BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0063.p (356 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc4594 [C] COG1454 Alcohol dehydrogenase, class IV Atu Agrobact... 330 3e-90 Cgl2997 [C] COG1454 Alcohol dehydrogenase, class IV Cgl Coryneba... 301 2e-81 Cgl1133 [C] COG1454 Alcohol dehydrogenase, class IV Cgl Coryneba... 301 2e-81 YGL256w [C] COG1454 Alcohol dehydrogenase, class IV Sce Saccharo... 128 2e-29 fucO [C] COG1454 Alcohol dehydrogenase, class IV Eco Escherichia... 125 1e-28 >AGc4594 [C] COG1454 Alcohol dehydrogenase, class IV Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 351 Score = 330 bits (846), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 187/352 (53%), Positives = 235/352 (66%), Gaps = 1/352 (0%) Query: 1 MQAFTYTSAAARVIFGHGSLQHLVRETELLGAQRALVLCTPGQRAEAERVAALIGARCAG 60 MQ F Y +A AR++F GS + E LG RALVL TP Q+ +AE +A+ +G AG Sbjct: 1 MQPFVYMAAPARIVFSAGSSADVAEEIRRLGLSRALVLSTPQQKGDAEALASRLGRLAAG 60 Query: 61 IFDQAVMHVPIETARHAREVARALGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYA 120 +F +A MH P+E + A E RA GADC +++GGGST GLGKAIAL ++ + IPTTYA Sbjct: 61 VFSEAAMHTPVEVTKTAVEAYRAAGADCVVSLGGGSTTGLGKAIALRTDAAQIVIPTTYA 120 Query: 121 GSEMTPIYGLTEAGRKKTGRDARVLPRTVIYDPALSLGLPLDLSVTSGINAMAHAAEGLY 180 GSE+TPI G TE G K T R +LP VIYD L+LGLP+ +S+TSG+NAMAHAAE LY Sbjct: 121 GSEVTPILGQTENGVKTTMRGPEILPEVVIYDAELTLGLPVAISMTSGLNAMAHAAEALY 180 Query: 181 APDANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWLCGTVLGNVGMSLHHK 240 A D NPI S++A EGLRA+ E LP + DI AR LYGAWLCGTVLG VGMSLHHK Sbjct: 181 ARDRNPIASMMAVEGLRAMIEALPVVRQAPHDIGARETALYGAWLCGTVLGAVGMSLHHK 240 Query: 241 LCHTLGGSYNLPHAQTHTVILPHALAYNAAAAPQAMQRIARALGVEDAAQGVYALARALH 300 LCHTLGGS +LPHA+TH V+LPH +AY AAP + +A +G A G++ A L Sbjct: 241 LCHTLGGSLDLPHAETHAVLLPHTIAYVEEAAPNLLAPLAALVGGR-AGAGLFDFAARLG 299 Query: 301 APLSLKELGMPYEALAQCTEIALQNAYANPRPLQREALHALLEDAWHGRPPA 352 AP SL LG+ + L E+A N Y PRP+++ A+ LL+ A+ G PA Sbjct: 300 APSSLAALGVGADDLDPMAELATANPYWCPRPIEKTAIRDLLQRAFEGARPA 351 >Cgl2997 [C] COG1454 Alcohol dehydrogenase, class IV Cgl Corynebacterium glutamicum Length = 363 Score = 301 bits (770), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 163/352 (46%), Positives = 229/352 (65%), Gaps = 4/352 (1%) Query: 3 AFTYTSAAARVIFGHG-SLQHLVRETELLGAQRALVLCTPGQRAEAERVAALIGARCAGI 61 AF + + +V+FG+G S L +E E G+ + +V+ + + A +VA+ I A Sbjct: 6 AFNHDTLPQKVMFGYGKSSAFLKQEVERRGSAKVMVIAGEREMSIAHKVASEI--EVAIW 63 Query: 62 FDQAVMHVPIETARHAREVARALGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG 121 D+ VMHVPIE A AR VA D + +GGGST+GL KAIA+ + LPI+AIPTTYAG Sbjct: 64 HDEVVMHVPIEVAERARAVATDNEIDLLVCVGGGSTIGLAKAIAMTTALPIVAIPTTYAG 123 Query: 122 SEMTPIYGLTEAGRKKTGRDARVLPRTVIYDPALSLGLPLDLSVTSGINAMAHAAEGLYA 181 SE T ++GLTEA RK TG D +VLP TVIYD L++ LP+++SV SG+N +AH + L+ Sbjct: 124 SEATNVWGLTEAARKTTGVDLKVLPETVIYDSELTMSLPVEMSVASGLNGLAHCIDSLWG 183 Query: 182 PDANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWLCGTVLGNVGMSLHHKL 241 P+A+PI ++LA EG+RAL +GLP++ A I+ R LYGA+L + G LHHK+ Sbjct: 184 PNADPINAVLAAEGIRALNQGLPKIVANPHSIEGRDEALYGAYLAAVSFASAGSGLHHKI 243 Query: 242 CHTLGGSYNLPHAQTHTVILPHALAYNAAAAPQAMQRIARALGVEDAAQGVYALARALHA 301 CHTLGG++NLPHAQTH +LP+ LA+NA AP+A +R A A G + A +G+ L +++A Sbjct: 244 CHTLGGTFNLPHAQTHATVLPYVLAFNAGDAPEAERRAAAAFGTDTALEGLQRLRLSVNA 303 Query: 302 PLSLKELGMPYEALAQCTEIALQNAYA-NPRPLQREALHALLEDAWHGRPPA 352 P L + G +A+ ++ L+ A NPRP+ RE L LLE A +G PA Sbjct: 304 PKRLSDYGFEASGIAEAVDVTLEKVPANNPRPVTRENLSRLLEAALNGEDPA 355 >Cgl1133 [C] COG1454 Alcohol dehydrogenase, class IV Cgl Corynebacterium glutamicum Length = 356 Score = 301 bits (770), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 164/351 (46%), Positives = 219/351 (62%), Gaps = 4/351 (1%) Query: 4 FTYTSAAARVIFGHG-SLQHLVRETELLGAQRALVLCTPGQRAEAERVAALIGARCAGIF 62 F + + RV+FG G + Q+L E L A+ +V+ + A +VAA I + Sbjct: 5 FDHETLGQRVLFGSGEAAQNLAAEISRLDAKNVMVVAGDFELPMARQVAADIDVKV--WH 62 Query: 63 DQAVMHVPIETARHAREVARALGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAGS 122 VMHVPIETA AR VA+ D + +GGGST GL KAIA+ + LPI+A+PTTYAGS Sbjct: 63 SNVVMHVPIETAEEARSVAKENDIDVVVCVGGGSTTGLAKAIAMTTALPIIAVPTTYAGS 122 Query: 123 EMTPIYGLTEAGRKKTGRDARVLPRTVIYDPALSLGLPLDLSVTSGINAMAHAAEGLYAP 182 E T ++GLTEA RK TG D +VLP TVIYD AL++ LP+++SV SG+N +AH + L+ P Sbjct: 123 EATNVWGLTEAARKTTGVDNKVLPVTVIYDSALTMSLPVEMSVASGLNGLAHCIDSLWGP 182 Query: 183 DANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWLCGTVLGNVGMSLHHKLC 242 A+PI + +A EG+RAL+ GLP++ A A D+D R LYGA+L + G LHHK+C Sbjct: 183 KADPINAAMAAEGIRALSAGLPKIVADAQDVDGRDEALYGAYLAAVSFASAGSGLHHKIC 242 Query: 243 HTLGGSYNLPHAQTHTVILPHALAYNAAAAPQAMQRIARALGVEDAAQGVYALARALHAP 302 H LGG++NLPHAQTH +LP+ LA+NA APQA QR A A G A +G+ L + AP Sbjct: 243 HVLGGTFNLPHAQTHATVLPYVLAFNAPYAPQAEQRAAAAFGSATALEGLQQLRAQVGAP 302 Query: 303 LSLKELGMPYEALAQCTEIALQNAYA-NPRPLQREALHALLEDAWHGRPPA 352 L + G + + EI L+ A NPR + E L ALL A +G PA Sbjct: 303 QRLSDYGFTAAGIPEAVEIILEKVPANNPRTVTEENLTALLTTALNGDDPA 353 >YGL256w [C] COG1454 Alcohol dehydrogenase, class IV Sce Saccharomyces cerevisiae Length = 465 Score = 128 bits (321), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 105/368 (28%), Positives = 163/368 (44%), Gaps = 36/368 (9%) Query: 14 IFGHGSLQHLVRETELLGAQRALVLCTPGQRAE--AERVAALIGAR--CAGIFDQAVMHV 69 FG G+L+ + ++AL++ PG A + RV ++ R I+D+ + Sbjct: 97 FFGEGALEETADYIKNKDYKKALIVTDPGIAAIGLSGRVQKMLEERDLNVAIYDKTQPNP 156 Query: 70 PIETARHAREVARALGADCAIAIGGGSTMGLGKAIAL------------------ESELP 111 I +V + ++ ++IGGGS KAIAL ++ LP Sbjct: 157 NIANVTAGLKVLKEQNSEIVVSIGGGSAHDNAKAIALLATNGGEIGDYEGVNQSKKAALP 216 Query: 112 ILAIPTTY-AGSEMT--PIYGLTEAGRKKTGRDARVLPRTVIYDPALSLGLPLDLSVTSG 168 + AI TT SEMT I E K D V P + DP+ GLP L+ +G Sbjct: 217 LFAINTTAGTASEMTRFTIISNEEKKIKMAIIDNNVTPAVAVNDPSTMFGLPPALTAATG 276 Query: 169 INAMAHAAEGLYAPDANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWLCGT 228 ++A+ H E + +NPI A +G+ + E L D AR+ Y +L G Sbjct: 277 LDALTHCIEAYVSTASNPITDACALKGIDLINESLVAAYKDGKDKKARTDMCYAEYLAGM 336 Query: 229 VLGNVGMSLHHKLCHTLGGSYNLPHAQTHTVILPHALAYNAAAAPQAMQR---IARALGV 285 N + H L H LGG Y+LPH + V+LPH N P+A +R IA G Sbjct: 337 AFNNASLGYVHALAHQLGGFYHLPHGVCNAVLLPHVQEAN-MQCPKAKKRLGEIALHFGA 395 Query: 286 -----EDAAQGVYALARALHAPLSLKELGMPYEALAQCTEIALQNA--YANPRPLQREAL 338 E+ + ++ L R ++ P +LKELG+ E E A+ +A NP +E + Sbjct: 396 SQEDPEETIKALHVLNRTMNIPRNLKELGVKTEDFEILAEHAMHDACHLTNPVQFTKEQV 455 Query: 339 HALLEDAW 346 A+++ A+ Sbjct: 456 VAIIKKAY 463 >fucO [C] COG1454 Alcohol dehydrogenase, class IV Eco Escherichia coli K12 Length = 383 Score = 125 bits (314), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 112/374 (29%), Positives = 165/374 (44%), Gaps = 46/374 (12%) Query: 15 FGHGSLQHLVRETELLGAQRALVLCTPGQRAEAERVAALIGARCAG-----IFDQAVMHV 69 FG G++ L E + G Q+AL++ T + VA + A I+D V + Sbjct: 14 FGRGAVGALTDEVKRRGYQKALIV-TDKTLVQCGVVAKVTDKMDAAGLAWAIYDGVVPNP 72 Query: 70 PIETARHAREVARALGADCAIAIGGGSTMGLGKAIALESE-------------------- 109 I + V + GAD IAIGGGS KAI + S Sbjct: 73 TITVVKEGLGVFQNSGADYLIAIGGGSPQDTCKAIGIISNNPEFADVRSLEGLSPTNKPS 132 Query: 110 LPILAIPTTYAGS--EMTPIYGLT--EAGRKKTGRDARVLPRTVIYDPALSLGLPLDLSV 165 +PILAIPTT AG+ E+T Y +T E RK D +P+ D + G+P L Sbjct: 133 VPILAIPTT-AGTAAEVTINYVITDEEKRRKFVCVDPHDIPQVAFIDADMMDGMPPALKA 191 Query: 166 TSGINAMAHAAEGLYAPDANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWL 225 +G++A+ HA EG A + L + + +A L + A D DA G ++ Sbjct: 192 ATGVDALTHAIEGYITRGAWALTDALHIKAIEIIAGAL--RGSVAGDKDAGEEMALGQYV 249 Query: 226 CGTVLGNVGMSLHHKLCHTLGGSYNLPHAQTHTVILPHALAYNAAAAPQAMQRIARALGV 285 G NVG+ L H + H LG YN PH + ++LPH + YNA + + IAR +GV Sbjct: 250 AGMGFSNVGLGLVHGMAHPLGAFYNTPHGVANAILLPHVMRYNADFTGEKYRDIARVMGV 309 Query: 286 E-----------DAAQGVYALARALHAPLSLKELGMPYEALAQCTEIALQNAYA--NPRP 332 + A + V+AL R + P L+++G+ E + + AL + NPR Sbjct: 310 KVEGMSLEEARNAAVEAVFALNRDVGIPPHLRDVGVRKEDIPALAQAALDDVCTGGNPRE 369 Query: 333 LQREALHALLEDAW 346 E + L AW Sbjct: 370 ATLEDIVELYHTAW 383