BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0330.p (219 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0614 [C] COG0778 Nitroreductase Rso Ralstonia solanacearum 299 4e-81 PA1274 [C] COG0778 Nitroreductase Pae Pseudomonas aeruginosa 286 2e-77 Rv0306 [C] COG0778 Nitroreductase Mtu Mycobacterium tuberculosis... 249 4e-66 MT0319 [C] COG0778 Nitroreductase MtC Mycobacterium tuberculosis... 249 4e-66 SMc00166 [C] COG0778 Nitroreductase Sme Sinorhizobium meliloti 162 4e-40 >RSp0614 [C] COG0778 Nitroreductase Rso Ralstonia solanacearum Length = 216 Score = 299 bits (765), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 148/211 (70%), Positives = 183/211 (86%), Gaps = 1/211 (0%) Query: 2 NPHRYAQPEIEAIYRVIRERRDMRHFLPDAVAPELLERLLQAAHHAPSVGFMQPWRFLRI 61 HRY + ++ A+YRVIRERRDMRHF PD + P LL RLL+AAH APSVG+MQPWRF+RI Sbjct: 3 TTHRYTEADVAAVYRVIRERRDMRHFRPDPIEPALLARLLRAAHLAPSVGYMQPWRFIRI 62 Query: 62 R-SXLRRQIHALVEQERIATAQALGQREQEFMRLKVEGLLECGEVLVAALMDQRERHVFG 120 + LR +IHALVE+ER++TA+ALGQRE EF+RLKVEG+L+CGEVLVAALMD+RE H+FG Sbjct: 63 TDAALRARIHALVEEERLSTARALGQREDEFLRLKVEGILDCGEVLVAALMDKREPHIFG 122 Query: 121 RRTMPQMDLASLSCALQNMWLAARAEGLGMGWVSLFDPAALAQLLRMPDGAAPVAVLCLG 180 RRT+P+MDLAS++CA+QN+WLAARAEG+G+GWVS+FDP L QLL +P GA PVAVLCLG Sbjct: 123 RRTLPEMDLASVACAIQNLWLAARAEGIGVGWVSMFDPQQLGQLLHLPAGARPVAVLCLG 182 Query: 181 HVPAFYDKPMLEQEQWAARQPLADLVFDDHW 211 HV AFYD+PMLEQE WA+RQPL ++F++ W Sbjct: 183 HVDAFYDRPMLEQEHWASRQPLESMLFENRW 213 >PA1274 [C] COG0778 Nitroreductase Pae Pseudomonas aeruginosa Length = 218 Score = 286 bits (733), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 142/211 (67%), Positives = 168/211 (79%), Gaps = 1/211 (0%) Query: 2 NPHRYAQPEIEAIYRVIRERRDMRHFLPDAVAPELLERLLQAAHHAPSVGFMQPWRFLRI 61 + H ++ E A+YR I ERRDMRHF +VAPELL RLL+AAH APSVG MQPWRF+RI Sbjct: 4 SSHAFSATERAAVYRAIAERRDMRHFSGGSVAPELLARLLEAAHQAPSVGLMQPWRFIRI 63 Query: 62 RS-XLRRQIHALVEQERIATAQALGQREQEFMRLKVEGLLECGEVLVAALMDQRERHVFG 120 LRR IH LVE+ER TA+ALG+R EFMRLKVEG+L+C E+LVAALMD RERHVFG Sbjct: 64 TEPALRRAIHGLVEEERGRTAEALGERSDEFMRLKVEGVLDCAELLVAALMDDRERHVFG 123 Query: 121 RRTMPQMDLASLSCALQNMWLAARAEGLGMGWVSLFDPAALAQLLRMPDGAAPVAVLCLG 180 RRT+PQMDLASL+CA+QN+WLA+RAEGLGMGWVSLFDPAAL +LL +P G+ VAVLCLG Sbjct: 124 RRTLPQMDLASLACAIQNLWLASRAEGLGMGWVSLFDPAALGELLGLPPGSEAVAVLCLG 183 Query: 181 HVPAFYDKPMLEQEQWAARQPLADLVFDDHW 211 V FY PML E W+ +PLA+LV+ + W Sbjct: 184 PVAEFYPAPMLALEGWSEPRPLAELVYQNRW 214 >Rv0306 [C] COG0778 Nitroreductase Mtu Mycobacterium tuberculosis H37Rv Length = 223 Score = 249 bits (635), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 132/208 (63%), Positives = 163/208 (78%), Gaps = 2/208 (0%) Query: 6 YAQPEIEAIYRVIRERRDMRHFLPDAVAPE-LLERLLQAAHHAPSVGFMQPWRFLRIRS- 63 ++ PE A+YRVI ERRDMR F+P V E +L RLL AAH APSVG MQPWRF+RI Sbjct: 2 FSAPERRAVYRVIAERRDMRRFVPGGVVSEDVLARLLHAAHAAPSVGLMQPWRFIRITDE 61 Query: 64 XLRRQIHALVEQERIATAQALGQREQEFMRLKVEGLLECGEVLVAALMDQRERHVFGRRT 123 L+R+IHALV+ ER+ TA+ALG RE+EF+ LKVEG+L+C E+LV AL D+R ++FGRRT Sbjct: 62 TLKRRIHALVDDERLLTAEALGAREEEFLALKVEGILDCAELLVVALCDRRGSYIFGRRT 121 Query: 124 MPQMDLASLSCALQNMWLAARAEGLGMGWVSLFDPAALAQLLRMPDGAAPVAVLCLGHVP 183 +PQMDLAS+SCA+QN+WLAAR+EGLGMGWVSLFDP LA LL MP A PVA+LCLG VP Sbjct: 122 LPQMDLASVSCAIQNLWLAARSEGLGMGWVSLFDPQRLAALLAMPADAEPVAILCLGPVP 181 Query: 184 AFYDKPMLEQEQWAARQPLADLVFDDHW 211 F D+P LE + WA +PLA+ V ++ W Sbjct: 182 EFPDRPALELDGWAYARPLAEFVSENRW 209 >MT0319 [C] COG0778 Nitroreductase MtC Mycobacterium tuberculosis CDC1551 Length = 223 Score = 249 bits (635), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 132/208 (63%), Positives = 163/208 (78%), Gaps = 2/208 (0%) Query: 6 YAQPEIEAIYRVIRERRDMRHFLPDAVAPE-LLERLLQAAHHAPSVGFMQPWRFLRIRS- 63 ++ PE A+YRVI ERRDMR F+P V E +L RLL AAH APSVG MQPWRF+RI Sbjct: 2 FSAPERRAVYRVIAERRDMRRFVPGGVVSEDVLARLLHAAHAAPSVGLMQPWRFIRITDE 61 Query: 64 XLRRQIHALVEQERIATAQALGQREQEFMRLKVEGLLECGEVLVAALMDQRERHVFGRRT 123 L+R+IHALV+ ER+ TA+ALG RE+EF+ LKVEG+L+C E+LV AL D+R ++FGRRT Sbjct: 62 TLKRRIHALVDDERLLTAEALGAREEEFLALKVEGILDCAELLVVALCDRRGSYIFGRRT 121 Query: 124 MPQMDLASLSCALQNMWLAARAEGLGMGWVSLFDPAALAQLLRMPDGAAPVAVLCLGHVP 183 +PQMDLAS+SCA+QN+WLAAR+EGLGMGWVSLFDP LA LL MP A PVA+LCLG VP Sbjct: 122 LPQMDLASVSCAIQNLWLAARSEGLGMGWVSLFDPQRLAALLAMPADAEPVAILCLGPVP 181 Query: 184 AFYDKPMLEQEQWAARQPLADLVFDDHW 211 F D+P LE + WA +PLA+ V ++ W Sbjct: 182 EFPDRPALELDGWAYARPLAEFVSENRW 209 >SMc00166 [C] COG0778 Nitroreductase Sme Sinorhizobium meliloti Length = 227 Score = 162 bits (411), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 90/210 (42%), Positives = 127/210 (60%), Gaps = 4/210 (1%) Query: 6 YAQPEIEAIYRVIRERRDMR-HFLPDAVAPELLERLLQAAHHAPSVGFMQPWRFLRIR-S 63 ++ E A+YR I RRD+R FLP+ ++ EL+ RLL AAH APSVGFMQPW F+ +R Sbjct: 15 FSSDERAAVYRAIETRRDVRDEFLPEPLSEELIARLLGAAHQAPSVGFMQPWNFVLVRQD 74 Query: 64 XLRRQIHALVEQERIATAQAL-GQREQEFMRLKVEGLLECG-EVLVAALMDQRERHVFGR 121 R ++ ++ A+ G+R+ ++ LK+EG+ + + V + V GR Sbjct: 75 ETREKVWQAFQRANDEAAEMFSGERQAKYRSLKLEGIRKAPLSICVTCDRTRGGAVVLGR 134 Query: 122 RTMPQMDLASLSCALQNMWLAARAEGLGMGWVSLFDPAALAQLLRMPDGAAPVAVLCLGH 181 PQMDL S CA+QN+WLAARAEG+G+GWVS+F + + +L +PD VA LCLG Sbjct: 135 THNPQMDLYSTVCAVQNLWLAARAEGVGVGWVSIFHESEIKAILGIPDHVEIVAWLCLGF 194 Query: 182 VPAFYDKPMLEQEQWAARQPLADLVFDDHW 211 V Y +P L + W R PL DLVF++ W Sbjct: 195 VDRLYQEPELAAKGWRQRLPLEDLVFEEGW 224