BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0329.p (383 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1446 [R] COG3550 Uncharacterized protein related to capsule b... 346 5e-95 hipA [R] COG3550 Uncharacterized protein related to capsule bios... 290 4e-78 ZhipA [R] COG3550 Uncharacterized protein related to capsule bio... 214 2e-55 CC2735 Non COG 110 5e-24 ECs2115 [R] COG3550 Uncharacterized protein related to capsule b... 103 8e-22 >RSc1446 [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Rso Ralstonia solanacearum Length = 445 Score = 346 bits (888), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 189/354 (53%), Positives = 235/354 (66%), Gaps = 7/354 (1%) Query: 1 MNGSFVGTWALTAQG-DSLQYDARWVVSASGRPLSLSLPFRPGNPPHRGDLVRHYFENLL 59 MNG VG W + G L+YD W+ S + RPLSLSLP G P RG +V YF+NLL Sbjct: 10 MNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLL 69 Query: 60 PDSKAIRERLARRYQAADTDAFALLGEAGRDCVGALQILPLGATPELSWTIDARPLRERE 119 P+++ IRER+ RR+ A TDA LL GRDCVGA+Q+LP G P ID+ L + + Sbjct: 70 PEAQPIRERVRRRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEALTDSQ 129 Query: 120 VAELLRQTMEPTPLALTQGE--ELRLSIAGAQEKTALLRYLDQWCLPHGATPTTHILKLP 177 V LLR L G+ R+SIAGAQEKTALLR D W P GATPTTHILKLP Sbjct: 130 VDTLLRNLTAMPALGEDAGDPDAFRISIAGAQEKTALLRIGDAWHRPLGATPTTHILKLP 189 Query: 178 MGLVGGLQLDLRHSVENEWLCSRILAAYGMPVAACDILCFEDQKVLAVERFDRRWSRDPA 237 +GL+G ++ D++ SV+NEWLC+++LAA+G+ VA +I F KVLAVERFDRRW Sbjct: 190 LGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERFDRRW----M 245 Query: 238 SGQAMLLRLPQEDMCQATGISPWLKYEADGGPGMKEIMALLGGAIDPVAARRHFFKAQLL 297 G A + RLPQED CQATG KYE DGGPGM+ + LL + + +R F AQL Sbjct: 246 PGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLA 305 Query: 298 FWMLWATDGHAKNFSLFLKAGGRYEPTPLYDVLSVFPLIGEGPGKLSPHRAAXA 351 FW+L ATDGHAKNFSL + GGR+ TPLYDVLSV+P++G+GP +LSP+RA A Sbjct: 306 FWLLAATDGHAKNFSLAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLA 359 >hipA [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Eco Escherichia coli K12 Length = 440 Score = 290 bits (742), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 155/344 (45%), Positives = 212/344 (61%), Gaps = 17/344 (4%) Query: 1 MNGSFVGTWALTAQG-DSLQYDARWVVSASGRPLSLSLPFRPGNPPHRGDLVRHYFENLL 59 MN VG A G + +Y W+ S RPLSLSLP + GN D V ++F+NLL Sbjct: 8 MNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNIT--SDAVFNFFDNLL 65 Query: 60 PDSKAIRERLARRYQAADTDAFALLGEAGRDCVGALQILPLGAT---PELSWTIDARPLR 116 PDS +R+R+ +RY A F LL E GRD VGA+ ++P T P ++W L Sbjct: 66 PDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAW----EKLT 121 Query: 117 EREVAELLRQTMEPTPLALTQGE-ELRLSIAGAQEKTALLRYLDQWCLPHGATPTTHILK 175 E + E+L PL + + E + R+S+AGAQEKTALLR + WC+P G TPTTHI+K Sbjct: 122 EARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHIIK 181 Query: 176 LPMGLVG--GLQLDLRHSVENEWLCSRILAAYGMPVAACDILCFEDQKVLAVERFDRRWS 233 LP+G + LDL SV+NE+ C + G+ V +I+ + + LAVERFDRRW+ Sbjct: 182 LPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRWN 241 Query: 234 RDPASGQAMLLRLPQEDMCQATGISPWLKYEADGGPGMKEIMALLGGAIDPVAARRHFFK 293 + + +LLRLPQEDMCQ G+ +KYE+DGGPG+ IMA L G+ + + R F K Sbjct: 242 AE----RTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMK 297 Query: 294 AQLLFWMLWATDGHAKNFSLFLKAGGRYEPTPLYDVLSVFPLIG 337 Q+ W++ ATDGHAKNFS+F++AGG Y TP YD++S FP++G Sbjct: 298 FQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLG 341 >ZhipA [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes EcZ Escherichia coli O157:H7 EDL933 Length = 340 Score = 214 bits (546), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 110/237 (46%), Positives = 152/237 (64%), Gaps = 11/237 (4%) Query: 104 PELSWTIDARPLREREVAELLRQTMEPTPLALTQGE-ELRLSIAGAQEKTALLRYLDQWC 162 P ++W L E + E+L PL + + E + R+S+AGAQEKTALLR + WC Sbjct: 13 PIMAW----EKLTEVRLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWC 68 Query: 163 LPHGATPTTHILKLPMGLVG--GLQLDLRHSVENEWLCSRILAAYGMPVAACDILCFEDQ 220 +P G TPTTHI+KLP+G + LDL SV+NE+ C + G+ V +I+ + Sbjct: 69 IPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNV 128 Query: 221 KVLAVERFDRRWSRDPASGQAMLLRLPQEDMCQATGISPWLKYEADGGPGMKEIMALLGG 280 + LAVERFDRRW+ + + +LLRLPQEDMCQ G+ +KYE+DGGPG+ IMA L G Sbjct: 129 RALAVERFDRRWNAE----RTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMG 184 Query: 281 AIDPVAARRHFFKAQLLFWMLWATDGHAKNFSLFLKAGGRYEPTPLYDVLSVFPLIG 337 + + R F K Q+ W++ ATDGHAKNFS+F++AGG Y TP YD++S FP++G Sbjct: 185 FSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLG 241 >CC2735 Non COG Length = 444 Score = 110 bits (276), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 107/328 (32%), Positives = 153/328 (46%), Gaps = 30/328 (9%) Query: 13 AQGDSLQYDARWVVSASGRPLSLSLPFRPGNPPHRGDLV-RHYFENLLPDSKAIRERLAR 71 A+ S YD R++ +A G PLSLS+P + GD+ R +F+NLLP++ + +R+ Sbjct: 26 ARAISFAYDDRYI-AAGGPPLSLSMPLEQVS---FGDVTARAFFDNLLPENDQM-QRVMD 80 Query: 72 RYQAADTDAFALLGEAGRDCVGALQILPLGATPELSWTI---DARPLREREVAELLRQTM 128 R A D LL G DC GA+ LP+GA P + D L +AE+ R Sbjct: 81 REGLARDDIVGLLSHLGADCSGAISCLPIGADPIKVPGVLSEDYELLAPGAIAEIARSLA 140 Query: 129 EPTPLALTQGEELRLSIAGAQEKTALLRYLDQWCLPH--GATPTTHILKLPMGLVGGLQL 186 E L T + +AG Q K AL + P PTTHILK+P + Sbjct: 141 ERQRLPDTITDP--SPVAGVQRKIALTHTPQGFAKPRPGRKVPTTHILKVP-------ET 191 Query: 187 DLRHSVENEWLCSRILAAYGMPVAACDILCFEDQKVLAVERFDRRWSRDPASGQAMLLRL 246 LR E +R+ +A G+ V+ + + + L + RFD R RD ++ RL Sbjct: 192 RLRRDARLEAAAARLASALGLDVSIPEAIVIDGVDALLITRFD-RVVRD-----GVVYRL 245 Query: 247 PQEDMCQATGISPWLKYEADGGPG----MKEIMALLGGAIDPVAARRHFFKAQLLFWMLW 302 QED QA G+ LKY+ +G PG + I +L P +R F A + ++ Sbjct: 246 HQEDFAQAMGLPATLKYQRNGAPGRQFDAQAIARVLDQTEAPALSRTAFLSATIFNLLIG 305 Query: 303 ATDGHAKNFSLFLKAGGRYEPTPLYDVL 330 TD HAKN L + G PLYD+L Sbjct: 306 NTDNHAKNHGLLYRQGRAPILAPLYDLL 333 >ECs2115 [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Ecs Escherichia coli O157:H7 Length = 186 Score = 103 bits (257), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 45/87 (51%), Positives = 61/87 (70%) Query: 251 MCQATGISPWLKYEADGGPGMKEIMALLGGAIDPVAARRHFFKAQLLFWMLWATDGHAKN 310 MCQ G+ +KYE+DGGPG+ IMA L G + + R F K Q+ W++ ATDGHAKN Sbjct: 1 MCQTFGLPSSVKYESDGGPGIARIMAFLMGFSEALRDRYDFMKFQVFQWLIGATDGHAKN 60 Query: 311 FSLFLKAGGRYEPTPLYDVLSVFPLIG 337 FS+F++AGG Y TP YD++S FP++G Sbjct: 61 FSVFIQAGGSYRLTPFYDIISAFPVLG 87