BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0185.p (282 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3975 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidi... 158 2e-38 thiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine... 96 6e-20 ZthiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidin... 96 6e-20 ECs2906 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 96 6e-20 lin0667 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimid... 96 1e-19 >PA3975 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Pae Pseudomonas aeruginosa Length = 265 Score = 158 bits (399), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 93/263 (35%), Positives = 146/263 (55%), Gaps = 10/263 (3%) Query: 4 QTP---PLILSFGAADPVGATGVQADLATYAAMGCHGLSVITSLLIGDTARVEDVQVVEV 60 +TP P++L DP G G+QAD+ A GCH +T+L + DT V D +V++ Sbjct: 2 KTPTSRPVVLCLSGHDPSGGAGLQADIEALLAQGCHAAPAVTALTVQDTVNVSDFRVLDR 61 Query: 61 DWVADQARVILEDMAVAAFKVGNVGSIENISVIAEIVSDYPDVPLILDP-FTSALANQED 119 +WV QA +L D+ VAA K+G +GS+E + +AEI++ +P +P++ DP + Sbjct: 62 EWVLAQANAVLADLPVAAVKLGMLGSLEMVDTVAEILAAHPHLPVVCDPVLRAGGGGALG 121 Query: 120 EDDRLIAIRELLIPQATLMVASAGELARLAETWREPVPGDALMLDAMRIIEMGCEYLLVT 179 + D A+RE L+P+AT+ + E LA+ +P + A R++E CE+LL+T Sbjct: 122 KGDVGFAMRERLLPRATIATPNLPEARLLAD-----LPEGSADQCAERLLEH-CEHLLIT 175 Query: 180 NTQTDLQEIANSLFDESGLVRQDAWQRLPGSFVGAGTTLSAAMAAMLANGLEVPLAAAEA 239 D E+ N L+ G R QRLPGS+ G+G TL++A+A +A G + A A Sbjct: 176 GGHGDESEVHNRLYSRDGSRRTFTCQRLPGSYHGSGCTLASALAGRIALGEALHGAVQSA 235 Query: 240 QEFTLAALANAQRIGMGKLVPDR 262 ++T L +A+ G G+ VP R Sbjct: 236 LDYTWRTLRDAESPGHGQYVPRR 258 >thiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Eco Escherichia coli K12 Length = 266 Score = 96.3 bits (238), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 81/267 (30%), Positives = 131/267 (49%), Gaps = 19/267 (7%) Query: 10 LSFGAADPVGATGVQADLATYAAMGCHGLSVITSLLIGDTARVEDVQVVEVDWVADQARV 69 L+ DP G G+QADL T++A+G +G SVIT+L+ +T V+ V +E D+VA Q Sbjct: 7 LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS 66 Query: 70 ILEDMAVAAFKVGNVGSIENISVIAEIVSDYPDVPLILDPFTSALANQED---EDDRLIA 126 + D+ + K+G + + + +AE + Y ++LD T LA D + Sbjct: 67 VFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLD--TVMLAKSGDPLLSPSAVAT 124 Query: 127 IRELLIPQATLMVASAGELARL-----AETWREPVPGDALMLDAMR-IIEMGCEYLLVTN 180 +R L+PQ +L+ + E A L A T +E ML+ R ++ MGC +L+ Sbjct: 125 LRSRLLPQVSLITPNLPEAAALLDAPHARTEQE-------MLEQGRSLLAMGCGAVLMKG 177 Query: 181 TQTDLQEIANSLFDESGLVRQDAWQRLPGSFVGAGTTLSAAMAAMLANGLEVPLAAAEAQ 240 D ++ + LF G R A + + + G G TLSAA+AA+ EA+ Sbjct: 178 GHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAK 237 Query: 241 EFTLAALANAQRIGMGKLV-PDRYFWA 266 + +ALA A + +G + P +F A Sbjct: 238 SWLSSALAQADTLEVGHGIGPVHHFHA 264 >ZthiD [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase EcZ Escherichia coli O157:H7 EDL933 Length = 266 Score = 96.3 bits (238), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 81/267 (30%), Positives = 131/267 (49%), Gaps = 19/267 (7%) Query: 10 LSFGAADPVGATGVQADLATYAAMGCHGLSVITSLLIGDTARVEDVQVVEVDWVADQARV 69 L+ DP G G+QADL T++A+G +G SVIT+L+ +T V+ V +E D+VA Q Sbjct: 7 LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS 66 Query: 70 ILEDMAVAAFKVGNVGSIENISVIAEIVSDYPDVPLILDPFTSALANQED---EDDRLIA 126 + D+ + K+G + + + +AE + Y ++LD T LA D + Sbjct: 67 VFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLD--TVMLAKSGDPLLSPSAVAT 124 Query: 127 IRELLIPQATLMVASAGELARL-----AETWREPVPGDALMLDAMR-IIEMGCEYLLVTN 180 +R L+PQ +L+ + E A L A T +E ML+ R ++ MGC +L+ Sbjct: 125 LRSRLLPQVSLITPNLPEAAALLDAPHARTEQE-------MLEQGRALLAMGCGAVLMKG 177 Query: 181 TQTDLQEIANSLFDESGLVRQDAWQRLPGSFVGAGTTLSAAMAAMLANGLEVPLAAAEAQ 240 D ++ + LF G R A + + + G G TLSAA+AA+ EA+ Sbjct: 178 GHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAK 237 Query: 241 EFTLAALANAQRIGMGKLV-PDRYFWA 266 + +ALA A + +G + P +F A Sbjct: 238 SWLSSALAQADTLEVGHGIGPVHHFHA 264 >ECs2906 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Ecs Escherichia coli O157:H7 Length = 266 Score = 96.3 bits (238), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 81/267 (30%), Positives = 131/267 (49%), Gaps = 19/267 (7%) Query: 10 LSFGAADPVGATGVQADLATYAAMGCHGLSVITSLLIGDTARVEDVQVVEVDWVADQARV 69 L+ DP G G+QADL T++A+G +G SVIT+L+ +T V+ V +E D+VA Q Sbjct: 7 LTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFVAAQLDS 66 Query: 70 ILEDMAVAAFKVGNVGSIENISVIAEIVSDYPDVPLILDPFTSALANQED---EDDRLIA 126 + D+ + K+G + + + +AE + Y ++LD T LA D + Sbjct: 67 VFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLD--TVMLAKSGDPLLSPSAVAT 124 Query: 127 IRELLIPQATLMVASAGELARL-----AETWREPVPGDALMLDAMR-IIEMGCEYLLVTN 180 +R L+PQ +L+ + E A L A T +E ML+ R ++ MGC +L+ Sbjct: 125 LRSRLLPQVSLITPNLPEAAALLDAPHARTEQE-------MLEQGRALLAMGCGAVLMKG 177 Query: 181 TQTDLQEIANSLFDESGLVRQDAWQRLPGSFVGAGTTLSAAMAAMLANGLEVPLAAAEAQ 240 D ++ + LF G R A + + + G G TLSAA+AA+ EA+ Sbjct: 178 GHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAK 237 Query: 241 EFTLAALANAQRIGMGKLV-PDRYFWA 266 + +ALA A + +G + P +F A Sbjct: 238 SWLSSALAQADTLEVGHGIGPVHHFHA 264 >lin0667 [H] COG0351 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase Lin Listeria innocua Length = 271 Score = 95.5 bits (236), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 75/247 (30%), Positives = 123/247 (49%), Gaps = 15/247 (6%) Query: 10 LSFGAADPVGATGVQADLATYAAMGCHGLSVITSLLIGDTAR--VEDVQVVEVDWVADQA 67 L+ +D G G+QADL T+ G +G S IT+++ D V ++ V +Q Sbjct: 7 LTIAGSDSSGGAGLQADLKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQL 66 Query: 68 RVILEDMAVAAFKVGNVGSIENISVIAEIVSDYPDVPLILDPFTSALANQEDE---DDRL 124 + IL V A K G +GSI+ I E + Y +++DP + EDE + Sbjct: 67 KTILSGGPVDAMKTGMLGSIDIIKATREAIDKYDLKNVVIDPVM--VCKGEDELIQPENA 124 Query: 125 IAIRELLIPQATLMVAS---AGELARLAETWREPVPGDALMLDAMRIIEMGCEYLLVTNT 181 AIR+LL+P+AT+ + AG+L+ L + D + A +IIE+G +Y+++ Sbjct: 125 EAIRDLLLPKATITTPNLFEAGQLSGLGKL----TTLDDMKKAAKKIIELGAKYVVIKGG 180 Query: 182 QT-DLQEIANSLFDESGLVRQDAWQRLPGSFVGAGTTLSAAMAAMLANGLEVPLAAAEAQ 240 + + + + L+D + + P GAG T +AA+ A LA GL V A A+A+ Sbjct: 181 KALESDKAIDLLYDGKEFTVYEVEKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAK 240 Query: 241 EFTLAAL 247 +F AA+ Sbjct: 241 DFVTAAI 247