BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0002.p (303 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2564 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate trans... 368 e-102 PM0905 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transf... 321 1e-87 HI0068 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transf... 318 8e-87 VC0346 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transf... 317 1e-86 STM4360 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate trans... 316 5e-86 >RSc2564 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Rso Ralstonia solanacearum Length = 323 Score = 368 bits (945), Expect = e-102, Method: Compositional matrix adjust. Identities = 198/309 (64%), Positives = 231/309 (74%), Gaps = 7/309 (2%) Query: 1 MGPTASGKTAAALEIARHLPCEIISVDSALVYREMDIGTAKPTPAELAQVQHHLIDIIDP 60 +GPTASGKTAAAL +A P EIIS+DSALVYR+MDIGTAKP+ AE A HHLIDIIDP Sbjct: 15 LGPTASGKTAAALALAERWPVEIISMDSALVYRDMDIGTAKPSRAEQAIAPHHLIDIIDP 74 Query: 61 TEAYSAMQFRQDALRLTQEIIARGRLPLLVGGTMLYFKVLRDGLDELPQADPALRAQLDE 120 +AYSA QF DA L + I ARGRLPL+VGGTMLY+K L GL +LP ADPA+RA++D Sbjct: 75 LDAYSAAQFATDAQALIEAIRARGRLPLIVGGTMLYYKALTQGLSDLPGADPAIRAEIDA 134 Query: 121 EAARVGMPAMHARLAELDPVTAARLKPNDSQRIQRALEISLLTGQPMSALLAQKPAEPLP 180 EAAR G PA+HA+LA++DPVTAARL D+QRIQRALE+ LTGQPMSALLA++ Sbjct: 135 EAARDGWPALHAKLAQVDPVTAARLHATDAQRIQRALELYRLTGQPMSALLAREAGAAAF 194 Query: 181 MR------LLPLALEPSERAVLHDRIALRFDLMLRDGGLEHEVRALRARGDLHLGLPSMR 234 R L +ALEP++RAVLH RIA RFD ML GGL EV ALR RGDL LPS+R Sbjct: 195 HRHEAAAAYLSIALEPADRAVLHARIAQRFDAMLA-GGLLDEVEALRRRGDLSPVLPSIR 253 Query: 235 CVGYRQMWEYLDGSIDAAAMREKGIAATRQLAKRQLTWLRSMPERISIDCNRPEATAEVL 294 CVGYRQ W YLDG ID A +RE+GIAATRQL KRQ+TWLRS PER +DC P+ +V Sbjct: 254 CVGYRQAWAYLDGEIDMATLREQGIAATRQLCKRQITWLRSTPERRVVDCLAPDYVDQVA 313 Query: 295 ARVREAVDN 303 VR A++ Sbjct: 314 RLVRNALET 322 >PM0905 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Pmu Pasteurella multocida Length = 357 Score = 321 bits (823), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 160/274 (58%), Positives = 202/274 (73%), Gaps = 2/274 (0%) Query: 1 MGPTASGKTAAALEIARHLPCEIISVDSALVYREMDIGTAKPTPAELAQVQHHLIDIIDP 60 MGPTASGKT A+++ + LP E+ISVDSAL+YR MDIGTAKP+ ELA H LIDI DP Sbjct: 53 MGPTASGKTDLAIQLRQTLPVEVISVDSALIYRGMDIGTAKPSAEELALAPHRLIDICDP 112 Query: 61 TEAYSAMQFRQDALRLTQEIIARGRLPLLVGGTMLYFKVLRDGLDELPQADPALRAQLDE 120 E+YSA FRQDALR +IIA G++PLLVGGTMLY+K L +GL LP AD +R++++E Sbjct: 113 AESYSAANFRQDALREMADIIAAGKIPLLVGGTMLYYKALLEGLSPLPSADEKVRSEIEE 172 Query: 121 EAARVGMPAMHARLAELDPVTAARLKPNDSQRIQRALEISLLTGQPMSALLAQKPAEPLP 180 +A G A+H LA++DP+ A R+ PNDSQRI RALE+ LTG+ +S L+Q+ + LP Sbjct: 173 KAQLQGWAALHQELAKIDPLAAQRINPNDSQRINRALEVFYLTGKSLSE-LSQQKGDSLP 231 Query: 181 MRLLPLALEPSERAVLHDRIALRFDLMLRDGGLEHEVRALRARGDLHLGLPSMRCVGYRQ 240 ++L A+ P +R++LHDRIALRF M+ + G + EV L R DLHL LP+MRCVGYRQ Sbjct: 232 YQILQFAIAPKDRSILHDRIALRFQKMI-EQGFQQEVEKLYQREDLHLDLPAMRCVGYRQ 290 Query: 241 MWEYLDGSIDAAAMREKGIAATRQLAKRQLTWLR 274 MWEYL G D M +GI ATRQLAKRQ+TWLR Sbjct: 291 MWEYLRGDYDHDEMIFRGICATRQLAKRQITWLR 324 >HI0068 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Hin Haemophilus influenzae Length = 311 Score = 318 bits (816), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 161/297 (54%), Positives = 211/297 (71%), Gaps = 3/297 (1%) Query: 1 MGPTASGKTAAALEIARHLPCEIISVDSALVYREMDIGTAKPTPAELAQVQHHLIDIIDP 60 MGPTASGKT A+++ LP E+ISVDSAL+Y+ MDIGTAKP+ ELA H LIDI+DP Sbjct: 9 MGPTASGKTDLAIQLRSQLPVEVISVDSALIYKGMDIGTAKPSKEELALAPHRLIDILDP 68 Query: 61 TEAYSAMQFRQDALRLTQEIIARGRLPLLVGGTMLYFKVLRDGLDELPQADPALRAQLDE 120 +E+YSAM FR DALR +I A+G++PLLVGGTMLY+K L +GL LP AD +RA+L++ Sbjct: 69 SESYSAMNFRDDALREMADITAQGKIPLLVGGTMLYYKALIEGLSPLPSADENIRAELEQ 128 Query: 121 EAARVGMPAMHARLAELDPVTAARLKPNDSQRIQRALEISLLTGQPMSALLAQKPAEPLP 180 +AA+ G A+H LA++DP++AAR+ P+DSQRI RALE+ +TG+ ++ L +K E LP Sbjct: 129 KAAQQGWAALHTELAKIDPISAARINPSDSQRINRALEVFYITGKSLTELTEEK-GEALP 187 Query: 181 MRLLPLALEPSERAVLHDRIALRFDLMLRDGGLEHEVRALRARGDLHLGLPSMRCVGYRQ 240 + A+ P +R VLH+RI RF M+ + G + EV L ARGDL++ LPS+RCVGYRQ Sbjct: 188 YDFVQFAIAPQDRHVLHERIEQRFHKMI-ELGFQAEVEKLYARGDLNINLPSIRCVGYRQ 246 Query: 241 MWEYLDGSIDAAAMREKGIAATRQLAKRQLTWLRSMPERIS-IDCNRPEATAEVLAR 296 MWEYL G M +GI ATRQLAKRQLTWLR I +D +P+ E + R Sbjct: 247 MWEYLQGDYAYEEMIFRGICATRQLAKRQLTWLRGWKTPIQWLDSLQPQQAKETVLR 303 >VC0346 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Vch Vibrio cholerae Length = 315 Score = 317 bits (813), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 164/298 (55%), Positives = 212/298 (71%), Gaps = 6/298 (2%) Query: 1 MGPTASGKTAAALEIARHLPCEIISVDSALVYREMDIGTAKPTPAELAQVQHHLIDIIDP 60 MGPTASGKT A+ + + P EIISVDSAL+YR MDIGTAKP ELA H LIDI+DP Sbjct: 12 MGPTASGKTDLAIRLRQKYPVEIISVDSALIYRGMDIGTAKPDAQELALAPHRLIDILDP 71 Query: 61 TEAYSAMQFRQDALRLTQEIIARGRLPLLVGGTMLYFKVLRDGLDELPQADPALRAQLDE 120 +EAYSA FR+DAL+ +I+A+G++PLLVGGTMLYFK L +GL LP ADP +R Q++ Sbjct: 72 SEAYSAADFRRDALKEMADIVAQGKIPLLVGGTMLYFKALLEGLSPLPAADPVIRQQIEL 131 Query: 121 EAARVGMPAMHARLAELDPVTAARLKPNDSQRIQRALEISLLTGQPMSALLAQKPAEPLP 180 EA ++G A+H +L ++DPV+A R+ PND QR+ RALE+ ++G+ ++ L Q E +P Sbjct: 132 EAEKLGWQALHDQLQQIDPVSAQRIHPNDPQRLSRALEVYRISGKTLTE-LTQTKGEAIP 190 Query: 181 MRLLPLALEPSERAVLHDRIALRFDLMLRDGGLEHEVRALRARGDLHLGLPSMRCVGYRQ 240 R+L A+ P ERA LH RI LRF+ M+ + G E EV+AL AR DLH LPS+RCVGYRQ Sbjct: 191 YRVLQFAIAPKERAELHRRIELRFEKMV-ESGFEEEVKALYARDDLHPDLPSIRCVGYRQ 249 Query: 241 MWEYLD--GSIDAAAMREKGIAATRQLAKRQLTWLRSMPERISIDCNRPEATAEVLAR 296 MW+YLD G++D A R GI ATRQLAKRQ+TWLRS + +D + E L+ Sbjct: 250 MWDYLDGHGTLDEAIYR--GICATRQLAKRQITWLRSWDDLTWLDSENVDQAVETLSN 305 >STM4360 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Sty Salmonella typhimurium LT2 Length = 316 Score = 316 bits (809), Expect = 5e-86, Method: Compositional matrix adjust. Identities = 165/303 (54%), Positives = 209/303 (68%), Gaps = 3/303 (0%) Query: 1 MGPTASGKTAAALEIARHLPCEIISVDSALVYREMDIGTAKPTPAELAQVQHHLIDIIDP 60 MGPTASGKTA A+E+ + LP E+ISVDSAL+YR MDIGTAKP EL H L+DI DP Sbjct: 16 MGPTASGKTALAIELRKVLPVELISVDSALIYRGMDIGTAKPNADELKAAPHRLLDIRDP 75 Query: 61 TEAYSAMQFRQDALRLTQEIIARGRLPLLVGGTMLYFKVLRDGLDELPQADPALRAQLDE 120 ++AYSA FR+DAL EI A GR+PLLVGGTMLYFK L +GL LP ADP +R+++++ Sbjct: 76 SQAYSAADFRRDALAQMAEITAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRSRIEQ 135 Query: 121 EAARVGMPAMHARLAELDPVTAARLKPNDSQRIQRALEISLLTGQPMSALLAQKPAEPLP 180 +AA +G A+H +L E+DPV AAR+ PND QR+ RALE+ ++G+ ++ L Q + LP Sbjct: 136 QAAELGWEALHQQLQEIDPVAAARIHPNDPQRLSRALEVFFISGKTLTE-LTQTSGDALP 194 Query: 181 MRLLPLALEPSERAVLHDRIALRFDLMLRDGGLEHEVRALRARGDLHLGLPSMRCVGYRQ 240 ++ A+ P+ R +LH RI LRF ML G E EVRAL ARGDLH LPS+RCVGYRQ Sbjct: 195 YQVHQFAIAPASRELLHQRIELRFHQMLA-SGFEAEVRALFARGDLHTDLPSIRCVGYRQ 253 Query: 241 MWEYLDGSIDAAAMREKGIAATRQLAKRQLTWLRSMPERISIDCNRPE-ATAEVLARVRE 299 MW Y++G I M +G+ ATRQLAKRQ+TWLR +D P+ A EVL V Sbjct: 254 MWSYIEGEISYDEMVYRGVCATRQLAKRQMTWLRGWEGVRWLDSENPDRARKEVLQVVGA 313 Query: 300 AVD 302 D Sbjct: 314 IAD 316