BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0343.p (233 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 122 9e-28 VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pa... 96 9e-20 YPO1828 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 90 4e-18 ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pat... 89 8e-18 ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 89 8e-18 >RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Rso Ralstonia solanacearum Length = 248 Score = 122 bits (305), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 66/180 (36%), Positives = 105/180 (58%), Gaps = 12/180 (6%) Query: 53 QLDAIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNEIIQHFRQIAVNFSTEVS 112 +L R+ R++GFA+G + G + GR +G ++ +++ ++Q R+ + Sbjct: 63 ELANARDEGRRDGFAQGFQDGFEQGRRQG-----EEDSRQIAALMQSVREA-------ID 110 Query: 113 QCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPEDA 172 Q + +A EL+ LAL LA+ L+ AL+A+PE +LP V + P PA LR+H +DA Sbjct: 111 QMNGSVAEELVGLALQLAQQFLRGALRAQPERILPLVREVLGDAPTAPAPAMLRVHADDA 170 Query: 173 ALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRLGESLSKQLDWL 232 L+R+ LG ELA GW + DA ++RGGC+V+TR + DAT TRW L +L + W+ Sbjct: 171 ELIRQALGTELAAAGWTLVVDAAIERGGCRVQTRFGETDATLQTRWAELTRALGRDTAWI 230 >VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Vch Vibrio cholerae Length = 270 Score = 95.5 bits (236), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 67/243 (27%), Positives = 123/243 (50%), Gaps = 33/243 (13%) Query: 11 MTPYQRWELASFHDEPEP---------EPEPLPEEEEFLEEPPPV--PQLTAEQLDAIRE 59 +TP QRW L + E +P +P +EP V + + E++ IR Sbjct: 23 VTP-QRWGLPDYGAESNKAAKQTAFNYDPGWIPN----FDEPEQVVEHEFSEEEIALIRT 77 Query: 60 AARQEGF----AEGREHGLQAGRSEGYVAGLQQGQQE------------VNEIIQHFRQI 103 AA+QEGF AEG + G + G++EG+ AG Q+GQ + + E ++ F + Sbjct: 78 AAQQEGFEAGQAEGYQQGFEQGKAEGFQAGHQEGQTQGYQDGVAEGQALIQEQVKTFMAL 137 Query: 104 AVNFSTEVSQCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPA 163 A F+ + + ++ +L+D+ L L K ++ ++ P+++L TV A+++ALP+ Sbjct: 138 ANQFAQPLDLLNAQVEKQLVDMVLALTKEVVHVEVQTNPQVILDTVKASVEALPIAGHAI 197 Query: 164 SLRLHPEDAALVRKHLGE-ELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRLG 222 +L+L+PED ++R+ GE E+ + W + + L RG Q+E + + R + + Sbjct: 198 TLKLNPEDVEIIRQAYGEQEIETRNWTLLSEPALSRGDVQIEAGESSVSYRMEERIRSVL 257 Query: 223 ESL 225 +S Sbjct: 258 KSF 260 >YPO1828 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Ype Yersinia pestis Length = 238 Score = 90.1 bits (222), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 66/226 (29%), Positives = 108/226 (47%), Gaps = 22/226 (9%) Query: 13 PYQRWELASFHDEPE-PEPEPLPE-----EEEFLEEPPPVPQ---LTAEQLDAIREAARQ 63 P+Q W L F + E P E +P+ E +E V + L QL+A ++ RQ Sbjct: 9 PWQPWSLKDFASQSEAPLSESMPDISLLFPNEPMEATAAVDEQQVLVNLQLEAEKQG-RQ 67 Query: 64 EGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE--------IIQHFRQIAVNFSTEVSQCS 115 +GFA+G LQ G +GY GL++G Q+ + H++ + +F + Sbjct: 68 QGFAKG----LQEGLDKGYQTGLEEGHQQALADAQQQLAPMTAHWQVMVTDFQNTLDTLD 123 Query: 116 EKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPEDAALV 175 +A L+ +AL AK ++ +L + IQ P+ LR++P+D A+V Sbjct: 124 SVIASRLVQIALAAAKQIIGQPAICDGTALLAQIQQMIQQEPMFAGKTQLRVNPDDLAIV 183 Query: 176 RKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 + LG L+ GW++ D+ + GGC+V +DA+ ATRW L Sbjct: 184 EQRLGSTLSLHGWRLLGDSQIHAGGCKVSAEEGDLDASLATRWHEL 229 >ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein EcZ Escherichia coli O157:H7 EDL933 Length = 235 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 69/230 (30%), Positives = 105/230 (45%), Gaps = 13/230 (5%) Query: 1 MRAKIIPKEEMTPYQRW---ELASFHDEPEPEPEPLPEEEEFL--EEPPPVPQLTAEQLD 55 M A IP + P++ W +LA P+ E P+ E EE + E P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTWTPDDLAP----PQAEFVPMVESEETIIEEAEPSLEQQLAQLQM 56 Query: 56 AIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTEV 111 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T + Sbjct: 57 QAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTL 116 Query: 112 SQCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPED 171 +A L+ +AL+ A+ ++ ++ + +Q P+ LR+HP+D Sbjct: 117 DALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDD 176 Query: 172 AALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 V LG L+ GW++ D L GGC+V +DA+ ATRW+ L Sbjct: 177 LQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226 >ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Ecs Escherichia coli O157:H7 Length = 235 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 69/230 (30%), Positives = 105/230 (45%), Gaps = 13/230 (5%) Query: 1 MRAKIIPKEEMTPYQRW---ELASFHDEPEPEPEPLPEEEEFL--EEPPPVPQLTAEQLD 55 M A IP + P++ W +LA P+ E P+ E EE + E P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTWTPDDLAP----PQAEFVPMVESEETIIEEAEPSLEQQLAQLQM 56 Query: 56 AIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTEV 111 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T + Sbjct: 57 QAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTL 116 Query: 112 SQCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPED 171 +A L+ +AL+ A+ ++ ++ + +Q P+ LR+HP+D Sbjct: 117 DALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDD 176 Query: 172 AALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 V LG L+ GW++ D L GGC+V +DA+ ATRW+ L Sbjct: 177 LQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226