BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0283.p (321 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2233 [R] COG2334 Putative homoserine kinase type II (protein ... 366 e-101 NMB2029 [R] COG2334 Putative homoserine kinase type II (protein ... 241 2e-63 NMA0411 [R] COG2334 Putative homoserine kinase type II (protein ... 239 6e-63 BMEI1458 [R] COG2334 Putative homoserine kinase type II (protein... 219 5e-57 AGc1416 [R] COG2334 Putative homoserine kinase type II (protein ... 217 4e-56 >RSc2233 [R] COG2334 Putative homoserine kinase type II (protein kinase fold) Rso Ralstonia solanacearum Length = 334 Score = 366 bits (939), Expect = e-101, Method: Compositional matrix adjust. Identities = 183/323 (56%), Positives = 227/323 (70%), Gaps = 13/323 (4%) Query: 1 MAVFTPVSLEQLSDWLKHFPLGAATSIKGISSGIENSNFFIGTERG----EYVLTLFEKL 56 MAVFTPV+ +++ WL+ + +G +++GI SGIEN+NFF+ TE+ EYV+TLFE+L Sbjct: 1 MAVFTPVTNAEIALWLEQYDVGTVRALRGIPSGIENTNFFLTTEKDGATHEYVVTLFERL 60 Query: 57 HIEQLPFYLELMRHLAERGIPVPAPVANDKGAIVSLLNDKPAAIVSKLEGQSQLDPQPVH 116 EQLPFYL LM+HLA+ GI VPAP+ GAI+ L KPA IV++L G+S L P Sbjct: 61 TSEQLPFYLYLMQHLAQHGICVPAPIPGRDGAILRPLKGKPATIVTRLPGRSNLAPTTSE 120 Query: 117 CAALGDMLARMHLAAKDFKILQPNLRGLAWWLETTPTVLPFLPAELGRLLADEVQYQKDF 176 CA +GDMLARMHLA +D+ QPNLR L WW E P + PF+ LL E+ +Q+ F Sbjct: 121 CAIVGDMLARMHLAGRDYPRHQPNLRSLPWWNEVVPDIQPFVQGATRELLVAELAHQQRF 180 Query: 177 AATADYRALPRGPVHADLFRNNAMF------VGERLSGIFDFYFAGCDTWLFDLAVTVND 230 +ADY ALP GP H DLFR+N +F ERL G FDFYFAG D WLFD+AVTVND Sbjct: 181 FGSADYAALPEGPCHCDLFRDNVLFEPATDSQPERLGGFFDFYFAGVDKWLFDVAVTVND 240 Query: 231 WCVDLDTGVLDLARTEAMLAAYRAVRPFSEAERIAWQPMLRAAALRFWLSRLYDFYMPRA 290 WCVDL TG LD R AML AY AVRPF++AE W+ MLRAAA RFW+SRL+DF++PR Sbjct: 241 WCVDLATGALDAERMRAMLRAYHAVRPFTDAEARHWRDMLRAAAYRFWVSRLWDFHLPRD 300 Query: 291 TLRAEMLTPHDPTHFERILRLRI 313 AE+L PHDPTHFER+LR R+ Sbjct: 301 ---AELLQPHDPTHFERVLRERV 320 >NMB2029 [R] COG2334 Putative homoserine kinase type II (protein kinase fold) Nme Neisseria meningitidis MC58 Length = 305 Score = 241 bits (615), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 130/309 (42%), Positives = 178/309 (57%), Gaps = 8/309 (2%) Query: 1 MAVFTPVSLEQLSDWLKHFPLGAATSIKGISSGIENSNFFIGTERGEYVLTLFEKLHIEQ 60 M+V+T VS +++ +L + LG S++GI+ GI NSN+F+ T G YVLT+FE L E+ Sbjct: 1 MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTTSGRYVLTVFEVLKQEE 60 Query: 61 LPFYLELMRHLAERGIPVPAPVANDKGAIVSLLNDKPAAIVSKLEGQSQLDPQPVHCAAL 120 LPF+LEL RHL+ +G+ V APVA G + S+L KPA +V+ L+G P C Sbjct: 61 LPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVACLKGSDTALPTAEQCFHT 120 Query: 121 GDMLARMHLAAKDFKILQPNLRGLAWWLETTPTVLPFLPAELGRLLADEVQYQKDFAATA 180 G MLA+MHLAA DF + N R AWW E +LP L + LL E+ KD Sbjct: 121 GAMLAKMHLAAADFPLEMENPRYNAWWTEACARLLPVLSQDDAALLCSEIDALKDNLGN- 179 Query: 181 DYRALPRGPVHADLFRNNAMFVGERLSGIFDFYFAGCDTWLFDLAVTVNDWCVDLDTGVL 240 LP G +HADLF++N + G ++SG DFY+A +++DLA+ VNDW D L Sbjct: 180 ---HLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMYDLAIAVNDWARTADNK-L 235 Query: 241 DLARTEAMLAAYRAVRPFSEAERIAWQPMLRAAALRFWLSRLYDFYMPRATLRAEMLTPH 300 D A +A + Y VRP S E+ + RA +RFW+SRL DF+ P+A EM Sbjct: 236 DEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSRLLDFHFPQA---GEMTFIK 292 Query: 301 DPTHFERIL 309 DP F +L Sbjct: 293 DPNAFRNLL 301 >NMA0411 [R] COG2334 Putative homoserine kinase type II (protein kinase fold) NmA Neisseria meningitidis Z2491 Length = 307 Score = 239 bits (610), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 130/309 (42%), Positives = 178/309 (57%), Gaps = 8/309 (2%) Query: 1 MAVFTPVSLEQLSDWLKHFPLGAATSIKGISSGIENSNFFIGTERGEYVLTLFEKLHIEQ 60 M+V+T VS +++ +L + LG S++GI+ GI NSN+F+ T G YVLT+FE L E+ Sbjct: 3 MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTTSGRYVLTVFEVLKQEE 62 Query: 61 LPFYLELMRHLAERGIPVPAPVANDKGAIVSLLNDKPAAIVSKLEGQSQLDPQPVHCAAL 120 LPF+LEL RHL+ +G+ V APVA G + S+L KPA +V+ L+G P C Sbjct: 63 LPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVACLKGSDTALPTAEQCFHT 122 Query: 121 GDMLARMHLAAKDFKILQPNLRGLAWWLETTPTVLPFLPAELGRLLADEVQYQKDFAATA 180 G MLA+MHLAA DF + N R AWW E +LP L + LL E+ KD Sbjct: 123 GAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQDDAALLRAEIDALKDNLGN- 181 Query: 181 DYRALPRGPVHADLFRNNAMFVGERLSGIFDFYFAGCDTWLFDLAVTVNDWCVDLDTGVL 240 LP G +HADLF++N + G ++SG DFY+A +++DLA+ VNDW D L Sbjct: 182 ---HLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMYDLAIAVNDWARTADNK-L 237 Query: 241 DLARTEAMLAAYRAVRPFSEAERIAWQPMLRAAALRFWLSRLYDFYMPRATLRAEMLTPH 300 D A +A + Y VRP S E+ + RA +RFW+SRL DF+ P+A EM Sbjct: 238 DEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSRLLDFHFPQA---GEMTFIK 294 Query: 301 DPTHFERIL 309 DP F +L Sbjct: 295 DPNAFRNLL 303 >BMEI1458 [R] COG2334 Putative homoserine kinase type II (protein kinase fold) Bme Brucella melitensis Length = 326 Score = 219 bits (559), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 132/320 (41%), Positives = 182/320 (56%), Gaps = 22/320 (6%) Query: 1 MAVFTPVSLEQLSDWLKHFPLGAATSIKGISSGIENSNFFIGTERGEYVLTLFEK-LHIE 59 MAV+T ++ +L +L+H+ +G TS KGI+ G+ENSN+ + T G ++LTL+EK + E Sbjct: 1 MAVYTDINEIELGAFLRHYDIGTLTSYKGIAEGVENSNYLLHTSSGSFILTLYEKRTNRE 60 Query: 60 QLPFYLELMRHLAERGIPVPAPVANDKGAIVSLLNDKPAAIVSKLEGQSQLDPQPVHCAA 119 LPF+L LM+HLA+RG+ P PV + GA++ L +PAAIV+ LEG P HC A Sbjct: 61 DLPFFLGLMQHLAKRGLECPQPVVRNDGAMIGQLAGRPAAIVTFLEGMWMRRPTVAHCEA 120 Query: 120 LGDMLARMHLAAKDFKILQPNLRGLAWWLETTPTVLPFLPAELGRLLAD--------EVQ 171 +G+ LA MHLA DF + + N GL T P P L R AD E + Sbjct: 121 VGEGLAHMHLAGADFPMRRRN--GL-----TLPDWRPLW--NLSRKCADTVERGLVAETE 171 Query: 172 YQKDFAATADYRALPRGPVHADLFRNNAMFVGERLSGIFDFYFAGCDTWLFDLAVTVNDW 231 DF LP+G +HADLF +NA F+G+RLSG DFYFA D +D+AV +N W Sbjct: 172 ADLDFLEKNWPADLPQGVIHADLFPDNAFFLGDRLSGFIDFYFACTDILAYDVAVCLNAW 231 Query: 232 CVDLDTGVLDLARTEAMLAAYRAVRPFSEAERIAWQPMLRAAALRFWLSRLYDFYMPRAT 291 C + D + + A+L Y +VRP SEAE A + R AA+RF L+RLYD+ A Sbjct: 232 CFEKDFSY-NRTKGAALLRGYTSVRPLSEAEANALLVLARGAAVRFMLTRLYDWLTVPA- 289 Query: 292 LRAEMLTPHDPTHFERILRL 311 + DP + R +R Sbjct: 290 --GSFVVKKDPMEYVRRMRF 307 >AGc1416 [R] COG2334 Putative homoserine kinase type II (protein kinase fold) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 322 Score = 217 bits (552), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 118/312 (37%), Positives = 189/312 (60%), Gaps = 6/312 (1%) Query: 1 MAVFTPVSLEQLSDWLKHFPLGAATSIKGISSGIENSNFFIGTERGEYVLTLFEK-LHIE 59 MAV+T ++ ++L ++L + +G+ TS KGI+ G+ENSNF + T + +LTL+EK + Sbjct: 1 MAVYTDITEDELRNFLTQYDVGSLTSYKGIAEGVENSNFLLHTTKDPLILTLYEKRVEKN 60 Query: 60 QLPFYLELMRHLAERGIPVPAPVANDKGAIVSLLNDKPAAIVSKLEGQSQLDPQPVHCAA 119 LPF+L LM+HLA +G+ P P+ G ++ L+ +PAA++S LEG P+ HC Sbjct: 61 DLPFFLGLMQHLAAKGLSCPLPLPRKDGELLGELSGRPAALISFLEGMWLRKPEAKHCRE 120 Query: 120 LGDMLARMHLAAKDFKILQPNLRGLAWWLETTPTVLPFLPAELGRLLADEVQYQKDFAAT 179 +G LA MHLA++ F+I +PN + W + E+ + L +E++ + D+ A Sbjct: 121 VGKALAAMHLASEGFEIKRPNALSVDGW-KVLWDKSEERADEVEKGLREEIRPEIDYLAA 179 Query: 180 ADYRALPRGPVHADLFRNNAMFVGERLSGIFDFYFAGCDTWLFDLAVTVNDWCVDLDTGV 239 + LP G +HADLF++N F+G+ LSG+ DFYFA D +D+++ +N WC + D G Sbjct: 180 HWPKDLPAGVIHADLFQDNVFFLGDELSGLIDFYFACNDLLAYDVSICLNAWCFEKD-GA 238 Query: 240 LDLARTEAMLAAYRAVRPFSEAERIAWQPMLRAAALRFWLSRLYDFYMPRATLRAEMLTP 299 ++ + +A+L Y++VRP SEAE A + R +ALRF+L+RLYD+ A ++ Sbjct: 239 YNVTKGKALLEGYQSVRPLSEAELEALPLLSRGSALRFFLTRLYDWLTTPA---GALVVK 295 Query: 300 HDPTHFERILRL 311 DP + R LR Sbjct: 296 KDPLEYLRKLRF 307