BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0278.p (432 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2230_1 [L] COG0258 5'-3' exonuclease (including N-terminal do... 385 e-106 NMB1982_1 [L] COG0258 5'-3' exonuclease (including N-terminal do... 315 9e-86 NMA0462_1 [L] COG0258 5'-3' exonuclease (including N-terminal do... 314 3e-85 YPO0017_1 [L] COG0258 5'-3' exonuclease (including N-terminal do... 253 5e-67 PM1065_1 [L] COG0258 5'-3' exonuclease (including N-terminal dom... 249 6e-66 >RSc2230_1 [L] COG0258 5'-3' exonuclease (including N-terminal domain of PolI) Rso Ralstonia solanacearum Length = 295 Score = 385 bits (988), Expect = e-106, Method: Compositional matrix adjust. Identities = 187/262 (71%), Positives = 214/262 (81%), Gaps = 1/262 (0%) Query: 1 MRNAEGAPTGALYGIINMLRRLRNDYPASYVACVFDAKGKTFRDDLYPDYKATRKSMPED 60 +RN EG PTGA+YGI+NMLR+LRNDYPA Y ACVFDAKGKTFRD+LYP YK R MP+D Sbjct: 29 LRNGEGFPTGAIYGIVNMLRKLRNDYPAKYSACVFDAKGKTFRDELYPAYKEHRAPMPDD 88 Query: 61 LALQIEPIHAAVRALGWPILMVEGIEADDVIGTLSVQATAAGLDTIVSTGDKDMAQLVNE 120 L QIEPIH AVRALGWPILMV+G+EADDVIGTL+ +A G+ T+VSTGDKD+AQLVN+ Sbjct: 89 LRQQIEPIHEAVRALGWPILMVDGVEADDVIGTLAERAAREGIRTVVSTGDKDLAQLVND 148 Query: 121 HVTLVNTMSNEVLDIAGVTAKFGVPPERIVDYLSLIGDTVDNVPGVEKCGPKTAVKWLTA 180 HVTLVNTMSNE LD AGV AKFGVPP +IVDYLSLIGDTVDNVPGV K GPKTAVKWL Sbjct: 149 HVTLVNTMSNETLDPAGVLAKFGVPPGKIVDYLSLIGDTVDNVPGVPKVGPKTAVKWLGE 208 Query: 181 YGSLDGVMANADKISGVVGENLRRALDWLPQGRVLVTVKTDCDLSAHMSSILESLTMQPQ 240 YGSLD V+A A +I GVVGENLR LDWLP+GR L+TVKTDCDL+ + + E+L + Sbjct: 209 YGSLDSVIARAGEIKGVVGENLRNTLDWLPKGRELLTVKTDCDLATEVPT-FEALVDGGE 267 Query: 241 DKDVLKELFTRYNFRTWLRELS 262 D+ L + F RY F+TWLRE S Sbjct: 268 DRAKLVDFFGRYGFKTWLREAS 289 >NMB1982_1 [L] COG0258 5'-3' exonuclease (including N-terminal domain of PolI) Nme Neisseria meningitidis MC58 Length = 300 Score = 315 bits (808), Expect = 9e-86, Method: Compositional matrix adjust. Identities = 161/266 (60%), Positives = 192/266 (72%) Query: 5 EGAPTGALYGIINMLRRLRNDYPASYVACVFDAKGKTFRDDLYPDYKATRKSMPEDLALQ 64 +GAPTGALYG++NMLRRLR++YP Y A VFDAKGK FR ++ +YKATR MP+DL Q Sbjct: 31 DGAPTGALYGVLNMLRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQ 90 Query: 65 IEPIHAAVRALGWPILMVEGIEADDVIGTLSVQATAAGLDTIVSTGDKDMAQLVNEHVTL 124 E + VR GWP+L++ +EADDVIGTL+ Q GL IVSTGDKDMAQLV+E VTL Sbjct: 91 AEALPDLVRLTGWPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTL 150 Query: 125 VNTMSNEVLDIAGVTAKFGVPPERIVDYLSLIGDTVDNVPGVEKCGPKTAVKWLTAYGSL 184 VNTMS+E LDI GV AKFGV P++I DYL+L+GD VDNVPGVEKCGPKTAVKWL AYGSL Sbjct: 151 VNTMSSETLDIEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSL 210 Query: 185 DGVMANADKISGVVGENLRRALDWLPQGRVLVTVKTDCDLSAHMSSILESLTMQPQDKDV 244 GVM +A +I G VGENL+ AL LP LVT+KTD DL A +S +ESL Sbjct: 211 AGVMEHASEIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQ 270 Query: 245 LKELFTRYNFRTWLRELSDGAEAGAS 270 L F R+ FRTWL+E G++ Sbjct: 271 LVVDFKRWGFRTWLKEAESNMNTGST 296 >NMA0462_1 [L] COG0258 5'-3' exonuclease (including N-terminal domain of PolI) NmA Neisseria meningitidis Z2491 Length = 300 Score = 314 bits (804), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 160/266 (60%), Positives = 192/266 (72%) Query: 5 EGAPTGALYGIINMLRRLRNDYPASYVACVFDAKGKTFRDDLYPDYKATRKSMPEDLALQ 64 +GAPTGALYG++NMLRRLR++YP Y A VFDAKGK FR ++ +YKATR MP+DL Q Sbjct: 31 DGAPTGALYGVLNMLRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQ 90 Query: 65 IEPIHAAVRALGWPILMVEGIEADDVIGTLSVQATAAGLDTIVSTGDKDMAQLVNEHVTL 124 E + VR GWP+L++ +EADDVIGTL+ Q GL IVSTGDKDMAQLV+E VTL Sbjct: 91 AEALPDLVRLTGWPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTL 150 Query: 125 VNTMSNEVLDIAGVTAKFGVPPERIVDYLSLIGDTVDNVPGVEKCGPKTAVKWLTAYGSL 184 VNTMS+E LDI GV AKFGV P++I DYL+L+GD VDNVPGVEKCGPKTAVKWL AYGSL Sbjct: 151 VNTMSSETLDIEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSL 210 Query: 185 DGVMANADKISGVVGENLRRALDWLPQGRVLVTVKTDCDLSAHMSSILESLTMQPQDKDV 244 GV+ +A +I G VGENL+ AL LP LVT+KTD DL A +S +ESL Sbjct: 211 QGVIEHASEIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRCTTPKWAQ 270 Query: 245 LKELFTRYNFRTWLRELSDGAEAGAS 270 L F R+ FRTWL+E G++ Sbjct: 271 LVVDFKRWGFRTWLKEAESNMNTGST 296 >YPO0017_1 [L] COG0258 5'-3' exonuclease (including N-terminal domain of PolI) Ype Yersinia pestis Length = 287 Score = 253 bits (647), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 128/264 (48%), Positives = 173/264 (65%), Gaps = 11/264 (4%) Query: 1 MRNAEGAPTGALYGIINMLRRLRNDYPASYVACVFDAKGKTFRDDLYPDYKATRKSMPED 60 + N G PTGA+YG++NMLR L Y S+VA VFDAKGKTFRD+L+ +YK+ R MP+D Sbjct: 28 LTNGSGEPTGAMYGVLNMLRSLLLQYRPSHVAVVFDAKGKTFRDELFAEYKSHRPPMPDD 87 Query: 61 LALQIEPIHAAVRALGWPILMVEGIEADDVIGTLSVQATAAGLDTIVSTGDKDMAQLVNE 120 L +QIEP+H V+A+G P+L+V G+EADDVIGTL+ +A AG ++STGDKDMAQLV Sbjct: 88 LRVQIEPLHQMVKAMGLPLLVVSGVEADDVIGTLAREAEKAGHSVLISTGDKDMAQLVTP 147 Query: 121 HVTLVNTMSNEVLDIAGVTAKFGVPPERIVDYLSLIGDTVDNVPGVEKCGPKTAVKWLTA 180 ++TL+NTM+N +L V K+GVPPE I+D+L+L+GD+ DN+PGV G KTA L Sbjct: 148 NITLINTMNNTILGPQDVCDKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQG 207 Query: 181 YGSLDGVMANADKISGVVGENLRRALDWLPQGR-------VLVTVKTDCDLSAHMSSILE 233 G LD + +N DKIS + + L Q + L T+KTD +L + Sbjct: 208 LGGLDTLFSNLDKISTLTFRGAKTMSAKLEQNKDVAYLSYKLATIKTDVELDV----TCD 263 Query: 234 SLTMQPQDKDVLKELFTRYNFRTW 257 LT+ P D L +LF+RY F+ W Sbjct: 264 ELTVSPPDDKQLHQLFSRYEFKRW 287 >PM1065_1 [L] COG0258 5'-3' exonuclease (including N-terminal domain of PolI) Pmu Pasteurella multocida Length = 282 Score = 249 bits (637), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 126/259 (48%), Positives = 173/259 (66%), Gaps = 11/259 (4%) Query: 1 MRNAEGAPTGALYGIINMLRRLRNDYPASYVACVFDAKGKTFRDDLYPDYKATRKSMPED 60 + N+ PTGA+YG++NML+ L + S++A VFDAKGKTFRD+++ YK+ R MPE+ Sbjct: 28 LTNSLNEPTGAMYGVLNMLKSLISQVQPSHIAVVFDAKGKTFRDEIFEQYKSHRPPMPEE 87 Query: 61 LALQIEPIHAAVRALGWPILMVEGIEADDVIGTLSVQATAAGLDTIVSTGDKDMAQLVNE 120 L QI P+H +RALG P+L+VEG+EADDVIGTL+VQA+ AG ++STGDKDMAQLV+E Sbjct: 88 LRSQILPLHNIIRALGIPLLVVEGVEADDVIGTLAVQASRAGKKVLISTGDKDMAQLVDE 147 Query: 121 HVTLVNTMSNEVLDIAGVTAKFGVPPERIVDYLSLIGDTVDNVPGVEKCGPKTAVKWLTA 180 ++ L+NTM+N +LD GV K+G+PPE I+DYL+L+GD+ DN+PG+ G KTA+ L Sbjct: 148 NIMLINTMTNTLLDREGVLEKYGLPPELIIDYLALMGDSSDNIPGIPGVGEKTALGLLQG 207 Query: 181 YGSLDGVMANADKISGV-------VGENLRRALDWLPQGRVLVTVKTDCDLSAHMSSILE 233 GS+ + AN DK++ + +G L A D +L T+KTD L E Sbjct: 208 IGSMAEIYANLDKVASLSLRGAKTLGAKLAEAKDLADLSYLLATIKTDVALDVSP----E 263 Query: 234 SLTMQPQDKDVLKELFTRY 252 LT +KD L E F RY Sbjct: 264 QLTFGVANKDALIEYFARY 282