BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0222.p (260 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0745 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Pae P... 369 e-102 RSp0648 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Rso ... 287 2e-77 AF0435 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Afu A... 183 3e-46 CAC2712 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Cac ... 168 9e-42 BH0207 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Bha B... 167 3e-41 >PA0745 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Pae Pseudomonas aeruginosa Length = 272 Score = 369 bits (947), Expect = e-102, Method: Compositional matrix adjust. Identities = 185/253 (73%), Positives = 207/253 (81%) Query: 7 LKLEKTGHTAVLTLSNPPANTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSAGA 66 L +EK GHTA++T+++PPANTW R +L L +L++ LN D DIY LVVTG+G KFFSAGA Sbjct: 19 LTVEKHGHTALITINHPPANTWDRDSLIGLRQLIEHLNRDDDIYALVVTGQGPKFFSAGA 78 Query: 67 DLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEE 126 DL +FADGDKA AREMARRFGEAFE L FRGVSIAAINGYAMGGGLECALACDIRIAE Sbjct: 79 DLNMFADGDKARAREMARRFGEAFEALRDFRGVSIAAINGYAMGGGLECALACDIRIAER 138 Query: 127 QAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVVPRG 186 QAQMALPEAAVGLLPCAGGTQ LPWLVGEGWAKRMILC ERV+AETALRIGLVE+VV G Sbjct: 139 QAQMALPEAAVGLLPCAGGTQALPWLVGEGWAKRMILCNERVDAETALRIGLVEQVVDSG 198 Query: 187 QARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQDQQEGV 246 +AR AL LA + +QSP ++ K LIQGAR T L ERE FVDLFD QD +EGV Sbjct: 199 EARGAALLLAAKVARQSPVAIRTIKPLIQGARERAPNTWLPEERERFVDLFDAQDTREGV 258 Query: 247 QAFLEKRSPQWKN 259 AFLEKR P+W+N Sbjct: 259 NAFLEKRDPKWRN 271 >RSp0648 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Rso Ralstonia solanacearum Length = 266 Score = 287 bits (734), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 159/248 (64%), Positives = 179/248 (72%), Gaps = 1/248 (0%) Query: 13 GHTAVLTLSNPPANTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSAGADLKLFA 72 G A+LTLS PPAN +T L L V+ L+AD + LV+TG+G KFFSAGADL FA Sbjct: 10 GTVALLTLSRPPANAFTPEGLRQLQATVETLDADPRVRALVITGDGPKFFSAGADLNTFA 69 Query: 73 DGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMAL 132 GD+ +ARE A RFG AFE L R V IAAINGYAMGGGLECALACDIRIAE AQ+A+ Sbjct: 70 SGDRDVAREAAARFGAAFEALQNARPVVIAAINGYAMGGGLECALACDIRIAERHAQLAV 129 Query: 133 PEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERA 192 PE AVGLL C GTQ LPWLVGEGWAKRMIL GER++A+TALRIGLVEEVV G ARE A Sbjct: 130 PETAVGLLCCGCGTQTLPWLVGEGWAKRMILTGERIDAQTALRIGLVEEVVDTGAAREAA 189 Query: 193 LALAQQAEKQSPSSVAASKQLIQGARHN-PLGTILQTERELFVDLFDTQDQQEGVQAFLE 251 LA+A++ SP +V SK LI ARH P L ERE FVDLF DQ+EGV AFLE Sbjct: 190 LAMARRVTTLSPRAVTFSKALIHQARHGVPRTAALAVERERFVDLFGHPDQREGVNAFLE 249 Query: 252 KRSPQWKN 259 KR P+W Sbjct: 250 KRPPRWHT 257 >AF0435 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Afu Archaeoglobus fulgidus Length = 256 Score = 183 bits (465), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 107/258 (41%), Positives = 157/258 (60%), Gaps = 10/258 (3%) Query: 7 LKLEKTGHTAVLTLSNPPA----NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFF 62 +KLE G AV TL+ P +T TR LA++ ++E+ L++TG G K F Sbjct: 5 VKLELDGEIAVATLNRPEKLNALDTKTRMELAEVIEGIEEVA-----RVLIITGSG-KAF 58 Query: 63 SAGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIR 122 +AGAD+ D A E + + F + IAA+NGY +GGG E A+ACDIR Sbjct: 59 AAGADINELLQRDAIKAFEATKLGTDLFSRIEELEIPVIAAVNGYTLGGGCELAMACDIR 118 Query: 123 IAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEV 182 IA E+A+ PE + ++P AGGTQ LP LVG G AK+++L GE ++A+TALRIGLVEEV Sbjct: 119 IASEKAKFGQPEINLAIIPGAGGTQRLPRLVGLGMAKKLVLTGEIIDAQTALRIGLVEEV 178 Query: 183 VPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQDQ 242 V + ERA +A + ++SP +V +K+ + + + PL L+ E LF LF ++D Sbjct: 179 VEHERLMERAKEVAAKIIEKSPLAVKVAKKALNASINMPLKEGLRYEASLFALLFSSEDA 238 Query: 243 QEGVQAFLEKRSPQWKNQ 260 +EG++AFLEKR P+++ + Sbjct: 239 KEGMRAFLEKRKPEFRGR 256 >CAC2712 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Cac Clostridium acetobutylicum Length = 261 Score = 168 bits (426), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 99/262 (37%), Positives = 145/262 (55%), Gaps = 10/262 (3%) Query: 5 TNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFS 63 N+ LEK G AV+T++ P A N L ++ ++ E+ D ++ +++TG GEK F Sbjct: 4 NNVILEKEGKVAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFV 63 Query: 64 AGADLKLFADGDKAMAREMARRFG----EAFETLSAFRGVSIAAINGYAMGGGLECALAC 119 AGAD+ K M R+FG + F L IAA+NG+A+GGG E A++C Sbjct: 64 AGADISEM----KEMNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSC 119 Query: 120 DIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLV 179 DIRIA A+ PE +G+ P GGTQ L LVG G AK++I + + A+ ALRIGLV Sbjct: 120 DIRIASSNARFGQPEVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLV 179 Query: 180 EEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDT 239 +VV + A +A + +P +V SKQ I + T L E E F + F T Sbjct: 180 NKVVEPSELMNTAKEIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFST 239 Query: 240 QDQQEGVQAFLEKRSPQ-WKNQ 260 +DQ++ + AF+EKR + +KN+ Sbjct: 240 EDQKDAMTAFIEKRKIEGFKNR 261 >BH0207 [I] COG1024 Enoyl-CoA hydratase/carnithine racemase Bha Bacillus halodurans Length = 259 Score = 167 bits (422), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 97/248 (39%), Positives = 137/248 (55%), Gaps = 1/248 (0%) Query: 14 HTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSAGADLKLFA 72 H A++T + P N + L DL ++++E+ A+ I T++ TG G K FSAGADLK Sbjct: 12 HVAIITFNRPDQLNCFNYQMLLDLEQVIQEVKANPAIRTVIFTGAGGKSFSAGADLKERR 71 Query: 73 DGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRIAEEQAQMAL 132 D+ R + F + +IAAINGYA+GGG E ALACD R+A +A+M L Sbjct: 72 TLDENQVRRNVNKIRSVFNQIEELPQPTIAAINGYALGGGFELALACDFRLAVPEAKMGL 131 Query: 133 PEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVVPRGQARERA 192 E ++P AGGTQ LP L+G AK MIL G +V AE A R+G++ EV A Sbjct: 132 TEVTWAIIPGAGGTQRLPRLIGSQRAKEMILTGRKVAAEEAQRLGIILEVCDSKHLLTEA 191 Query: 193 LALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQDQQEGVQAFLEK 252 + LA++ P +V +K I L T L E + + + T+D+ E +QAF EK Sbjct: 192 VRLAEKMSVNGPLAVIQAKYAIHYGCQTDLHTGLAIEAKAYETIIPTEDRLEALQAFKEK 251 Query: 253 RSPQWKNQ 260 R PQ+K + Sbjct: 252 RPPQFKGK 259