BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0031.p (199 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2306 [E] COG1280 Putative threonine efflux protein Pae Pseudom... 157 1e-38 SMc01425 [E] COG1280 Putative threonine efflux protein Sme Sinor... 154 9e-38 mll1430 [E] COG1280 Putative threonine efflux protein Mlo Mesorh... 145 4e-35 VC1712 [E] COG1280 Putative threonine efflux protein Vch Vibrio ... 137 1e-32 YPO0181 [E] COG1280 Putative threonine efflux protein Ype Yersin... 110 2e-24 >PA2306 [E] COG1280 Putative threonine efflux protein Pae Pseudomonas aeruginosa Length = 205 Score = 157 bits (398), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 86/193 (44%), Positives = 119/193 (61%), Gaps = 4/193 (2%) Query: 2 IESLLPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHMLGITFGFGVLLALCAFGIG 61 ++ LL FAFV+S+TPGPNN ML +SG+ FGF RS+PH+LGI+ GF +++ FG+G Sbjct: 5 VDLLLAFSLFAFVTSVTPGPNNTMLLASGVNFGFVRSIPHILGISCGFFIMVMAVGFGLG 64 Query: 62 ELVIAVPQLVLLLKAMGCAYLLYLAWQLRRMGVAGAG-ERAAQPMSFGAAALFQFANPKA 120 + A P L +L+ +G AYLLYLAW++ G AG E +P+S+ AA FQ+ NPKA Sbjct: 65 TVFEAYPVLYTILRYVGAAYLLYLAWKIATSGPAGNDPEGRGKPLSYWGAAAFQWVNPKA 124 Query: 121 WVMAVTGASAFMPMLHKLPGALAILVYCLVFCAINLPCVSVWAGAGAVLRRYLERPLWRM 180 WVMAV S + P+ ++V VF IN P + +WAG G++LR L P W Sbjct: 125 WVMAVGAISTYTPLQGYFTN---VVVISTVFALINAPTIGIWAGFGSMLRNVLRDPRWLR 181 Query: 181 AFAAVMVLLTLSS 193 F M LL ++S Sbjct: 182 VFNVGMALLLVAS 194 >SMc01425 [E] COG1280 Putative threonine efflux protein Sme Sinorhizobium meliloti Length = 197 Score = 154 bits (390), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 84/175 (48%), Positives = 114/175 (65%), Gaps = 3/175 (1%) Query: 2 IESLLPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHMLGITFGFGVLLALCAFGIG 61 +++LL L FAF +SITPGPNN+ML +SG+ FGF R++PHMLGI GF VLL G+G Sbjct: 3 LDTLLALFLFAFTTSITPGPNNMMLFASGVNFGFVRTIPHMLGIGIGFFVLLIAVGLGLG 62 Query: 62 ELVIAVPQLVLLLKAMGCAYLLYLAWQLRRMGVAGAGERAAQPMSFGAAALFQFANPKAW 121 L+ +VP L LK G AYL+++AW++ G G A PM+F AA FQ+ NPKAW Sbjct: 63 ALLHSVPLLYTALKFAGGAYLVWIAWKIGTSRSLGEGRANAVPMTFLQAAAFQWVNPKAW 122 Query: 122 VMAVTGASAFMPMLHKLPGALAILVYCLVFCAINLPCVSVWAGAGAVLRRYLERP 176 VMAV+ + + L +++LV LVF +N+P VS WAG G+ LR++L P Sbjct: 123 VMAVSAMATYTSSDSYL---MSVLVVGLVFALVNVPSVSTWAGFGSALRQWLSEP 174 >mll1430 [E] COG1280 Putative threonine efflux protein Mlo Mesorhizobium loti Length = 198 Score = 145 bits (367), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 84/177 (47%), Positives = 113/177 (63%), Gaps = 4/177 (2%) Query: 2 IESLLPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHMLGITFGFGVLLALCAFGIG 61 +++ L L+ +AFV+SITPGPNN+ML +SG+ FG R++PHMLGI+ GF VLL G+G Sbjct: 3 LDAFLALLVYAFVTSITPGPNNLMLLASGVNFGIARTVPHMLGISMGFLVLLLAVGLGLG 62 Query: 62 ELVIAVPQLVLLLKAMGCAYLLYLAWQLR-RMGVAGAGERAAQPMSFGAAALFQFANPKA 120 ++ A P L LK G AYLLYLAW++ + G GE A+PM F AA FQ+ NPKA Sbjct: 63 AVLTAFPALHTALKIAGGAYLLYLAWKIAMSRSLTGKGETKARPMRFIDAAAFQWVNPKA 122 Query: 121 WVMAVTGASAFMPMLHKLPGALAILVYCLVFCAINLPCVSVWAGAGAVLRRYLERPL 177 WVMA+T + + H L++ + F +NLP VSVWAG G LR +L P+ Sbjct: 123 WVMAITAMAVYANAEHPF---LSVALISTAFTIVNLPSVSVWAGFGTALRGFLSDPV 176 >VC1712 [E] COG1280 Putative threonine efflux protein Vch Vibrio cholerae Length = 204 Score = 137 bits (345), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 82/180 (45%), Positives = 106/180 (58%), Gaps = 5/180 (2%) Query: 7 PLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHMLGITFGFGVLLALCAFGIGELVIA 66 PL++FAFVS+ +PGPNNIML +SG GF R++PHMLGITFGF +++ L G+ EL Sbjct: 18 PLLAFAFVSTFSPGPNNIMLMTSGANVGFLRTIPHMLGITFGFSIMVLLVGVGLTELFQR 77 Query: 67 VPQLVLLLKAMGCAYLLYLAWQLRRMGVAGAGERAAQPMSFGAAALFQFANPKAWVMAVT 126 P L L+ + YL+YLA ++ + G+ QPMSF AAALFQ+ NPK W MA+T Sbjct: 78 YPILQQGLQILCTLYLVYLAVKIALSRPSKEGQE-YQPMSFVAAALFQWVNPKGWSMALT 136 Query: 127 GASAFMPMLHKLPGALAILVYCLVFCAINLPCVSVWAGAGAVLRRYLERPLWRMAFAAVM 186 S F P L L LV+ LV NLP VS W AG L ++ P + F M Sbjct: 137 AVSVFNPNASWLQLGLIALVFALV----NLPSVSAWTAAGKQLSYWMNHPNYVRWFNGAM 192 >YPO0181 [E] COG1280 Putative threonine efflux protein Ype Yersinia pestis Length = 200 Score = 110 bits (274), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 77/199 (38%), Positives = 108/199 (54%), Gaps = 12/199 (6%) Query: 6 LPLMSFAFVSSITPGPNNIMLTSSGIWFGFRRSLPHMLGITFGFGVLLALCAFGIGELVI 65 L ++ F +V++ITPGPNN++LTS+G FGF RS+ MLGI G +L L AFG+G L++ Sbjct: 7 LSMVGFLWVAAITPGPNNMLLTSTGANFGFMRSIWLMLGIMLGMQSMLLLVAFGVGSLIL 66 Query: 66 AVPQLVLLLKAMGCAYLLYLAWQLRRMGVAGAGERA--AQPMSFGAAALFQFANPKAWVM 123 P L L LK +G YLL+LAW++ A+P+ L QF NPKAW+M Sbjct: 67 IYPSLHLALKILGSLYLLWLAWKIATSAYEKLDTNVAPAKPLRLYQGWLLQFLNPKAWLM 126 Query: 124 AVTGASAFMPMLHKLPGA---LAILVYCLVFCAINLPCVSVWAGAGAVLRRYL-ERPLWR 179 + ++F + GA +IL+ L +NL +W G G ++ R L R W Sbjct: 127 GLGSVASF-----SMAGAAYNHSILMISLGMFMVNLVAGIIWLGFGTLIGRLLGSRRAW- 180 Query: 180 MAFAAVMVLLTLSSALAIW 198 F M LLT + L IW Sbjct: 181 FTFNISMGLLTAACVLLIW 199