BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0135.p (244 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5281 [R] COG1011 Predicted hydrolase (HAD superfamily) Pae Pse... 130 3e-30 VC0129 [R] COG1011 Predicted hydrolase (HAD superfamily) Vch Vib... 96 6e-20 STM3950 [R] COG1011 Predicted hydrolase (HAD superfamily) Sty Sa... 93 4e-19 PM1700 [R] COG1011 Predicted hydrolase (HAD superfamily) Pmu Pas... 86 7e-17 ECs4742 [R] COG1011 Predicted hydrolase (HAD superfamily) Ecs Es... 85 1e-16 >PA5281 [R] COG1011 Predicted hydrolase (HAD superfamily) Pae Pseudomonas aeruginosa Length = 232 Score = 130 bits (327), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 82/225 (36%), Positives = 119/225 (52%), Gaps = 7/225 (3%) Query: 15 SPIQAVLFDLDDTLWAIEPVLVRAETLLYDWLRQHAPAVAAAHTIASLRERRLALMQTDP 74 S ++ V FDLDDTLW + PV+ AE LL +WL +A + I L R L+ +P Sbjct: 2 SSLRLVTFDLDDTLWDVAPVMNNAEALLREWLASNAARLGPV-PIEHLWAIRTRLLDREP 60 Query: 75 AYRINLWKLR-----HTALSEVFREHDVDLALVDPAMALFSEARSTVALFDDVEPALLRL 129 R L +LR H L + + + + +L + +F EAR V LF +V P L L Sbjct: 61 MLRHRLSELRRRILFHALLDAGYPQAEAE-SLAEAGFQVFLEARHRVTLFPEVHPTLEIL 119 Query: 130 KGKLTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPGATVY 189 + TLG ++NG AD+ R+GLA +F ++ A G KPDP F A + V A ++ Sbjct: 120 ADRFTLGVLTNGNADVRRLGLADYFRFALCAEELGVGKPDPTPFREALKRAGVEASAAIH 179 Query: 190 VGDDPLLDVQGAQQAGLKAVWMNRFGRVLPEGIRPDAVCRDLHEL 234 +GD P D+ GA++AG++A+W N G+ P A R L EL Sbjct: 180 IGDHPSDDIAGARRAGMRAIWFNPSGKPWAGEEEPSAEIRSLAEL 224 >VC0129 [R] COG1011 Predicted hydrolase (HAD superfamily) Vch Vibrio cholerae Length = 239 Score = 96.3 bits (238), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 69/226 (30%), Positives = 113/226 (50%), Gaps = 9/226 (3%) Query: 17 IQAVLFDLDDTLWAIEPVLVRAETLLYDWLRQHAPAVAAAHTIASLRERR-LALMQTDPA 75 IQA+ FDLDDTL+ PV+ + E + +WL P A L +R +A + Sbjct: 10 IQALTFDLDDTLYDNRPVIKQVEEKVTEWLLSEHPITATRPLAWWLAMKRDIARRFPEQC 69 Query: 76 YRINLWK---LRHTALSEVFREHDVDLALVDPAMALFSEARSTVALFDDVEPALLRLKGK 132 + ++ W+ ++H L + + + + A + + R+ V + + L +L K Sbjct: 70 HDVSQWRYLQVQHGLLELGYAQPEAEQAASE-TLEQVMRWRNQVDVPAETHRVLAQLAAK 128 Query: 133 LTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPGATVYVGD 192 + L +++NG +E+IGL+G+F + A GRAKP P +F A + LQ+ P + ++VGD Sbjct: 129 VPLIAITNGNVQIEKIGLSGYFQTVLRAGPDGRAKPYPDLFAQAAQQLQLEPRSILHVGD 188 Query: 193 DPLLDVQGAQQAGLKAVWMNRFG----RVLPEGIRPDAVCRDLHEL 234 DV GA+Q G +A W N G R+ + PD L EL Sbjct: 189 HLQTDVLGARQNGFQACWFNDQGQSIRRLAKASVLPDVEIERLSEL 234 >STM3950 [R] COG1011 Predicted hydrolase (HAD superfamily) Sty Salmonella typhimurium LT2 Length = 238 Score = 93.2 bits (230), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 60/200 (30%), Positives = 104/200 (52%), Gaps = 5/200 (2%) Query: 17 IQAVLFDLDDTLWAIEPVLVRAETLLYDWLRQHAPAVAAAHTIASLRERRLALMQTDPAY 76 I A+ FDLDDTL+ PV++R E +++ + P++ + + L+ R A+ + +P Sbjct: 10 IAALTFDLDDTLYDNRPVILRTEQEALAFMQNYHPSLRSFQNV-DLQRIRQAVREAEPEI 68 Query: 77 RINLWKLRHTALSEVFREHDVD----LALVDPAMALFSEARSTVALFDDVEPALLRLKGK 132 ++ + RH A+ + R+ + +A + AM F++ RS + + L +L K Sbjct: 69 YHDVTRWRHRAIEQAMRDAGLSAQEAIAGANAAMMHFAKWRSQIEVPQATHETLQQLAKK 128 Query: 133 LTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPGATVYVGD 192 L +++NG A E GL +F + A GR+KP ++ A E L V G ++VGD Sbjct: 129 WPLVAITNGNAQPELFGLGDYFKFVLRAGPDGRSKPFSDMYFLAAEKLHVPIGEILHVGD 188 Query: 193 DPLLDVQGAQQAGLKAVWMN 212 D DV GA + G++A W+ Sbjct: 189 DLTTDVAGAIRCGMQACWIK 208 >PM1700 [R] COG1011 Predicted hydrolase (HAD superfamily) Pmu Pasteurella multocida Length = 236 Score = 85.9 bits (211), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 62/207 (29%), Positives = 103/207 (49%), Gaps = 8/207 (3%) Query: 16 PIQAVLFDLDDTLWAIEPVLVRAETLLYDWLRQHAPAVAAAHTIASLRERRLALMQTDPA 75 P + + FDLDDTL+ V+ AE + ++ + ++ + ++R + Q P Sbjct: 9 PFKLISFDLDDTLYDNSDVIRLAEENFIEKVKLESQLNISSEEWRAWKQR---IEQRHPM 65 Query: 76 YRINLWKLRHTALSEVFREHDVDLALVD----PAMALFSEARSTVALFDDVEPALLRLKG 131 ++ R L ++ + A ++ AMALF E R + + + L LK Sbjct: 66 LCEDVVAWRIETLHQLLANYQKSAAEIERVCQQAMALFVEWRHKIDVPVQSQQVLNLLKQ 125 Query: 132 KLTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPGATVYVG 191 K L +++NG + +RIGL+ F + + GRAKP +FH + V P ++VG Sbjct: 126 KYPLVAITNGNVEPQRIGLS-QFDLVLRGGEQGRAKPHQDLFHQTAQHFGVQPHEILHVG 184 Query: 192 DDPLLDVQGAQQAGLKAVWMNRFGRVL 218 D+ + DVQGA QA +AVW+N G+VL Sbjct: 185 DNLMTDVQGAIQADCQAVWINLSGKVL 211 >ECs4742 [R] COG1011 Predicted hydrolase (HAD superfamily) Ecs Escherichia coli O157:H7 Length = 238 Score = 85.1 bits (209), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 60/199 (30%), Positives = 100/199 (50%), Gaps = 5/199 (2%) Query: 17 IQAVLFDLDDTLWAIEPVLVRAETLLYDWLRQHAPAVAAAHTIASLRERRLALMQTDPA- 75 I A+ FDLDDTL+ PV++R E +++ + PA+ + L+ R A+ + +P Sbjct: 10 ISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHPALRSFQN-EDLQRLRQAVREAEPEI 68 Query: 76 -YRINLWKLR--HTALSEVFREHDVDLALVDPAMALFSEARSTVALFDDVEPALLRLKGK 132 + + W+ R A+ + + A AM F++ RS + + L +L K Sbjct: 69 YHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAMINFAKWRSRIDVPQQTHDTLKQLAKK 128 Query: 133 LTLGSVSNGFADLERIGLAGHFGVSIAAHRFGRAKPDPAIFHAACEALQVAPGATVYVGD 192 L +++NG A E GL +F + A GR+KP ++ A E L V G ++VGD Sbjct: 129 WPLVAITNGNAQPELFGLGDYFEFVLRAGPHGRSKPFSDMYFLAAEKLNVPIGEILHVGD 188 Query: 193 DPLLDVQGAQQAGLKAVWM 211 D DV GA ++G++A W+ Sbjct: 189 DLTTDVGGAIRSGMQACWI 207