BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0025.p (238 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 262 4e-70 slr0541 [F] COG0518 GMP synthase - Glutamine amidotransferase do... 141 2e-33 TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 130 2e-30 PA1729 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 118 1e-26 MA2696 [F] COG0518 GMP synthase - Glutamine amidotransferase dom... 117 3e-26 >RSc1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Rso Ralstonia solanacearum Length = 242 Score = 262 bits (670), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 139/232 (59%), Positives = 169/232 (72%), Gaps = 3/232 (1%) Query: 1 MKEAIALRHVHFEDVGILDSLLASRGYRLRYIDATIEQADTAAAEQVDLLIVLGGPIGAY 60 MK A+ALRHV FE +G+L+SLL RGY +R +DA ++ A DL++VLGGPIGA+ Sbjct: 1 MKTALALRHVPFEHLGVLESLLLGRGYAVRTLDAGLQPLPAEALAAADLVVVLGGPIGAF 60 Query: 61 DEGLHPFLLRELDLIARRLQQQRPILGICLGAQLIARALGAVVAPMG--LKEIGYAPLDL 118 D+ +PFL E D IARRL ++P+LGICLGAQL+ARALGA VAPMG +KEIG+APL L Sbjct: 61 DDDRYPFLREETDAIARRLAARKPLLGICLGAQLMARALGAAVAPMGDNVKEIGFAPLTL 120 Query: 119 STEGSLSPLAAL-EGQPVLHWHGDQFAIPPGATCLASTPVCPHQAFSLGAHVLALQFHLE 177 + EG+ SPLA L +G PVLHWHGD+F +PPGAT LAST VC QAF+LG H L LQFHLE Sbjct: 121 TAEGAASPLAPLADGTPVLHWHGDRFELPPGATRLASTSVCAEQAFALGDHALGLQFHLE 180 Query: 178 ADPARINQWLVGHACELGQAGIDPRSLREQAKRFGTDLRSAAHAVMSNWLDK 229 AD + WL+GHA EL AGIDPR+LR QA L AH V + WLD+ Sbjct: 181 ADLRELEAWLIGHAAELAAAGIDPRTLRGQAPALAAPLAQRAHDVFTAWLDR 232 >slr0541 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Syn Synechocystis Length = 236 Score = 141 bits (355), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 75/179 (41%), Positives = 106/179 (59%), Gaps = 7/179 (3%) Query: 7 LRHVHFEDVGILDSLLASRGYRLRYIDATIEQADTAAAEQVDLLIVLGGPIGAYDEGLHP 66 +HV FE++G ++ L ++GY + + + +DLLIVLGGP+ DE +P Sbjct: 6 FQHVPFENLGAIERWLRAKGYAISQTAFFQPSFSLPSLDAIDLLIVLGGPMSVNDEAQYP 65 Query: 67 FLLRELDLIARRLQQQRPILGICLGAQLIARALGAVVAPMGLKEIGYAPL----DLSTEG 122 +L++E + I + + +PILGICLGAQL+A ALGA V P +KEIG+ P+ S G Sbjct: 66 WLVQEKEFIRQAIAVGKPILGICLGAQLMANALGAKVYPNAVKEIGWFPIMGQEGKSQSG 125 Query: 123 SLSPLAALEGQPVLHWHGDQFAIPPGATCLASTPVCPHQAFSLGAHVLALQFHLEADPA 181 S +LE V HWHG+ F +PPGA +AS+ C HQAF +G + LQ HLE P Sbjct: 126 SFQFPPSLE---VFHWHGETFDLPPGAELIASSQACQHQAFQIGRSAIGLQCHLETTPT 181 >TM1158 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Tma Thermotoga maritima Length = 227 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 70/208 (33%), Positives = 114/208 (54%), Gaps = 3/208 (1%) Query: 4 AIALRHVHFEDVGILDSLLASRGYRLRYIDATIEQADTAAAEQVDLLIVLGGPIGAYDEG 63 +A+RHV ED+G+++ + + + Y+D + E+ L+++LGG +GAY+E Sbjct: 3 VLAIRHVEIEDLGMMEDIFREKNWSFDYLDTPKGEKLERPLEEYSLVVLLGGYMGAYEEE 62 Query: 64 LHPFLLRELDLIARRLQQQRPILGICLGAQLIARALGA-VVAPMGLKEIGYAPLDLSTEG 122 +PFL E LI L+++ P LGICLG+Q++A+ LGA V +EIG+ ++ ++ Sbjct: 63 KYPFLKYEFQLIEEILKKEIPFLGICLGSQMLAKVLGASVYRGKNGEEIGWYFVEKVSDN 122 Query: 123 SLSPLAALEGQPVLHWHGDQFAIPPGATCLASTPVCPHQAFSLGAHVLALQFHLEADPAR 182 + V WHGD F +P AT + ++ +Q F G V LQFH+E Sbjct: 123 KFFREFP-DRLRVFQWHGDTFDLPRRATRVFTSEKYENQGFVYGKAV-GLQFHIEVGART 180 Query: 183 INQWLVGHACELGQAGIDPRSLREQAKR 210 + +W+ + EL + IDPR L E A+R Sbjct: 181 MKRWIEAYKDELEKKKIDPRLLLETAER 208 >PA1729 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Pae Pseudomonas aeruginosa Length = 215 Score = 118 bits (295), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 69/189 (36%), Positives = 101/189 (53%), Gaps = 2/189 (1%) Query: 1 MKEAIALRHVHFEDVGILDSLLASRGYRLRYIDATIEQADTAAAEQVDLLIVLGGPIGAY 60 M + + L H F G L ++LA RG R I A + + AE+ + ++GGP+ Sbjct: 1 MSDILILTHAEFCPPGHLGAVLAERGLDFRVIRADLGELAGLDAERPRAVAIMGGPMSVN 60 Query: 61 DEGLHPFLLRELDLIARRLQQQRPILGICLGAQLIARALGAVVAPMGLKEIGYAPLDLST 120 D+ P+L EL L+ R ++++ P++G CLG QL+ARALGA V E+G+ P+ + Sbjct: 61 DD--LPWLRDELALLRRFIERRIPLIGHCLGGQLLARALGATVRHQPYTEMGWQPMQRES 118 Query: 121 EGSLSPLAALEGQPVLHWHGDQFAIPPGATCLASTPVCPHQAFSLGAHVLALQFHLEADP 180 S E + WHGD F +P GA L S+P C +Q FS G HVL LQ H E Sbjct: 119 RDSPWLAGLPERFSIFQWHGDAFDLPQGAERLLSSPWCENQGFSWGGHVLGLQGHPEMTE 178 Query: 181 ARINQWLVG 189 + +W+ G Sbjct: 179 ELVRRWIAG 187 >MA2696 [F] COG0518 GMP synthase - Glutamine amidotransferase domain Mac Methanosarcina acetivorans str.C2A Length = 238 Score = 117 bits (292), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 73/231 (31%), Positives = 109/231 (47%), Gaps = 9/231 (3%) Query: 6 ALRHVHFEDVGILDSLLASRGYRLRYIDATIEQADTAAAEQVDLLIVLGGPIGAYDEGLH 65 L+H +G ++ +R Y L E + + + DLLI++GGP+G YD + Sbjct: 5 VLQHSALNTLGTIEEYAKTRSYPLEST-RFYETKNPPSLDSFDLLIIMGGPMGIYDYAEN 63 Query: 66 PFLLRELDLIARRLQQQRPILGICLGAQLIARALGAVVAPMGLKEIGYAPLDLSTEGSLS 125 P+L E I + + +P+LGICLGAQL+A LGA + G +E+G+ P+ + Sbjct: 64 PWLRDEKSFIKQAIDAGKPVLGICLGAQLLANILGARIYENGHREMGWFPVKAVRKEENK 123 Query: 126 PLAALEGQP----VLHWHGDQFAIPPGATCLASTPVCPHQAFSLGAHVLALQFHLEADPA 181 P L+G P V HWH F +P GA L + C +Q F G V+ALQFH E Sbjct: 124 P-EFLKGLPEEITVFHWHSRTFDLPEGAVHLFRSEGCKNQGFIYGGRVVALQFHPEVHEE 182 Query: 182 RINQWLVGHACELGQAGIDPRSLREQAKRFGTDLRSAAHAVMSNWLDKLEA 232 R+ + G++G P + R++ M LDK E Sbjct: 183 RVESMIRRFG---GESGNGPFTQRKEEMVGQEKYLVETKKFMFAVLDKFEK 230