BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0008.0049.p (527 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0365 [E] COG2986 Histidine ammonia-lyase Rso Ralstonia solana... 592 e-169 BH1982 [E] COG2986 Histidine ammonia-lyase Bha Bacillus halodurans 270 9e-72 BS_hutH [E] COG2986 Histidine ammonia-lyase Bsu Bacillus subtilis 268 3e-71 SA0008 [E] COG2986 Histidine ammonia-lyase Sau Staphylococcus au... 249 1e-65 AGl1825 [E] COG2986 Histidine ammonia-lyase Atu Agrobacterium tu... 246 9e-65 >RSp0365 [E] COG2986 Histidine ammonia-lyase Rso Ralstonia solanacearum Length = 528 Score = 592 bits (1525), Expect = e-169, Method: Compositional matrix adjust. Identities = 312/503 (62%), Positives = 366/503 (72%), Gaps = 10/503 (1%) Query: 13 APIRFGAQPLAIEDVVALARRERSAQLHDAPDFRARIARGADFLDRLLREEGTIYGVTTG 72 APIRFG L+IE VVA+ARR+ + L+ +R RI RGA FL L + T+YGV TG Sbjct: 17 APIRFGDCHLSIEAVVAIARRQATVALNGDEAWRDRIERGARFLQEQLAQGATVYGVNTG 76 Query: 73 YGDSCTVTVPPELVPELPHHLYTYHGCGLGALFTPEQARAILAARLNSLCQGFSGVSVAL 132 YGD+C ++VPP LV LP L YHGCG+G P + A++AARLNSL G+SGV L Sbjct: 77 YGDACEISVPPALVQALPLQLTRYHGCGMGRYLAPSETLAVIAARLNSLAHGYSGVRYTL 136 Query: 133 LEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLAAVLCGERDVWREGATVPAAQALAAA 192 L+++ L+ HD+LP+IP EGSVGASGDLTPLSY+AA L GERDV +G A+ A Sbjct: 137 LQRLADLINHDILPRIPAEGSVGASGDLTPLSYVAAALVGERDVLFDGKVRAASDVWRAL 196 Query: 193 GMTPLRLRPKEGLAIMNGTAVMTALACLAFDRARYLCQLATRITAMSSFTLDGNAHHFDA 252 G +P L PKEGLA+MNGTAVMT LACLA+ RA +L +LATR+TA+++ LDG + HFDA Sbjct: 197 GHSPFELAPKEGLALMNGTAVMTGLACLAYARAEHLIRLATRLTALATVALDGRSGHFDA 256 Query: 253 TLFSAKPHPGQQQVAAWLQRDLPCGQAHRNEKRLQDRYSVRCAPHVIGVLNDALPSLRQF 312 T+F AKPH GQ VAAWL+ DL G RLQDRYS+RCAPHVIGV DA+ +R+ Sbjct: 257 TIFQAKPHAGQADVAAWLREDL-AGWDDTGGHRLQDRYSIRCAPHVIGVAQDAMSWIRRD 315 Query: 313 IENELNSANDNPLIDPDGERVLHGGHFYGGHIAFAMDSMKTAVANIADLLDRQMALMVDQ 372 IENELNSANDNPLIDPD R+LHGG+FYGGHIAFAMD++KTA+AN+ADL+DRQ+AL+VD Sbjct: 316 IENELNSANDNPLIDPDARRILHGGNFYGGHIAFAMDALKTAIANLADLMDRQLALLVDD 375 Query: 373 RYNNGLPANLSGAQGARXQPINHGLKALQISASAWTAEALKLTMPASVFSRSTECHNQDK 432 RY+NGLP LSGA R PINHG KA+QIS SAWTAEALK TMPASVFSRSTE HNQDK Sbjct: 376 RYSNGLPRGLSGASPDRA-PINHGFKAIQISVSAWTAEALKHTMPASVFSRSTESHNQDK 434 Query: 433 VSMGTIAARDCLRVLELTEQVAAALLITVRQGVWLRSQRQQVGDTPLQDLPAGR-LAAMQ 491 VSMGTIAARDCLRVLELTEQVAAA + Q V LR+ D L PA R Sbjct: 435 VSMGTIAARDCLRVLELTEQVAAAHTLATAQAVRLRAGL----DAALAIPPAVRAFVERV 490 Query: 492 AALSEDIAVVAEDRMLEPDLRLL 514 ALSE V EDR LE DLR L Sbjct: 491 TALSE---AVTEDRPLEMDLRRL 510 >BH1982 [E] COG2986 Histidine ammonia-lyase Bha Bacillus halodurans Length = 511 Score = 270 bits (689), Expect = 9e-72, Method: Compositional matrix adjust. Identities = 162/480 (33%), Positives = 266/480 (55%), Gaps = 18/480 (3%) Query: 48 RIARGADFLDRLLREEGTIYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGALFTP 107 ++ + +++++ +E IYG+TTG+G + + P+ V L H+L H CG+G+ F Sbjct: 36 KVKQSRKAVEQIVADEKIIYGITTGFGKFSDIFIDPDDVENLQHNLIYSHACGVGSPFPE 95 Query: 108 EQARAILAARLNSLCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLA 167 +R +L R N+L +GFSGV ++E++ L+ ++ P IP +GS+GASGDL PLS+LA Sbjct: 96 TVSRTMLVLRANALLKGFSGVRPLVIERLLALVNANIHPVIPQQGSLGASGDLAPLSHLA 155 Query: 168 AVLCGERDVWREGATVPAAQALAAAGMTPLRLRPKEGLAIMNGTAVMTALACLAFDRARY 227 VL GE +V+ +G A+ AL + P+ L KEGLA++NGT MTA+ +A+ A Sbjct: 156 LVLLGEGEVFYKGTKTKASFALKEEEIEPITLTAKEGLALINGTQAMTAMGVIAYLEAEK 215 Query: 228 LCQLATRITAMSSFTLDGNAHHFDATLFSAKPHPGQQQVAAWLQRDLPCGQ--AHRNEKR 285 L + I +++ L G FD + A+ + Q VA ++ L Q + E R Sbjct: 216 LAFQSEIIASLTMEGLRGIIDAFDEQIHFARGYVEQVDVARRMESYLQDSQLTTRQGELR 275 Query: 286 LQDRYSVRCAPHVIGVLNDALPSLRQFIENELNSANDNPLIDPDGERVLHGGHFYGGHIA 345 +QD YS+RC P V G L +++ +E E+N+A DNPLI +G++V+ GG+F+G IA Sbjct: 276 VQDAYSLRCIPQVHGATWQTLRYVKEKLEIEMNAATDNPLIFDNGQKVISGGNFHGQQIA 335 Query: 346 FAMDSMKTAVANIADLLDRQMALMVDQRYNNGLPANLSGAQGARXQPINHGLKALQISAS 405 AMD + A+A +A++ +R++ +V+ + N+ LP LS A G + G+ LQ A+ Sbjct: 336 LAMDFLGIAMAELANISERRIERLVNPQLND-LPPFLSAAPG-----VQSGVMILQYCAA 389 Query: 406 AWTAEALKLTMPASVFSRSTECHNQDKVSMGTIAARDCLRVLELTEQVAAALLITVRQGV 465 + +E L PASV S + + +D VSMGTI +R ++++ V A LI Q V Sbjct: 390 SLVSENKTLAHPASVDSIPSSANQEDHVSMGTIGSRHAYQIIQNVRNVLAIELICAMQAV 449 Query: 466 WLRSQRQQVGDTPLQDLPAGRLAAMQAALSEDIAVVAEDRMLEPDLRLLLQRIRAQAWSL 525 +R R+++ + L GR E + + +DRM D+ + ++ +W Sbjct: 450 DIRG-REKMASFTKKILEKGR---------EHVPYIDQDRMFAKDIERAAKWLKDGSWDF 499 >BS_hutH [E] COG2986 Histidine ammonia-lyase Bsu Bacillus subtilis Length = 508 Score = 268 bits (684), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 161/423 (38%), Positives = 241/423 (56%), Gaps = 8/423 (1%) Query: 48 RIARGADFLDRLLREEGTIYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGALFTP 107 R+ + ++R++R+E TIYG+ TG+G V + E L +L H CG+G F Sbjct: 33 RVKKSRAAVERIVRDEKTIYGINTGFGKFSDVLIQKEDSAALQLNLILSHACGVGDPFPE 92 Query: 108 EQARAILAARLNSLCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLA 167 +RA+L R N+L +GFSGV L+EQ+ L + P IP +GS+GASGDL PLS+LA Sbjct: 93 CVSRAMLLLRANALLKGFSGVRAELIEQLLAFLNKRVHPVIPQQGSLGASGDLAPLSHLA 152 Query: 168 AVLCGERDVWREGATVPAAQALAAAGMTPLRLRPKEGLAIMNGTAVMTALACLAFDRARY 227 L G+ +V+ EG +PA L AG+ P+ L KEGLA++NGT MTA+ +A+ A Sbjct: 153 LALIGQGEVFFEGERMPAMTGLKKAGIQPVTLTSKEGLALINGTQAMTAMGVVAYIEAEK 212 Query: 228 LCQLATRITAMSSFTLDGNAHHFDATLFSAKPHPGQQQVAAWLQRDLPCG--QAHRNEKR 285 L RI +++ L G FD + A+ + Q VA ++ L + E R Sbjct: 213 LAYQTERIASLTIEGLQGIIDAFDEDIHLARGYQEQIDVAERIRFYLSDSGLTTSQGELR 272 Query: 286 LQDRYSVRCAPHVIGVLNDALPSLRQFIENELNSANDNPLIDPDGERVLHGGHFYGGHIA 345 +QD YS+RC P V G L +++ +E E+N+A DNPLI DG++V+ GG+F+G IA Sbjct: 273 VQDAYSLRCIPQVHGATWQTLGYVKEKLEIEMNAATDNPLIFNDGDKVISGGNFHGQPIA 332 Query: 346 FAMDSMKTAVANIADLLDRQMALMVDQRYNNGLPANLSGAQGARXQPINHGLKALQISAS 405 FAMD +K A++ +A++ +R++ +V+ + N+ LP LS G + G +Q +A+ Sbjct: 333 FAMDFLKIAISELANIAERRIERLVNPQLND-LPPFLSPHPG-----LQSGAMIMQYAAA 386 Query: 406 AWTAEALKLTMPASVFSRSTECHNQDKVSMGTIAARDCLRVLELTEQVAAALLITVRQGV 465 + +E L PASV S + + +D VSMGTIAAR +V+ T +V A I Q V Sbjct: 387 SLVSENKTLAHPASVDSIPSSANQEDHVSMGTIAARHAYQVIANTRRVIAIEAICALQAV 446 Query: 466 WLR 468 R Sbjct: 447 EYR 449 >SA0008 [E] COG2986 Histidine ammonia-lyase Sau Staphylococcus aureus N315 Length = 511 Score = 249 bits (635), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 156/457 (34%), Positives = 247/457 (54%), Gaps = 17/457 (3%) Query: 19 AQPLAIEDVVALARRERSAQLHDAPDFRARIARGADFLDRLLREEGTIYGVTTGYGDSCT 78 + L IED+ + +++ ++ D D R+ + ++R++ E T+YG+TTG+G Sbjct: 14 GETLTIEDIKSFLQQQSKIEIID--DALERVKKSRAVVERIIENEETVYGITTGFGLFSD 71 Query: 79 VTVPPELVPELPHHLYTYHGCGLGALFTPEQARAILAARLNSLCQGFSGVSVALLEQITG 138 V + P EL +L H CGLG F+ E A ++ RLN+L +G SG ++ L+ Q+ Sbjct: 72 VRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMILRLNTLLKGHSGATLELVRQLQF 131 Query: 139 LLQHDLLPQIPCEGSVGASGDLTPLSYLAAVLCGERDVWREGATVPAAQALAAAGMTPLR 198 + ++P IP +GS+GASGDL PLS+LA L GE V G + L PL Sbjct: 132 FINERIIPIIPQQGSLGASGDLAPLSHLALALIGEGKVLYRGEEKDSDDVLRELNRQPLN 191 Query: 199 LRPKEGLAIMNGTAVMTALACLAFDRARYLCQLATRITAMSSFTLDGNAHHFDATLFSAK 258 L+ KEGLA++NGT MTA +++ A L + I A++ +L+G + + S + Sbjct: 192 LQAKEGLALINGTQAMTAQGVISYIEAEDLGYQSEWIAALTHQSLNGIIDAYRHDVHSVR 251 Query: 259 PHPGQQQVAA----WLQ-RDLPCGQAHRNEKRLQDRYSVRCAPHVIGVLNDALPSLRQFI 313 Q VAA WL+ L QA E R+QD Y++RC P + G ++Q + Sbjct: 252 NFQEQINVAARMRDWLEGSTLTTRQA---EIRVQDAYTLRCIPQIHGASFQVFNYVKQQL 308 Query: 314 ENELNSANDNPLIDPDGER--VLHGGHFYGGHIAFAMDSMKTAVANIADLLDRQMALMVD 371 E E+N+ANDNPLI + V+ GG+F+G IAFA+D +K V+ +A++ +R++ +V+ Sbjct: 309 EFEMNAANDNPLIFEEANETFVISGGNFHGQPIAFALDHLKLGVSELANVSERRLERLVN 368 Query: 372 QRYNNGLPANLSGAQGARXQPINHGLKALQISASAWTAEALKLTMPASVFSRSTECHNQD 431 + N LPA LS G + G +Q +A++ +E L PASV S ++ + +D Sbjct: 369 PQLNGDLPAFLSPEPG-----LQSGAMIMQYAAASLVSENKTLAHPASVDSITSSANQED 423 Query: 432 KVSMGTIAARDCLRVLELTEQVAAALLITVRQGVWLR 468 VSMGT AAR +++E +V A + Q L+ Sbjct: 424 HVSMGTTAARHGYQIIENARRVLAIECVIALQAAELK 460 >AGl1825 [E] COG2986 Histidine ammonia-lyase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 514 Score = 246 bits (628), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 168/455 (36%), Positives = 246/455 (54%), Gaps = 28/455 (6%) Query: 66 IYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGALFTPEQARAILAARLNSLCQGF 125 +YG+ TG+G ++ + V L +L H CG+G R I+A +L SL +G Sbjct: 55 VYGINTGFGKLASIKIDAADVATLQRNLILSHCCGVGVPLPENVVRLIMALKLVSLGRGA 114 Query: 126 SGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLAAVLCGERDVWREGATVPA 185 SGV + L+ I +L+ ++P IP +GSVGASGDL PL+++AAV+ GE + + EGA +PA Sbjct: 115 SGVRLELVRLIEAMLEKGVIPVIPEKGSVGASGDLAPLAHMAAVMMGEGEAFYEGAPLPA 174 Query: 186 AQALAAAGMTPLRLRPKEGLAIMNGTAVMTALACLAFDRARYLCQLATRITAMSSFTLDG 245 +ALA AG+TP+ L KEGLA++NGT TALA RA Q A A+S+ G Sbjct: 175 GEALAKAGLTPVVLAAKEGLALINGTQTSTALALAGLFRAHRAAQAALITGALSTDAAMG 234 Query: 246 NAHHFDATLFSAKPHPGQQQVAAWLQRDLPCGQAHR-----NEKRLQDRYSVRCAPHVIG 300 ++ F + S + H GQ A L R+L G R ++R+QD Y +RC P V G Sbjct: 235 SSAPFHPDIHSLRGHKGQIDAGAAL-RNLLEGSEIRVSHIEGDERVQDPYCIRCQPQVDG 293 Query: 301 VLNDALPSLRQFIENELNSANDNPLIDPDGERVLHGGHFYGGHIAFAMDSMKTAVANIAD 360 D L + + +E E N+ DNPL+ D V+ GG+F+ +AFA D A+ I Sbjct: 294 ACLDLLRQVARTLEIEANAVTDNPLVLSD-NSVVSGGNFHAEPVAFAADQTALAICEIGA 352 Query: 361 LLDRQMALMVDQRYNNGLPANLSGAQGARXQPINHGLKALQISASAWTAEALKLTMPASV 420 + R++AL+VD + GLPA LS G +N GL +++++A +E ++ PASV Sbjct: 353 IAQRRVALLVDPALSYGLPAFLSKKPG-----LNSGLMIAEVTSAALMSENKQMAHPASV 407 Query: 421 FSRSTECHNQDKVSMGTIAARDCLRVLELTEQVAAALLI---TVRQGVWLRSQRQQVGDT 477 S T + +D VSM AR R+L +T+ + A L I + QGV LR Sbjct: 408 DSTPTSANQEDHVSMACHGAR---RLLPMTDNLFAILGIEALSAVQGVELRG-------- 456 Query: 478 PLQDLPAGRLAAMQAALSEDIAVVAEDRMLEPDLR 512 PL+ P L + A L + + EDR + PDL+ Sbjct: 457 PLKTSP--ELQKVIAVLRVVVPSLEEDRYMAPDLK 489