BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0137.p (219 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1168 [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanine... 320 2e-87 VNG1311G [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanin... 128 8e-30 MYPU_0950_1 Non COG 115 5e-26 BH1749 [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanine ... 114 2e-25 DR2584 [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanine ... 113 3e-25 >RSc1168 [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase Rso Ralstonia solanacearum Length = 291 Score = 320 bits (819), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 148/197 (75%), Positives = 167/197 (84%) Query: 17 PAYWEEAKAELMKRDRIMRKIIPQFGDLQLTVRGDAFTTLARSVIGQQISTKAANAVWQR 76 PAYW+EA A+LMKRDRI+RKIIP FG L RGD F TLARS++GQQIS KAA +VW+R Sbjct: 90 PAYWQEACADLMKRDRILRKIIPAFGPAHLASRGDPFVTLARSIVGQQISVKAAQSVWER 149 Query: 77 FLEACPRCTPGQVIRTGPEGLAGCGLSKRKAEYILDLAAHFKAKTVHPDKWAEMEDEAVI 136 +EACP+ P Q +R G E LAGCGLSKRKAEYILDLA HF+ VH KWAEMEDE VI Sbjct: 150 LVEACPKLVPAQFLRAGHEKLAGCGLSKRKAEYILDLAEHFRNGMVHVAKWAEMEDEDVI 209 Query: 137 AEMIQIRGIGRWTAEMFLIFNLLRPNVLPLDDLGLLKGISISYFSGEPVSRSDAREVAAN 196 AE+ QIRGIGRWTAEMFL+FNLLRPNVLPLDD+GL+ IS +YFSGEPV+RS+AREVAAN Sbjct: 210 AELTQIRGIGRWTAEMFLMFNLLRPNVLPLDDIGLINAISQNYFSGEPVTRSEAREVAAN 269 Query: 197 WEPWRTVATWYLWRSLD 213 WEPWRTVATWY+WRSLD Sbjct: 270 WEPWRTVATWYMWRSLD 286 >VNG1311G [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase Hbs Halobacterium sp. NRC-1 Length = 191 Score = 128 bits (322), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 66/188 (35%), Positives = 108/188 (57%), Gaps = 3/188 (1%) Query: 26 ELMKRDRIMRKIIPQFGDLQLTVRGDAFTTLARSVIGQQISTKAANAVWQRFLEACPRCT 85 + ++ D + ++ G+L +T D F L ++I QQ+ST AA + +R +A T Sbjct: 6 DALRTDPTLAPLLDAHGELAVTPHDDLFERLVVAIIRQQLSTDAAATIRERVFDAVD-VT 64 Query: 86 PGQVIRTGPEGLAGCGLSKRKAEYILDLAAHFKAKTVHPDKWAEMEDEAVIAEMIQIRGI 145 P + P L GLS++K +Y+ ++AA F+ ++AE+ D AV E+ I G+ Sbjct: 65 PAGIAAADPAVLRDAGLSRQKTDYVTNVAAAFREHDYTRARFAELSDAAVREELTAITGV 124 Query: 146 GRWTAEMFLIFNLLRPNVLPLDDLGLLKGISISYFSGEPVSRSDAREVAANWEPWRTVAT 205 G WTA MFL+F L R +V P+ DLG+ G+ Y G +R++ RE+A +W P+R+ A+ Sbjct: 125 GDWTASMFLLFALGRADVFPVGDLGIRTGMQALY--GADTTRAEMREIADSWRPYRSYAS 182 Query: 206 WYLWRSLD 213 Y+WR+ D Sbjct: 183 LYVWRAQD 190 >MYPU_0950_1 Non COG Length = 217 Score = 115 bits (289), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 60/194 (30%), Positives = 101/194 (52%), Gaps = 4/194 (2%) Query: 19 YWEEAKAE---LMKRDRIMRKIIPQFGDLQLTVRGDAFTTLARSVIGQQISTKAANAVWQ 75 Y+E ++ E L ++D+ + K I + G ++ ++ + F + S+IGQQISTKA +W+ Sbjct: 2 YFEYSENEVNYLKRKDKKLAKAIEKIGFIKREIKPNLFVAIVHSIIGQQISTKAHKTLWK 61 Query: 76 RFLEACPRCTPGQVIRTGPEGLAGCGLSKRKAEYILDLAAHFKAKTVHPDKWAEMEDEAV 135 RF E + G G++ RK YILD A K D M D Sbjct: 62 RFEEKVKNIDAKTLASFDLFDFDGIGIANRKLTYILDFAKKVYNKEFDIDALYSMSDNEA 121 Query: 136 IAEMIQIRGIGRWTAEMFLIFNLLRPNVLPLDDLGLLKGISISYFSGEPVSRSDAREVAA 195 I + ++GIG+WTAEM +IF++ R N+L DL +++ I + Y + V + ++ Sbjct: 122 IKSLSSLKGIGQWTAEMAMIFSMQRKNILSYKDLAIIRAIKMLY-GYKDVDKITFEKLEK 180 Query: 196 NWEPWRTVATWYLW 209 + P+ ++A+ YLW Sbjct: 181 RYSPFASIASLYLW 194 >BH1749 [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase Bha Bacillus halodurans Length = 221 Score = 114 bits (284), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 65/195 (33%), Positives = 105/195 (53%), Gaps = 2/195 (1%) Query: 27 LMKRDRIMRKIIPQFGDLQLTVRGDAFTTLARSVIGQQISTKAANAVWQRF--LEACPRC 84 ++ +D + + I G++QL + + F +L S++ QQ+S KAA+A++ R L Sbjct: 14 IVAQDSRLFQFIEIAGEVQLPTKPNPFQSLVSSIVEQQLSIKAASAIYGRVEQLVGGALE 73 Query: 85 TPGQVIRTGPEGLAGCGLSKRKAEYILDLAAHFKAKTVHPDKWAEMEDEAVIAEMIQIRG 144 P Q+ R E L G+SKRK EYI + H ++ + + E VI ++ I+G Sbjct: 74 KPEQLYRVSDEALRQAGVSKRKIEYIRHVCEHVESGRLDFTELEGAEATTVIEKLTAIKG 133 Query: 145 IGRWTAEMFLIFNLLRPNVLPLDDLGLLKGISISYFSGEPVSRSDAREVAANWEPWRTVA 204 IG+WTAEMF++F+L R +VL + D+GL +G Y +GE + W P+ TVA Sbjct: 134 IGQWTAEMFMMFSLGRLDVLSVGDVGLQRGAKWLYGNGEGDGKKLLIYHGKAWAPYETVA 193 Query: 205 TWYLWRSLDPLPSDY 219 YLW++ +Y Sbjct: 194 CLYLWKAAGTFAEEY 208 >DR2584 [L] COG0122 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase Dra Deinococcus radiodurans Length = 225 Score = 113 bits (283), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 79/214 (36%), Positives = 115/214 (53%), Gaps = 12/214 (5%) Query: 1 LNTNRKAS--DVDPSLIVPAYWEEAKA-ELMKRDRIMRKIIPQFGDLQ-LTVRGDAFTTL 56 L+ NR+ + V ++P + A A + RD ++ ++ G+L L D F L Sbjct: 6 LHQNRRVTLPSVPLPAVLPPLTDHAGAVAHLSRDPVLAQVTSLCGELPVLAPTPDPFGRL 65 Query: 57 ARSVIGQQISTKAANAVWQRFLEACPR-CTPGQVIRTGPEGLAGCGLSKRKAEYILDLAA 115 RSV GQQ+S KAA A++ R LE P P +++ + L G GLS K + AA Sbjct: 66 VRSVAGQQLSVKAAQAIYGR-LEGLPGGVVPAALLKVSGDDLRGVGLSWAKVRTVQAAAA 124 Query: 116 HFKAKTVHPDKWAEMEDEAVIAEMIQIRGIGRWTAEMFLIFNLLRPNVLPLDDLGLLKGI 175 + + + DE VIAE++Q+ GIGRWTAEMFL+F L RP+V DL L +G+ Sbjct: 125 AAVSGQIDFAHLSGQPDELVIAELVQLPGIGRWTAEMFLLFALARPDVFSSGDLALRQGV 184 Query: 176 SISYFSGEPVSRSDAREVAANWEPWRTVATWYLW 209 + GE D R+V A W P+R++A+ YLW Sbjct: 185 E-RLYPGE-----DWRDVTARWAPYRSLASRYLW 212