BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0517.p (326 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC320.14 [E] COG1171 Threonine dehydratase Spo Schizosaccharom... 441 e-124 PA2683 [E] COG1171 Threonine dehydratase Pae Pseudomonas aeruginosa 426 e-119 RSp1516 [E] COG1171 Threonine dehydratase Rso Ralstonia solanace... 423 e-118 YKL218c [E] COG1171 Threonine dehydratase Sce Saccharomyces cere... 422 e-118 CC3105 [E] COG1171 Threonine dehydratase Ccr Caulobacter vibrioides 280 2e-75 >SPCC320.14 [E] COG1171 Threonine dehydratase Spo Schizosaccharomyces pombe Length = 323 Score = 441 bits (1135), Expect = e-124, Method: Compositional matrix adjust. Identities = 212/316 (67%), Positives = 263/316 (83%) Query: 10 LPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALT 69 LP++ DV A+ERIK +A++TPVL S T + E A++FFKCENFQ+MGAFKFRGA+NAL+ Sbjct: 7 LPTYDDVASASERIKKFANKTPVLTSSTVNKEFVAEVFFKCENFQKMGAFKFRGALNALS 66 Query: 70 QFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTY 129 Q + QRK GV+TFSSGNHAQ AL+A++LGIPA I+MP DAP AKVAAT+GYG +V+ Y Sbjct: 67 QLNEAQRKAGVLTFSSGNHAQAIALSAKILGIPAKIIMPLDAPEAKVAATKGYGGQVIMY 126 Query: 130 DRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGGG 189 DRY +DRE + ++ +T+IPPYDHPHV+AGQGTA EL +E GPLDALFVCLGGGG Sbjct: 127 DRYKDDREKMAKEISEREGLTIIPPYDHPHVLAGQGTAAKELFEEVGPLDALFVCLGGGG 186 Query: 190 LLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKYT 249 LLSG+ALAAR +P C++YGVEP+AGNDGQQS R G +VHIDTPKTIADGAQTQHLG YT Sbjct: 187 LLSGSALAARHFAPNCEVYGVEPEAGNDGQQSFRKGSIVHIDTPKTIADGAQTQHLGNYT 246 Query: 250 FGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGVI 309 F +I+E VDDI TVSDEEL+ ++F+A RMK+VVEPTGCL FAAAR +K++L+ KR+G+I Sbjct: 247 FSIIKEKVDDILTVSDEELIDCLKFYAARMKIVVEPTGCLSFAAARAMKEKLKNKRIGII 306 Query: 310 ISGGNVDLGKLATFLA 325 ISGGNVD+ + A FL+ Sbjct: 307 ISGGNVDIERYAHFLS 322 >PA2683 [E] COG1171 Threonine dehydratase Pae Pseudomonas aeruginosa Length = 320 Score = 426 bits (1095), Expect = e-119, Method: Compositional matrix adjust. Identities = 219/316 (69%), Positives = 256/316 (81%) Query: 9 ELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNAL 68 +LP++ DV+ AA RI A+RTPV+ SRT D ELGA++FFKCEN QRMGAFKFRGA NAL Sbjct: 3 DLPTYDDVIAAAARIAGHANRTPVMSSRTLDEELGAEVFFKCENLQRMGAFKFRGAFNAL 62 Query: 69 TQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVT 128 ++F A QR GV+ FSSGNHAQ AL+A+LLGIPA IVMP DAPA K+ ATRGYG +VV Sbjct: 63 SRFSAEQRAAGVVAFSSGNHAQAIALSARLLGIPATIVMPADAPAVKIEATRGYGGQVVL 122 Query: 129 YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 YDRYTEDRE IG LA +TLIPPYDHP V+AGQGTA EL +E GPLDA F LGGG Sbjct: 123 YDRYTEDREQIGRDLAQRHGLTLIPPYDHPDVLAGQGTAAKELFEEVGPLDAFFAPLGGG 182 Query: 189 GLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKY 248 GLLSG ALA RAL+PAC+IYGVEP+AGNDGQ+SLR+G +VHIDTP+T+ADGAQTQHLG Sbjct: 183 GLLSGCALAIRALAPACRIYGVEPEAGNDGQRSLRSGAIVHIDTPQTLADGAQTQHLGNL 242 Query: 249 TFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGV 308 TF +I+ VDDI T SD ELV GMRF A RMK++VEPTGCLG AAAR+ KD+L+GKRVG+ Sbjct: 243 TFPLIQRNVDDILTASDAELVDGMRFLAARMKLLVEPTGCLGLAAARQRKDELRGKRVGI 302 Query: 309 IISGGNVDLGKLATFL 324 ++SGGNVDL + L Sbjct: 303 LLSGGNVDLARFCALL 318 >RSp1516 [E] COG1171 Threonine dehydratase Rso Ralstonia solanacearum Length = 323 Score = 423 bits (1088), Expect = e-118, Method: Compositional matrix adjust. Identities = 232/318 (72%), Positives = 260/318 (81%), Gaps = 1/318 (0%) Query: 8 TELP-SFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMN 66 T LP ++ DV+ A R++ HRTPVL S TA+ GA+LFFKCENFQRMGAFKFRGA N Sbjct: 2 TTLPITYDDVVAAHARLQGQVHRTPVLTSTTANALTGAELFFKCENFQRMGAFKFRGAYN 61 Query: 67 ALTQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEV 126 AL+QF QRK GVITFSSGNHAQ AL+A+LLG+ AVIVMP+DAP KV ATRGYG EV Sbjct: 62 ALSQFTPEQRKAGVITFSSGNHAQAIALSARLLGMRAVIVMPKDAPTIKVEATRGYGGEV 121 Query: 127 VTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLG 186 V YDRYTEDREAIG RLA + +TLIPPYDHPHVMAGQGTA EL++ETGPLD L VCLG Sbjct: 122 VFYDRYTEDREAIGRRLAEQHGLTLIPPYDHPHVMAGQGTAAKELIEETGPLDLLLVCLG 181 Query: 187 GGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLG 246 GGGLLSG A AARALSPAC+I GVEP+AGNDGQQSLR G++VHIDTP TIADGAQTQHLG Sbjct: 182 GGGLLSGCATAARALSPACRIIGVEPEAGNDGQQSLRKGEIVHIDTPATIADGAQTQHLG 241 Query: 247 KYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRV 306 YTF VIR LVDDI TVSD +LV MRFFA RMK+VVEPTGCL AAA + + +++GKRV Sbjct: 242 HYTFAVIRALVDDIATVSDADLVDTMRFFAGRMKIVVEPTGCLAAAAALRQRVEVRGKRV 301 Query: 307 GVIISGGNVDLGKLATFL 324 GVIISGGNVDL A + Sbjct: 302 GVIISGGNVDLQHFARLV 319 >YKL218c [E] COG1171 Threonine dehydratase Sce Saccharomyces cerevisiae Length = 326 Score = 422 bits (1085), Expect = e-118, Method: Compositional matrix adjust. Identities = 213/316 (67%), Positives = 259/316 (81%) Query: 10 LPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALT 69 +P++ DVL A+ RIK + ++TPVL SR + LGAQ++FK ENFQR+GAFKFRGAMNA++ Sbjct: 3 VPTYGDVLDASNRIKEYVNKTPVLTSRMLNDRLGAQIYFKGENFQRVGAFKFRGAMNAVS 62 Query: 70 QFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTY 129 + +R GVI FSSGNHAQ AL+A+LL +PA IVMP+DAPA KVAAT GYGA ++ Y Sbjct: 63 KLSDEKRSKGVIAFSSGNHAQAIALSAKLLNVPATIVMPEDAPALKVAATAGYGAHIIRY 122 Query: 130 DRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGGG 189 +RYTEDRE IG +LAAE LIPPYDHP V+AGQGT+ ELL+E G LDALFV LGGGG Sbjct: 123 NRYTEDREQIGRQLAAEHGFALIPPYDHPDVIAGQGTSAKELLEEVGQLDALFVPLGGGG 182 Query: 190 LLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKYT 249 LLSG+ALAAR+LSP CKI+GVEP+AGNDGQQS R+G +VHI+TPKTIADGAQTQHLG+YT Sbjct: 183 LLSGSALAARSLSPGCKIFGVEPEAGNDGQQSFRSGSIVHINTPKTIADGAQTQHLGEYT 242 Query: 250 FGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGVI 309 F +IRE VDDI TVSD+ELV M F AERMK+VVEPT CLGFA A K++L GK+VG+I Sbjct: 243 FAIIRENVDDILTVSDQELVKCMHFLAERMKVVVEPTACLGFAGALLKKEELVGKKVGII 302 Query: 310 ISGGNVDLGKLATFLA 325 +SGGNVD+ + AT ++ Sbjct: 303 LSGGNVDMKRYATLIS 318 >CC3105 [E] COG1171 Threonine dehydratase Ccr Caulobacter vibrioides Length = 325 Score = 280 bits (717), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 145/318 (45%), Positives = 205/318 (64%), Gaps = 5/318 (1%) Query: 12 SFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALTQF 71 S AD+ AA R+K A TP++ S + LG ++F K E QR GAFKFRGA N L+Q Sbjct: 4 SLADIQAAAVRLKGSAVETPLIESPALNDRLGGRIFLKPETLQRAGAFKFRGAYNRLSQL 63 Query: 72 DATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTYDR 131 ++ GV+ FSSGNHAQG ALAA+LLG+PA+IVMP D+P+ KV TRG+GA++ YDR Sbjct: 64 SDEEKARGVVAFSSGNHAQGVALAARLLGVPALIVMPSDSPSVKVEGTRGFGADIRFYDR 123 Query: 132 YTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQET----GPLDALFVCLGG 187 +TEDR AI ++AAER ++P YD PH++AGQGT LE++ + LD L C+GG Sbjct: 124 FTEDRVAIADQIAAERGCVVVPSYDDPHIIAGQGTVGLEIVAQAAAQGATLDRLICCVGG 183 Query: 188 GGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDT-PKTIADGAQTQHLG 246 GGL++G + A +ALSPA +I+GVEP ++ ++SL +G+ ID ++I D T G Sbjct: 184 GGLIAGTSTAVKALSPATEIWGVEPAGFDETRRSLESGRRETIDKDARSICDALLTPIPG 243 Query: 247 KYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRV 306 T+ + ++ + + V+D E+ MR+ +K+VVEP GC+ AA K + GK V Sbjct: 244 DLTWPINQKNLSGVVAVTDAEVAEAMRYAFSTLKLVVEPGGCVALTAALTGKVDVAGKTV 303 Query: 307 GVIISGGNVDLGKLATFL 324 +++SGGNVD G A L Sbjct: 304 AIVLSGGNVDPGLFAQVL 321