BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0132.p (260 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0240 [R] COG3568 Metal-dependent hydrolase Bme Brucella mel... 141 2e-33 SMc01813 [R] COG3568 Metal-dependent hydrolase Sme Sinorhizobium... 138 1e-32 SMb20092 [R] COG3568 Metal-dependent hydrolase Sme Sinorhizobium... 131 1e-30 AGc4513 [R] COG3568 Metal-dependent hydrolase Atu Agrobacterium ... 124 2e-28 mlr2853 [R] COG3568 Metal-dependent hydrolase Mlo Mesorhizobium ... 122 6e-28 >BMEII0240 [R] COG3568 Metal-dependent hydrolase Bme Brucella melitensis Length = 285 Score = 141 bits (355), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 93/240 (38%), Positives = 133/240 (55%), Gaps = 10/240 (4%) Query: 25 DAPVPGRDPWPLTVATYNIHGAVGTDGRFAPERVAAVLDELQADVVALQEVPLGGADQ-- 82 DA + G D + +A+YN+H +G D +F P+R A V+ E+ ADV+ALQE ++ Sbjct: 28 DANITGGD---IVIASYNVHKCIGIDKQFNPQRTADVISEINADVIALQEADRRFGERSG 84 Query: 83 -VDVLALLRAHTGMFAAEGPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRGALDA 141 +D+ L R H + TM +GN +L R ++ L EPRGAL A Sbjct: 85 LLDLGLLERRHGLVPVPITATMPKGHGWHGNVLLLRESVVRNVQ-QLKLPGVEPRGALVA 143 Query: 142 DIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAFDTNDA-PVILLGDVNEWFMWGRT-LR 199 D+ LRV+A HLGL R Q + +L A D P +L+GD+NEW + R+ L Sbjct: 144 DLQFPAGPLRVIAAHLGLLKRSREQQAETILSALQEADTLPTLLIGDLNEWRIGKRSSLS 203 Query: 200 WLVGHFE-AAPAPPTFPSRWPVFALDRIWISPRRRLQHLAVHRSALARVASDHLPLLAHI 258 L+ F A A P+FPSR+PV ALDR+ +P + + VH + LAR+ASDHLP+ AH+ Sbjct: 204 ALMPTFNHVATAVPSFPSRFPVLALDRVLGAPDNLITSVEVHNTPLARLASDHLPIKAHL 263 >SMc01813 [R] COG3568 Metal-dependent hydrolase Sme Sinorhizobium meliloti Length = 285 Score = 138 bits (348), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 99/244 (40%), Positives = 139/244 (56%), Gaps = 10/244 (4%) Query: 21 VLSADAPVPGRDPWPLTVATYNIHGAVGTDGRFAPERVAAVLDELQADVVALQEVPLGGA 80 V++ DA V D + +A+YNIH VGTDGRF P R+A V+ E+ AD+VALQE Sbjct: 23 VVNRDAAVNRGD---IVIASYNIHKCVGTDGRFDPPRIAQVIAEIGADIVALQEADQRFG 79 Query: 81 DQVDVLAL--LRAHTGMFAAE-GPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRG 137 ++ +L L LR + + P E +GN +L R + ++ L EPRG Sbjct: 80 ERAGLLDLEHLRREAHLVSVPISPFSEKGHGWHGNVLLIREGAV-SDVRQLKLPGVEPRG 138 Query: 138 ALDADIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAF-DTNDAPVILLGDVNEWFMWGR 196 AL D+D + LRV+A HLGL R Q + LL+A D P +L+GD+NEW M R Sbjct: 139 ALVVDLDLKAGPLRVIAAHLGLLRHSRARQAETLLKATADGAGRPTLLIGDLNEWRMGKR 198 Query: 197 -TLRWLVGHFE-AAPAPPTFPSRWPVFALDRIWISPRRRLQHLAVHRSALARVASDHLPL 254 +L +L F+ + A +FPSR+P+ LDR+ +P + +AVH + LARVASDHLP+ Sbjct: 199 SSLSFLSPIFDPSHSAVASFPSRFPLLPLDRVLGNPHHLVTSVAVHDTPLARVASDHLPV 258 Query: 255 LAHI 258 A I Sbjct: 259 KASI 262 >SMb20092 [R] COG3568 Metal-dependent hydrolase Sme Sinorhizobium meliloti Length = 252 Score = 131 bits (330), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 93/237 (39%), Positives = 124/237 (52%), Gaps = 25/237 (10%) Query: 40 TYNIHGAVGTDGRFAPERVAAVLDELQADVVALQEVPL-----GGADQVDVLALLRAHTG 94 TYN+H GTD R P R+AAV+ E Q DV+ALQEV + GG DQ ++A H Sbjct: 16 TYNVHSCFGTDRRLDPARIAAVIAECQPDVIALQEVDVGRARTGGIDQAHMIA---THLN 72 Query: 95 MFAAEGPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRGALDADIDCRGQLLRVVA 154 M A P + +YG+AVL+ P+ ++ L S EPRGAL +ID L+V+ Sbjct: 73 MEAEFHPALHLEDEKYGDAVLTALPMRLIKAAPLP-SSSEPRGALWVEIDVAAVKLQVIV 131 Query: 155 THLGLKPAERRAQIKRL-----LQAFDTNDAPVILLGDVNEWFMWGRTLRWLVGHFEAAP 209 THLGL+ AER Q L L DA V+L GD+N GR+ + + + + Sbjct: 132 THLGLRGAERLRQATALLGPGWLGGMAQGDAHVVLAGDLNA---TGRSTAYRLLARQLSD 188 Query: 210 AP--------PTFPSRWPVFALDRIWISPRRRLQHLAVHRSALARVASDHLPLLAHI 258 A PTFPSR P+ +D + + + VH S LAR ASDHLPLLA + Sbjct: 189 AQLLTGVKPRPTFPSRLPLLRIDHVLVGKGIEVASCRVHGSTLARSASDHLPLLAEL 245 >AGc4513 [R] COG3568 Metal-dependent hydrolase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 319 Score = 124 bits (312), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 89/239 (37%), Positives = 126/239 (52%), Gaps = 8/239 (3%) Query: 26 APVPGRDPWPLTVATYNIHGAVGTDGRFAPERVAAVLDELQADVVALQEVPLGGADQVDV 85 AP +P+ +A+YN+H VG D +F P+R + V+ E+ AD++ALQE ++ + Sbjct: 76 APATKDHSFPV-IASYNVHKCVGRDRKFDPDRTSRVIQEIGADIIALQEADSRFGERTGL 134 Query: 86 LAL--LRAHTGMFAAEGPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRGALDADI 143 L L L +G+ +GN VL R + + L EPRGAL ++ Sbjct: 135 LDLARLERESGLVPVPVQAGVKAHGWHGNVVLFREGAV-RDVHTLKLPGLEPRGALVVEL 193 Query: 144 DCR-GQLLRVVATHLGLKPAERRAQIKRLLQAFDT-NDAPVILLGDVNEWFMW-GRTLRW 200 D + G LRV+A H GL R Q K L+ + N+ IL+GD+NEW + G +L Sbjct: 194 DLKSGGTLRVIAAHFGLLRHSRAQQAKALVGLLEARNECATILMGDLNEWRLGNGSSLNT 253 Query: 201 LVGHFEAAP-APPTFPSRWPVFALDRIWISPRRRLQHLAVHRSALARVASDHLPLLAHI 258 F P A P+FPS PV ALDRI + ++ + H SALAR+ASDHLPL A I Sbjct: 254 FRDAFGTLPPAVPSFPSNLPVLALDRIIANREGLIEQVEAHDSALARIASDHLPLKAAI 312 >mlr2853 [R] COG3568 Metal-dependent hydrolase Mlo Mesorhizobium loti Length = 246 Score = 122 bits (307), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 84/227 (37%), Positives = 121/227 (53%), Gaps = 7/227 (3%) Query: 38 VATYNIHGAVGTDGRFAPERVAAVLDELQADVVALQEVP--LGGADQVDVLALLRAHTGM 95 VA+YN+H +G D +F PER + V+ E+ DV+ALQE G D + L L TG+ Sbjct: 19 VASYNVHKCIGVDRKFDPERTSRVIREIDPDVIALQEADNRFGDRDGLLDLPRLERETGL 78 Query: 96 FAAEGPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRGALDADIDCRG-QLLRVVA 154 +GN +L + ++ + + EPRGA+ A+I+ G + LR++A Sbjct: 79 VPVPISATGKGHGWHGNVLLFKKGVV-RDVHQIRLPGLEPRGAVVAEIELNGSETLRIIA 137 Query: 155 THLGLKPAERRAQIKRLLQAFDTND-APVILLGDVNEWFMWGRT-LRWLVGHF-EAAPAP 211 HLGL R Q + +L + D P +LLGD+NEW + R+ L L F PA Sbjct: 138 AHLGLLRHSRSQQARVVLDLMSSPDEKPTLLLGDLNEWRLGNRSALNTLHATFGPQPPAV 197 Query: 212 PTFPSRWPVFALDRIWISPRRRLQHLAVHRSALARVASDHLPLLAHI 258 PTFPS P+ ALDRI + + + H + L+RVASDHLPL A + Sbjct: 198 PTFPSNLPLLALDRIMTNRHGMIAAVEAHDTPLSRVASDHLPLTAFV 244