BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0033.p (471 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGpT48 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 323 4e-88 MA4523 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A su... 207 4e-53 CAC2670 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 198 2e-50 NMA1568 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 197 4e-50 CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A s... 196 8e-50 >AGpT48 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 467 Score = 323 bits (829), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 189/454 (41%), Positives = 264/454 (58%), Gaps = 5/454 (1%) Query: 7 TLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQGGHG-P 65 +L + + L + +SCLE V+ LIA +A LN L D + LR A+ +D G G Sbjct: 6 SLAQTLERLRRKDYSCLELVETLIARCQAAKPLNALLATDWDGLRRSAKKIDRHGNAGLG 65 Query: 66 LSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAGKANMHELAFG 125 L GIP+ K NI ++A T AL N++P+ + V E LF AGAL NMHEL+FG Sbjct: 66 LCGIPLCFKANIATGIFPTSAATPALINHLPKIPSRVAERLFSAGALPGASGNMHELSFG 125 Query: 126 ITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVG 185 IT+NN G RNP++ LI GGSSGG A +A+RL +GTDTG SVR+PAALCGVVG Sbjct: 126 ITSNNYATGAVRNPWNPSLIPGGSSGGVAAAVASRLMLGGIGTDTGASVRLPAALCGVVG 185 Query: 186 LRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLLALPPSQLRLG 245 RPT RY + ++P+S TRDT G +A+ VADV +LDQ+++G SA + +P LR+G Sbjct: 186 FRPTLARYPRDRIIPVSPTRDTAGIIAQCVADVIILDQVISGR--SAKISPMPLKGLRIG 243 Query: 246 VLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDLPTLAPLSHAAGFAIVLHEFLIDL 305 + + L+ +V A + L GV ++ D+P L L+ A I L+EF L Sbjct: 244 LPTTYFYDDLDADVAFAAETTIRLLANRGVTFVEADIPHLEELNSGASLPIALYEFPHAL 303 Query: 306 PAYLQASDARDVIKRVLAEVGSPDVARIIQATLD-QPVPEHAYRLALEQRR-TMQALYRD 363 YL V+ + SPDVA I+ A +D + Y LA + R +QA YR+ Sbjct: 304 KKYLDDFVGTVSFSDVIKGIRSPDVANIVSAQIDGHQISNDEYELARQSFRPRLQATYRN 363 Query: 364 CFRSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFARNADGASIAGLPSISIPV 423 FR +DA++FPT L A+ +G++ +V G + TF + RN D +S AGLP +S+P Sbjct: 364 YFRLYQLDAILFPTAPLAAKAIGQESSVIHNGSMMNTFKIYVRNVDPSSNAGLPGLSLPA 423 Query: 424 GLSEYGLPVGLELDGPEGSDRRLLQVAASLETVL 457 L+ LPVG+E+DG GSD RLL + A+LE + Sbjct: 424 CLTPDRLPVGMEIDGLAGSDHRLLAIGAALEKAI 457 >MA4523 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Mac Methanosarcina acetivorans str.C2A Length = 476 Score = 207 bits (527), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 153/473 (32%), Positives = 243/473 (51%), Gaps = 40/473 (8%) Query: 6 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQGGHGP 65 M++ +V+ + + S E V A E + +N Y+ + L QA+ +D +G GP Sbjct: 6 MSVAQVKDKIKE---SSAEEVTAGYLEVIEKSKINGYITVSDKALE-QAKKIDVEGHEGP 61 Query: 66 LSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAGKANMHELAFG 125 L+G+PIAIKDNI V G+ ++ G+ L VP +A V+E L AGA+ GK N+ E A G Sbjct: 62 LAGVPIAIKDNISVVGLPNSCGSKILEGYVPPFNAHVIEKLLDAGAVILGKTNLDEFAMG 121 Query: 126 ITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVG 185 + +GP NP+D E + GGSSGG A++AA AP A+G+DTGGSVR PAA CGVVG Sbjct: 122 SSTETSYYGPTANPWDLERVPGGSSGGSAAVVAAGEAPFALGSDTGGSVRCPAAFCGVVG 181 Query: 186 LRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGS--RDSAPLLALPPSQ-- 241 L+PT S++GVV +++ + GP+A +V D+A+L ++AG RDS + + Q Sbjct: 182 LKPTYGAVSRYGVVAYANSLEQVGPLANNVEDIAILMDVIAGYDRRDSTSIDSKTEYQKA 241 Query: 242 -------LRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDLPTLAPLSHAAGF 294 L++GV + +G+ VE A+ + G +V +P + + A+ + Sbjct: 242 LVDDVKGLKIGVPKEFFGEGIHPGVEKAVWNAIHKFESLGATRQEVSMPNIN-YALASYY 300 Query: 295 AIVLHEFLIDLPAY--------LQASDARDVIKRVLAEVGSPDVARII---QATLDQPVP 343 I + E +L + + ++ + AE +V R I L Sbjct: 301 IIAMSEASSNLARFDGTRYGFRANGENWHAMVSKTRAEGFGTEVKRRILLGTYALSAGYH 360 Query: 344 EHAYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGE--DDTVALRGERVPTF 401 + Y AL+ R ++ + + VD L+ PT A +GE +D + L V T Sbjct: 361 DKYYLKALKVRTLVKQDFDKALST--VDLLMAPTMPNPAFRIGEKIEDPLTLYLSDVNTC 418 Query: 402 PAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLE 454 P ++AG+PS+S+P G ++ GLPVGL++ G + +L+ A + E Sbjct: 419 P--------INLAGVPSVSVPCGFTD-GLPVGLQIMGKPFDEPTVLRAAYTFE 462 >CAC2670 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Cac Clostridium acetobutylicum Length = 485 Score = 198 bits (504), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 154/485 (31%), Positives = 234/485 (48%), Gaps = 43/485 (8%) Query: 1 MDLVDMTLIEVRQGLSDRRFSCLEYVDALIAETE-AQWDLNCYLQRDPEQLRSQARMLDE 59 M+L + E++ +S + E ++ + E +N L E+ + A+ LD+ Sbjct: 1 MELYKLKAHELKDMISKKEVKVEEVTNSFLNRIEEVDEKVNALLYVAKEEAVNTAKELDK 60 Query: 60 QGGHGP----LSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAG 115 + G LSG+P+AIKDNI V + +T + L V ATV+E L + G Sbjct: 61 KIESGESLSGLSGVPVAIKDNISVKNMQNTCASKILEGYVSPYDATVIENLKKNNGVIIG 120 Query: 116 KANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVR 175 KANM E A G + N F +NP+ E + GGSSGG +A+ AP ++GT+TGGSVR Sbjct: 121 KANMDEFAMGSSTENSAFKVSKNPWSLERVPGGSSGGSAVAVASLEAPISLGTETGGSVR 180 Query: 176 IPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAG-------- 227 PA+ CG+VGL+PT R S++GVV T D G AR V D ALL Q +AG Sbjct: 181 QPASFCGLVGLKPTYGRISRYGVVAFGSTLDQVGMFARDVEDCALLTQNIAGLDKMDFTT 240 Query: 228 ----SRDSAPLLALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDLP 283 +D + L ++G+ + +GL+E V A+ E G E+ + LP Sbjct: 241 VDTPVQDYSKSLNKDLKGRKIGIPKEFFEEGLDEGVREAVKEAIKVFEENGAEVKECSLP 300 Query: 284 TLAPLSHAAGFAIVLHEFLIDLPAY---------LQASDARDV-IKRVLAEVGSPDVARI 333 L+ + AA + I E +L + +A +A D+ +K G RI Sbjct: 301 -LSDYALAAYYIISSAEASSNLARFDGVRYGYRDAEAENALDLYVKSRSKGFGEEAKRRI 359 Query: 334 IQAT--LDQPVPEHAYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGE--DD 389 + T L + + Y+ AL+ R ++ ++ F+ DA+I PTT A +GE D Sbjct: 360 MLGTYVLSKGYYDAYYKKALKVRSLIKNDFQRAFKE--FDAIITPTTPTPAFRIGEKTKD 417 Query: 390 TVALRGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQV 449 +++ + T P +IAG+PSIS+P G GLPVGL++ G + L + Sbjct: 418 VLSMYMSDIYTVP--------VNIAGIPSISVPCGFVS-GLPVGLQIMGNYFKEDTLFNL 468 Query: 450 AASLE 454 A S E Sbjct: 469 AYSYE 473 >NMA1568 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases NmA Neisseria meningitidis Z2491 Length = 481 Score = 197 bits (501), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 153/481 (31%), Positives = 235/481 (48%), Gaps = 40/481 (8%) Query: 3 LVDMTLIEVRQGLSDRRFSCLEYVDALIAE-TEAQWDLNCYLQRDPEQLRSQARMLDE-- 59 + TL + L ++ S +E A +A E LN Y+ D ++ ++AR DE Sbjct: 1 MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITIDQDKTLAEARAADERI 60 Query: 60 -QGGHGPLSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAGKAN 118 QG L+GIP+A KD G S + L N + ATVV+ L G + G+ N Sbjct: 61 AQGNASALTGIPVAYKDIFCQTGWRSACASKMLDNFISPYTATVVQKLLDEGMVTLGRTN 120 Query: 119 MHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPA 178 M E A G TN N +G +NP++ E + GGSSGG A++AARLAPAA+G+DTGGS+R PA Sbjct: 121 MDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVAARLAPAALGSDTGGSIRQPA 180 Query: 179 ALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGS--RDSAPL-- 234 + CG+ G++PT S+ G+V + + D GPMA++ D A+L MAG +DS L Sbjct: 181 SHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAEDCAILLNAMAGFDPKDSTSLER 240 Query: 235 --------LALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDLPTLA 286 L P L++G+ + +G +V + L+ G ELI+V LP Sbjct: 241 EKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTALQNTIDLLKAQGAELIEVSLPQ-T 299 Query: 287 PLSHAAGFAIVLHEFLIDLPAY---------LQASDARDVIKRVLAEVGSPDVARIIQAT 337 LS A + + E +L Y Q SD ++ + AE +V R I Sbjct: 300 KLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFSDLEEMYGKTRAEGFGSEVKRRIM-- 357 Query: 338 LDQPVPEHAYRLALEQR-RTMQALYRDCFRSQG--VDALIFPTTALTARPLGEDDT-VAL 393 + V H Y A + + ++ L D F++ D ++ PT A +G D + V Sbjct: 358 IGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFARCDLILAPTAPSAAPKIGADASPVET 417 Query: 394 RGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASL 453 + T ++AGLP++++P G S GLP+G++L G S+ ++L A + Sbjct: 418 YLSDIYTI--------AVNLAGLPALTLPAGFSSGGLPIGVQLIGNYFSEAKILGAAHQI 469 Query: 454 E 454 + Sbjct: 470 Q 470 >CAC2977 [J] COG0154 Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases Cac Clostridium acetobutylicum Length = 478 Score = 196 bits (499), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 133/484 (27%), Positives = 244/484 (50%), Gaps = 39/484 (8%) Query: 1 MDLVDMTLIEVRQGLSDRRFSCLEYVDALIAETEAQW-DLNCYLQRDPEQLRSQARMLDE 59 M+++DMT+ ++R + D+ + V A + ++N Y+ + +A+ +D+ Sbjct: 1 MEILDMTVEQLRNAILDKHLKSEDIVKAYFDNIKRNEPEINAYITLCEDYALKEAKDVDK 60 Query: 60 QGGHGP----LSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAG 115 + +G L+GIPIAIKDNI GI +T + L + VP ATV++ L A+ G Sbjct: 61 KIANGDKVGRLAGIPIAIKDNICTDGIKTTCASKMLYDFVPPYDATVIKKLKAEDAIIIG 120 Query: 116 KANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVR 175 K NM E A G + N F +NP D + GGSSGG A++AA++AP ++G+DTGGS+R Sbjct: 121 KVNMDEFAMGSSTENSAFKITKNPRDITRVPGGSSGGSAAVVAAKMAPISLGSDTGGSIR 180 Query: 176 IPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLL 235 PAA CGVVGL+PT S+ G++ + + D GP+ ++V D A L ++++G + Sbjct: 181 QPAAFCGVVGLKPTYGLVSRFGLIAFASSLDQIGPLGKTVKDCAELLEVISGEDELDNTS 240 Query: 236 ALPPSQ-------------LRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDL 282 + + +++G+ + GL+ E+ + + +L+ G E+ ++ L Sbjct: 241 SKKHEKEDYLEGIDDGIKGMKIGMPKEFLNDGLDPEIRKCIDDTIEKLKSLGAEVCEMSL 300 Query: 283 PTLAPLSHAAGFAIVLHEFLIDLPAY---------LQASDARDVIKRVLAEVGSPDVARI 333 P + +A + I E +L + D D+++ +E +V R Sbjct: 301 P-ITEEGLSAYYIISSAEASSNLARFDGIRYGYRPDDFEDVYDLMETSRSEAFGDEVKRR 359 Query: 334 IQ---ATLDQPVPEHAYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGEDDT 390 I L + Y+ AL+ ++ ++ +++ F + D ++ P + + +GE Sbjct: 360 IMLGTYALSSGYYDAYYKRALKLKKKIKNEFKEAF--ENYDLILSPVSPVLPFKIGEKKA 417 Query: 391 VALRGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVA 450 L+ + ++AG+P+IS+P +S+ GLP+GL+L GP ++++ + A Sbjct: 418 DPLQMYLADIYTV------NINLAGIPAISLPCSVSKEGLPIGLQLLGPHFGEKKIFRAA 471 Query: 451 ASLE 454 +LE Sbjct: 472 RALE 475