BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0029.p (185 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl1073 [IQR] COG1028 Dehydrogenases with different specificitie... 269 2e-72 SMc01991 [IQR] COG1028 Dehydrogenases with different specificiti... 236 2e-62 mll4054 [IQR] COG1028 Dehydrogenases with different specificitie... 114 1e-25 YPO3351 [IQR] COG1028 Dehydrogenases with different specificitie... 112 5e-25 kduD [IQR] COG1028 Dehydrogenases with different specificities (... 110 2e-24 >AGl1073 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 270 Score = 269 bits (688), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 134/181 (74%), Positives = 151/181 (83%), Gaps = 11/181 (6%) Query: 5 GTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMVNI 64 G DFL+NSAGINVLESVL+MSD GYEAVLG+NLRAAL+TCQ FAR R+A GGGGA+VNI Sbjct: 101 GPCDFLINSAGINVLESVLDMSDAGYEAVLGINLRAALVTCQEFARGRVANGGGGAIVNI 160 Query: 65 TSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWSDP 124 TSIAGHRGF+ HLCYAASKAGLEGATRVLA+ELGPHGIRVNAVAPTITLTELA AW+DP Sbjct: 161 TSIAGHRGFQDHLCYAASKAGLEGATRVLAKELGPHGIRVNAVAPTITLTELAAEAWNDP 220 Query: 125 AKSGPIRARFASPEDVAXARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGFLA 184 KS P+ RHP RFA E+VA+SIA+LLS D+ M++GAV+PVDGGFLA Sbjct: 221 EKSQPM-----------MVRHPLNRFAEAEEVAQSIALLLSDDSKMISGAVLPVDGGFLA 269 Query: 185 V 185 V Sbjct: 270 V 270 >SMc01991 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 241 Score = 236 bits (602), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 125/185 (67%), Positives = 150/185 (81%), Gaps = 11/185 (5%) Query: 1 MAEAGTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGA 60 M EAGT D LVN AG NVLES+++M+D+GY+ V+ NLRAALI Q FAR R+A GGGGA Sbjct: 68 MVEAGTCDILVNCAGTNVLESLIDMTDDGYDVVMNTNLRAALICAQEFARARMAAGGGGA 127 Query: 61 MVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAA 120 +VN+TSIAGHRGF+ H+CYAASKAGLEGATRV+A+ELGP+GIRV A+APTIT+TELA AA Sbjct: 128 IVNVTSIAGHRGFQNHVCYAASKAGLEGATRVMAKELGPYGIRVVAIAPTITMTELAAAA 187 Query: 121 WSDPAKSGPIRARFASPEDVAXARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDG 180 W+DP KS P+ RHP+ RFA+ +DVARSIAMLLS DA M++GAV+PVDG Sbjct: 188 WADPQKSEPM-----------MVRHPAGRFATVDDVARSIAMLLSSDAEMVSGAVLPVDG 236 Query: 181 GFLAV 185 GFLAV Sbjct: 237 GFLAV 241 >mll4054 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 258 Score = 114 bits (284), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 67/182 (36%), Positives = 100/182 (54%), Gaps = 11/182 (6%) Query: 3 EAGTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMV 62 E+G D LVN+AGI +++++ ++ V+ VNL+ + CQ+FAR +A G G +V Sbjct: 87 ESGPLDGLVNNAGIIRRADAVDLTETDWDDVMDVNLKTVFLLCQSFARRALADGRRGKIV 146 Query: 63 NITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWS 122 NI S+ +G + Y ASK G+ G TR+LA E GI VNA+AP T +A + Sbjct: 147 NIASVLSFQGGIRVASYTASKHGVLGITRLLACEWAAKGINVNAIAPGYVETNNTQALRA 206 Query: 123 DPAKSGPIRARFASPEDVAXARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGGF 182 DP +S I R P+ R+ P D+ + LL+ + ++GA VPVDGG+ Sbjct: 207 DPDRSAAILGRI-----------PAGRWGEPSDIGDAAVFLLARASDYMHGAAVPVDGGW 255 Query: 183 LA 184 LA Sbjct: 256 LA 257 >YPO3351 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Ype Yersinia pestis Length = 256 Score = 112 bits (280), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 70/179 (39%), Positives = 103/179 (57%), Gaps = 15/179 (8%) Query: 5 GTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGAMVNI 64 G D LVNSAGI L+ ++ ++ ++ + +NL+ + CQ + I Q G G +VN+ Sbjct: 89 GQLDILVNSAGIAALDPAEKVREQDWDRTIDINLKGVFLMCQEVGKHFI-QHGHGKIVNL 147 Query: 65 TSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAAWSDP 124 S AG HL Y ASK G+ G T+VLA E GP I+VNA++PT+ LT L + AW Sbjct: 148 ASQAGVVALPNHLAYCASKFGVIGITKVLALEWGPLDIQVNAISPTVVLTALGQKAW--- 204 Query: 125 AKSGPIRARFASPEDVAXARHPSARFASPEDVARSIAMLLSPDAA-MLNGAVVPVDGGF 182 SG + ED+ + P+ RFA P +VA + A+ L+ DAA M+ G + +DGG+ Sbjct: 205 --SGQL------AEDMKL-KIPARRFAYPAEVA-ACALFLASDAANMITGENLVIDGGY 253 >kduD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Eco Escherichia coli K12 Length = 253 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 65/184 (35%), Positives = 98/184 (53%), Gaps = 11/184 (5%) Query: 1 MAEAGTADFLVNSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIAQGGGGA 60 +AE G D LVN+AG+ E LE S++ ++ V+ +N+++ QA A+ IAQG GG Sbjct: 80 VAEFGHIDILVNNAGLIRREDALEFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGK 139 Query: 61 MVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLTELAEAA 120 ++NI S+ +G + Y ASK+G+ G TR++A E H I VNA+AP T + Sbjct: 140 IINIASMLSFQGGIRVPSYTASKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQL 199 Query: 121 WSDPAKSGPIRARFASPEDVAXARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDG 180 +D +S I R P+ R+ P D+ I L S + +NG + VDG Sbjct: 200 RADEQRSAEILDRI-----------PAGRWGLPSDLMGPIVFLASSASDYVNGYTIAVDG 248 Query: 181 GFLA 184 G+LA Sbjct: 249 GWLA 252