BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0221.p (354 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs4360 [M] COG0845 Membrane-fusion protein Ecs Escherichia coli... 283 4e-76 yhiI [M] COG0845 Membrane-fusion protein Eco Escherichia coli K12 282 7e-76 ZyhiI [M] COG0845 Membrane-fusion protein EcZ Escherichia coli O... 281 1e-75 PA5232 [M] COG0845 Membrane-fusion protein Pae Pseudomonas aerug... 267 2e-71 STM3587 [M] COG0845 Membrane-fusion protein Sty Salmonella typhi... 263 4e-70 >ECs4360 [M] COG0845 Membrane-fusion protein Ecs Escherichia coli O157:H7 Length = 355 Score = 283 bits (724), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 161/348 (46%), Positives = 231/348 (66%), Gaps = 6/348 (1%) Query: 2 QHPLFAGLLLVVLAAVGWTGWAWW--RDADAPAGLVSSNGRLEATEVDIGSRLGGRIRHM 59 +H + G+ L+ +AA+ AWW R A P G SNGR+EATEVDI S++ GRI + Sbjct: 6 RHLAWWGVGLLAVAAIV----AWWLLRPAGVPEGFAVSNGRIEATEVDIASKIAGRIDTI 61 Query: 60 LVDEGEAVQAGQVLAYLQVDSLHAQLREARAQQQRASQGVTVAQAQLAMRRSEQRSAQAL 119 LV EG+ V+ G+VLA + L Q EA AQ + A V AQA L R+SE R+AQ+L Sbjct: 62 LVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVAAAQALLEQRQSETRAAQSL 121 Query: 120 TIQRHKSLSAATRRVERSQPLGRMQALNAQEVDDEENALAEARAQLDAAQAQEEGARAAI 179 QR L + +R RS+ L + A++AQ++DD+ A ARA L++A+AQ ++AAI Sbjct: 122 VNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAAESARAALESAKAQVSASKAAI 181 Query: 180 RAAEADVASARALLEVAQAATARLEADLEESAIRAPRDGHIQYRLAQAGEVVSEGSKLLN 239 AA ++ A+ +E AQA R+ AD+++S ++APRDG +QYR+A+ GEV++ G ++LN Sbjct: 182 EAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPRDGRVQYRVAEPGEVLAAGGRVLN 241 Query: 240 LIDLSDLHMHFFLPAELGGKVRLGQEVRVVLDAAPTDALPARITYVSRQAQFTPKAVETA 299 ++DLSD++M FFLP E G ++LG E R++LDAAP +PA I++V+ AQFTPK VET+ Sbjct: 242 MVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAPDLRIPATISFVASVAQFTPKTVETS 301 Query: 300 SERQKLMFRVKAQLTPSPRGSIAPHLKAGMPGVAWIKTAEQLPWPKTL 347 ER KLMFRVKA++ P ++K G+PGVAW++ E+LPWP L Sbjct: 302 DERLKLMFRVKARIPPELLQQHLEYVKTGLPGVAWVRVNEELPWPDDL 349 >yhiI [M] COG0845 Membrane-fusion protein Eco Escherichia coli K12 Length = 355 Score = 282 bits (722), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 156/327 (47%), Positives = 220/327 (67%), Gaps = 2/327 (0%) Query: 23 AWW--RDADAPAGLVSSNGRLEATEVDIGSRLGGRIRHMLVDEGEAVQAGQVLAYLQVDS 80 AWW R A P G SNGR+EATEVDI S++ GRI +LV EG+ V+ G+VLA + Sbjct: 23 AWWLLRPAGVPEGFAVSNGRIEATEVDIASKIAGRIDTILVKEGKFVREGEVLAKMDTRV 82 Query: 81 LHAQLREARAQQQRASQGVTVAQAQLAMRRSEQRSAQALTIQRHKSLSAATRRVERSQPL 140 L Q EA AQ + A V AQA L R+SE R+AQ+L QR L + +R RS+ L Sbjct: 83 LQEQRLEAIAQIKEAQSAVAAAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSL 142 Query: 141 GRMQALNAQEVDDEENALAEARAQLDAAQAQEEGARAAIRAAEADVASARALLEVAQAAT 200 + A++AQ++DD+ A ARA L++A+AQ ++AAI AA ++ A+ +E AQA Sbjct: 143 AQRGAISAQQLDDDRAAAESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATE 202 Query: 201 ARLEADLEESAIRAPRDGHIQYRLAQAGEVVSEGSKLLNLIDLSDLHMHFFLPAELGGKV 260 R+ AD+++S ++APRDG +QYR+A+ GEV++ G ++LN++DLSD++M FFLP E G + Sbjct: 203 RRIAADIDDSELKAPRDGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTL 262 Query: 261 RLGQEVRVVLDAAPTDALPARITYVSRQAQFTPKAVETASERQKLMFRVKAQLTPSPRGS 320 +LG E R++LDAAP +PA I++V+ AQFTPK VET+ ER KLMFRVKA++ P Sbjct: 263 KLGGEARLILDAAPDLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQ 322 Query: 321 IAPHLKAGMPGVAWIKTAEQLPWPKTL 347 ++K G+PGVAW++ E+LPWP L Sbjct: 323 HLEYVKTGLPGVAWVRVNEELPWPDDL 349 >ZyhiI [M] COG0845 Membrane-fusion protein EcZ Escherichia coli O157:H7 EDL933 Length = 355 Score = 281 bits (720), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 160/348 (45%), Positives = 230/348 (66%), Gaps = 6/348 (1%) Query: 2 QHPLFAGLLLVVLAAVGWTGWAWW--RDADAPAGLVSSNGRLEATEVDIGSRLGGRIRHM 59 +H + G+ L+ +AA+ WW R A P G SNGR+EATEVDI S++ GRI + Sbjct: 6 RHLAWWGVGLLAVAAIV----XWWLLRPAGVPEGFAVSNGRIEATEVDIASKIAGRIDTI 61 Query: 60 LVDEGEAVQAGQVLAYLQVDSLHAQLREARAQQQRASQGVTVAQAQLAMRRSEQRSAQAL 119 LV EG+ V+ G+VLA + L Q EA AQ + A V AQA L R+SE R+AQ+L Sbjct: 62 LVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVAAAQALLEQRQSETRAAQSL 121 Query: 120 TIQRHKSLSAATRRVERSQPLGRMQALNAQEVDDEENALAEARAQLDAAQAQEEGARAAI 179 QR L + +R RS+ L + A++AQ++DD+ A ARA L++A+AQ ++AAI Sbjct: 122 VNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAAESARAALESAKAQVSASKAAI 181 Query: 180 RAAEADVASARALLEVAQAATARLEADLEESAIRAPRDGHIQYRLAQAGEVVSEGSKLLN 239 AA ++ A+ +E AQA R+ AD+++S ++APRDG +QYR+A+ GEV++ G ++LN Sbjct: 182 EAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPRDGRVQYRVAEPGEVLAAGGRVLN 241 Query: 240 LIDLSDLHMHFFLPAELGGKVRLGQEVRVVLDAAPTDALPARITYVSRQAQFTPKAVETA 299 ++DLSD++M FFLP E G ++LG E R++LDAAP +PA I++V+ AQFTPK VET+ Sbjct: 242 MVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAPDLRIPATISFVASVAQFTPKTVETS 301 Query: 300 SERQKLMFRVKAQLTPSPRGSIAPHLKAGMPGVAWIKTAEQLPWPKTL 347 ER KLMFRVKA++ P ++K G+PGVAW++ E+LPWP L Sbjct: 302 DERLKLMFRVKARIPPELLQQHLEYVKTGLPGVAWVRVNEELPWPDDL 349 >PA5232 [M] COG0845 Membrane-fusion protein Pae Pseudomonas aeruginosa Length = 357 Score = 267 bits (683), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 151/339 (44%), Positives = 229/339 (67%) Query: 9 LLLVVLAAVGWTGWAWWRDADAPAGLVSSNGRLEATEVDIGSRLGGRIRHMLVDEGEAVQ 68 L++ L VG W R G S NGR+EATEVD+ ++L GR+ + VDEG+ V+ Sbjct: 13 LIVAALLGVGVLVWQVSRPTGLGEGFASGNGRIEATEVDVAAKLPGRVAEIKVDEGDFVK 72 Query: 69 AGQVLAYLQVDSLHAQLREARAQQQRASQGVTVAQAQLAMRRSEQRSAQALTIQRHKSLS 128 AG+++A + L AQL +A+AQ ++A A + +A R SE+ +AQA+ QR L+ Sbjct: 73 AGEIVARMDTQVLEAQLAQAQAQVRQAENAKLTATSLVAQRESEKSTAQAVVAQRQAELT 132 Query: 129 AATRRVERSQPLGRMQALNAQEVDDEENALAEARAQLDAAQAQEEGARAAIRAAEADVAS 188 AA +R R++ L + AL Q++DD+ L A+A L AA++Q A+AAI A + V Sbjct: 133 AAQKRFTRTEALVKRNALPQQQLDDDRATLQSAQAALSAARSQVISAQAAIEAGRSQVIE 192 Query: 189 ARALLEVAQAATARLEADLEESAIRAPRDGHIQYRLAQAGEVVSEGSKLLNLIDLSDLHM 248 A++ +E A+A+ ARL+AD+++S ++APR+G +QYR+AQ GEV+ G KLLN++DL+D++M Sbjct: 193 AQSAIEAAKASVARLQADIDDSLLKAPRNGRVQYRVAQPGEVLPAGGKLLNMVDLADVYM 252 Query: 249 HFFLPAELGGKVRLGQEVRVVLDAAPTDALPARITYVSRQAQFTPKAVETASERQKLMFR 308 FFLP+ G+V LGQEVR+V+DA P +PA+++YV+ AQFTPK VETA+ER+KLMFR Sbjct: 253 TFFLPSMQAGRVGLGQEVRLVIDAVPDYVIPAKVSYVASVAQFTPKTVETANEREKLMFR 312 Query: 309 VKAQLTPSPRGSIAPHLKAGMPGVAWIKTAEQLPWPKTL 347 VKA+L P+ ++K G+PG+A+++ ++ WP L Sbjct: 313 VKARLDPALLEKYITYVKTGVPGMAYLRLDPEVEWPANL 351 >STM3587 [M] COG0845 Membrane-fusion protein Sty Salmonella typhimurium LT2 Length = 355 Score = 263 bits (673), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 156/340 (45%), Positives = 225/340 (66%), Gaps = 7/340 (2%) Query: 10 LLVVLAAVGWTGWAWW--RDADAPAGLVSSNGRLEATEVDIGSRLGGRIRHMLVDEGEAV 67 +LV +AA+ AWW R A P G +SNGR+EATEVDI +++ GRI +LV EG+ V Sbjct: 15 ILVAVAAI-----AWWMLRPAGIPEGFAASNGRIEATEVDIATKIAGRIDTILVSEGQFV 69 Query: 68 QAGQVLAYLQVDSLHAQLREARAQQQRASQGVTVAQAQLAMRRSEQRSAQALTIQRHKSL 127 + G+VLA + L Q EA AQ + A V A+A L R+SE R+AQ++ QR L Sbjct: 70 RQGEVLAKMDTRVLQEQRLEAIAQIKEAESAVAAARALLEQRQSEMRAAQSVVKQREAEL 129 Query: 128 SAATRRVERSQPLGRMQALNAQEVDDEENALAEARAQLDAAQAQEEGARAAIRAAEADVA 187 + ++R RS+ L + A++ Q++DD+ A ARA L+ A+AQ A+AAI AA + Sbjct: 130 DSVSKRHVRSRSLSQRGAVSVQQLDDDRAAAESARAALETAKAQVSAAKAAIEAARTSII 189 Query: 188 SARALLEVAQAATARLEADLEESAIRAPRDGHIQYRLAQAGEVVSEGSKLLNLIDLSDLH 247 A+ +E AQA R+ AD+++S ++APRDG +QYR+A+ GEV+S G ++LN++DLSD++ Sbjct: 190 QAQTRVEAAQATERRIVADIDDSELKAPRDGRVQYRVAEPGEVLSAGGRVLNMVDLSDVY 249 Query: 248 MHFFLPAELGGKVRLGQEVRVVLDAAPTDALPARITYVSRQAQFTPKAVETASERQKLMF 307 M FFLP E G +++G E R+VLDAAP +PA I++V+ AQFTPK VET ER KLMF Sbjct: 250 MTFFLPTEQAGLLKIGGEARLVLDAAPDLRIPATISFVASVAQFTPKTVETHDERLKLMF 309 Query: 308 RVKAQLTPSPRGSIAPHLKAGMPGVAWIKTAEQLPWPKTL 347 RVKA++ P ++K G+PG+AW++ E++PWP L Sbjct: 310 RVKARIPPELLRQHLEYVKTGLPGMAWVRLDERVPWPDAL 349