEGGIS 180 Query: 172 LRGYPGVQGFLQRIEALPGFVPFAQT-PAG 200 + YP ++ + R++ + GF+ + PAG Sbjct: 181 RQDYPAIRRWCDRVKRINGFIVMSGVFPAG 210 >SMa1497 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 215 Score = 112 bits (281), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 72/210 (34%), Positives = 112/210 (53%), Gaps = 11/210 (5%) Query: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAG-AHKQPVFLALNPFGQVPVLEDGE 59 + ++ + LSG+ ++ L S+LG+ + V VD G HK FL LNP GQ+PV+ED Sbjct: 2 ITLYDYELSGNCYKLRLLMSMLGIEYKTVPVDFYPGREHKSDWFLKLNPLGQLPVIEDDG 61 Query: 60 VVIADSNAILVYLAKKAGRS-DWLPEDAQG-AAAVQRWLSVAAGELAYGPAAARLVTVFG 117 +V+ D+ AILVY+ K S W P D + +WL+ A G + +AARL Sbjct: 62 LVLRDAQAILVYIGSKYDPSGTWYPRDNPALLGEISQWLAFADG-ITGTASAARLHDGLF 120 Query: 118 AAFNAEEVIQRAHLLLGRLEQHL-----AKRDWL-AADHPTIADVAVYSYLARAPEGNVD 171 + E AH L L++HL RDW+ +A HPT+AD+A + Y+ + EG + Sbjct: 121 YELDVEAARAGAHRLFRILDEHLWFGEQEGRDWICSAPHPTVADIACFPYIMLSEEGGIP 180 Query: 172 LRGYPGVQGFLQRIEALPGFVPFAQT-PAG 200 + YP ++ + R++ + GF + PAG Sbjct: 181 RQDYPAIRRWCDRLKRIKGFTVMSGIFPAG 210 >alr3195 [O] COG0625 Glutathione S-transferase Nos Nostoc sp. PCC 7120 Length = 245 Score = 98.6 bits (244), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 61/196 (31%), Positives = 103/196 (52%), Gaps = 9/196 (4%) Query: 9 SGHAHRAVLFASLLGLPAELVEVDLAAGAHKQPVFLALNPFGQVPVLEDGE-VVIADSNA 67 SG+ ++ L + +G+P E EV++ G + P FL+ N G++PVLE +A+SNA Sbjct: 47 SGNGYKVRLLLTQMGIPFERTEVNILKGESRTPEFLSKNSNGRIPVLEVAPGKYLAESNA 106 Query: 68 ILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARL-VTVFGAAFNA---- 122 IL+YL++ +++LP D A V +WL A +R V++ G Sbjct: 107 ILLYLSEY---TEFLPYDRYLKAQVMQWLFFEQYSHEPYIATSRFWVSILGKVEEYRTAL 163 Query: 123 EEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQGFL 182 EE + + L +E HL ++ + TIAD+A+++Y A EG DL +P +Q ++ Sbjct: 164 EEKREPGYAALKVMENHLKCHNFFVDERYTIADIALFAYTHVADEGGFDLTPFPAIQAWI 223 Query: 183 QRIEALPGFVPFAQTP 198 R++A PG++ P Sbjct: 224 DRVKAQPGYINITHEP 239