BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0275.p (294 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2228 [Q] COG0412 Dienelactone hydrolase and related enzymes R... 359 4e-99 alr1362 [Q] COG0412 Dienelactone hydrolase and related enzymes N... 323 3e-88 Z5352 [Q] COG0412 Dienelactone hydrolase and related enzymes EcZ... 295 9e-80 ECs4760 [Q] COG0412 Dienelactone hydrolase and related enzymes E... 295 9e-80 YPO3787 [Q] COG0412 Dienelactone hydrolase and related enzymes Y... 294 2e-79 >RSc2228 [Q] COG0412 Dienelactone hydrolase and related enzymes Rso Ralstonia solanacearum Length = 293 Score = 359 bits (922), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 179/298 (60%), Positives = 218/298 (73%), Gaps = 9/298 (3%) Query: 1 MNDLRNDFNSLLGQSTLSGNADRRSFLKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEV 60 M++L + SL+ + DRRSF+KTALG+ FAAAVLPV AQ I TD GLT GEV Sbjct: 1 MHELDAEVESLVPGRSF----DRRSFVKTALGSAFAAAVLPVSAQA-IHTDFNGLTVGEV 55 Query: 61 SIEVNGQRVPAYRAQPEGKTGLPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFAR 120 ++ V +VPAYRAQPEGKTGLPV+IVVSEIFGVHE+IAD+ RRFAKLGYLAIAPD F R Sbjct: 56 TVPVGDFKVPAYRAQPEGKTGLPVMIVVSEIFGVHEYIADVCRRFAKLGYLAIAPDLFVR 115 Query: 121 QGNPMAYTSIAELQKDIISKVPDAQVMGDIDAYVKWAGEHGGDTRRLGINGFCWGGRIVW 180 QG+P +Y +I ELQ ++ISKVPDAQV+GD+DA V WA ++GGD R+GI GFCWGGR W Sbjct: 116 QGDPQSYGTIQELQANVISKVPDAQVIGDLDATVAWARQNGGDPARIGITGFCWGGRQAW 175 Query: 181 LYSAHNPKVKAGAAWYGRLVGEKSELFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQ 240 L++ H+P +KA WYG L G + L P PID LK PV+GLYG KD GI V+ Sbjct: 176 LFAEHSPDIKAAVVWYGHLKGNATALQPAFPIDKVADLKAPVLGLYGAKDTGITQDAVQA 235 Query: 241 MKEALQKAGN----KSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA 294 MK AL+ + N S VY ++GHAF+ADYRPSYV+ADA+D W K +A+FK HGVA Sbjct: 236 MKAALEASANPRAKASRIIVYPDAGHAFHADYRPSYVKADAEDGWKKCLAWFKDHGVA 293 >alr1362 [Q] COG0412 Dienelactone hydrolase and related enzymes Nos Nostoc sp. PCC 7120 Length = 285 Score = 323 bits (828), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 161/281 (57%), Positives = 203/281 (72%), Gaps = 11/281 (3%) Query: 23 RRSFLKTA-LGTGFAAAVLPVCAQTMIKTDTQGLTAGEVSIEVNGQRVPAYRAQPEGKTG 81 RR F+ TA L TGFA AV P+ AQ +I TDTQGL AG V I V +PAYRA P Sbjct: 6 RRQFIATATLTTGFALAVKPISAQ-VITTDTQGLVAGAVKIPVQDGEIPAYRALPATGEN 64 Query: 82 LPVIIVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFARQGNPMAYTSIAELQKDIISKV 141 P+++V+ EIFGVHEHI D+ RRFAKLGYLAIAP+ FARQG+ ++I E+++ +++KV Sbjct: 65 FPIVLVIQEIFGVHEHIQDVCRRFAKLGYLAIAPELFARQGDVSKLSNIDEIRQ-VVAKV 123 Query: 142 PDAQVMGDIDAYVKWAGEHG-GDTRRLGINGFCWGGRIVWLYSAHNPKVKAGAAWYGRLV 200 PDAQV D+DA V WA + G+ +L I GFCWGGRI WLYSA+NPKVKAG AWYGRLV Sbjct: 124 PDAQVFSDLDATVGWAVKSSKGNADQLAITGFCWGGRITWLYSAYNPKVKAGVAWYGRLV 183 Query: 201 GEKSELFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQM-------KEALQKAGNKSE 253 G +EL P+ P+DIAP L PV+GLYGGKD GIP+ TVEQM + L+ + +KSE Sbjct: 184 GNSTELTPKHPVDIAPNLTVPVLGLYGGKDTGIPLDTVEQMRVGIARRRHRLKSSSSKSE 243 Query: 254 FHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQHGVA 294 VY ++ HAF ADYRPSY E +A+D W ++ A+FKQ+GV+ Sbjct: 244 IVVYPDAPHAFFADYRPSYREKEAQDGWKRLQAWFKQYGVS 284 >Z5352 [Q] COG0412 Dienelactone hydrolase and related enzymes EcZ Escherichia coli O157:H7 EDL933 Length = 293 Score = 295 bits (755), Expect = 9e-80, Method: Compositional matrix adjust. Identities = 140/267 (52%), Positives = 184/267 (68%), Gaps = 2/267 (0%) Query: 27 LKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEVSIEVNGQRVPAYRAQPEGKTG-LPVI 85 + T +GFA A P+ A T+++T + AG SI G +PAY A+P+ G LPV+ Sbjct: 23 MATTQQSGFAPAASPL-ASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVV 81 Query: 86 IVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFARQGNPMAYTSIAELQKDIISKVPDAQ 145 IVV EIFGVHEHI DI RR A GYLAIAP+ + R+G+P + I L +++KVPD+Q Sbjct: 82 IVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQ 141 Query: 146 VMGDIDAYVKWAGEHGGDTRRLGINGFCWGGRIVWLYSAHNPKVKAGAAWYGRLVGEKSE 205 V+ D+D WA +GGD RL I GFCWGGRI WLY+AHNP++KA AWYG+L G+KS Sbjct: 142 VLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGKLTGDKSL 201 Query: 206 LFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQMKEALQKAGNKSEFHVYMNSGHAFN 265 P+QP+DIA L PV+GLYGG D IP +VE M++AL+ A K+E VY ++GHAFN Sbjct: 202 NSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFN 261 Query: 266 ADYRPSYVEADAKDAWSKVVAFFKQHG 292 ADYRPSY A A+D W +++ +FKQ+G Sbjct: 262 ADYRPSYHAASAEDGWQRMLEWFKQYG 288 >ECs4760 [Q] COG0412 Dienelactone hydrolase and related enzymes Ecs Escherichia coli O157:H7 Length = 293 Score = 295 bits (755), Expect = 9e-80, Method: Compositional matrix adjust. Identities = 140/267 (52%), Positives = 184/267 (68%), Gaps = 2/267 (0%) Query: 27 LKTALGTGFAAAVLPVCAQTMIKTDTQGLTAGEVSIEVNGQRVPAYRAQPEGKTG-LPVI 85 + T +GFA A P+ A T+++T + AG SI G +PAY A+P+ G LPV+ Sbjct: 23 MATTQQSGFAPAASPL-ASTIVQTPDDAIVAGFTSIPSQGDNMPAYHARPKQSDGPLPVV 81 Query: 86 IVVSEIFGVHEHIADIARRFAKLGYLAIAPDFFARQGNPMAYTSIAELQKDIISKVPDAQ 145 IVV EIFGVHEHI DI RR A GYLAIAP+ + R+G+P + I L +++KVPD+Q Sbjct: 82 IVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFADIPTLLSGLVAKVPDSQ 141 Query: 146 VMGDIDAYVKWAGEHGGDTRRLGINGFCWGGRIVWLYSAHNPKVKAGAAWYGRLVGEKSE 205 V+ D+D WA +GGD RL I GFCWGGRI WLY+AHNP++KA AWYG+L G+KS Sbjct: 142 VLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHNPQLKAAVAWYGKLTGDKSL 201 Query: 206 LFPQQPIDIAPTLKTPVIGLYGGKDQGIPVSTVEQMKEALQKAGNKSEFHVYMNSGHAFN 265 P+QP+DIA L PV+GLYGG D IP +VE M++AL+ A K+E VY ++GHAFN Sbjct: 202 NSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQALRAANAKAEIIVYPDAGHAFN 261 Query: 266 ADYRPSYVEADAKDAWSKVVAFFKQHG 292 ADYRPSY A A+D W +++ +FKQ+G Sbjct: 262 ADYRPSYHAASAEDGWQRMLEWFKQYG 288 >YPO3787 [Q] COG0412 Dienelactone hydrolase and related enzymes Ype Yersinia pestis Length = 267 Score = 294 bits (752), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 134/244 (54%), Positives = 173/244 (70%), Gaps = 1/244 (0%) Query: 52 TQGLTAGEVSIEVNGQRVPAYRAQPEGKTG-LPVIIVVSEIFGVHEHIADIARRFAKLGY 110 T G+ GE +I G +PAY A+P+ TG PV+IVV EIFGVHEHI DI RR AK GY Sbjct: 15 THGIHCGETTIPSQGDELPAYIAKPDQHTGPYPVVIVVQEIFGVHEHIQDICRRLAKQGY 74 Query: 111 LAIAPDFFARQGNPMAYTSIAELQKDIISKVPDAQVMGDIDAYVKWAGEHGGDTRRLGIN 170 LAIAP+ + RQG+ Y++I EL +++ KVPD QV+ D+D WA HGGDT++L I Sbjct: 75 LAIAPELYFRQGDAKDYSNINELVNNLVKKVPDRQVLVDLDHTAHWASRHGGDTKKLAIT 134 Query: 171 GFCWGGRIVWLYSAHNPKVKAGAAWYGRLVGEKSELFPQQPIDIAPTLKTPVIGLYGGKD 230 GFCWGGRI WLY+AHNP++KA AWYG+LVGEK+ P+ P+D+A L PV+GLYGGKD Sbjct: 135 GFCWGGRIAWLYAAHNPQLKAAVAWYGKLVGEKTLFLPKYPVDVAIDLCAPVLGLYGGKD 194 Query: 231 QGIPVSTVEQMKEALQKAGNKSEFHVYMNSGHAFNADYRPSYVEADAKDAWSKVVAFFKQ 290 IP +E M++AL+ A +E VY +GHAFNADYRPSY A+D W +++ +F Q Sbjct: 195 TSIPAEHIETMRQALRAANADAEIIVYPEAGHAFNADYRPSYHAESAQDGWQRMLDWFTQ 254 Query: 291 HGVA 294 HGV+ Sbjct: 255 HGVS 258