BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0003.0085.p (217 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr9216 Non COG 95 1e-19 MTH1205 [C] COG2055 Malate/L-lactate dehydrogenases Mth Methanot... 83 4e-16 PH1277 [C] COG2055 Malate/L-lactate dehydrogenases Pho Pyrococcu... 77 3e-14 mlr9117 Non COG 76 7e-14 CAC0566 [C] COG2055 Malate/L-lactate dehydrogenases Cac Clostrid... 75 9e-14 >mlr9216 Non COG Length = 366 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 67/202 (33%), Positives = 100/202 (49%), Gaps = 7/202 (3%) Query: 12 QSVAPFGGTQAVFTPNPIAAGIPTS-GTPFLVDISASITTNGMSNRLHKAGKQFEEEWLI 70 VAPFGG +A NPI+ G+P++ PFL+D++ S G ++ E WLI Sbjct: 162 DCVAPFGGREARLGTNPISIGVPSNLQAPFLLDMATSAAAAGKLEVAIARREKIPEGWLI 221 Query: 71 DGAGHPSRDPAVLSATPPGTILPLGGLSAGHKGFGLAVLIEALTGGLS--GFGRADPAAG 128 D G P+ +P G +LPLGG G+KG GLA ++E L G L GFG P+ Sbjct: 222 DAEGRPTTNPH--DYLKGGVLLPLGG-PEGYKGSGLAAIVELLCGLLPDPGFG-PQPSGR 277 Query: 129 WGATVFMSLYDPSAFGGEQDFKRQMDHIAQACRANPPRPGVGQVRMPGDRGMQRRAEQLA 188 FM++++ +AF FK+++ A+ ++ P G V PG+ G R E++A Sbjct: 278 ENLGCFMAVFNVAAFCPLNKFKQEVAEFARYLKSATPSEGSPGVFYPGEIGYLRAQERMA 337 Query: 189 QGVTLHPTIAPMLREAGAAYDL 210 G+ + LR Y L Sbjct: 338 NGIEIEKATWDKLRTLARNYRL 359 >MTH1205 [C] COG2055 Malate/L-lactate dehydrogenases Mth Methanothermobacter thermautotrophicus Length = 341 Score = 83.2 bits (204), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 63/218 (28%), Positives = 102/218 (46%), Gaps = 25/218 (11%) Query: 2 MILASSDPAVQSVAPFGGTQAVFTPNPIAAGIPTSGTPFLVDISASITTNGMSNRLHKAG 61 +++A+++PAV AP GG + + NP+A GIP++ VD++ S + G + G Sbjct: 135 VVIANTEPAV---APIGGRKPILGTNPVAIGIPSNRYYVSVDMATSASARGKLLEAARKG 191 Query: 62 KQFEEEWLIDGAGHPSRDPAVLSATPPGTILPLGGLSAGHKGFGLAVLIEALTGGLSG-- 119 + E +D G P+ DP + G+ILP G GHKG+ L+ +IE L G L G Sbjct: 192 ESIPENVALDAEGKPTTDPEM---ALKGSILPFG----GHKGYALSFMIEILAGPLVGAA 244 Query: 120 -----FGRADPAAGWGATVFMSLYDPSAFGGEQDFKRQMDHIAQACRANPPRPGVGQVRM 174 G A+P M DPS +F+ Q+D + +++ G V + Sbjct: 245 FGTAVTGTANPEEMCTKGDLMMAIDPSKMVDPDEFRAQVDEFIEEVKSS------GDVLI 298 Query: 175 PGDRGMQRRAEQLAQGVTLHPTIAPMLREAGAAYDLEF 212 PGD + A+G+ L + + R G A +L+ Sbjct: 299 PGDIESMNIKRRRAEGIELDEKL--LERILGIARELDI 334 >PH1277 [C] COG2055 Malate/L-lactate dehydrogenases Pho Pyrococcus horikoshii Length = 360 Score = 77.0 bits (188), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 51/204 (25%), Positives = 94/204 (46%), Gaps = 6/204 (2%) Query: 14 VAPFGGTQAVFTPNPIAAGIPTSGTPFLVDISASITTNGMSNRLHKAGKQFEEEWLIDGA 73 VAP GG + + NPIA PT PFL+D++ S+ G + GK E W I+ Sbjct: 155 VAPTGGIERILGTNPIALAAPTKDKPFLLDMATSVVPIGKLEVYRRKGKDIPEGWAINRE 214 Query: 74 GH-PSRDPAVLSATPPGTILPLGGLSAGHKGFGLAVLIEALTGGLSG-----FGRADPAA 127 G+ ++ V + + G L GHKG+GL+++++ L+G LSG + + Sbjct: 215 GNITTKVEEVFNGGALLPLGGFGELLGGHKGYGLSLMVDILSGILSGGTWSKYVKNTSEK 274 Query: 128 GWGATVFMSLYDPSAFGGEQDFKRQMDHIAQACRANPPRPGVGQVRMPGDRGMQRRAEQL 187 G F + D F ++FK ++ + + +++ P ++ + G++G +L Sbjct: 275 GSNVCHFFMVIDIEHFIPLEEFKEKISQMIEEIKSSRKHPEFERIWIHGEKGFLTMETRL 334 Query: 188 AQGVTLHPTIAPMLREAGAAYDLE 211 G+ ++ + L E +E Sbjct: 335 KLGIPIYRKVLEELNEIAKRVGVE 358 >mlr9117 Non COG Length = 383 Score = 75.9 bits (185), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 61/189 (32%), Positives = 87/189 (46%), Gaps = 10/189 (5%) Query: 14 VAPFGGTQAVFTPNPIAAGIPTSG-TPFLVDISASITTNGMSNRLHKAGKQFEEEWLIDG 72 VAPFGG + + P GIP G P ++D + S+ G GK+ + LID Sbjct: 169 VAPFGGVERRMSTAPYCVGIPRQGHDPIVLDFATSVVAEGKCLVASLGGKKLPKGALIDS 228 Query: 73 AGHPSRDPAVL-----SATPPGTILPLGGLSA--GHKGFGLAVLIEALTGGLSGFGRADP 125 G S DP +L S + +G + A HKG GLA++ E + G L+G G A Sbjct: 229 DGTLSDDPRLLYGPYTSDSTRDHTQGIGAIRAFGEHKGSGLALICELMGGALTGTG-ATA 287 Query: 126 AAGWGATVFMSLY-DPSAFGGEQDFKRQMDHIAQACRANPPRPGVGQVRMPGDRGMQRRA 184 A A ++ Y DP F ++ RA P PG+ QV++PGD + RA Sbjct: 288 ANQRFANGMLAFYIDPKVVDPANFFDGEVTRYTDFIRATKPVPGIEQVQLPGDPERRIRA 347 Query: 185 EQLAQGVTL 193 E+ G+ L Sbjct: 348 ERTKDGIPL 356 >CAC0566 [C] COG2055 Malate/L-lactate dehydrogenases Cac Clostridium acetobutylicum Length = 369 Score = 75.5 bits (184), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 51/214 (23%), Positives = 95/214 (44%), Gaps = 12/214 (5%) Query: 14 VAPFGGTQAVFTPNPIAAGIPTSGTPFLVDISASITTNGMSNRLHKAGKQFEEEWLIDGA 73 V P G + + NPI+ +P PFL+D++ S+ T G K ++ W +D Sbjct: 154 VLPTYGREQMLGTNPISIAMPARPIPFLLDMATSVITRGKVEVYSKNNQKLNYGWAMDDE 213 Query: 74 GHPSRDP---AVLSATPPGTILPLGG---LSAGHKGFGLAVLIEALTGGLSGFGRADPAA 127 G + +P L+ G +LPLGG + GHKG+G++ +E T LSG +D Sbjct: 214 GRKTDNPDKVLKLANGNTGGLLPLGGDTEIFGGHKGYGISFAVEMFTSILSGGLTSDEIR 273 Query: 128 GWGAT-----VFMSLYDPSAFGGEQDFKRQMDHIAQACRANPPRPGVGQVRMPGDRGMQR 182 G T F ++ D F + +++M Q R + G ++ G++ ++ Sbjct: 274 GNNNTNGVCHAFAAI-DYGLFDDKNAIEKRMSAYMQKIRNSKKAYGASRIYTHGEKELEA 332 Query: 183 RAEQLAQGVTLHPTIAPMLREAGAAYDLEFPATL 216 +++ G+ ++ ++E D++ L Sbjct: 333 YEDRMENGIPINENTIVEIKEICDYVDIDINNYL 366