BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0229.p (257 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1730 [IQR] COG1028 Dehydrogenases with different specificitie... 317 2e-86 RSc2597 [IQR] COG1028 Dehydrogenases with different specificitie... 273 4e-73 AGl3471 [IQR] COG1028 Dehydrogenases with different specificitie... 169 6e-42 BH2491 [IQR] COG1028 Dehydrogenases with different specificities... 151 1e-36 mlr7057 [IQR] COG1028 Dehydrogenases with different specificitie... 150 2e-36 >RSc1730 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 256 Score = 317 bits (811), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 168/257 (65%), Positives = 189/257 (73%), Gaps = 1/257 (0%) Query: 1 MNTPTQSPRIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVA 60 M P IAL+TGGSRGLG+N LAR G VI TY NQ A+A VAEL ALG A Sbjct: 1 MTQPQSPTPIALVTGGSRGLGRNMVQKLARGGTDVIFTYRSNQAEAQALVAELAALGRRA 60 Query: 61 VALRLDLADSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDELF 120 AL+LD A+F AFA AVR L WQ E LVNN G+G+HAS+ E +EA FD L Sbjct: 61 AALQLDAGQIATFAAFADAVRGVL-AQWQRERIDALVNNAGIGIHASLAEMTEAQFDALM 119 Query: 121 KVHLKGPFFLTQALLPLLAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEF 180 VH KG FFLTQALLPL+A GGRI+NISSGL RF+ GY AYA+MKG VEV+T+YMAKE Sbjct: 120 NVHFKGVFFLTQALLPLIADGGRIVNISSGLTRFAFVGYGAYAAMKGAVEVLTKYMAKEL 179 Query: 181 GARGIVVNTVAPGAIETDFGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIAALLRPD 240 G RGI VN VAPGAIETDFGGGRVRD+A LN+ VA QTALGR GLPDDIG +A+LL PD Sbjct: 180 GPRGIAVNVVAPGAIETDFGGGRVRDDANLNAMVAAQTALGRVGLPDDIGGVVASLLAPD 239 Query: 241 NGWINAQRVEVSGGMFI 257 N WINAQR+E SGGMF+ Sbjct: 240 NRWINAQRIEASGGMFL 256 >RSc2597 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 252 Score = 273 bits (697), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 131/251 (52%), Positives = 172/251 (68%) Query: 7 SPRIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAVALRLD 66 + +IALITG SRGLG+N ALHLA+ G +I TY + A+ E++ALG A L LD Sbjct: 2 TTKIALITGASRGLGRNMALHLAKRGVHIIGTYRSGAEQAQTLKQEIEALGGKATMLALD 61 Query: 67 LADSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDELFKVHLKG 126 + ++A +PAF AV + LK + E F +L+NN G G+ A + +E F L HL+G Sbjct: 62 VTETAGYPAFTSAVTDTLKNDFGRERFDFLINNAGNGLFAKFVDATEEQFASLIATHLRG 121 Query: 127 PFFLTQALLPLLAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFGARGIV 186 P FLTQ LLPLL GGRI+N+SSG RF++PGY+ YA++K +EV+TRYMA E G+R I Sbjct: 122 PIFLTQKLLPLLENGGRILNVSSGFVRFTMPGYSVYAAVKAALEVLTRYMAVELGSRQIR 181 Query: 187 VNTVAPGAIETDFGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIAALLRPDNGWINA 246 VN +APGAI TDFGGG VRDN ++N++VA ALGR GLPDDIG A+AA+L D GW N Sbjct: 182 VNAIAPGAIATDFGGGAVRDNKDVNAYVAQGIALGRVGLPDDIGGAVAAILSDDMGWANG 241 Query: 247 QRVEVSGGMFI 257 ++SGG + Sbjct: 242 TTFDISGGQLL 252 >AGl3471 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 257 Score = 169 bits (428), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 100/253 (39%), Positives = 141/253 (55%), Gaps = 3/253 (1%) Query: 7 SPRIALITGGSRGLGKNTALHLARDGAGVI-LTYVGNQQAAEATVAELQALGAVAVALRL 65 S ++ALITG SRG+G+ AL A++GAG+I + Y N AA+AT+ E++ALG+ A+AL+ Sbjct: 6 SNKVALITGSSRGIGRAAALAFAKEGAGLIGVHYGANPDAAQATIREIEALGSKAIALKA 65 Query: 66 DLAD-SASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDELFKVHL 124 DL D A+ ++A LVNN G+ +++ +EA FDEL ++ Sbjct: 66 DLRDGKAATDNLWDQFKQASIAFTGTPSLDILVNNAGIAPATPLKDMTEAAFDELMTINF 125 Query: 125 KGPFFLTQALLPLLAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFGARG 184 K PFFL QA+ L GRIINIS+G R + P + AYA+ KG +E +T +A EFG RG Sbjct: 126 KAPFFLIQAVADHLRDNGRIINISTGFTRIAAPTHPAYAASKGALETLTLALAPEFGRRG 185 Query: 185 IVVNTVAPGAIETDFGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIAALLRPDNGWI 244 I VN+V PG ETD G + E + + R G +DI I L D W Sbjct: 186 ITVNSVLPGVTETDMNAGWLA-APEARAGAEALSVFSRIGRAEDIADVITFLASDDARWT 244 Query: 245 NAQRVEVSGGMFI 257 Q ++ +GG I Sbjct: 245 TGQMIDATGGARI 257 >BH2491 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 246 Score = 151 bits (382), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 93/251 (37%), Positives = 140/251 (55%), Gaps = 11/251 (4%) Query: 9 RIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAVALRLDLA 68 + A++TG SRG+G+ TA+ LAR GA V++ Y GN++ AE VAE++ LG A+A++ D+A Sbjct: 5 KTAIVTGASRGIGRATAMELARHGANVVVNYAGNKEKAEKVVAEIKELGVEAIAIQADVA 64 Query: 69 DSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDELFKVHLKGPF 128 DS S A V+E + T + LVNN G+ E +D + +LKG F Sbjct: 65 DSESVQAM---VKETIDTFGAVD---ILVNNAGITRDNLFMRMKEEDWDAVIDTNLKGVF 118 Query: 129 FLTQALL-PLLAQG-GRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFGARGIV 186 ++A+ P++ Q GRIIN+SS + G A Y + K GV +T+ +A+E R I Sbjct: 119 HCSKAVTRPMMKQRFGRIINVSSVVGAIGNAGQANYVAAKAGVIGLTKTLARELANRNIT 178 Query: 187 VNTVAPGAIETDFGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIAALLRPDNGWINA 246 VN VAPG IETD G D + + + Q L R G P+++ A+ L D ++ Sbjct: 179 VNAVAPGFIETDMTGELPED---VKAQMLGQIPLARLGQPEEVAKAVRFLASDDASYLTG 235 Query: 247 QRVEVSGGMFI 257 Q + V+GGM + Sbjct: 236 QTIHVNGGMVM 246 >mlr7057 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 248 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 95/248 (38%), Positives = 131/248 (52%), Gaps = 7/248 (2%) Query: 7 SPRIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAVALRLD 66 S + AL+TG SRG+G++ AL LAR GA V++ Y + AEA VAE++ G A +L D Sbjct: 5 SGKTALVTGASRGMGRSAALALARMGAQVLIHYSTGLKEAEAVVAEIREAGGRADSLAAD 64 Query: 67 LADSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDELFKVHLKG 126 L+ A+ VR + + LV N G A+IE+T+ FD LF V+++ Sbjct: 65 LSAPDGAHKLAKQVRSVI-----GDRLDILVANAGTSKSATIEQTTVEDFDRLFAVNVRA 119 Query: 127 PFFLTQALLPLLAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFGARGIV 186 PFFL Q LLP++ + I+ ISS A ++ AYA+ KG ++ + ++ A G RGI Sbjct: 120 PFFLVQQLLPIMGKDSSIVLISSLAAHAAVGTLPAYAATKGAIDTLVKHFAAVLGERGIR 179 Query: 187 VNTVAPGAIETDFGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIAALLRPDNGWINA 246 VN VAPG + TD D F AL R PDDIG +A L D WI+ Sbjct: 180 VNAVAPGVVGTDMSSFTKTDAGR--DFTLGIQALKRLAQPDDIGGVVAFLATDDARWISG 237 Query: 247 QRVEVSGG 254 V V GG Sbjct: 238 DIVRVDGG 245