BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0315.p (248 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0341 [NO] COG1261 Flagellar basal body P-ring biosynthesis pr... 130 2e-30 STM1173 [NO] COG1261 Flagellar basal body P-ring biosynthesis pr... 100 4e-21 flgA [NO] COG1261 Flagellar basal body P-ring biosynthesis prote... 94 4e-19 YPO1798 [NO] COG1261 Flagellar basal body P-ring biosynthesis pr... 92 2e-18 ZflgA [NO] COG1261 Flagellar basal body P-ring biosynthesis prot... 91 2e-18 >RSp0341 [NO] COG1261 Flagellar basal body P-ring biosynthesis protein Rso Ralstonia solanacearum Length = 295 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 68/175 (38%), Positives = 106/175 (60%) Query: 74 IDQRMMLPACLDPQAFMPNGARLWGRTSVGVKCVAPAPWTIYVRANVQVISDYLIAAVPL 133 +D R+ C + +P RL GR VGV+C +P W +V A +Q+ Y +AA L Sbjct: 121 VDPRVANQPCDQVELMLPPANRLRGRIQVGVRCRSPHAWAAWVPATIQISGTYYVAARQL 180 Query: 134 AQGQTISASDISRVRGDMSALPAGVITEESQVIGRVAGMSLRAGTPLRMDAIRNQRVVQQ 193 G+T+ D+ GD+SALP VI + + +GRV SL PLR +++R VQ Sbjct: 181 PPGKTLDMGDLEARTGDLSALPPSVILQPADAVGRVLLTSLAPNQPLRAESLRMPIAVQA 240 Query: 194 GQSVRVVSNGPGFQISTEARALSNANEGQMTQARTAAGQVVSGIAKAGGILEINY 248 GQ+V++V++G GFQ++++ RAL+ A GQ+ Q RTA G V+SGIA++ G++ + + Sbjct: 241 GQTVKLVADGGGFQVTSDGRALNQAAVGQVAQVRTANGNVISGIAQSAGVVAVQF 295 >STM1173 [NO] COG1261 Flagellar basal body P-ring biosynthesis protein Sty Salmonella typhimurium LT2 Length = 219 Score = 100 bits (248), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 57/181 (31%), Positives = 100/181 (55%), Gaps = 7/181 (3%) Query: 67 ITIQTDPIDQRMMLPACLDPQAFMPNGARLWGRTSVGVKCVAPAPWTIYVRANVQVISDY 126 +T+++ P +LP+C P M A+LWG +V +C A Y++ NVQ +Y Sbjct: 45 VTLRSSP----NLLPSCEQPAFSMTGSAKLWGNVNVVARC---ANEKRYLQVNVQATGNY 97 Query: 127 LIAAVPLAQGQTISASDISRVRGDMSALPAGVITEESQVIGRVAGMSLRAGTPLRMDAIR 186 + A P+A+G ++ ++++ RG + LP + + Q+ V+ L G P+++ IR Sbjct: 98 VAVAAPIARGGKLTPANVTLKRGRLDQLPPRTVLDIRQIQDAVSLRDLAPGQPVQLTMIR 157 Query: 187 NQRVVQQGQSVRVVSNGPGFQISTEARALSNANEGQMTQARTAAGQVVSGIAKAGGILEI 246 V+ GQ V+V++NG GF ++ E +A++NA Q + R +GQ+VSG + G + I Sbjct: 158 QAWRVKAGQRVQVIANGEGFSVNAEGQAMNNAAVAQNARVRMTSGQIVSGTVDSDGNILI 217 Query: 247 N 247 N Sbjct: 218 N 218 >flgA [NO] COG1261 Flagellar basal body P-ring biosynthesis protein Eco Escherichia coli K12 Length = 219 Score = 93.6 bits (231), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 64/197 (32%), Positives = 105/197 (53%), Gaps = 9/197 (4%) Query: 53 EQFLKAQTAGLPGSI--TIQTDPIDQRMMLPACLDPQAFMPNGARLWGRTSVGVKCVAPA 110 F AQ AG+ + +I+T P +LP C P M N +RLWG +V +C Sbjct: 29 HNFFSAQLAGVSDEVRVSIRTAP----NLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDK 84 Query: 111 PWTIYVRANVQVISDYLIAAVPLAQGQTISASDISRVRGDMSALPAGVITEESQVIGRVA 170 Y++ NVQ +Y++AA+P+A+G + A ++ RG + LP + + +Q++ ++ Sbjct: 85 R---YLQVNVQATGNYVVAAMPIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAIS 141 Query: 171 GMSLRAGTPLRMDAIRNQRVVQQGQSVRVVSNGPGFQISTEARALSNANEGQMTQARTAA 230 L P+++ R V+ GQ V V+++G GF + E +AL+NA Q + R + Sbjct: 142 LRDLSPDQPIQLTQFRQAWRVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVS 201 Query: 231 GQVVSGIAKAGGILEIN 247 GQVVSG+ A G + IN Sbjct: 202 GQVVSGVVDADGNILIN 218 >YPO1798 [NO] COG1261 Flagellar basal body P-ring biosynthesis protein Ype Yersinia pestis Length = 232 Score = 91.7 bits (226), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 53/166 (31%), Positives = 85/166 (51%), Gaps = 3/166 (1%) Query: 81 PACLDPQAFMPNGARLWGRTSVGVKCVAPAPWTIYVRANVQVISDYLIAAVPLAQGQTIS 140 P C P+ +P AR WG S+ V+C +++ VQV Y +A LA G+ IS Sbjct: 68 PQCDAPEITLPANARPWGNISLSVRCDG---LRRFIQTQVQVSGRYAVAVRRLAAGEKIS 124 Query: 141 ASDISRVRGDMSALPAGVITEESQVIGRVAGMSLRAGTPLRMDAIRNQRVVQQGQSVRVV 200 A DI +G + LP G + + G V+ + AG PL + +R +V+ GQ V V+ Sbjct: 125 AQDIQMKQGRLDTLPPGALLDAHFAQGAVSQRQINAGQPLTRNMLRRLWLVKAGQDVPVL 184 Query: 201 SNGPGFQISTEARALSNANEGQMTQARTAAGQVVSGIAKAGGILEI 246 + G GF +++ +A++N + + R +GQ+VSG G + I Sbjct: 185 AQGDGFNVNSNGKAMNNGAAQETIRVRMPSGQIVSGTVADDGTIRI 230 >ZflgA [NO] COG1261 Flagellar basal body P-ring biosynthesis protein EcZ Escherichia coli O157:H7 EDL933 Length = 219 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 72/240 (30%), Positives = 121/240 (50%), Gaps = 26/240 (10%) Query: 10 ALRHACAALALLAGGLLALSPASAQNDPTVPQRQDLQIVQQTAEQFLKAQTAGLPGS--I 67 A++ + A +A+L SP SA ++ T + F +Q AG+ + Sbjct: 3 AIKRSVAIIAIL------FSPLSAASNLT-----------SQLQTFFSSQLAGISDEVRV 45 Query: 68 TIQTDPIDQRMMLPACLDPQAFMPNGARLWGRTSVGVKCVAPAPWTIYVRANVQVISDYL 127 +I+T P +LP C P M N +RLWG +V +C Y++ NVQ +Y+ Sbjct: 46 SIRTAP----NLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKR---YLQVNVQATGNYV 98 Query: 128 IAAVPLAQGQTISASDISRVRGDMSALPAGVITEESQVIGRVAGMSLRAGTPLRMDAIRN 187 +AA+P+ +G + A ++ RG + LP + + +Q++ V+ L P+++ R Sbjct: 99 VAAMPIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQ 158 Query: 188 QRVVQQGQSVRVVSNGPGFQISTEARALSNANEGQMTQARTAAGQVVSGIAKAGGILEIN 247 V+ GQ V V+++G GF + E +AL+NA Q + R +GQVVSG+ A G + IN Sbjct: 159 AWRVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN 218