BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0012.p (358 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0781 [R] COG2070 Dioxygenases related to 2-nitropropane dioxyg... 282 1e-75 BS_yrpB [R] COG2070 Dioxygenases related to 2-nitropropane dioxy... 237 3e-62 BH1205 [R] COG2070 Dioxygenases related to 2-nitropropane dioxyg... 224 2e-58 RSp0307 [R] COG2070 Dioxygenases related to 2-nitropropane dioxy... 208 2e-53 PA4202 [R] COG2070 Dioxygenases related to 2-nitropropane dioxyg... 205 1e-52 >SA0781 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Sau Staphylococcus aureus N315 Length = 355 Score = 282 bits (721), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 144/345 (41%), Positives = 218/345 (63%), Gaps = 5/345 (1%) Query: 9 LTRKFGIDFPIVQGPMNGGSTPEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIRALTAK 68 LT+ I++PI+Q M G +TP++VA+VSN+GG+G + A F+ Q + +++ +R LT+ Sbjct: 7 LTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIGAGYFNTQQLEDEIDYVRQLTSN 66 Query: 69 PFCVNLFVLETPAPTPGEIRDALDRLQPIRAELGLPPAAAPERYCEEFREQLDALVALKA 128 F VN+FV + T +I + L+P R L L ++F+ +D ++ + Sbjct: 67 SFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLEEPVVKIIEEQQFKCHIDTIIKKQV 126 Query: 129 PVASFTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAICAQGAEAGGHRGTF 188 PV FTFG+ + + IERL A +IGTAT+V EA+A + G DAI AQG+EAGGHRG+F Sbjct: 127 PVCCFTFGIPNESIIERLKEANIKLIGTATSVDEAIANEKAGMDAIVAQGSEAGGHRGSF 186 Query: 189 IGHPDA-SMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQAAQLGTAFLMTA 247 + + M+GT++LVPQIVD V++PVIAAGGIMDGRG+ A++ LGA+ Q+GTAFL + Sbjct: 187 LKPKNQLPMVGTISLVPQIVDVVSIPVIAAGGIMDGRGVLASIVLGAEGVQMGTAFLTSQ 246 Query: 248 ESPIHAAWQEKIRLSTDTSTRTTRTFTGRPARGILNDFMQRMGRYEQELPAYPVQHALTT 307 +S ++ I S +T T T+ F+G+ ARGI N F++ M +YE ++P YP+Q+ LT+ Sbjct: 247 DSNASELLRDAIINSKETDTVVTKAFSGKLARGINNRFIEEMSQYEGDIPDYPIQNELTS 306 Query: 308 EIRKAAGQANRPEFMSLWAGQAAGMSRRRAEAIPAAELVRVLVAE 352 IRKAA E +W+GQ S R A PA ++ ++ + Sbjct: 307 SIRKAAANIGDKELTHMWSGQ----SPRLATTHPANTIMSNIINQ 347 >BS_yrpB [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Bsu Bacillus subtilis Length = 347 Score = 237 bits (604), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 131/346 (37%), Positives = 199/346 (57%), Gaps = 8/346 (2%) Query: 9 LTRKFGIDFPIVQGPMNGGST-PEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIRALTA 67 +KF + PI+Q PM GG T P + +AVSN G +G+LA+ + ++ I LT Sbjct: 4 FMKKFSLTKPIIQAPMAGGITKPRLASAVSNQGALGSLASGYLTPDLLEQQIKEIFELTD 63 Query: 68 KPFCVNLFV-LETPAPTPGEIRDALDRLQPIRAELGLPPAAAPERYCEEFREQLDALVAL 126 PF +N+FV L P +I+ + + P+ ++ + E ++F +++D ++ Sbjct: 64 APFQINVFVPLGLEMPPKDQIKKWKENI-PLANQVNQFTSVQEE--WDDFYQKIDLILKY 120 Query: 127 KAPVASFTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAICAQGAEAGGHRG 186 K SFTF L +++ L AG +IGTA+ V EAL + G D + QG+EAGGHRG Sbjct: 121 KVKACSFTFDLPPEDAVKELKTAGCCLIGTASTVEEALLMEERGMDIVVLQGSEAGGHRG 180 Query: 187 TFIGHPDASMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQAAQLGTAFLMT 246 F+ S +G +AL+PQ DA+++PVIAAGG++D RG+ AALTLGAQ Q+G+AFL+ Sbjct: 181 AFLPSKGESAVGLMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLIC 240 Query: 247 AESPIHAAWQEKIRLSTDTSTRTTRTFTGRPARGILNDFMQRMGRYEQELPAYPVQHALT 306 ES H ++KI + + T+ T F+G+ ARGI+N +M+ ++E + YP Q+ LT Sbjct: 241 HESNAHPVHKQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQNTLT 300 Query: 307 TEIRKAAGQANRPEFMSLWAGQAAGMSRRRAEAIPAAELVRVLVAE 352 +R+ A N + MSLWAGQ R E I +L+ L E Sbjct: 301 KAMRQKASLQNNHDQMSLWAGQGI---RSLTEEISVKQLLNQLCQE 343 >BH1205 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Bha Bacillus halodurans Length = 365 Score = 224 bits (572), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 147/339 (43%), Positives = 200/339 (58%), Gaps = 8/339 (2%) Query: 5 LSTPLTRKFGIDFPIVQGPMNGG-STPEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIR 63 T L ++ I+FPIVQ M GG +TPE+VAAVSN GG+G + A V+ + + IR Sbjct: 2 FQTALCKRLSIEFPIVQAGMAGGPTTPELVAAVSNHGGLGTIGAGYMTVEALEVAIKRIR 61 Query: 64 ALTAKPFCVNLFVLETPAPTP--GEIRDALDRLQPIRAELGLPPAAAPERYCEEFREQLD 121 LT +PF VNLF + E++ LD+ IRA LGL + FR++ D Sbjct: 62 QLTDRPFAVNLFCTDWACDYSRLEEVKPVLDQ---IRANLGLGKLEEVPAGRDMFRDKFD 118 Query: 122 ALVALKAPVASFTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAICAQGAEA 181 L+ + PV S FGLL +ERL G L T V EA + GAD I AQG++A Sbjct: 119 LLLKERVPVFSSAFGLLAKHDVERLKEFGILWTAMVTTVGEAQEAEQQGADVIVAQGSDA 178 Query: 182 GGHRGTFI--GHPDASMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQAAQL 239 GGHRGTF D + IGT +LVPQIVD V +PV+AAGG++DGRG+ AAL LGA Q+ Sbjct: 179 GGHRGTFSVDEKKDGASIGTFSLVPQIVDHVQVPVVAAGGVVDGRGLVAALALGAAGVQM 238 Query: 240 GTAFLMTAESPIHAAWQEKIRLSTDTSTRTTRTFTGRPARGILNDFMQRMGRYEQELPAY 299 GT FL+T E+ + +++ + ST+ T TR F+GRPARGI N+F+ + E + Sbjct: 239 GTRFLLTKEAGTASYYRKALLTSTEEDTVLTRAFSGRPARGIDNEFINQFETSGVEPLPF 298 Query: 300 PVQHALTTEIRKAAGQANRPEFMSLWAGQAAGMSRRRAE 338 P Q+ LT+ IRK AG+ + E+ SLW+GQ + +R E Sbjct: 299 PAQNHLTSTIRKEAGRQQKQEYSSLWSGQGLRLLQRDGE 337 >RSp0307 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Rso Ralstonia solanacearum Length = 362 Score = 208 bits (529), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 138/338 (40%), Positives = 187/338 (55%), Gaps = 3/338 (0%) Query: 6 STPLTRKFGIDFPIVQGPMNGGSTPEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIRAL 65 T L F ID PIV P+ G E+ AV+ AGG+G+L A + +R +V RA Sbjct: 6 DTRLLALFQIDVPIVLAPLAGVGGVELAVAVARAGGLGSLPCAMLNADQIREQVGQFRAA 65 Query: 66 TAKPFCVNLFVLETPAPTPGEIRDALDRLQPIRAELGLPPAA-APERYCEEFREQLDALV 124 P +N F P P P L P AE G+ PAA AP F A+V Sbjct: 66 VRAPINLNFFCHVPPQPDPEREARWRAHLAPYYAEFGIDPAATAPFVNRAPFDAAACAVV 125 Query: 125 -ALKAPVASFTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAICAQGAEAGG 183 +L+ V SF FGL +A + R+ AAG+ ++ +AT V EA +ANG DA+ AQGAEAGG Sbjct: 126 ESLRPEVVSFHFGLPEAGLLARVRAAGARILASATTVEEACWLEANGCDAVIAQGAEAGG 185 Query: 184 HRGTFIGHPDASMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQAAQLGTAF 243 HRG F+ S +GT ALVPQ+ DAV +PVIA GG+ D RG+AAA LGA Q+GTA+ Sbjct: 186 HRGMFLATAVESQVGTFALVPQVADAVRVPVIATGGVADARGMAAAFALGAAGVQIGTAY 245 Query: 244 LMTAESPIHAAWQEKIRLSTDTSTRTTRTFTGRPARGILNDFMQRMGRYEQELPAYPVQH 303 +++ E+ A + ++ + D T T FTGRPARGI+N M+ +G PA+P Sbjct: 246 MLSPEAKTSAIHRAALKRAADGDTALTNLFTGRPARGIVNRLMREIGPMSSFAPAFPTAG 305 Query: 304 ALTTEIRKAAGQANRPEFMSLWAGQAAGM-SRRRAEAI 340 +R A +F++LW+GQA + + AEAI Sbjct: 306 GALAPLRAKTEPAGSGDFINLWSGQAPRLATEDSAEAI 343 >PA4202 [R] COG2070 Dioxygenases related to 2-nitropropane dioxygenase Pae Pseudomonas aeruginosa Length = 351 Score = 205 bits (521), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 147/350 (42%), Positives = 188/350 (53%), Gaps = 8/350 (2%) Query: 9 LTRKFGIDFPIVQGPMNGGSTPEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIRALTAK 68 TR GI PI+Q PM G STP + AAVSNAGG+G++A + R + +R LT K Sbjct: 5 FTRLLGIQQPIIQAPMLGVSTPALAAAVSNAGGLGSIAITGSAAEKGRALIREVRGLTDK 64 Query: 69 PFCVNLFVLETPAPTPGEIRDALDRLQPIRAELGLPPAAAPERYCEEFREQ---LDALVA 125 PF VNLF P R LD L+P+ AE G P + F E L L+ Sbjct: 65 PFNVNLFCHRPGQADPARERAWLDYLKPLFAEFGAEPPVRLKNIYLSFLEDPTLLPMLLE 124 Query: 126 LKAPVASFTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAICAQGAEAGGHR 185 + SF FG + L A G V+ AT EA +A GADA+ AQG EAGGHR Sbjct: 125 ERPAAVSFHFGAPPRDQVRALQAVGIRVLVCATTPEEAALVEAAGADAVVAQGIEAGGHR 184 Query: 186 GTFIGHPDASMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQAAQLGTAFLM 245 G F + IGTLALV + +LPV+AAGGIMDGRGI AAL LGA A Q+GTAF++ Sbjct: 185 GVFEPERGDAAIGTLALVRLLAARGSLPVVAAGGIMDGRGIRAALELGASAVQMGTAFVL 244 Query: 246 TAESPIHAAWQEKIRLSTDTSTRTTRTFTGRPARGILND-FMQRMGRYEQELPAYPVQHA 304 ES +AA++E ++ T T T +GR ARG+ N F LP YP + Sbjct: 245 CPESSANAAYREALKGPRAARTALTVTMSGRSARGLPNRMFFDAAAPGVPPLPDYPFVYD 304 Query: 305 LTTEIRKAAGQANRPEFMSLWAGQAAGMSRRRAEAIPAAELVRVLVAETQ 354 T ++ AA +F + WAGQ A ++R +PAAEL+R LV E + Sbjct: 305 ATKALQTAALARGNHDFAAQWAGQGAALARE----LPAAELLRTLVEELR 350