BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0389.p (207 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2826 [NU] COG1459 Type II secretory pathway, component PulF R... 195 6e-50 PA4527 [NU] COG1459 Type II secretory pathway, component PulF Pa... 190 2e-48 XF2538 [NU] COG1459 Type II secretory pathway, component PulF Xf... 181 1e-45 aq_747 [NU] COG1459 Type II secretory pathway, component PulF Aa... 158 8e-39 all2442 [NU] COG1459 Type II secretory pathway, component PulF N... 155 5e-38 >RSc2826 [NU] COG1459 Type II secretory pathway, component PulF Rso Ralstonia solanacearum Length = 421 Score = 195 bits (496), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 90/207 (43%), Positives = 145/207 (70%) Query: 1 MFRHFGAELPLPTRIVMSLAHALTQAWPWLLPGLAIAVLAGLQAWRRWLPLRQYAQRLSL 60 +F FGA LP PT +VM ++ + W ++ + + ++A++R +++ + L Sbjct: 215 VFASFGATLPAPTLVVMGISDFFVKWWYIIIFTPIVGIFLYVRAFKRSEKVQRNTHKAIL 274 Query: 61 RLPLLGGLLRQAALARWSRTFGTLLGAGIALVQALETVAGAAGNVVIGQASLAVRDAVRN 120 ++P+ G ++R+A +ARW+RT T+ AG LV+++E+VAGAAGN V A++ ++ AVR Sbjct: 275 KMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWVYYDATMEIQQAVRI 334 Query: 121 GASLHAAMQATGVFPDLAVQMTAVGEETGTLDAMLIKIAELNEREVDDTVTALTSLMEPA 180 G SL AMQAT VF ++ +QMT +GEE+G LD ML+K+AE EREVDD V +++L+EP Sbjct: 335 GTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPL 394 Query: 181 IMTVLGLVIGGLVIALYLPVFRMGSVV 207 I+ +LG++IGG+V+A+YLP+F++G VV Sbjct: 395 IIVILGVLIGGMVVAMYLPIFKLGQVV 421 >PA4527 [NU] COG1459 Type II secretory pathway, component PulF Pae Pseudomonas aeruginosa Length = 374 Score = 190 bits (483), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 90/207 (43%), Positives = 141/207 (68%) Query: 1 MFRHFGAELPLPTRIVMSLAHALTQAWPWLLPGLAIAVLAGLQAWRRWLPLRQYAQRLSL 60 +F+ FGAELP T++V++L+ L + W ++ G+ + +R R R L Sbjct: 167 VFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFKELHKRSKKFRDTLDRTIL 226 Query: 61 RLPLLGGLLRQAALARWSRTFGTLLGAGIALVQALETVAGAAGNVVIGQASLAVRDAVRN 120 +LP+ GG++ ++A+AR++RT T AG+ LV AL++V+GA GN+V A ++ V Sbjct: 227 KLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVST 286 Query: 121 GASLHAAMQATGVFPDLAVQMTAVGEETGTLDAMLIKIAELNEREVDDTVTALTSLMEPA 180 G L+ +M+ T VFP++A+QMTA+GEE+G+LD ML K+A E EVD+ V LT+LMEP Sbjct: 287 GMQLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPM 346 Query: 181 IMTVLGLVIGGLVIALYLPVFRMGSVV 207 IM VLG+++GGL++A+YLP+F++G+VV Sbjct: 347 IMAVLGVLVGGLIVAMYLPIFQLGNVV 373 >XF2538 [NU] COG1459 Type II secretory pathway, component PulF Xfa Xylella fastidiosa 9a5c Length = 490 Score = 181 bits (459), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 91/208 (43%), Positives = 138/208 (66%), Gaps = 2/208 (0%) Query: 1 MFRHFGAELPLPTRIVMSLAHALTQAWPWLLPGLAIAVLAG-LQAWRRWLPLRQYAQRLS 59 +F+ FGAELP T+++++ ++ Q W W + L I V+ G L ++R ++ RL Sbjct: 283 VFKGFGAELPAFTQMIVNFSN-FMQRWWWAMLALLIVVIGGGLFTYKRSESMQHLLDRLV 341 Query: 60 LRLPLLGGLLRQAALARWSRTFGTLLGAGIALVQALETVAGAAGNVVIGQASLAVRDAVR 119 L+ P++G ++ +ALAR+SRT AG+ LV+AL VAGA GN V A +RD V Sbjct: 342 LKFPVIGAIMHNSALARFSRTTAVTFRAGVPLVEALGIVAGATGNKVYSDAVFRMRDDVS 401 Query: 120 NGASLHAAMQATGVFPDLAVQMTAVGEETGTLDAMLIKIAELNEREVDDTVTALTSLMEP 179 G S++ +M+ G+FP + VQM ++GEE G LDAML K+AE E+EV + V L+SL+EP Sbjct: 402 VGYSINMSMRQVGIFPHMVVQMASIGEEAGALDAMLFKVAEYYEQEVSNAVDGLSSLLEP 461 Query: 180 AIMTVLGLVIGGLVIALYLPVFRMGSVV 207 IM +G+++GG+VIA+YLP+F++ SVV Sbjct: 462 LIMVFIGVIVGGMVIAMYLPIFKLASVV 489 >aq_747 [NU] COG1459 Type II secretory pathway, component PulF Aae Aquifex aeolicus Length = 408 Score = 158 bits (399), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 79/207 (38%), Positives = 125/207 (60%) Query: 1 MFRHFGAELPLPTRIVMSLAHALTQAWPWLLPGLAIAVLAGLQAWRRWLPLRQYAQRLSL 60 +++ G LP PT++++ ++ L + P++L L I+V L +R L R R+ L Sbjct: 200 IYKSLGGTLPAPTQLLIDISLTLQRNIPYILGTLLISVAVFLYFYRTNLKFRALIHRILL 259 Query: 61 RLPLLGGLLRQAALARWSRTFGTLLGAGIALVQALETVAGAAGNVVIGQASLAVRDAVRN 120 RLP+ GG+ ++ A AR++RT TL +G+ L + LE G + I +A R V Sbjct: 260 RLPVFGGIFKKGAFARFARTMATLFASGVPLERVLEISGRVVGFIPIEKALNEARKEVLE 319 Query: 121 GASLHAAMQATGVFPDLAVQMTAVGEETGTLDAMLIKIAELNEREVDDTVTALTSLMEPA 180 G +L A+Q T +FP + + M VGEETG +D ML IA E EVD ++ L +L+EPA Sbjct: 320 GKNLWMALQNTEMFPKMIIAMVRVGEETGQMDQMLNSIANFYEEEVDRSIEGLVALIEPA 379 Query: 181 IMTVLGLVIGGLVIALYLPVFRMGSVV 207 ++ +LG++IG ++IALYLP+F +GS + Sbjct: 380 LIVILGVIIGAILIALYLPIFNIGSFI 406 Score = 63.9 bits (154), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 33/108 (30%), Positives = 59/108 (54%), Gaps = 1/108 (0%) Query: 58 LSLRLPLLGGLLRQAALARWSRTFGTLLGAGIALVQALETVAGAAGNVVIGQASLAVRDA 117 + +PL G + + + R L+GAG+++V+A+E VA N + +A + Sbjct: 53 FEINIPLPFGSVSERDWSLLCRQLSVLVGAGVSIVEAIELVAEQMQNKKLRKALMETAKD 112 Query: 118 VRNGASLHAAM-QATGVFPDLAVQMTAVGEETGTLDAMLIKIAELNER 164 + G+S+H A+ + +FP+ + + VGEETG LD +L + AE E+ Sbjct: 113 IEEGSSIHDALAKRKNIFPEFLINLVEVGEETGELDLVLRRAAEYYEK 160 >all2442 [NU] COG1459 Type II secretory pathway, component PulF Nos Nostoc sp. PCC 7120 Length = 407 Score = 155 bits (392), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 80/207 (38%), Positives = 133/207 (64%), Gaps = 8/207 (3%) Query: 1 MFRHFGAELPLPTRIVMSLAHALTQAWPWLLPGLAIAVLAGLQ-AWRRWLPLR---QYAQ 56 +F G ELP T +M+ + L W ++ IA + G + A+ ++ R + Sbjct: 200 IFVELGTELPPLTAFLMTCSQVLRSLWSLVI----IAGIVGFKFAYSQYYKTRIGRETID 255 Query: 57 RLSLRLPLLGGLLRQAALARWSRTFGTLLGAGIALVQALETVAGAAGNVVIGQASLAVRD 116 RLSL++PL G L++++++AR+SRTFG+L +G+ ++ +LE V +GN V+ A A R Sbjct: 256 RLSLKMPLFGDLIQKSSVARFSRTFGSLTRSGVPILTSLEIVRDTSGNQVVANAIDAARA 315 Query: 117 AVRNGASLHAAMQATGVFPDLAVQMTAVGEETGTLDAMLIKIAELNEREVDDTVTALTSL 176 ++ G + A+Q VFP +A+QM ++GEETG LD ML+KIA+ E EV+ V ALTS+ Sbjct: 316 EIQQGGMISIALQKEAVFPPMAIQMISIGEETGELDGMLMKIADFYEDEVEQAVKALTSV 375 Query: 177 MEPAIMTVLGLVIGGLVIALYLPVFRM 203 +EP ++ VLG ++G +++A+YLP+F++ Sbjct: 376 LEPIMILVLGGMVGTILLAMYLPLFKV 402