BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0004.p (464 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1105 [G] COG0380 Trehalose-6-phosphate synthase Rso Ralstonia... 457 e-128 SMa0233 [G] COG0380 Trehalose-6-phosphate synthase Sme Sinorhizo... 442 e-124 RSp0731 [G] COG0380 Trehalose-6-phosphate synthase Rso Ralstonia... 413 e-115 STM1928 [G] COG0380 Trehalose-6-phosphate synthase Sty Salmonell... 388 e-107 otsA [G] COG0380 Trehalose-6-phosphate synthase Eco Escherichia ... 382 e-105 >RSp1105 [G] COG0380 Trehalose-6-phosphate synthase Rso Ralstonia solanacearum Length = 465 Score = 457 bits (1177), Expect = e-128, Method: Compositional matrix adjust. Identities = 242/470 (51%), Positives = 310/470 (65%), Gaps = 17/470 (3%) Query: 1 MGRLVVVSNRLAD--PRKLAAGGLAVALGETLKKTGGLWFGWSGKVIENGTPG-EGEVRV 57 M RL+VVSNR+A + +AGGLAV + + L+ TGG+WFGWSG++ PG GE + Sbjct: 1 MSRLIVVSNRVAPIAEGQASAGGLAVGVYDALRDTGGVWFGWSGEI----APGASGEPSI 56 Query: 58 NQAGPVTLAELDLSQEDHDAYYRGYANGVLWPVFHYRLDLAHFEAGHLGGYRRVNQLFAR 117 G +T A + LS+ D+D YYRG+AN LWPVFHYR+DLA +E GYRRVN FA Sbjct: 57 AAKGAITFATVGLSRRDYDQYYRGFANATLWPVFHYRVDLARYERQEYHGYRRVNTQFAH 116 Query: 118 KLFPLLKRDDIVWIHDYHLIPLAAELRAMGCEARIGFFLHIPLPPPPILTAIPSHEWLMR 177 +L L++ DDI+W+HDYHLIP AAE RA+G RIGFFLHIP P P ILT IP HE LMR Sbjct: 117 QLKALVQPDDILWVHDYHLIPFAAECRALGLRNRIGFFLHIPFPSPEILTTIPPHEELMR 176 Query: 178 ALFAYDLVGFQNHADLQHFAQYVQAEA-----TAEPVGPNQFRAFNRTVHANAFPIGIDV 232 AL AYDL+GFQ D F Y++ EA E GP A+ T+ A +PIG+ Sbjct: 177 ALCAYDLLGFQTETDRVAFYDYIEREARGYIENKEHNGP--VHAYGNTLRAEVYPIGVHP 234 Query: 233 AEFQRLGQAKEARDTYESTKAEYSRRR---LLIGVDRLDYSKGLPQRIRAFRELLRLYPE 289 E R + AR + +A+ S R L++ VDRLDYSKGLP+R RAF +LL +P+ Sbjct: 235 DEIARQAVSSLARRNPFAREADASGGRPLKLIMSVDRLDYSKGLPERFRAFEQLLDDFPD 294 Query: 290 NRHSATLLQIASPSREDVEAYGDIQRELESLCGSINGDFGDFDWMPIRYIHRTVARRRLP 349 +R T +QIA SR+DV++Y I++ LE+ G ING + DW PIRYI++ RR L Sbjct: 295 HRRHVTFVQIAPTSRQDVQSYQQIRQRLEAESGRINGKHSELDWTPIRYINKQYDRRMLM 354 Query: 350 GLYRACAVALVTPLRDGMNLVAKEFVAAQDPDDPGVLVLSRFAGAAEQMKAALIINPYDT 409 L+RA + VTPLRDGMNLVAKE+VAAQDP+ PGVLVLSRFAGAA ++ AALI+NPYDT Sbjct: 355 ALFRASHIGYVTPLRDGMNLVAKEYVAAQDPEAPGVLVLSRFAGAARELDAALIVNPYDT 414 Query: 410 HGTAQVIQQALQMSQSERRERHAALMENIRQFDVHWWCREFLDTLRSSRA 459 G A+ + +AL M ER+ RHA +M+ +R D+ W FL LR + A Sbjct: 415 RGMAEALNRALTMPLEERKARHAHMMDRLRAADLTAWRERFLADLRGASA 464 >SMa0233 [G] COG0380 Trehalose-6-phosphate synthase Sme Sinorhizobium meliloti Length = 463 Score = 442 bits (1137), Expect = e-124, Method: Compositional matrix adjust. Identities = 229/460 (49%), Positives = 295/460 (64%), Gaps = 5/460 (1%) Query: 1 MGRLVVVSNRLADPRK---LAAGGLAVALGETLKKTGGLWFGWSGKVIENGTPGEGEVRV 57 M RLV+VSNR+ P K AGGLAVAL L++ GG+W GWSG+ P E ++ Sbjct: 1 MSRLVIVSNRVPVPDKGGIAPAGGLAVALKVALEEHGGIWMGWSGRSSGENEP-EPLAQL 59 Query: 58 NQAGPVTLAELDLSQEDHDAYYRGYANGVLWPVFHYRLDLAHFEAGHLGGYRRVNQLFAR 117 +Q G +T A DL+ D YY G+AN VLWP+ HYRLDLA + + GY RVN+ FA Sbjct: 60 HQ-GNITYALTDLTDTDVGEYYHGFANRVLWPICHYRLDLAEYGRKEMAGYFRVNRFFAH 118 Query: 118 KLFPLLKRDDIVWIHDYHLIPLAAELRAMGCEARIGFFLHIPLPPPPILTAIPSHEWLMR 177 +L PL++ DD++W+HDYHLIPLAAELR MG + RIGFFLHIP PP +L +P HE +MR Sbjct: 119 RLAPLVRPDDVIWVHDYHLIPLAAELRQMGLKNRIGFFLHIPWPPADVLFTMPVHEEIMR 178 Query: 178 ALFAYDLVGFQNHADLQHFAQYVQAEATAEPVGPNQFRAFNRTVHANAFPIGIDVAEFQR 237 L YD+VGFQ DL++FA ++ E + +G +F A+ R + IGI+ A F Sbjct: 179 GLSHYDVVGFQTDHDLENFAGCLRREGIGDELGGGRFSAYGRVFKGGIYAIGIETAAFAE 238 Query: 238 LGQAKEARDTYESTKAEYSRRRLLIGVDRLDYSKGLPQRIRAFRELLRLYPENRHSATLL 297 + T + R L+IGVDRLDYSKG+ QRI AF + P + T L Sbjct: 239 FAKKALTNKTVRKARESIEHRSLIIGVDRLDYSKGITQRIDAFERFILANPAQQGRVTYL 298 Query: 298 QIASPSREDVEAYGDIQRELESLCGSINGDFGDFDWMPIRYIHRTVARRRLPGLYRACAV 357 QI SR +V Y +QR + G +NG G DW+PIRYI+R+V R L GLYR V Sbjct: 299 QITPKSRSEVPEYEAMQRTVAEQAGRVNGALGAVDWVPIRYINRSVGRHILAGLYRLGKV 358 Query: 358 ALVTPLRDGMNLVAKEFVAAQDPDDPGVLVLSRFAGAAEQMKAALIINPYDTHGTAQVIQ 417 LVTPLRDGMNLVAKE+VAAQDPDDPGVLVLSRFAGAA +K AL++NPYD GTA + Sbjct: 359 GLVTPLRDGMNLVAKEYVAAQDPDDPGVLVLSRFAGAARDLKGALLVNPYDIEGTANAMA 418 Query: 418 QALQMSQSERRERHAALMENIRQFDVHWWCREFLDTLRSS 457 +AL M ER++R +M+++ + DV WCR+FL+ L +S Sbjct: 419 RALSMPLEERKDRWKTMMDHLLEHDVSRWCRDFLNDLATS 458 >RSp0731 [G] COG0380 Trehalose-6-phosphate synthase Rso Ralstonia solanacearum Length = 557 Score = 413 bits (1062), Expect = e-115, Method: Compositional matrix adjust. Identities = 217/452 (48%), Positives = 285/452 (63%), Gaps = 9/452 (1%) Query: 3 RLVVVSNRLADPRKLAAGGLAVALGETLKKTGGLWFGWSGKVIENGTPGEGEVRVNQAGP 62 RLVVVSNRL DP++ AAGGLAVALG+ ++ T GLWFGWSGK ++ P VR G Sbjct: 80 RLVVVSNRLIDPQRPAAGGLAVALGDMMRDTDGLWFGWSGKTVDE--PDTACVRTEPFGR 137 Query: 63 VTLAELDLSQEDHDAYYRGYANGVLWPVFHYRLDLAHFEAGHLGGYRRVNQLFARKLFPL 122 TLA++DL + D++ YY G++N VLWP+FH R+ A + Y VN++ A L P+ Sbjct: 138 TTLAQVDLGRTDYEGYYAGFSNSVLWPIFHERVKWADLNPAYFKAYEAVNRILASHLRPM 197 Query: 123 LKRDDIVWIHDYHLIPLAAELRAMGCEARIGFFLHIPLPPPPILTAIPSHEWLMRALFAY 182 L+ DI+W+HDYHLIP A LRA+GC R+GFF HIPLP P ++ IP H LMRAL +Y Sbjct: 198 LRDSDILWVHDYHLIPFAQALRALGCTQRMGFFNHIPLPSPDVIRRIPQHRQLMRALASY 257 Query: 183 DLVGFQNHADLQHFAQYVQAEATAEPVGPNQFRAFNRTVHANAFPIGIDVAEFQRLGQAK 242 DLVG Q+ D+ + +Y AE A Q + +VHA FPIGIDV + L + Sbjct: 258 DLVGMQSPRDVLNLQRYWDAENIAA-----QSERRHDSVHA--FPIGIDVESLRALVPSP 310 Query: 243 EARDTYESTKAEYSRRRLLIGVDRLDYSKGLPQRIRAFRELLRLYPENRHSATLLQIASP 302 ++ + + R L+IGVDRLDYSKG+P R++AFR+LL+ + R TL+QIA+P Sbjct: 311 ASQAVIDEVRGAAGRCVLMIGVDRLDYSKGIPTRLKAFRQLLQTHARMRSKVTLVQIAAP 370 Query: 303 SREDVEAYGDIQRELESLCGSINGDFGDFDWMPIRYIHRTVARRRLPGLYRACAVALVTP 362 +R+ + AY ++ + E L IN FG DW P+ Y +V R LP LYR V +VTP Sbjct: 371 TRQSIPAYARLRDKTEQLVREINRRFGTGDWTPVMYFSESVDRTALPQLYRMSRVGVVTP 430 Query: 363 LRDGMNLVAKEFVAAQDPDDPGVLVLSRFAGAAEQMKAALIINPYDTHGTAQVIQQALQM 422 + DGMNLVAKE+VAAQ DPGVLVLSR AGAA Q++ AL++ + TA QAL M Sbjct: 431 VADGMNLVAKEYVAAQAARDPGVLVLSRGAGAASQLRDALLVPSKNRAATADAYAQALSM 490 Query: 423 SQSERRERHAALMENIRQFDVHWWCREFLDTL 454 ER+ RHA LM NI D+ WW +L L Sbjct: 491 PLEERKARHAGLMRNIETEDLRWWRDNYLKAL 522 >STM1928 [G] COG0380 Trehalose-6-phosphate synthase Sty Salmonella typhimurium LT2 Length = 473 Score = 388 bits (997), Expect = e-107, Method: Compositional matrix adjust. Identities = 212/456 (46%), Positives = 280/456 (61%), Gaps = 6/456 (1%) Query: 1 MGRLVVVSNRLADP-RKLAAGGLAVALGETLKKTGGLWFGWSGKVIENGTPGEGEVRVNQ 59 M RLVVVSNR+A P K AGGLAV + LK GGLWFGWSG E G E +V + Sbjct: 1 MSRLVVVSNRIAPPDNKGGAGGLAVGVLGALKAAGGLWFGWSG---ETGNEDEPLKKVTK 57 Query: 60 AGPVTLAELDLSQEDHDAYYRGYANGVLWPVFHYRLDLAHFEAGHLGGYRRVNQLFARKL 119 G +T A +LS++D++ YY ++N VLWP FHYRLDL F+ GY RVN L A KL Sbjct: 58 -GNITWASFNLSEQDYEDYYCQFSNAVLWPAFHYRLDLVQFQRPAWEGYMRVNALLADKL 116 Query: 120 FPLLKRDDIVWIHDYHLIPLAAELRAMGCEARIGFFLHIPLPPPPILTAIPSHEWLMRAL 179 PL+K +DI+W+HDYHL+P A+ELR G RIGFFLHIP P P I A+P H+ L+ L Sbjct: 117 LPLIKENDIIWVHDYHLLPFASELRKRGVNNRIGFFLHIPFPTPEIFNALPPHDELLEQL 176 Query: 180 FAYDLVGFQNHADLQHFAQYVQAEATAEPVGPNQFRAFNRTVHANAFPIGIDVAEFQRLG 239 +DL+GFQ D F + ++ Q A+ + +PIGI+ E L Sbjct: 177 CDFDLLGFQTENDRLAFLDSLSSQTRVTTRSGKQHIAWGKDFQTEVYPIGIEPDEIA-LQ 235 Query: 240 QAKEARDTYESTKAEYSRRRLLIGVDRLDYSKGLPQRIRAFRELLRLYPENRHSATLLQI 299 A KAE + + V+RLDYSKGLP+R A+ LL YP++R QI Sbjct: 236 AAGPLPPKLAQLKAELKNVKNIFSVERLDYSKGLPERFLAYEALLENYPQHRGKIRYTQI 295 Query: 300 ASPSREDVEAYGDIQRELESLCGSINGDFGDFDWMPIRYIHRTVARRRLPGLYRACAVAL 359 A SR +V+AY DI+ +LE+ G ING +G W P+ Y+++ R+ L ++R V L Sbjct: 296 APTSRGEVQAYQDIRHQLETEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGL 355 Query: 360 VTPLRDGMNLVAKEFVAAQDPDDPGVLVLSRFAGAAEQMKAALIINPYDTHGTAQVIQQA 419 VTPLRDGMNLVAKEFVAAQDP +PGVLVLS+FAGAA ++ +ALI+NPYD A + +A Sbjct: 356 VTPLRDGMNLVAKEFVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDDVAAALNRA 415 Query: 420 LQMSQSERRERHAALMENIRQFDVHWWCREFLDTLR 455 L M +ER RHA +++ I + D++ W F+ L+ Sbjct: 416 LTMPLAERISRHAEMLDVIVKNDINRWQERFIHDLK 451 >otsA [G] COG0380 Trehalose-6-phosphate synthase Eco Escherichia coli K12 Length = 474 Score = 382 bits (980), Expect = e-105, Method: Compositional matrix adjust. Identities = 212/466 (45%), Positives = 278/466 (59%), Gaps = 7/466 (1%) Query: 1 MGRLVVVSNRLADPRKLAA--GGLAVALGETLKKTGGLWFGWSGKVIENGTPGEGEVRVN 58 M RLVVVSNR+A P + AA GGLAV + LK GGLWFGWSG+ P ++ Sbjct: 1 MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQP----LKKV 56 Query: 59 QAGPVTLAELDLSQEDHDAYYRGYANGVLWPVFHYRLDLAHFEAGHLGGYRRVNQLFARK 118 + G +T A +LS++D D YY ++N VLWP FHYRLDL F+ GY RVN L A K Sbjct: 57 KKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNALLADK 116 Query: 119 LFPLLKRDDIVWIHDYHLIPLAAELRAMGCEARIGFFLHIPLPPPPILTAIPSHEWLMRA 178 L PLL+ DDI+WIHDYHL+P A ELR G RIGFFLHIP P P I A+P+++ L+ Sbjct: 117 LLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQ 176 Query: 179 LFAYDLVGFQNHADLQHFAQYVQAEATAEPVGPNQFRAFNRTVHANAFPIGIDVAEFQRL 238 L YDL+GFQ D F + A+ + +PIGI+ E + Sbjct: 177 LCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQ 236 Query: 239 GQAKEARDTYESTKAEYSRRRLLIGVDRLDYSKGLPQRIRAFRELLRLYPENRHSATLLQ 298 A KAE + + V+RLDYSKGLP+R A+ LL YP++ Q Sbjct: 237 A-AGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQ 295 Query: 299 IASPSREDVEAYGDIQRELESLCGSINGDFGDFDWMPIRYIHRTVARRRLPGLYRACAVA 358 IA SR DV+AY DI+ +LE+ G ING +G W P+ Y+++ R+ L ++R V Sbjct: 296 IAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVG 355 Query: 359 LVTPLRDGMNLVAKEFVAAQDPDDPGVLVLSRFAGAAEQMKAALIINPYDTHGTAQVIQQ 418 LVTPLRDGMNLVAKE+VAAQDP +PGVLVLS+FAGAA ++ +ALI+NPYD A + + Sbjct: 356 LVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDR 415 Query: 419 ALQMSQSERRERHAALMENIRQFDVHWWCREFLDTLRSSRAAPVES 464 AL MS +ER RHA +++ I + D++ W F+ L+ ES Sbjct: 416 ALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAES 461