BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0087.p (456 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2211 [LKJ] COG0513 Superfamily II DNA and RNA helicases Rso R... 505 e-143 STM0820 [LKJ] COG0513 Superfamily II DNA and RNA helicases Sty S... 382 e-106 alr4718 [LKJ] COG0513 Superfamily II DNA and RNA helicases Nos N... 380 e-105 rhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases Eco Esch... 379 e-105 ZrhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases EcZ Esc... 379 e-105 >RSc2211 [LKJ] COG0513 Superfamily II DNA and RNA helicases Rso Ralstonia solanacearum Length = 495 Score = 505 bits (1301), Expect = e-143, Method: Compositional matrix adjust. Identities = 281/498 (56%), Positives = 330/498 (66%), Gaps = 49/498 (9%) Query: 3 DTTSASAAPAAPAVRFEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQT 62 +T + SAAP++ V F+ FGL PDIL+ALAE GY TPIQA AIPVV+ GRDVMGAAQT Sbjct: 2 NTETTSAAPSSDPVTFDTFGLHPDILRALAESGYTRATPIQAAAIPVVIAGRDVMGAAQT 61 Query: 63 GTGKTAGFSLPIIQRLLQHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLR 122 GTGKTAGFSLPIIQ LL A+ SASPARHPVRALILTPTRELADQV DNVA Y + T LR Sbjct: 62 GTGKTAGFSLPIIQNLLPDANTSASPARHPVRALILTPTRELADQVYDNVAKYAKHTALR 121 Query: 123 STVVFGGVDMAPQTAILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRML--- 179 S VVFGGVDM PQT LR GVE+++ATPGRLLDHVQQ++VNLSQ ++LV+DEADRML Sbjct: 122 SAVVFGGVDMNPQTEQLRRGVEVLVATPGRLLDHVQQRSVNLSQVRMLVLDEADRMLDMG 181 Query: 180 ----------------------------XPKLAASFQNNPVTIEVARSNATAENVTQTIY 211 KLAAS+ +P TIEVARSNATA+NV Q IY Sbjct: 182 FLPDLQRIINLLPAHRQTLLFSATFSPEIKKLAASYLRHPQTIEVARSNATADNVRQVIY 241 Query: 212 KVEEAAKADAVSFIIRQRELK----QVIVFSNTKIGASRLARTLVAEGVKASAIHGDKTQ 267 V + K A+ ++RQR + Q IVFSN+KIG SRLAR L E + A+AIHGDKTQ Sbjct: 242 TVPDGHKQAALVHLLRQRAEQGLPSQCIVFSNSKIGCSRLARALEREKINANAIHGDKTQ 301 Query: 268 SERMAALEAFKQGQIEVLVATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGA 327 +ERM LEAFKQG ++VLVATDVAARGLDI+++PCVIN+DLP+NAEDYVHRIGRTGRAGA Sbjct: 302 TERMQTLEAFKQGTVDVLVATDVAARGLDISQMPCVINFDLPFNAEDYVHRIGRTGRAGA 361 Query: 328 SGDAISLFCDKDDRLLTDIEKLIKKKFERAELTGFAPRARHERSDRAERGERSERGERSD 387 SGDA+SLF D+RLL DIEKLIK+ R LT F P R + Sbjct: 362 SGDALSLFAPGDERLLADIEKLIKRNLPRETLTDFDPTGEQARERERRERDERRNRTEIR 421 Query: 388 RGDRSDRGERSRSSREGGAAPMRREKVDPWFLKPYEPSAVETVST---------KPELAK 438 R D +R P R DP+F +PYE + + P AK Sbjct: 422 RARERDERNGARVLDHTVVRPPFRPSEDPFFSRPYESNVPAEAAEAAKPAPAGGHPRSAK 481 Query: 439 NNRPKAKVAALLGGMPKR 456 RP +AALLGG+P++ Sbjct: 482 --RP---IAALLGGVPRK 494 >STM0820 [LKJ] COG0513 Superfamily II DNA and RNA helicases Sty Salmonella typhimurium LT2 Length = 454 Score = 382 bits (981), Expect = e-106, Method: Compositional matrix adjust. Identities = 199/378 (52%), Positives = 254/378 (67%), Gaps = 33/378 (8%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F+ GL+PDIL+A+AEQGY PTPIQ QAIP VL+GRD+M +AQTGTGKTAGF+LP++Q Sbjct: 3 FDSLGLNPDILRAIAEQGYREPTPIQQQAIPAVLEGRDLMASAQTGTGKTAGFTLPLLQH 62 Query: 78 LLQHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L+ H H+ R PVRALILTPTRELA Q+ +NV Y ++ +RS VVFGGV + PQ Sbjct: 63 LITHQPHAK--GRRPVRALILTPTRELAAQIGENVRDYSKYLNIRSLVVFGGVSINPQMM 120 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRML-----------XPKLAAS 186 LR GV++++ATPGRLLD Q V L Q +ILV+DEADRML KL A Sbjct: 121 KLRGGVDVLVATPGRLLDLEHQNAVKLDQIEILVLDEADRMLDMGFIHDIRRVLAKLPAK 180 Query: 187 FQN--------------------NPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 226 QN NP+ IEVAR N +E VTQ ++ V++ K + +S +I Sbjct: 181 RQNLLFSATFSDDIKALAEKLLRNPLEIEVARRNTASEQVTQHVHFVDKKRKRELLSQMI 240 Query: 227 RQRELKQVIVFSNTKIGASRLARTLVAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 286 Q +QV+VF+ TK GA+ LA L +G++++AIHG+K+Q R AL FK G I VLV Sbjct: 241 GQGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIHGNKSQGARTRALADFKSGDIRVLV 300 Query: 287 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDDRLLTDI 346 ATD+AARGLDI ELP V+NY+LP EDYVHRIGRTGRA A+G+A+SL C + +LL DI Sbjct: 301 ATDIAARGLDIEELPHVVNYELPNVPEDYVHRIGRTGRAAATGEALSLVCVDEHKLLRDI 360 Query: 347 EKLIKKKFERAELTGFAP 364 EKL+KK+ R G+ P Sbjct: 361 EKLLKKEIPRITTPGYEP 378 >alr4718 [LKJ] COG0513 Superfamily II DNA and RNA helicases Nos Nostoc sp. PCC 7120 Length = 426 Score = 380 bits (977), Expect = e-105, Method: Compositional matrix adjust. Identities = 208/404 (51%), Positives = 263/404 (65%), Gaps = 35/404 (8%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F GLS +I+ A+ E GY PTPIQ Q+IP VL GRD++ AQTGTGKTA F+LP++ Sbjct: 3 FSHLGLSNEIINAVTELGYTKPTPIQMQSIPAVLSGRDLLAGAQTGTGKTASFTLPLLHL 62 Query: 78 LLQHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L Q + SAS A P+RALILTPTRELA QV +V Y ++ L S V+FGGV + PQ Sbjct: 63 LSQDSLKSASNASSPIRALILTPTRELAAQVESSVRDYGKYLKLNSMVMFGGVSINPQKQ 122 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRML--------------XPK- 182 L+ V+I++ATPGRLLDHVQQ TVNLSQ +ILV+DEADRML PK Sbjct: 123 RLKGRVDILVATPGRLLDHVQQGTVNLSQIEILVLDEADRMLDMGFIRDIRRILSLLPKQ 182 Query: 183 ----------------LAASFQNNPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 226 LAA N P IEVAR N TA+ VTQ +YK+E K D ++ +I Sbjct: 183 RQNLLFFATFSDKIKELAAGLLNRPQMIEVARRNVTADTVTQKVYKIERDRKRDLLAHLI 242 Query: 227 RQRELKQVIVFSNTKIGASRLARTLVAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 286 R+ QV+VF+ TK GA RL + L E ++A AIHG+K+QS R AL FK G ++VLV Sbjct: 243 RKDNWYQVLVFTRTKYGADRLVKQLGHERIQALAIHGNKSQSARTHALAKFKNGSLQVLV 302 Query: 287 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDDRLLTDI 346 ATD+AARGLDI+ELP V+N+DLPY EDYVHRIGRTGRAGASG+A+SL + LL DI Sbjct: 303 ATDIAARGLDISELPYVVNFDLPYVPEDYVHRIGRTGRAGASGEAVSLVSADEYHLLADI 362 Query: 347 EKLIKKK--FERAELTGFAPRARHE--RSDRAERGERSERGERS 386 EKLI+K+ FE G +A+ E + +R ++ + S+ RS Sbjct: 363 EKLIEKRLPFELVAGIGANSQAKPEPTQDERKQKPKDSQHQPRS 406 >rhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases Eco Escherichia coli K12 Length = 454 Score = 379 bits (973), Expect = e-105, Method: Compositional matrix adjust. Identities = 197/378 (52%), Positives = 254/378 (67%), Gaps = 33/378 (8%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F+ GLSPDIL+A+AEQGY PTPIQ QAIP VL+GRD+M +AQTGTGKTAGF+LP++Q Sbjct: 3 FDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMASAQTGTGKTAGFTLPLLQH 62 Query: 78 LLQHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L+ H+ R PVRALILTPTRELA Q+ +NV Y ++ +RS VVFGGV + PQ Sbjct: 63 LITRQPHAK--GRRPVRALILTPTRELAAQIGENVRDYSKYLNIRSLVVFGGVSINPQMM 120 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRML-----------XPKLAAS 186 LR GV++++ATPGRLLD Q V L Q +ILV+DEADRML KL A Sbjct: 121 KLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADRMLDMGFIHDIRRVLTKLPAK 180 Query: 187 FQN--------------------NPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 226 QN NP+ IEVAR N ++ VTQ ++ V++ K + +S +I Sbjct: 181 RQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASDQVTQHVHFVDKKRKRELLSHMI 240 Query: 227 RQRELKQVIVFSNTKIGASRLARTLVAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 286 + +QV+VF+ TK GA+ LA L +G++++AIHG+K+Q R AL FK G I VLV Sbjct: 241 GKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIHGNKSQGARTRALADFKSGDIRVLV 300 Query: 287 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDDRLLTDI 346 ATD+AARGLDI ELP V+NY+LP EDYVHRIGRTGRA A+G+A+SL C + +LL DI Sbjct: 301 ATDIAARGLDIEELPHVVNYELPNVPEDYVHRIGRTGRAAATGEALSLVCVDEHKLLRDI 360 Query: 347 EKLIKKKFERAELTGFAP 364 EKL+KK+ R + G+ P Sbjct: 361 EKLLKKEIPRIAIPGYEP 378 >ZrhlE [LKJ] COG0513 Superfamily II DNA and RNA helicases EcZ Escherichia coli O157:H7 EDL933 Length = 455 Score = 379 bits (973), Expect = e-105, Method: Compositional matrix adjust. Identities = 197/378 (52%), Positives = 254/378 (67%), Gaps = 33/378 (8%) Query: 18 FEDFGLSPDILKALAEQGYVHPTPIQAQAIPVVLQGRDVMGAAQTGTGKTAGFSLPIIQR 77 F+ GLSPDIL+A+AEQGY PTPIQ QAIP VL+GRD+M +AQTGTGKTAGF+LP++Q Sbjct: 3 FDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMASAQTGTGKTAGFTLPLLQH 62 Query: 78 LLQHASHSASPARHPVRALILTPTRELADQVADNVAAYCRFTPLRSTVVFGGVDMAPQTA 137 L+ H+ R PVRALILTPTRELA Q+ +NV Y ++ +RS VVFGGV + PQ Sbjct: 63 LITRQPHAK--GRRPVRALILTPTRELAAQIGENVRDYSKYLNIRSLVVFGGVSINPQMM 120 Query: 138 ILRAGVEIVIATPGRLLDHVQQKTVNLSQTQILVMDEADRML-----------XPKLAAS 186 LR GV++++ATPGRLLD Q V L Q +ILV+DEADRML KL A Sbjct: 121 KLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADRMLDMGFIHDIRRVLTKLPAK 180 Query: 187 FQN--------------------NPVTIEVARSNATAENVTQTIYKVEEAAKADAVSFII 226 QN NP+ IEVAR N ++ VTQ ++ V++ K + +S +I Sbjct: 181 RQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASDQVTQHVHFVDKKRKRELLSHMI 240 Query: 227 RQRELKQVIVFSNTKIGASRLARTLVAEGVKASAIHGDKTQSERMAALEAFKQGQIEVLV 286 + +QV+VF+ TK GA+ LA L +G++++AIHG+K+Q R AL FK G I VLV Sbjct: 241 GKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIHGNKSQGARTRALADFKSGDIRVLV 300 Query: 287 ATDVAARGLDIAELPCVINYDLPYNAEDYVHRIGRTGRAGASGDAISLFCDKDDRLLTDI 346 ATD+AARGLDI ELP V+NY+LP EDYVHRIGRTGRA A+G+A+SL C + +LL DI Sbjct: 301 ATDIAARGLDIEELPHVVNYELPNVPEDYVHRIGRTGRAAATGEALSLVCVDEHKLLRDI 360 Query: 347 EKLIKKKFERAELTGFAP 364 EKL+KK+ R + G+ P Sbjct: 361 EKLLKKEIPRIAIPGYEP 378