BLASTP 2.2.21 [Jun-14-2009]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0008.0271.p         (166 letters)

Database: COGmodified 
           192,987 sequences; 59,019,183 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

PA0147 [R] COG3491 Isopenicillin N synthase and related dioxygen...   168   4e-42
CC0200 Non COG                                                        109   2e-24
PA4191 [R] COG3491 Isopenicillin N synthase and related dioxygen...   103   1e-22
SPAC25B8.13c [R] COG3491 Isopenicillin N synthase and related di...    98   9e-21
mll0111 [R] COG3491 Isopenicillin N synthase and related dioxyge...    93   3e-19

>PA0147 [R] COG3491 Isopenicillin N synthase and related dioxygenases Pae
           Pseudomonas aeruginosa
          Length = 320

 Score =  168 bits (426), Expect = 4e-42,   Method: Compositional matrix adjust.
 Identities = 76/159 (47%), Positives = 104/159 (65%)

Query: 2   IALSLEMPEDFFTPVLHTPIATQRMLHYPPQPASAQHNQIGAGAHTDWGLVTILAQDAIG 61
           +A++L + EDFF      P++  R++HYPP  A    +Q GAGAHTD+G VT+L QDA G
Sbjct: 154 LAIALGIEEDFFDRRFEQPVSVFRLIHYPPASARQSADQPGAGAHTDYGCVTLLYQDAAG 213

Query: 62  GLEICNAEGDWVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVCNVGNAPRYSLPFFQD 121
           GL++ N +G+W+ APPI G+FVVNIGD++ RWSND Y S  HRV +     RYS+PFF +
Sbjct: 214 GLQVQNRQGEWIDAPPIDGTFVVNIGDMMARWSNDRYRSTPHRVISPRGVHRYSMPFFAE 273

Query: 122 GDQAAVVACLPTCCSAERPARYAPCTIGDYLEMKVKQTF 160
                 + CLP C  A+ P +Y P T GD+L  +  QT+
Sbjct: 274 PHMDTEIRCLPGCFDADNPPKYPPTTCGDWLTSRFAQTY 312


>CC0200 Non COG
          Length = 311

 Score =  109 bits (273), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 59/162 (36%), Positives = 85/162 (52%), Gaps = 7/162 (4%)

Query: 2   IALSLEMPEDFFTPVLHTPIATQRMLHYPPQPASAQHNQIGAGAHTDWGLVTILAQDAIG 61
           IA  L++  DFF P +    +  R+LHYPP P  A    + AGAH D   +T+L     G
Sbjct: 148 IATYLKLERDFFKPTVQDGNSVLRLLHYPPIPKDA--TGVRAGAHGDINTITLLLGAEEG 205

Query: 62  GLEICNAEGDWVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVCN----VGNAPRYSLP 117
           GLE+ + +G W+   P  G  V+NIGD+L+R +N++  S  HRV N        PRYS P
Sbjct: 206 GLEVLDRDGQWLPINPPPGCLVINIGDMLERLTNNVLPSTVHRVVNPPPERRGVPRYSTP 265

Query: 118 FFQDGDQAAVVACLPTCCSAERPARYAPCTIGD-YLEMKVKQ 158
           FF        +  L  C +AE P RY      D +L+ ++++
Sbjct: 266 FFLHFASDYEIKTLQNCVTAENPDRYPESITADEFLQQRLRE 307


>PA4191 [R] COG3491 Isopenicillin N synthase and related dioxygenases Pae
           Pseudomonas aeruginosa
          Length = 334

 Score =  103 bits (258), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 69/173 (39%), Positives = 89/173 (51%), Gaps = 15/173 (8%)

Query: 2   IALSLEMPEDFFTP-VLHTPIATQRMLHYPPQPASAQHN-QIGAGAHTDWGLVTILAQDA 59
           IAL L +  D+F       P+   R+ +YP QP     + Q G G HTD+GL+T+L QDA
Sbjct: 155 IALGLGLEADYFAARYTGDPLILFRLFNYPSQPVPEGLDVQWGVGEHTDYGLLTLLHQDA 214

Query: 60  IGGLEICNAEGDWVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVC-NVGNAPRYSLPF 118
           IGGL++   +G W+ APPI GSFV N+GD+L+R +  LY S  HRV  N     R S P 
Sbjct: 215 IGGLQVRTPQG-WLEAPPIPGSFVCNLGDMLERMTGGLYRSTPHRVARNTSGRDRLSFPL 273

Query: 119 FQDGDQAAVV---ACLPTCCSAERPARYAP--------CTIGDYLEMKVKQTF 160
           F D +  A V     LP     +  AR              GDYL  KV + F
Sbjct: 274 FFDPNFHARVQPIEGLPEVPEQDDSARRWDQANVHAFHGEYGDYLLNKVAKVF 326


>SPAC25B8.13c [R] COG3491 Isopenicillin N synthase and related dioxygenases Spo
           Schizosaccharomyces pombe
          Length = 397

 Score = 97.8 bits (242), Expect = 9e-21,   Method: Compositional matrix adjust.
 Identities = 53/161 (32%), Positives = 86/161 (53%), Gaps = 10/161 (6%)

Query: 1   VIALSLEMPEDFFTPVLHTPIATQRMLHYPPQPASAQHNQIGAGAHTDWGLVTILAQDAI 60
           ++A SLE+  D F      P  + R+L YP  P     N++G   HTD   +T+++QD +
Sbjct: 236 LLAESLELSPDAFDEFCSDPTTSIRLLRYPSSP-----NRLGVQEHTDADALTLMSQDNV 290

Query: 61  GGLEICNAEGD-WVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVCNVGNAPRYSLPFF 119
            GLEI +   + ++S  P  G+ + N+GD++   +N+ Y S+ HRVCN   + RY++PFF
Sbjct: 291 KGLEILDPVSNCFLSVSPAPGALIANLGDIMAILTNNRYKSSMHRVCNNSGSDRYTIPFF 350

Query: 120 QDGDQAAVVACLPTCCSAERPARYAPCTIGDYLEMKVKQTF 160
             G+   VVA LP       P+   P  + D L    + ++
Sbjct: 351 LQGNIDYVVAPLPGLG----PSTAEPIAVEDLLRDHFQNSY 387


>mll0111 [R] COG3491 Isopenicillin N synthase and related dioxygenases Mlo
           Mesorhizobium loti
          Length = 342

 Score = 92.8 bits (229), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 57/166 (34%), Positives = 79/166 (47%), Gaps = 20/166 (12%)

Query: 2   IALSLEMPEDFFTPVLHTPIATQRMLHYPPQPASA---------------QHNQIGAGAH 46
           IA  L++PE  F       I+T R++ YP + A+A               +   I    H
Sbjct: 157 IARGLDLPETIFDSYFEHGISTLRLIRYPLRDANAGIDISGPEFSVVHKGETRTIIGREH 216

Query: 47  TDWGLVTILAQDAIGGLEICNAEGDWVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVC 106
            D G VT+LAQD + GL+  N  G+W+  PP   +  VN G LL+RW+     +  HRV 
Sbjct: 217 ADSGFVTLLAQDGVEGLQAKNLAGEWIDVPPANSTLAVNFGQLLERWTGGRVRATRHRVI 276

Query: 107 NVGNAPRYSLPFFQDGDQAAVVACLPTCCSAERPARYAPCTIGDYL 152
               A R+S+PFF +    A +A LP   S   P  + P   GDYL
Sbjct: 277 APKTA-RFSIPFFYEPRVDAEIAPLP--LSGAEP--FEPFLYGDYL 317