BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0378.p (372 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2819 [E] COG0263 Glutamate 5-kinase Rso Ralstonia solanacearum 511 e-145 PA4565 [E] COG0263 Glutamate 5-kinase Pae Pseudomonas aeruginosa 450 e-126 DR1827 [E] COG0263 Glutamate 5-kinase Dra Deinococcus radiodurans 297 2e-80 STM0321 [E] COG0263 Glutamate 5-kinase Sty Salmonella typhimuriu... 296 5e-80 proB [E] COG0263 Glutamate 5-kinase Eco Escherichia coli K12 295 1e-79 >RSc2819 [E] COG0263 Glutamate 5-kinase Rso Ralstonia solanacearum Length = 374 Score = 511 bits (1317), Expect = e-145, Method: Compositional matrix adjust. Identities = 270/372 (72%), Positives = 310/372 (83%) Query: 1 MQSVIQRAKRLIIKVGSSLVTNDGKGLDANAIARWAEQIAQLRALGKEVVLVSSGAVAEG 60 ++S+I A+RL++KVGSSLVT+DG+GLD AIARWA QIA LRA GKEVVLVSSGA+AEG Sbjct: 3 LRSLIADARRLVVKVGSSLVTDDGRGLDQAAIARWAAQIAALRAAGKEVVLVSSGAIAEG 62 Query: 61 MQRLGFEKRPTGIHHLQACAAVGQMGLAQIYETSFRKHDLHTAQVLLTHADLADRERYLN 120 MQRLG+ +RP IH LQA AAVGQMGLAQ+YE+ F +H + TAQVLLTH DLADRERYLN Sbjct: 63 MQRLGWTRRPKEIHELQAAAAVGQMGLAQVYESEFARHGIRTAQVLLTHGDLADRERYLN 122 Query: 121 ARSTLFALLQLGVVPVINENDTVVTDEIKFGDNDTLGALVANLIEAEALIILTDQPGLFT 180 ARSTL LL LGVVP+INENDTVVTDEIKFGDNDTLGALV NLIE +ALIILTDQ GL+T Sbjct: 123 ARSTLLTLLGLGVVPIINENDTVVTDEIKFGDNDTLGALVTNLIEGDALIILTDQRGLYT 182 Query: 181 ADPRKDPTATLISEARAGDPSLELIAGGTGTGIGRGGMLTKILAAKRAATSGAHTVIAWG 240 ADPRK P A + EA+AG P LE +AGG G+ IG+GGMLTKI+AAKRAA SGAHTVIA G Sbjct: 183 ADPRKHPDARFVDEAQAGAPELEAMAGGAGSSIGKGGMLTKIVAAKRAAKSGAHTVIASG 242 Query: 241 REEQVLTRLAAGEAIGTQLNAETAQLTARKQWMADHLKTAGRVVLDAGAVQKLSLEGKSL 300 RE VL RLA GEAIGTQL A T ++ ARKQWM DHL+ GRVVLDAGAV KL+ GKSL Sbjct: 243 READVLARLAGGEAIGTQLRAPTGRMAARKQWMIDHLQLRGRVVLDAGAVDKLTAGGKSL 302 Query: 301 LPIGVVEVSGEFGRGDVITCVDENGRAIARGMSNYNSSDARRIIRHPSSEIQAILGFVEE 360 LPIGV EV GEF RG+VI+CVD GR +ARG++NY++++AR I R SSEI+A+LG+V Sbjct: 303 LPIGVTEVQGEFARGEVISCVDTAGREVARGLTNYSAAEARLIARKASSEIEAVLGYVSA 362 Query: 361 PELVHRDNLVLL 372 ELVHRDNLVLL Sbjct: 363 AELVHRDNLVLL 374 >PA4565 [E] COG0263 Glutamate 5-kinase Pae Pseudomonas aeruginosa Length = 372 Score = 450 bits (1158), Expect = e-126, Method: Compositional matrix adjust. Identities = 222/372 (59%), Positives = 287/372 (77%) Query: 1 MQSVIQRAKRLIIKVGSSLVTNDGKGLDANAIARWAEQIAQLRALGKEVVLVSSGAVAEG 60 M+ + A+R ++K+GS+L+T DG+GLD NA+A W EQ+ L G E+VLVSSGAVA G Sbjct: 1 MRDKVTGARRWVVKIGSALLTADGRGLDRNAMAVWVEQMVALHCAGIELVLVSSGAVAAG 60 Query: 61 MQRLGFEKRPTGIHHLQACAAVGQMGLAQIYETSFRKHDLHTAQVLLTHADLADRERYLN 120 M RLG+ RP+ +H LQA A+VGQMGL Q +E+SF H L TAQVLLTH DL+DR+RYLN Sbjct: 61 MSRLGWVSRPSAMHELQAAASVGQMGLVQAWESSFALHGLQTAQVLLTHDDLSDRKRYLN 120 Query: 121 ARSTLFALLQLGVVPVINENDTVVTDEIKFGDNDTLGALVANLIEAEALIILTDQPGLFT 180 ARSTL L++LGVVPVINENDTVVTDEI+FGDNDTL ALVANL+EA+ L+ILTD+ G+F Sbjct: 121 ARSTLRTLVELGVVPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFD 180 Query: 181 ADPRKDPTATLISEARAGDPSLELIAGGTGTGIGRGGMLTKILAAKRAATSGAHTVIAWG 240 ADPR +P A LI EARA DP L+ +AGG+ +GRGGM TK+ AA+ AA SG HTVI G Sbjct: 181 ADPRNNPDAQLIYEARADDPQLDAVAGGSAGALGRGGMQTKLRAARLAARSGGHTVIVGG 240 Query: 241 REEQVLTRLAAGEAIGTQLNAETAQLTARKQWMADHLKTAGRVVLDAGAVQKLSLEGKSL 300 R E+VL RL AGE +GT L + ++ ARKQW+A HL+ G +VLD GAV+ +S + KSL Sbjct: 241 RIERVLDRLRAGERLGTLLTPDRSRKAARKQWLAGHLQMRGTLVLDDGAVKAVSQDHKSL 300 Query: 301 LPIGVVEVSGEFGRGDVITCVDENGRAIARGMSNYNSSDARRIIRHPSSEIQAILGFVEE 360 LP+GV V G F RG+++ CVD+ GR +ARG+ NY++ +A++I+ P+ I+A+LG+V+ Sbjct: 301 LPVGVKAVQGSFRRGEMVVCVDQGGREVARGLVNYSALEAQKILGQPTDAIEALLGYVDG 360 Query: 361 PELVHRDNLVLL 372 PELVHRDNLVL+ Sbjct: 361 PELVHRDNLVLV 372 >DR1827 [E] COG0263 Glutamate 5-kinase Dra Deinococcus radiodurans Length = 363 Score = 297 bits (761), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 168/363 (46%), Positives = 226/363 (62%), Gaps = 1/363 (0%) Query: 10 RLIIKVGSSLVTNDGKGLDANAIARWAEQIAQLRALGKEVVLVSSGAVAEGMQRLGFEKR 69 R+++K+G+S++T L + IA + A G EVVLVSSGAV G + LGF R Sbjct: 2 RVVLKLGTSVLTAGTDRLHRPRLVDLMRDIAAVSAQGHEVVLVSSGAVTAGWEALGFPPR 61 Query: 70 PTGIHHLQACAAVGQMGLAQIYETSFRKHDLHTAQVLLTHADLADRERYLNARSTLFALL 129 + Q AAVGQ+ L +Y + + L +AQ+LLT D +R RYLNAR+TL L Sbjct: 62 ERTLAEKQLLAAVGQVQLMHLYTSLAELYGLRSAQLLLTADDFRERTRYLNARTTLEGCL 121 Query: 130 QLGVVPVINENDTVVTDEIKFGDNDTLGALVANLIEAEALIILTDQPGLFTADPRKDPTA 189 GV+PVINENDTV ++IK GDNDTL A VANL+EA+ L+ILTD PGL+TADPR P A Sbjct: 122 SRGVLPVINENDTVAVEQIKVGDNDTLSAFVANLVEADLLLILTDAPGLYTADPRTHPDA 181 Query: 190 TLISEARAGDPSLELIAGGTGTGIGRGGMLTKILAAKRAATSGAHTVIAWGREEQVLTRL 249 TLI P + +AGG G+ G GGM TKI AA+ A +G VIA G + L R+ Sbjct: 182 TLIPVVERVTPDIWALAGGAGSHRGTGGMHTKIQAAEIATRAGTPVVIAPGDLPEALRRV 241 Query: 250 AAGEAIGTQLNAETAQLTARKQWMADHLKTAGRVVLDAGAVQKLSLEGKSLLPIGVVEVS 309 GEA+GT+ A +L ARK+W+ + GR++LD GA Q + G SLLP G+ +V Sbjct: 242 VDGEALGTRFLAHGTRLEARKRWILAEI-AHGRLLLDGGAAQAVRERGSSLLPAGIRQVE 300 Query: 310 GEFGRGDVITCVDENGRAIARGMSNYNSSDARRIIRHPSSEIQAILGFVEEPELVHRDNL 369 G+F RG + + +G+ + RG++ Y + D RR+ H S EI+A+LG+ E VHRD+L Sbjct: 301 GDFERGHTVRLLAPDGQELGRGLTRYRADDLRRVCGHHSREIEALLGYTYGEEAVHRDDL 360 Query: 370 VLL 372 VLL Sbjct: 361 VLL 363 >STM0321 [E] COG0263 Glutamate 5-kinase Sty Salmonella typhimurium LT2 Length = 367 Score = 296 bits (758), Expect = 5e-80, Method: Compositional matrix adjust. Identities = 165/363 (45%), Positives = 226/363 (62%), Gaps = 1/363 (0%) Query: 8 AKRLIIKVGSSLVTNDGKGLDANAIARWAEQIAQLRALGKEVVLVSSGAVAEGMQRLGFE 67 ++ L++K+G+S++T + L+ I Q AQL A G +V+V+SGA+A G + LG+ Sbjct: 4 SQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYP 63 Query: 68 KRPTGIHHLQACAAVGQMGLAQIYETSFRKHDLHTAQVLLTHADLADRERYLNARSTLFA 127 + P I Q AAVGQ L Q++E F + +H Q+LLT AD+ DRER+LNAR TL A Sbjct: 64 ELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDTLRA 123 Query: 128 LLQLGVVPVINENDTVVTDEIKFGDNDTLGALVANLIEAEALIILTDQPGLFTADPRKDP 187 LL +VPVINEND V T EIK GDND L AL A L A+ L++LTDQ GLFTADPR +P Sbjct: 124 LLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQQGLFTADPRSNP 183 Query: 188 TATLISEARAGDPSLELIAGGTGTGIGRGGMLTKILAAKRAATSGAHTVIAWGREEQVLT 247 A LI + D +L IAG + +G+G GGM TK+ AA A +G T+IA G + V+ Sbjct: 184 QAELIKDVYGVDDALRSIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIASGSKPGVIG 243 Query: 248 RLAAGEAIGTQLNAETAQLTARKQWMADHLKTAGRVVLDAGAVQKLSLEGKSLLPIGVVE 307 + G ++GT+ +A+ + L RK+W+ AG + +D GA + G SLLP G+ Sbjct: 244 DVMEGISVGTRFHAQASPLENRKRWIFG-APPAGEITVDEGATAAMLERGSSLLPKGIKS 302 Query: 308 VSGEFGRGDVITCVDENGRAIARGMSNYNSSDARRIIRHPSSEIQAILGFVEEPELVHRD 367 V+G F RG+VI + GR IA G+S YNS RRI H S +I AILG+ P VHRD Sbjct: 303 VTGNFSRGEVIRICNLQGRDIAHGVSRYNSDALRRIAGHHSQQIDAILGYEYGPVAVHRD 362 Query: 368 NLV 370 +++ Sbjct: 363 DMI 365 >proB [E] COG0263 Glutamate 5-kinase Eco Escherichia coli K12 Length = 367 Score = 295 bits (756), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 165/363 (45%), Positives = 224/363 (61%), Gaps = 1/363 (0%) Query: 8 AKRLIIKVGSSLVTNDGKGLDANAIARWAEQIAQLRALGKEVVLVSSGAVAEGMQRLGFE 67 ++ L++K+G+S++T + L+ I Q AQL A G +V+V+SGA+A G + LG+ Sbjct: 4 SQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYP 63 Query: 68 KRPTGIHHLQACAAVGQMGLAQIYETSFRKHDLHTAQVLLTHADLADRERYLNARSTLFA 127 + P I Q AAVGQ L Q++E F + +H Q+LLT AD+ DRER+LNAR TL A Sbjct: 64 ELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRA 123 Query: 128 LLQLGVVPVINENDTVVTDEIKFGDNDTLGALVANLIEAEALIILTDQPGLFTADPRKDP 187 LL +VPVINEND V T EIK GDND L AL A L A+ L++LTDQ GL+TADPR +P Sbjct: 124 LLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRSNP 183 Query: 188 TATLISEARAGDPSLELIAGGTGTGIGRGGMLTKILAAKRAATSGAHTVIAWGREEQVLT 247 A LI + D +L IAG + +G+G GGM TK+ AA A +G T+IA G + V+ Sbjct: 184 QAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIG 243 Query: 248 RLAAGEAIGTQLNAETAQLTARKQWMADHLKTAGRVVLDAGAVQKLSLEGKSLLPIGVVE 307 + G ++GT +A+ L RK+W+ AG + +D GA + G SLLP G+ Sbjct: 244 DVMEGISVGTLFHAQATPLENRKRWIFG-APPAGEITVDEGATAAILERGSSLLPKGIKS 302 Query: 308 VSGEFGRGDVITCVDENGRAIARGMSNYNSSDARRIIRHPSSEIQAILGFVEEPELVHRD 367 V+G F RG+VI + GR IA G+S YNS RRI H S EI AILG+ P VHRD Sbjct: 303 VTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRD 362 Query: 368 NLV 370 +++ Sbjct: 363 DMI 365