BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0064.p (296 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Cgl1134 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 263 4e-70 AGc4592 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 261 1e-69 Cgl2996 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 256 6e-68 Cgl2351 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 135 8e-32 PA2507 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit ... 123 5e-28 >Cgl1134 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 295 Score = 263 bits (672), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 139/283 (49%), Positives = 182/283 (64%), Gaps = 4/283 (1%) Query: 14 EDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICS 73 E+ + + VL+ + RL+ +M SL HLH F R+V+LTE+EW IDFLT+ G I Sbjct: 11 EEDLVERVLASFDSCENPRLKLVMKSLTVHLHDFIRDVRLTEEEWNYAIDFLTKVGHITD 70 Query: 74 DQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQYPLGADIANGAKGE 133 D+RQEF+LLSDTLG S +A N TEATVFGPF V+DAP G DIA GA G+ Sbjct: 71 DKRQEFVLLSDTLGASMQTIAVNNEAYEDATEATVFGPFFVDDAPLVQNGDDIAFGAVGQ 130 Query: 134 PCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGRYHFRS 193 P +V G+V+ G P+ +AR+EVW+ DE+G YDVQYAD ++ R L++D G YHF Sbjct: 131 PAWVEGTVKDTEGNPIPNARIEVWECDEDGLYDVQYAD-ERSAGRAHLYSDENGEYHFWG 189 Query: 194 ILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLD-SDA 252 + PYPIPHDGPVG+ML+ +GR P R AHLHF + A TL+TH+F D L+ D+ Sbjct: 190 LTPVPYPIPHDGPVGQMLQAVGRSPVRCAHLHFMVTAPEKRTLVTHIFVEGDPQLEIGDS 249 Query: 253 VFGVRSTLIADWPCHPAGTA-PDGTQC-EQPFYTLDYDFVLNP 293 VFGV+ +LI + PAGTA PDG +Q + +D VL P Sbjct: 250 VFGVKDSLIKKFVEQPAGTATPDGRDVGDQTWARTRFDIVLAP 292 >AGc4592 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 295 Score = 261 bits (668), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 132/286 (46%), Positives = 184/286 (64%), Gaps = 14/286 (4%) Query: 14 EDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICS 73 E+ + V++R D RL+EIM + + LH +E++ TE+EW I FLTE GQIC+ Sbjct: 14 EERSAETVIARMRDCDDPRLKEIMAVVTRKLHEAVKEIEPTEEEWMKAIHFLTEVGQICN 73 Query: 74 DQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQYPLGADIANGAKGE 133 + RQE+IL SD LG+S LV A + KP+G +E+TV GPFHV DAP+ P+GA+I KGE Sbjct: 74 EWRQEWILFSDILGVSMLVDAINHRKPSGASESTVLGPFHVADAPEMPMGANICLDGKGE 133 Query: 134 PCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLD-QYRARGTLFTDAEGRYHFR 192 V+G + G P+A AR++VWQA++ GFYDVQ + + RG T +GRY FR Sbjct: 134 DMVVTGRILDTEGAPVAGARIDVWQANDEGFYDVQQKGIQPDFNLRGVFVTGEDGRYWFR 193 Query: 193 SILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSDA 252 + YPIP DGPVGR+L +GRHP+RPAHLH+ + AE + TL+TH+F DD Y+ SDA Sbjct: 194 AAKPKYYPIPDDGPVGRLLRAMGRHPYRPAHLHYIVSAESFTTLVTHIFDPDDPYIRSDA 253 Query: 253 VFGVRSTLIADW-------PCHPAGTAPDGTQCEQPFYTLDYDFVL 291 VFGV+ +L+A++ G A D F+++++DFVL Sbjct: 254 VFGVKESLLANFDRVEDREKAQELGFAGDW------FWSVNHDFVL 293 >Cgl2996 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 301 Score = 256 bits (653), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 135/283 (47%), Positives = 176/283 (62%), Gaps = 4/283 (1%) Query: 14 EDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICS 73 E+ + VL + RL+++M SLV HLH F R+V+LTE EW IDFLT G I Sbjct: 18 EENLVNRVLQSFDACENPRLKQLMESLVVHLHDFIRDVRLTEDEWNYAIDFLTAVGHITD 77 Query: 74 DQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQYPLGADIANGAKGE 133 D+RQEF+LLSDTLG S +A N TEATVFGPF ++DAP+ LG DIA GA+G+ Sbjct: 78 DKRQEFVLLSDTLGASMQTIAVNNEAYENSTEATVFGPFFLDDAPEVELGGDIAGGAQGQ 137 Query: 134 PCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGRYHFRS 193 ++ G+V G P+ +AR+EVW+ DE+G YDVQYAD ++ R + TDA G Y F Sbjct: 138 AAWIEGTVTDTEGNPVPNARIEVWECDEDGLYDVQYAD-ERMAGRAYMHTDANGDYRFWG 196 Query: 194 ILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLD-SDA 252 + PYPIPHDGPVG ML+ +GR P R AHLHF + A TL+TH+F D L+ D+ Sbjct: 197 LTPVPYPIPHDGPVGNMLKAVGRSPVRCAHLHFMVTAPELRTLVTHIFVEGDPQLEIGDS 256 Query: 253 VFGVRSTLIADWPCHPAGT-APDGTQC-EQPFYTLDYDFVLNP 293 VFGV+ +LI + GT PDG +Q + +D VL P Sbjct: 257 VFGVKDSLIKKFEEQAPGTPTPDGRDLGDQTWARTRFDIVLAP 299 >Cgl2351 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 285 Score = 135 bits (341), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 77/257 (29%), Positives = 125/257 (48%), Gaps = 13/257 (5%) Query: 29 TDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSDQRQEFILLSDTLGL 88 + +R I L+ + A + ++T +E+ ++ + G+ E+ L D Sbjct: 33 SKERANAIYVDLLAAIAQVAHKHEVTYEEYAVLKQWMIDVGEYG-----EWPLWLDVFVE 87 Query: 89 STLVMAQQNHKPAGCTEATVFGPFHVEDAPQYPLGADIANGAKGEPC---FVSGSVRGRN 145 + N T+ ++ GP++VE++P+ P A++ K C G V + Sbjct: 88 HEIEEINYNRHDYTGTKGSIEGPYYVENSPKLPWDAEMPMRDKDRACTPLIFEGQVTDLD 147 Query: 146 GRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGRYHFRSILANPYPIPHDG 205 G L A +E+W ADE+G+Y + ++ RGT+ TD EGRY +++ PY IPHDG Sbjct: 148 GNGLDGAEVELWHADEDGYYSQFAPGIPEWNLRGTIVTDEEGRYKIKTLQPAPYQIPHDG 207 Query: 206 PVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSDAVFGVRSTLIADWP 265 P G +E G HPWRPAHLH R+ GY T+ T ++ ++++D V+ L+ Sbjct: 208 PTGWFIESYGGHPWRPAHLHLRVSHPGYRTITTQLYFEGGEWVENDVATAVKPELV---- 263 Query: 266 CHPAGTAPDGTQCEQPF 282 HP T DG PF Sbjct: 264 LHPE-TGEDGNHVHYPF 279 >PA2507 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Pae Pseudomonas aeruginosa Length = 310 Score = 123 bits (309), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 92/271 (33%), Positives = 134/271 (49%), Gaps = 14/271 (5%) Query: 30 DQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSDQRQEFILLSDTLG-- 87 D R + ++ ++ E+++T E++ +D+L G RQE LL+ LG Sbjct: 27 DPRTKALVRRILDDTAKLIEEMQVTPDEFWKAVDYLNRLGS-----RQEAGLLAAGLGLE 81 Query: 88 --LSTLVMAQQNHKP-AGCTEATVFGPFHVEDAPQYPLGADIANGAK-GEPCFVSGSVRG 143 L L+ AQ G T T+ GP +V AP A + +G G F+ G V G Sbjct: 82 HYLDLLLDAQDAEAGLTGGTPRTIEGPLYVAGAPLSDGEARMDDGRDAGTVMFLQGRVSG 141 Query: 144 RNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGRYHFRSILANPYPIPH 203 +G+PLA A ++VW A+ G Y + +Y R + TDA+GRY RSI+ + Y P Sbjct: 142 PDGQPLAGAIVDVWHANTQGTYSYFDSSQSEYNLRRRIRTDADGRYRARSIVPSGYGCPS 201 Query: 204 DGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSDAVFGVRSTLIAD 263 DGP +L++LGRH RPAH+HF + A G+ L T + D YL D + R LI D Sbjct: 202 DGPTQELLDRLGRHGQRPAHIHFFVSAPGHRHLTTQINLAGDRYLWDDFAYATRDGLIGD 261 Query: 264 --WPCHPAGTAPDGTQCEQPFYTLDYDFVLN 292 + PA G + + F LD+DF L Sbjct: 262 LRFNDDPAAARDRGVEGGR-FAELDFDFQLQ 291